scholarly journals Characterization of Soil Bacteria with Potential to Degrade Benzoate and Antagonistic to Fungal and Bacterial Phytopathogens

2021 ◽  
Vol 9 (4) ◽  
pp. 755
Author(s):  
Tatiana Z. Esikova ◽  
Tatiana O. Anokhina ◽  
Tatiana N. Abashina ◽  
Nataliya E. Suzina ◽  
Inna P. Solyanikova
Keyword(s):  
Crop Yields ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Antifungal Effect ◽  
Benzoate Degradation ◽  
Development Cycle ◽  
Microbial Strains ◽  
Agricultural Plants ◽  
Fungal Phytopathogens

The intensive development of agriculture leads to the depletion of land and a decrease in crop yields and in plant resistances to diseases. A large number of fertilizers and pesticides are currently used to solve these problems. Chemicals can enter the soil and penetrate into the groundwater and agricultural plants. Therefore, the primary task is to intensify agricultural production without causing additional damage to the environment. This problem can be partially solved using microorganisms with target properties. Microorganisms that combine several useful traits are especially valuable. The aim of this work was to search for new microbial strains, which are characterized by the ability to increase the bioavailability of nutrients, phytostimulation, the antifungal effect and the decomposition of some xenobiotics. A few isolated strains of the genera Bacillus and Pseudomonas were characterized by high activity against fungal phytopathogens. One of the bacterial strains identified as Priestiaaryabhattai on the basis of the 16S rRNA gene sequence was characterized by an unusual cellular morphology and development cycle, significantly different from all previously described bacteria of this genus. All isolated bacteria are capable of benzoate degradation as a sign of the ability to degrade aromatic compounds. Isolated strains were shown to be prospective agents in biotechnologies.

scholarly journals New Strains of Bacteria that Degrade Aromatic Compounds Act as Antagonists of Highly Active Phytopathogens

Proceedings ◽  
2020 ◽  
Vol 66 (1) ◽  
pp. 2
Author(s):  
Tatiana O. Anokhina ◽  
Tatiana Z. Esikova ◽  
Tatiana N. Abashina ◽  
Nataliya E. Suzina ◽  
Inna P. Solyanikova
Keyword(s):  
Aromatic Compounds ◽  
Crop Yields ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Antifungal Effect ◽  
Highly Active ◽  
Benzoate Degradation ◽  
Development Cycle ◽  
Microbial Strains ◽  
Fungal Phytopathogens

The intensive development of agriculture leads to the depletion of land and a decrease in crop yields and in plant resistance to diseases. A large number of fertilizers and pesticides are currently used to solve these problems. Chemicals can enter the soil and penetrate into the groundwater and agricultural plants. Therefore, the primary task is to intensify agricultural production without causing additional damage to the environment. This problem can be partially solved using microorganisms with target properties. Microorganisms that combine several useful traits are especially valuable. The aim of this work was to search for new microbial strains that possess a complex, technologically significant potential, such as increasing the bioavailability of nutrients, phytostimulation, antifungal effect and degradation of pesticide residues and other xenobiotics. Few isolated Bacillus and Pseudomonas strains were characterized by high activity against fungal phytopathogens. One of the bacterial strains identified as Bacillus sp. on the basis of the 16S rRNA gene sequence was characterized by an unusual cellular morphology and development cycle, significantly different from all previously described bacteria of this genus. All isolated bacteria are capable of benzoate degradation, as a sign of the ability to degrade aromatic compounds. Isolated strains were shown to be perspective agents in biotechnologies.

