scholarly journals Seroprevalence and Molecular Identification of Brucella spp. in Camels in Egypt

2020 ◽  
Vol 8 (7) ◽  
pp. 1035 ◽  
Author(s):  
Aman Ullah Khan ◽  
Ashraf E. Sayour ◽  
Falk Melzer ◽  
Sherif Abdel Ghafar Elsayed El-Soally ◽  
Mandy C. Elschner ◽  
...  
Keyword(s):  
Complement Fixation Test ◽  
Indirect Elisa ◽  
Complement Fixation ◽  
Brucella Melitensis ◽  
Control Strategies ◽  
Effective Control ◽  
Brucella Species ◽  
Serum Samples ◽  
Brucella Suis ◽  
The Rose

Brucellosis is one of the most important worldwide zoonoses of many countries including Egypt. Camel brucellosis has not gained much attention in Egypt yet. This study is focused on the three governorates with the highest camel populations and the largest camel markets in the country to determine the disease seroprevalence and identify the Brucella species in local camel holdings. In total, 381 serum samples were collected from male and female camels from Giza, Aswan, and Al-Bahr Al-Ahmar (the Red Sea) governorates. Samples were serologically examined using the Rose–Bengal plate test (RBPT), indirect ELISA (i-ELISA), competitive ELISA (c-ELISA) and complement fixation test (CFT). Brucella antibodies were detected in 59 (15.5%), 87 (22.8%), 77 (20.2%) and 118 (31.0%) of sera by RBPT, i-ELISA, c-ELISA and CFT, respectively. Using real-time PCR, Brucella DNA was amplified in 32 (8.4%) seropositive samples including Brucella abortus (25/32), Brucella suis (5/32) and Brucella melitensis (2/32), defining a complex epidemiological status. To the best of our knowledge, this is the first study reporting Brucella suis DNA in camel serum. The risk-associated factors including age, sex, breed and geographical distribution were statistically analyzed, showing non-significant association with seroprevalence. The results of this study will raise awareness for camel brucellosis and help develop effective control strategies.

Brucella suis infection in domestic pigs in Sardinia (Italy)

2014 ◽  
Vol 143 (10) ◽  
pp. 2170-2177 ◽  
Author(s):  
C. PILO ◽  
M. T. TEDDE ◽  
G. ORRÙ ◽  
G. ADDIS ◽  
M. LICIARDI
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Bacteriological Examination ◽  
Chain Reaction ◽  
Serum Samples ◽  
Domestic Pigs ◽  
Brucella Suis ◽  
Commercial Farms ◽  
Polymerase Chain ◽  
The Rose

SUMMARYDuring a 4-year (2007–2010) survey, the presence of Brucella suis infection in domestic pigs in Sardinia was investigated. Serum samples were collected from breeding pigs located on 108 commercial farms with documented reproductive problems and analysed using the Rose Bengal (RBT) and complement fixation (CFT) tests for screening and confirmation of Brucella, respectively. Of the 1251 serum samples analysed by RBT, 406 sera, originating from 36 farms, were positive for B. suis. CFT was positive in 292/748 sera analysed, confirming positivity in all 36 pig herds. Pigs with international complement fixation test units per ml (ICFTU/ml) values ⩾160 were slaughtered, and their organs collected for bacteriological examination and testing by polymerase chain reaction (PCR). Brucella spp. strains were isolated in culture from 13/502 organs analysed, and subsequently identified as B. suis biovar 2. PCR detected positivity to Brucella spp. in 19/285 organs analysed. These results confirm the presence and emergence of B. suis infection in domestic pigs in Sardinia.

scholarly journals Serological and Molecular Identification of Brucella spp. in Pigs from Cairo and Giza Governorates, Egypt

Pathogens ◽  
2019 ◽  
Vol 8 (4) ◽  
pp. 248 ◽  
Author(s):  
Khan ◽  
Melzer ◽  
El-Soally ◽  
Elschner ◽  
Mohamed ◽  
...  
Keyword(s):  
Real Time Pcr ◽  
Brucella Melitensis ◽  
Control Strategies ◽  
Farm Animals ◽  
Effective Control ◽  
Serum Samples ◽  
Blood Samples ◽  
Male And Female ◽  
Molecular Assays ◽  
Brucella Suis

