scholarly journals Genital Brucella suis Biovar 2 Infection of Wild Boar (Sus scrofa) Hunted in Tuscany (Italy)

2021 ◽  
Vol 9 (3) ◽  
pp. 582
Author(s):  
Giovanni Cilia ◽  
Filippo Fratini ◽  
Barbara Turchi ◽  
Marta Angelini ◽  
Domenico Cerri ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Wild Boar ◽  
Sus Scrofa ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Central Italy ◽  
Brucella Species ◽  
Molecular Assays ◽  
Brucella Suis ◽  
Venereal Transmission

Brucellosis is a zoonosis caused by different Brucella species. Wild boar (Sus scrofa) could be infected by some species and represents an important reservoir, especially for B. suis biovar 2. This study aimed to investigate the prevalence of Brucella spp. by serological and molecular assays in wild boar hunted in Tuscany (Italy) during two hunting seasons. From 287 animals, sera, lymph nodes, livers, spleens, and reproductive system organs were collected. Within sera, 16 (5.74%) were positive to both rose bengal test (RBT) and complement fixation test (CFT), with titres ranging from 1:4 to 1:16 (corresponding to 20 and 80 ICFTU/mL, respectively). Brucella spp. DNA was detected in four lymph nodes (1.40%), five epididymides (1.74%), and one fetus pool (2.22%). All positive PCR samples belonged to Brucella suis biovar 2. The results of this investigation confirmed that wild boar represents a host for B.suis biovar. 2 and plays an important role in the epidemiology of brucellosis in central Italy. Additionally, epididymis localization confirms the possible venereal transmission.

scholarly journals Brucellosis in wild boar (Sus scrofa) in the Republic of Croati

2012 ◽  
Vol 49 (No. 4) ◽  
pp. 115-122 ◽  
Author(s):  
Z. Cvetnic ◽  
j. Toncic ◽  
s. Spicic ◽  
m. Lojkic ◽  
s. Terzic ◽  
...  
Keyword(s):  
Wild Boar ◽  
Sus Scrofa ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Agglutination Test ◽  
Tissue Samples ◽  
Blood Samples ◽  
Natural Vector ◽  
Brucella Suis ◽  
The Republic

During the years 2001 and 2002 on seven localities in Croatiaa survey on the prevalence of brucellosis in wild boar was carried out. The survey included 271 (52.7%) female and 243 (47.3%) male animals between 7 months and 4 years of age and weighing from 14 to 135 kg. On that occasion 514 blood samples of wild boar were serologically analysed. For serological analysis indirect enzyme immunoassay (iELISA), Rose Bengal test (RBT), complement fixation test (CFT) and slow agglutination test (SAT) were used. In all of the wild boar from all of the localities investigated positive reactions to brucellosis were established. Most of the positive reactions were established by iELISA (13.6%), then by RBT (11.5%), CFT (10.5%) and SAT (8.9%). Tissue samples of 106 animals: testes samples from 67 animals, uterus tissue from 38 animals and 5 fetuses of piglets from 1 mother were analysed bacteriologically. Brucella suis biovar 2 was isolated from 18 (17.0%) animals that originated from all of the localities investigated. Isolates were identified by PCR using BRU-UP and BRU-LOW primers specific for genus Brucella and primers specific for IS711. Based on our results it could be concluded that in Croatia wild boar are natural vector and/or reservoirs of B. suis biovar 2. This permanent risk factor is hazardious for domestic and wild animals in theRepublic ofCroatia.

scholarly journals Leptospira Survey in Wild Boar (Sus scrofa) Hunted in Tuscany, Central Italy

Pathogens ◽  
2020 ◽  
Vol 9 (5) ◽  
pp. 377 ◽  
Author(s):  
Giovanni Cilia ◽  
Fabrizio Bertelloni ◽  
Marta Angelini ◽  
Domenico Cerri ◽  
Filippo Fratini
Keyword(s):  
16S Rrna ◽  
Wild Boar ◽  
Sus Scrofa ◽  
Central Italy ◽  
23S Rrna ◽  
Rrna Gene ◽  
Pathogenic Leptospira ◽  
Molecular Assays ◽  
Realtime Pcr ◽  
23S Rrna Gene