Ruegeria pelagia sp. nov., isolated from the Sargasso Sea, Atlantic Ocean

2007 ◽  
Vol 57 (8) ◽  
pp. 1815-1818 ◽  
Author(s):  
Kiyoung Lee ◽  
Yoe-Jin Choo ◽  
Stephen J. Giovannoni ◽  
Jang-Cheon Cho
Keyword(s):  
Phylogenetic Analyses ◽  
Cellular Fatty Acid ◽  
Rrna Gene ◽  
Sargasso Sea ◽  
Bacterial Strains ◽  
Ruegeria Pomeroyi ◽  
Distinct Line ◽  
The 16S Rrna Gene ◽  
Sequence Similarities

Gram-negative, facultatively aerobic, chemoheterotrophic, short rod-shaped marine bacterial strains HTCC2662T and HTCC2663, isolated from the Sargasso Sea by using a dilution-to-extinction culturing method, were investigated to determine their taxonomic position. Characterization of the two strains by phenotypic and phylogenetic analyses revealed that they belonged to the same species. The DNA G+C content of strain HTCC2662T was 58.4 mol% and the predominant cellular fatty acids were C18 : 1 ω7c (52.5 %), C16 : 0 2-OH (13.5 %) and C18 : 1 11-methyl ω7c (12.2 %). Phylogenetic analysis of the 16S rRNA gene sequences showed that the strains represented a distinct line of descent within the genus Ruegeria, with highest sequence similarities to Ruegeria atlantica DSM 5823T (97.2 %), Ruegeria lacuscaerulensis DSM 11314T (96.5 %) and Ruegeria pomeroyi DSM 15171T (95.6 %). Several phenotypic characteristics, including facultatively requiring NaCl and oxygen for growth, together with the cellular fatty acid composition, differentiated strain HTCC2662T from other members of the genus Ruegeria. Based on phenotypic, chemotaxonomic and phylogenetic traits, it is suggested that strains HTCC2662T and HTCC2663 represent a novel species of the genus Ruegeria, for which the name Ruegeria pelagia sp. nov. is proposed. The type strain is HTCC2662T (=KCCM 42378T=NBRC 102038T).

scholarly journals Characterization of the Polyurethanolytic Activity of Two Alicycliphilus sp. Strains Able To Degrade Polyurethane and N-Methylpyrrolidone

2007 ◽  
Vol 73 (19) ◽  
pp. 6214-6223 ◽  
Author(s):  
Alejandro Oceguera-Cervantes ◽  
Agustín Carrillo-García ◽  
Néstor López ◽  
Sandra Bolaños-Nuñez ◽  
M. Javier Cruz-Gómez ◽  
...  
Keyword(s):  
Esterase Activity ◽  
Maximum Growth ◽  
Maximal Activity ◽  
Gas Chromatography Mass Spectrometry ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Carbon And Nitrogen ◽  
Gene Sequence Analysis ◽  
Hyperbolic Behavior

ABSTRACT Two bacterial strains (BQ1 and BQ8) were isolated from decomposed soft foam. These were selected for their capacity to grow in a minimal medium (MM) supplemented with a commercial surface-coating polyurethane (PU) (Hydroform) as the carbon source (MM-PUh). Both bacterial strains were identified as Alicycliphilus sp. by comparative 16S rRNA gene sequence analysis. Growth in MM-PUh showed hyperbolic behavior, with BQ1 producing higher maximum growth (17.8 ± 0.6 mg·ml−1) than BQ8 (14.0 ± 0.6 mg·ml−1) after 100 h of culture. Nuclear magnetic resonance, Fourier transform infrared (IR) spectroscopy, and gas chromatography-mass spectrometry analyses of Hydroform showed that it was a polyester PU type which also contained N-methylpyrrolidone (NMP) as an additive. Alicycliphilus sp. utilizes NMP during the first stage of growth and was able to use it as the sole carbon and nitrogen source, with calculated K s values of about 8 mg·ml−1. Enzymatic activities related to PU degradation (esterase, protease, and urease activities) were tested by using differential media and activity assays in cell-free supernatants of bacterial cultures in MM-PUh. Induction of esterase activity in inoculated MM-PUh, but not that of protease or urease activities, was observed at 12 h of culture. Esterase activity reached its maximum at 18 h and was maintained at 50% of its maximal activity until the end of the analysis (120 h). The capacity of Alicycliphilus sp. to degrade PU was demonstrated by changes in the PU IR spectrum and by the numerous holes produced in solid PU observed by scanning electron microscopy after bacterial culture. Changes in the PU IR spectra indicate that an esterase activity is involved in PU degradation.

