scholarly journals Resistant Sources and Genetic Control of Resistance to ToLCNDV in Cucumber

2021 ◽  
Vol 9 (5) ◽  
pp. 913
Author(s):  
Cristina Sáez ◽  
Laura G. M. Ambrosio ◽  
Silvia M. Miguel ◽  
José Vicente Valcárcel ◽  
María José Díez ◽  
...  
Keyword(s):  
Genetic Control ◽  
High Load ◽  
F1 Hybrids ◽  
New Delhi ◽  
Chromosome 2 ◽  
Mechanical Inoculation ◽  
Symptom Development ◽  
Tissue Printing ◽  
Inheritance Model ◽  
Tomato Leaf Curl

Tomato leaf curl New Delhi virus (ToLCNDV) is a severe threat for cucurbit production worldwide. Resistance has been reported in several crops, but at present, there are no described accessions with resistance to ToLCNDV in cucumber (Cucumis sativus). C. sativus var. sativus accessions were mechanically inoculated with ToLCNDV and screened for resistance, by scoring symptom severity, tissue printing, and PCR (conventional and quantitative). Severe symptoms and high load of viral DNA were found in plants of a nuclear collection of Spanish landraces and in accessions of C. sativus from different geographical origins. Three Indian accessions (CGN23089, CGN23423, and CGN23633) were highly resistant to the mechanical inoculation, as well as all plants of their progenies obtained by selfing. To study the inheritance of the resistance to ToLCNDV, plants of the CGN23089 accession were crossed with the susceptible accession BGV011742, and F1 hybrids were used to construct segregating populations (F2 and backcrosses), which were mechanically inoculated and evaluated for symptom development and viral load by qPCR. The analysis of the genetic control fit with a recessive monogenic inheritance model, and after genotyping with SNPs distributed along the C. sativus genome, a QTL associated with ToLCNDV resistance was identified in chromosome 2 of cucumber.

scholarly journals Virus titer as a disease resistance indicator in tomato

2019 ◽  
Vol 48 (3) ◽  
pp. 609-617
Author(s):  
Sajid Ali ◽  
Ammara Fatima ◽  
Sana Khalid ◽  
Farah Saeed ◽  
Muhammad Shafiq ◽  
...  
Keyword(s):  
Large Scale ◽  
Virus Titer ◽  
Yield Potential ◽  
New Delhi ◽  
Symptom Development ◽  
Economic Traits ◽  
High Level ◽  
Tomato Leaf Curl ◽  
The Relationship ◽  
Major Reduction

Tomato leaf curl new Delhi virus, a geminivirus with a wide host range is a contagious pathogen of tomato, which also infects many other crops and weeds. Whitefly (Bemisia tabaci Gennadius), a polyphagous vector, is the agent responsible for its spread on a large scale. The pathogen is responsible for a major reduction in the yield of tomato. Ten commercial cultivars of tomato plant were selected to evaluate the effect of virus titer on crop yield. The yield potential along with other traits of these cultivars was assessed on the basis of symptom development, and virus DNA accumulation. The relationship between the virus titer, symptom severity, and agro-economic traits were established. The present results explain that the high level of virus accumulation in plant tissue results in the development of severe symptoms and leads to a major reduction in yield in case of susceptible cultivars, but this is not true for the cultivars showing intermediate resistance. The virus DNA remains low and approximately constant in resistant cultivars and has minimal effect on the yield and health of tomato.

scholarly journals Supervirulent pseudorecombination and asymmetric synergism between genomic components of two distinct species of begomovirus associated with severe tomato leaf curl disease in India

2008 ◽  
Vol 89 (3) ◽  
pp. 818-828 ◽  
Author(s):  
S. Chakraborty ◽  
R. Vanitharani ◽  
B. Chattopadhyay ◽  
C. M. Fauquet
Keyword(s):  
Distinct Species ◽  
Tomato Leaf ◽  
New Delhi ◽  
Symptom Development ◽  
Tomato Leaf Curl Disease ◽  
Northern India ◽  
Viral Dnas ◽  
Tomato Leaf Curl ◽  
Leaf Curl Disease ◽  
Leaf Curl