Leptospirosis is a re-emerging, worldwide zoonosis, and wild boar (Sus scrofa) are involved in its epidemiology as the reservoir. The aim of this study was to investigate the prevalence of Leptospira with serological, bacteriological, and molecular assays in wild boar hunted in Tuscany (Italy) during two hunting seasons. In total, 287 specimens of sera, kidneys, and liver were collected to perform microscopic agglutination tests (MATs), isolation, and RealTime PCR to detect pathogenic (lipL32 gene), intermediate (16S rRNA gene), and saprophytic (23S rRNA gene) Leptospira. Within sera, 39 (13.59%) were positive to the MAT, and Australis was the most represented serogroup (4.88%), followed by Pomona (4.18%), and Tarassovi (3.14%). Moreover, four Leptospira cultures were positive, and once isolates were identified, one was identified as L. borgpetersenii serovar Tarassovi, and three as L. interrogans serovar Bratislava. Pathogenic Leptospira DNA were detected in 32 wild boar kidneys (11.15%). The characterization through the amplification of the rrs2 gene highlighted their belonging to L. interrogans (23 kidneys), L. borgpetersenii (four), and L. kirschneri (one), while nine kidneys (3.14%) were positive for intermediate Leptospira, all belonging to L. fainei. The results of this study confirmed the importance of wild boar in the epidemiology of leptospirosis among wildlife in Central Italy.

scholarly journals Seroprevalence and Molecular Identification of Brucella spp. in Camels in Egypt

2020 ◽  
Vol 8 (7) ◽  
pp. 1035 ◽  
Author(s):  
Aman Ullah Khan ◽  
Ashraf E. Sayour ◽  
Falk Melzer ◽  
Sherif Abdel Ghafar Elsayed El-Soally ◽  
Mandy C. Elschner ◽  
...  
Keyword(s):  
Complement Fixation Test ◽  
Indirect Elisa ◽  
Complement Fixation ◽  
Brucella Melitensis ◽  
Control Strategies ◽  
Effective Control ◽  
Brucella Species ◽  
Serum Samples ◽  
Brucella Suis ◽  
The Rose

Brucellosis is one of the most important worldwide zoonoses of many countries including Egypt. Camel brucellosis has not gained much attention in Egypt yet. This study is focused on the three governorates with the highest camel populations and the largest camel markets in the country to determine the disease seroprevalence and identify the Brucella species in local camel holdings. In total, 381 serum samples were collected from male and female camels from Giza, Aswan, and Al-Bahr Al-Ahmar (the Red Sea) governorates. Samples were serologically examined using the Rose–Bengal plate test (RBPT), indirect ELISA (i-ELISA), competitive ELISA (c-ELISA) and complement fixation test (CFT). Brucella antibodies were detected in 59 (15.5%), 87 (22.8%), 77 (20.2%) and 118 (31.0%) of sera by RBPT, i-ELISA, c-ELISA and CFT, respectively. Using real-time PCR, Brucella DNA was amplified in 32 (8.4%) seropositive samples including Brucella abortus (25/32), Brucella suis (5/32) and Brucella melitensis (2/32), defining a complex epidemiological status. To the best of our knowledge, this is the first study reporting Brucella suis DNA in camel serum. The risk-associated factors including age, sex, breed and geographical distribution were statistically analyzed, showing non-significant association with seroprevalence. The results of this study will raise awareness for camel brucellosis and help develop effective control strategies.