scholarly journals Characterization of Phascolarctobacterium succinatutens sp. nov., an Asaccharolytic, Succinate-Utilizing Bacterium Isolated from Human Feces

2011 ◽  
Vol 78 (2) ◽  
pp. 511-518 ◽  
Author(s):  
Yohei Watanabe ◽  
Fumiko Nagai ◽  
Masami Morotomi
Keyword(s):  
Bacterial Species ◽  
Short Chain Fatty Acids ◽  
Pcr Analysis ◽  
Rrna Gene ◽  
Gi Tract ◽  
Bacterial Strains ◽  
High Sequence Identity ◽  
Healthy Human ◽  
Content Type

ABSTRACTIsolation, cultivation, and characterization of the intestinal microorganisms are important for understanding the comprehensive physiology of the human gastrointestinal (GI) tract microbiota. Here, we isolated two novel bacterial strains, YIT 12067Tand YIT 12068, from the feces of healthy human adults. Phylogenetic analysis indicated that they belonged to the same species and were most closely related toPhascolarctobacterium faeciumACM 3679T, with 91.4% to 91.5% 16S rRNA gene sequence similarities, respectively. Substrate availability tests revealed that the isolates used only succinate; they did not ferment any other short-chain fatty acids or carbohydrates tested. When these strains were cocultured with the xylan-utilizing and succinate-producing bacteriumParaprevotella xylaniphilaYIT 11841T, in medium supplemented with xylan but not succinate, their cell numbers became 2 to 3 orders of magnitude higher than those of the monoculture; succinate became undetectable, and propionate was formed. Database analysis revealed that over 200 uncultured bacterial clones from the feces of humans and other mammals showed high sequence identity (>98.7%) to YIT 12067T. Real-time PCR analysis also revealed that YIT 12067T-like bacteria were present in 21% of human fecal samples, at an average level of 3.34 × 108cells/g feces. These results indicate that YIT 12067T-like bacteria are distributed broadly in the GI tract as subdominant members that may adapt to the intestinal environment by specializing to utilize the succinate generated by other bacterial species. The phylogenetic and physiological properties of YIT 12067Tand YIT 12068 suggest that these strains represent a novel species, which we have designatedPhascolarctobacterium succinatutenssp. nov.

scholarly journals Isolation and characterization of bacteria associated with silkworm gut under antibiotic-treated larval feeding

2024 ◽  
Vol 84 ◽  
Author(s):  
A. Javaid ◽  
M. Hussain ◽  
K. Aftab ◽  
M. F. Malik ◽  
M. Umar ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Close Relation ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Larval Weight ◽  
Silkworm Larvae ◽  
Cocoon Production ◽  
Isolation And Characterization

Abstract The impact of antibiotics on growth, cocoon production was assessed in addition to isolation and characterization of bacteria associated with silkworm gut of infected larvae. Larval rearing was maintained at recommended conditions of temperature and humidity. Silkworm larvae showing abnormal symptoms were collected from the control group and dissected for gut collection. Bacteria were isolated from the gut content by spreading on agar plates and incubated at 37 °C for 48 hrs. Bacterial identification and phylogenetic analysis were carried out by 16S rRNA gene sequencing. The isolated bacteria were subjected to antimicrobial susceptibility test (disc diffusion methods) by using Penicillin (10 µg/mL), Tetracycline (30 µg/mL), Amoxicillin (25 µg/mL), Ampicillin (10 µg/mL), and Erythromycin (15 µg/mL). All isolated strains showed positive results for the catalase test. We isolated and identified bacterial strains (n = 06) from the gut of healthy and diseased silkworm larvae. Based on the 16S rRNA gene sequence, isolated bacteria showed close relation with Serratia, Bacillus, and Pseudomonas spp. Notably, 83.3% of strains were resistant to Penicillin, Tetracycline, Amoxicillin, Ampicillin, and Erythromycin but 16.6% showed antibiotic susceptibility to the above-mentioned commonly used antibiotics. Silkworm larvae fed on penicillin-treated leaves showed significant improvement in larval weight, larval length, and cocoon production. Significantly higher larval weight (6.88g), larval length (5.84cm), and cocoon weight (1.33g) were recorded for larvae fed on leaves treated with penicillin as compared to other antibiotics. Isolated bacterial strains showed close relation with Serratia spp., Bacillus spp. and Pseudomonas spp.