Isolates of two distinct begomovirus species, the severe strain of the species Tomato leaf curl New Delhi virus (tomato leaf curl New Delhi virus-[India:New Delhi:Severe:1992]; ToLCNDV-[IN:ND:Svr:92], bipartite) and the Varanasi strain of the species Tomato leaf curl Gujarat virus (tomato leaf curl Gujarat virus-[India:Varanasi:2001]; ToLCGV-[IN:Var:01], mono/bipartite) infect tomato (Lycopersicon esculentum) and cause severe yield losses in northern India. This study investigated the infectivity properties of genomic components of these two species. Both pseudorecombinants were infectious in Nicotiana benthamiana, Nicotiana tabacum and L. esculentum. Enhanced pathogenicity was observed when DNA-A of ToLCNDV-[IN:ND:Svr:92] was trans-complemented with ToLCGV-[IN:Var:01] DNA-B, and was consistently associated with an increase in accumulation of ToLCGV-[IN:Var:01] DNA-B. Mixed infection of ToLCNDV-[IN:ND:Svr:92] and ToLCGV-[IN:Var:01] always showed extremely severe symptoms, suggesting a synergistic interaction between these two viruses. Southern blot analysis of viral DNAs from infected plants showed a significantly higher level of accumulation of both ToLCNDV components and DNA-B of ToLCGV-[IN:Var:01] with no alteration to levels of DNA-A of ToLCGV-[IN:Var:01]. Symptom development and/or higher infectivity of the supervirulent pseudorecombinants correlated with the increased levels of DNA-B accumulation. Protoplast replication assays revealed that enhanced infectivity by the pseudorecombinant occurred at the level of replication, as DNA-A of ToLCNDV-[IN:ND:Svr:92] enhanced ToLCGV-[IN:Var:01] DNA-B replication, whose accumulation was in turn increased by ToLCGV-[IN:Var:01] DNA-A. This is the first report demonstrating a virulent pseudorecombinant between two distinct species of begomoviruses that infect tomato, and is the second report on synergism between begomoviruses. The results revealed that ToLCGV-[IN:Var:01] DNA-B is capable of associating with different DNA-A components, despite having different iteron sequences.

Potato apical leaf curl disease: current status and perspectives on a disease caused by tomato leaf curl New Delhi virus

2021 ◽  
Author(s):  
Ravinder Kumar ◽  
Rahul Kumar Tiwari ◽  
Arjunan Jeevalatha ◽  
Sundaresha Siddappa ◽  
Mohd. Abas Shah ◽  
...  
Keyword(s):  
Tomato Leaf ◽  
Current Status ◽  
New Delhi ◽  
Apical Leaf ◽  
Tomato Leaf Curl ◽  
Leaf Curl Disease ◽  
Leaf Curl

Tomato leaf curl New Delhi virus. [Distribution map].

2016 ◽  
Author(s):  
Keyword(s):  
Tamil Nadu ◽  
Uttar Pradesh ◽  
Tomato Leaf ◽  
New Delhi ◽  
Distribution Map ◽  
Tomato Leaf Curl ◽  
Virus Distribution ◽  
New Distribution ◽  
Distribution In Europe ◽  
Leaf Curl

Abstract A new distribution map is provided for Tomato leaf curl New Delhi virus. Geminiviridae: Begomovirus. Hosts: tomato (Solanum lycopersicum) and other Solanaceae such as aubergine (S. melongena), potato (S. tuberosum), Capsicum spp. and Cucurbitaceae. Information is given on the geographical distribution in Europe (Italy, Sicily, Spain, Mainland Spain), Asia (Bangladesh, India, Andhra Pradesh, Delhi, Gujarat, Haryana, Karnataka, Maharashtra, Punjab, Tamil Nadu, Uttar Pradesh, West Bengal, Indonesia, Java, Iran, Pakistan, Philippines, Sri Lanka, Taiwan and Thailand) and Africa (Tunisia).

Diagnostic methods of tomato leaf curl New Delhi virus in Russian Federation

2021 ◽  
Author(s):  
E. Lozovaya ◽  
Y. Prikhodko ◽  
T. Zhivaeva ◽  
E. Karimova ◽  
Y. Shneyder
Keyword(s):  
Russian Federation ◽  
Tomato Leaf ◽  
Diagnostic Methods ◽  
New Delhi ◽  
Tomato Leaf Curl ◽  
Leaf Curl

Emergence of tomato leaf curl New Delhi virus in Italy: estimation of incidence and genetic diversity

2019 ◽  
Vol 68 (3) ◽  
pp. 601-608 ◽  
Author(s):  
S. Panno ◽  
A. G. Caruso ◽  
E. Troiano ◽  
M. Luigi ◽  
A. Manglli ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Tomato Leaf ◽  
New Delhi ◽  
Tomato Leaf Curl ◽  
Leaf Curl

Diversity analysis of Tomato leaf curl New Delhi virus-[potato], causing apical leaf curl disease of potato in India

2017 ◽  
Vol 45 (1) ◽  
pp. 33-43 ◽  
Author(s):  
Arjunan Jeevalatha ◽  
Swarup Kumar Chakrabarti ◽  
Sanjeev Sharma ◽  
Vinay Sagar ◽  
Kamlesh Malik ◽  
...  
Keyword(s):  
Tomato Leaf ◽  
Diversity Analysis ◽  
New Delhi ◽  
Apical Leaf ◽  
Tomato Leaf Curl ◽  
Leaf Curl Disease ◽  
Leaf Curl

scholarly journals First report of tomato leaf curl New Delhi virus in zucchini crops in Greece

2019 ◽  
Vol 101 (3) ◽  
pp. 799-799 ◽  
Author(s):  
Chrysoula G. Orfanidou ◽  
Ioanna Malandraki ◽  
Despoina Beris ◽  
Oxana Kektsidou ◽  
Nikon Vassilakos ◽  
...  
Keyword(s):  
Tomato Leaf ◽  
New Delhi ◽  
First Report ◽  
Tomato Leaf Curl ◽  
Leaf Curl
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