Gene expression changes in spleens of the wildlife reservoir species, Eurasian wild boar (Sus scrofa), naturally infected with Brucella suis biovar 2

2010 ◽  
Vol 37 (11) ◽  
pp. 725-736 ◽  
Author(s):  
Ruth C. Galindo ◽  
Pilar M. Muñoz ◽  
María J. de Miguel ◽  
Clara M. Marin ◽  
Javier Labairu ◽  
...  
Keyword(s):  
Gene Expression ◽  
Wild Boar ◽  
Sus Scrofa ◽  
Reservoir Species ◽  
Brucella Suis ◽  
Wildlife Reservoir

Brucella suis infection in domestic pigs in Sardinia (Italy)

2014 ◽  
Vol 143 (10) ◽  
pp. 2170-2177 ◽  
Author(s):  
C. PILO ◽  
M. T. TEDDE ◽  
G. ORRÙ ◽  
G. ADDIS ◽  
M. LICIARDI
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Bacteriological Examination ◽  
Chain Reaction ◽  
Serum Samples ◽  
Domestic Pigs ◽  
Brucella Suis ◽  
Commercial Farms ◽  
Polymerase Chain ◽  
The Rose

SUMMARYDuring a 4-year (2007–2010) survey, the presence of Brucella suis infection in domestic pigs in Sardinia was investigated. Serum samples were collected from breeding pigs located on 108 commercial farms with documented reproductive problems and analysed using the Rose Bengal (RBT) and complement fixation (CFT) tests for screening and confirmation of Brucella, respectively. Of the 1251 serum samples analysed by RBT, 406 sera, originating from 36 farms, were positive for B. suis. CFT was positive in 292/748 sera analysed, confirming positivity in all 36 pig herds. Pigs with international complement fixation test units per ml (ICFTU/ml) values ⩾160 were slaughtered, and their organs collected for bacteriological examination and testing by polymerase chain reaction (PCR). Brucella spp. strains were isolated in culture from 13/502 organs analysed, and subsequently identified as B. suis biovar 2. PCR detected positivity to Brucella spp. in 19/285 organs analysed. These results confirm the presence and emergence of B. suis infection in domestic pigs in Sardinia.

scholarly journals Monitoring results of wild boar (Sus scrofa) in The Netherlands: analyses of serological results and the first identification of Brucella suis biovar 2

2020 ◽  
Vol 10 (1) ◽  
pp. 1794668
Author(s):  
Peter van Tulden ◽  
Jose L. Gonzales ◽  
Michiel Kroese ◽  
Marc Engelsma ◽  
Frido de Zwart ◽  
...  
Keyword(s):  
The Netherlands ◽  
Wild Boar ◽  
Sus Scrofa ◽  
Brucella Suis

Quantification and genetic diversity of Hepatitis E virus in wild boar (Sus scrofa) hunted for domestic consumption in Central Italy

2019 ◽  
Vol 82 ◽  
pp. 194-201 ◽  
Author(s):  
Simona Di Pasquale ◽  
Paola De Santis ◽  
Giuseppina La Rosa ◽  
Kevin Di Domenico ◽  
Marcello Iaconelli ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Wild Boar ◽  
Sus Scrofa ◽  
Hepatitis E Virus ◽  
Central Italy ◽  
Hepatitis E ◽  
Domestic Consumption

Detection of Echinococcus granulosus G3 in a Wild Boar (Sus scrofa) in Central Italy using PCR and Sequencing

2017 ◽  
Vol 53 (2) ◽  
pp. 399-401 ◽  
Author(s):  
Antonella Di Paolo ◽  
Toni Piseddu ◽  
Martina Sebastianelli ◽  
Elisabetta Manuali ◽  
Sara Corneli ◽  
...  
Keyword(s):  
Wild Boar ◽  
Echinococcus Granulosus ◽  
Sus Scrofa ◽  
Central Italy

Lung parasites of the genus Metastrongylus Molin, 1861 (Nematoda: Metastrongilidae) in wild boar (Sus scrofa L., 1758) in Central-Italy: An eco-epidemiological study

2016 ◽  
Vol 217 ◽  
pp. 45-52 ◽  
Author(s):  
Giovanni Poglayen ◽  
Barbara Marchesi ◽  
Giulia Dall'Oglio ◽  
Giulia Barlozzari ◽  
Roberta Galuppi ◽  
...  
Keyword(s):  
Wild Boar ◽  
Epidemiological Study ◽  
Sus Scrofa ◽  
Central Italy
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