Biochemical and molecular characterization of proteolytic bacterial strains isolated from Jazan region, KSA with the application as an antibacterial agent

2020 ◽  
Vol 3 ◽  
pp. 1
Author(s):  
Bander Mohammed Al-Thobaiti ◽  
Emad Abada ◽  
Khaled El-Gayar
Keyword(s):  
Enzyme Activity ◽  
Molecular Characterization ◽  
Bacterial Growth ◽  
Antibacterial Agent ◽  
Pathogenic Bacteria ◽  
Crude Enzyme ◽  
Genetic Identification ◽  
Rrna Gene ◽  
Bacterial Strains

Objectives: Biochemical and molecular characterization of proteolytic bacterial strains isolated from Jazan region, KSA with the application as an antibacterial agent. Materials and Methods: Three samples were collected from extreme environment, Jazan, KSA. Skim milk nutrient agar medium was used for protease screening for several colonies by streaking method at 37°C. API biochemical kit was used to characterize the three isolates using some selective media. The genetic identification was done using 16S rRNA gene sequencing. The sensitivity of the tested strains;Staphylococcus aureus, Escherichia coli, and Klebsiella pneumoniae against the extracellular crude protease enzyme produced from the three isolated bacteria and different antibiotics was done. Results: The isolates were identified as Bacillus subtilis, Bacillus licheniformis, and Bacillus cereus. B. cereus and B. licheniformis recorded high sensitivity (71%) against most antibiotics, in addition, B. subtilis showed resistance to Aztreonam only. It was found a considerable increase in the level of both of protease activity (units/ml) and bacterial growth (colony-forming units/ml) of the cultures that were directed by the B. subtilis and B. licheniformis up to 37°C then decreased at 45°C. On the contrary, the growth of B. cereus and its activity gradually increased up to 45°C. The enzyme activity and bacterial growth of B. subtilis and B. cereus strains were increased at alkaline medium. However, B. licheniformis gave the highest growth and activity at neutral pH. In addition, it was found that the enzyme activity and bacterial growth of B. subtilis were reached to the maximum at 5% NaCl. However, the maximum bacterial growth and enzyme activity for B. licheniformis and B. cereus was at 2% NaCl. It was found high effect on inhibiting the growth of pathogenic bacteria using 5 μl of crude enzyme with specific enzyme activity 73, 76, and 92 (units/ml)/(mg protein/ml) for B. subtilis, B. licheniformis, and B. cereus, respectively. All pathogenic bacteria were totally inhibited with 10 μl of crude enzyme. Conclusion: The potential Bacillus proteases can promote new industry as antimicrobial agents.

ANTIMICROBIAL AND ANTIRADICAL PROPERTIES OF HAMMADA SCOPARIA (POMEL) ILJIN

2020 ◽  
Vol 16 (2) ◽  
pp. 1-14
Author(s):  
Aziz Drioiche ◽  
Nadia Benhlima ◽  
Samira Kharchouf ◽  
Fadoua EL MAKHOUKHI ◽  
Smahane MEHANNED ◽  
...  
Keyword(s):  
Fungal Infections ◽  
Chemical Characterization ◽  
Traditional Healers ◽  
Bacterial Strains ◽  
Traditional Use ◽  
Antifungal Effect ◽  
Aerial Parts ◽  
Organic Extracts

Background: Hammada scoparia (Pomel) Iljin (HS), is commonly used by traditional healers in Morocco against microbial and fungal infections. We studied antimicrobial, antifungal and antiradical effects of organic extracts in vitro in order to confirm traditional utilization after phytochemical screening. Materials and methods: Aerial parts of HS have been extracted by hydro-distillation using Clevenger-type apparatus, and the chemical composition was realized by Gas Chromatography coupled with Mass Spectroscopy (GC/MS). The antioxidant activity has been evaluated using DPPH test, while the antimicrobial tests of HS extract were conducted on twenty-eight bacterial strains and antifungal on twelve fungal strains. Results: Chemical characterization of HS essential oils (EO) confirmed the presence of carvacrol (82,28%), p-cymene (2,52%), γ-terpinene (2,18%) and Z-caryophyllene (2,04%). Antimicrobial tests of HS extract showed a moderate antibacterial activity without antifungal effect. In addition, HS exhibited a very powerful antiradical activity with IC50 = 1,2 mg/ml compared to that of ascorbic acid (IC50 = 0,5 mg/mL) and butylated hydroxyanisole (0,8 mg/mL). Conclusion: To our knowledge, this is the first demonstration that HS directly inhibits the growth of microorganisms in vitro, and further validates its traditional use as an antiseptic by traditional Moroccan healers.

scholarly journals Degradation of 2,3,7,8-TCDD by a consortium of bacterial strains isolated from heavil herbicide/dioxin contaminated soil in Bienhoa airbase

2020 ◽  
Vol 16 (4) ◽  
pp. 777-784
Author(s):  
Pham Quang Huy ◽  
Nguyen Kim Thoa ◽  
Dang Thi Cam Ha
Keyword(s):  
Contaminated Soil ◽  
Large Scale ◽  
Morphological Characteristics ◽  
Bacterial Consortium ◽  
Soil Extract ◽  
High Ratio ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Agrobacterium Strain ◽  
Microbial Strains

From two different soil sources in Bienhoa airbase (heavy herbicide/dioxin contaminated West-South region and bioremediated cell), five microbial strains were isolated and their 2,3,7,8-TCDD biodegrability in consortium was investigated. Based on the colony and cell morphological characteristics as well as 16S rRNA gene sequences, these strains were classified into 5 genera, including Methylobacterium (strain BHBi1), Hydrocarboniphaga (strain BHBi4), Agrobacterium (strain BHBi5), Bosea (strain BHBi7) and Microbacterium (strain BH09). Two strains BHBi7 and BHBi4 were the first representatives of the genera Bosea and Hydrocarboniphaga that were isolated from heavyly herbicide/dioxin contaminated soil. All five strains were able to grow well in mineral salt medium (MSM) supplemented with soil extract (SE) containing 2,3,7,8-TCDD (this congener is the main soil total compound toxicity) and other congeners, including PCDDs, PCDFs, 2,4,5-T, 2,4-D, PAHs and their intermediates. This microbial consortium degraded 2,537.34 ngTEQ/kg of 2,3,7,8-TCDD congener in soil, equivalent to 59.1% lost of total toxicity in comparison to the control without bacterial seeding (4,294.12 ng TEQ/kg). Such a high ratio of dioxin degradation by a bacterial consortium was reported here for the first time, contributing more evidences for convincing the successful dioxin bioremediation of “Active Landfill” technology at large scale in Z1 area at Bienhoa airbase, Dongnai, Vietnam.

scholarly journals Characterization of six clinical isolates of Chimaeribacter gen. nov., a novel genus related to the Yersiniaceae family and the three species Chimaeribacter arupi sp. nov., Chimaeribacter coloradensis sp. nov, and Chimaeribacter californicus sp. nov.

2020 ◽  
Vol 70 (4) ◽  
pp. 2703-2712 ◽  
Author(s):  
Alessandro Rossi ◽  
Mark A. Fisher
Keyword(s):  
Type Strain ◽  
Single Species ◽  
Individual Species ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Gram Negative ◽  
Novel Genus ◽  
Content Type ◽  
Link Type

Eight genetically related, Gram-negative bacterial strains, isolated from clinical specimens between 2012 and 2016, were submitted to arup Laboratories for species identification. The lack of species- or genus-level matches in curated 16S rRNA gene databases prompted us to undertake the polyphasic characterization of these so far undescribed organisms. Six isolates available for additional testing were oxidase negative, catalase positive, pleomorphic, Gram-negative rods displaying temperature-dependent motility and producing yellow-pigmented colonies with three distinct morphotypes: medium-sized shiny, large mucoid and agar-pitting. Biochemical reactions and sugar fermentation patterns were most similar to members of the genus Serratia . Fatty acid profiles were highly similar across all six organisms, with the major components being: C16 : 0; C17 : 0 cyclo; C14 : 0 3-OH/iso-C16 : 1 I; C18 : 1 ω7c; and C16 : 1 ω7c/C16 : 1 ω6c. Whole-genome comparisons and multi locus sequence analysis (using the coding genes atpD, rpoB, gyrB and infB) suggest that the strains here described constitute three individual species within a novel genus related to the family Yersiniaceae . We propose for this novel taxon the name Chimaeribacter gen. nov., referring to the presentation of multiple characteristics typical of distinct Enterobacterales genera within a single organism. Four isolates are representative of a single species: Chimaeribacter arupi sp. nov (2016-Iso1, 2016-Iso2, type strain 2016-Iso3T=DSM 110101T=ATCC TSD-180T and 2013-Iso5). The remaining two isolates constitute the novel species Chimaeribacter coloradensis sp. nov. (type strain 2016-Iso4T=DSM 110102T=ATCC TSD-182T) and Chimaeribacter californicus sp. nov. (type strain 2015-Iso6T=DSM 110100T=ATCC TSD-181T). Our work provides the first formal characterization of the genus Chimaeribacter and forms the basis to study its taxonomic diversity.

scholarly journals Identification and molecular characterization of Chryseobacterium vrystaatense ST1 isolated from oligomineral water of southeast Serbia

2012 ◽  
Vol 64 (3) ◽  
pp. 877-883
Author(s):  
S. Tasic ◽  
M. Kojic ◽  
S. Stankovic ◽  
D. Obradovic
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Molecular Characterization ◽  
Universal Primers ◽  
Rrna Gene ◽  
Bacterial Strains ◽  
Biochemical Tests ◽  
First Time

The isolation and molecular characterization of bacterial strains isolated from water sources in the Vlasina Mountain in southeast Serbia, confirmed the presence of a new species Chryseobacterium vrystaatense ST1. This Gram- negative species showed an extremely low level of biochemical reactivity in biochemical tests. The gene for 16S rRNA was amplified by PCR using universal primers and sequenced. Comparison of 16S rRNA gene sequence and phenotypic features indicated that the isolate ST belonged to Chryseobacterium vrystaatense. A BLAST search of sequenced 1088 nucleotides of the 16S rRNA gene with all sequences deposited in the NCBI collection showed the highest similarity (98%) with the strain Chryseobacterium vrystaatense sp. nov., designated as strain R-23533. The very high homology of these two strains allowed classification of our strain at the species level, but some differences indicate, and indirectly confirm, that the isolate ST is an authentic representative. On the basis of these results, we could conclude that Chryseobacterium vrystaatense ST was for first time isolated in Serbia, which is particularly important when one bears in mind that there are only three sequences of this species deposited in the NCBI collection.

Sign in / Sign up

Export Citation Format

Share Document