Brucellosis is considered as endemic disease of animals and humans since thousands of years in Egypt. However, brucellosis in pigs has never been reported in Egypt. Thus, serological and molecular assays were applied to detect anti-Brucella antibodies and DNA in serum samples collected from pigs. In total 331 blood samples collected from male and female pigs at slaughterhouses of Cairo and Giza governorates were investigated using Brucella c- and i-ELISA and Brucella real-time PCR. Anti-Brucella antibodies were detected in 16 (4.83%) and 36 (10.8%) sera by i-ELISA and c-ELISA, respectively. Brucella DNA was detected in 10 (3.02%) seropositive samples and identified as Brucella melitensis (7/10) and Brucella suis (3/10). A higher prevelance was found in boars. This is the first study investigating pig brucellosis in Egypt. The results of this study will raise awareness for brucellosis in these farm animals and will help to develop effective control strategies.

scholarly journals Genital Brucella suis Biovar 2 Infection of Wild Boar (Sus scrofa) Hunted in Tuscany (Italy)

2021 ◽  
Vol 9 (3) ◽  
pp. 582
Author(s):  
Giovanni Cilia ◽  
Filippo Fratini ◽  
Barbara Turchi ◽  
Marta Angelini ◽  
Domenico Cerri ◽  
...  
Keyword(s):  
Lymph Nodes ◽  
Wild Boar ◽  
Sus Scrofa ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Central Italy ◽  
Brucella Species ◽  
Molecular Assays ◽  
Brucella Suis ◽  
Venereal Transmission

Brucellosis is a zoonosis caused by different Brucella species. Wild boar (Sus scrofa) could be infected by some species and represents an important reservoir, especially for B. suis biovar 2. This study aimed to investigate the prevalence of Brucella spp. by serological and molecular assays in wild boar hunted in Tuscany (Italy) during two hunting seasons. From 287 animals, sera, lymph nodes, livers, spleens, and reproductive system organs were collected. Within sera, 16 (5.74%) were positive to both rose bengal test (RBT) and complement fixation test (CFT), with titres ranging from 1:4 to 1:16 (corresponding to 20 and 80 ICFTU/mL, respectively). Brucella spp. DNA was detected in four lymph nodes (1.40%), five epididymides (1.74%), and one fetus pool (2.22%). All positive PCR samples belonged to Brucella suis biovar 2. The results of this investigation confirmed that wild boar represents a host for B.suis biovar. 2 and plays an important role in the epidemiology of brucellosis in central Italy. Additionally, epididymis localization confirms the possible venereal transmission.

scholarly journals The serological response of cattle to vaccines against brucellosis, as measured by the brucellosis radioimmunoassay and other tests

1982 ◽  
Vol 88 (1) ◽  
pp. 11-19 ◽  
Author(s):  
R. J. Chappel ◽  
J. Hayes ◽  
B. A. Rogerson ◽  
L. J. Shenfield
Keyword(s):  
Brucella Abortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Agglutination Test ◽  
Serological Response ◽  
Serum Samples ◽  
Plate Test ◽  
The Rose ◽  
Serum Agglutination ◽  
Haemolysis Test

SummarySerum samples were obtained from 281 heifers vaccinated with Brucella abortus strain 19, and from 50 heifers that had received two injections of killed B. abortus strain 45/20 adjuvant (K45/20A) vaccine. The serological response measured by the brucellosis radioimmunoassay (RIA) was compared with responses measured by other tests.The serological responses of cattle during the first weeks after strain 19 vaccination were found to give little guide to the frequency of persistent reactions.In the case of strain 19, persistent reactions were considered to be those occurring 12 or more months after vaccination. In heifers vaccinated at the recommended age, small numbers of persistent reactions were given by the RIA (four in 374 sera), the complement fixation test using warm fixation (CFTW) (six in 383) and cold fixation (one in 185), the serum agglutination test (two in 222) and the indirect haemolysis test (IHLT) (two in 369). The Rose Bengal plate test gave 74 persistent reactions in 374 sera.Five of the 50 heifers gave particularly prolonged responses to K45/20A vaccine. In these animals the RIA and IHLT remained positive for longer than the CFTW.

Rapid detection of Chlamydia/Chlamydophila group in samples collected from swine herds with and without reproductive disorders

2014 ◽  
Vol 17 (2) ◽  
pp. 367-369 ◽  
Author(s):  
K. Rypula ◽  
A. Kumala ◽  
P. Lis ◽  
K. Niemczuk ◽  
K. Płoneczka-Janeczko ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Reproductive Disorders ◽  
Serum Samples ◽  
Swine Herds

Abstract The study was carried out in seven reproductive herds of pigs. In three of them reproductive disorders were observed. Three herds consisted of 10-50 and four consisted of 120-500 adult sows and they were called small and medium, respectively. Fifty-seven adult sows were randomly selected from herds. Serum samples were tested using the complement fixation test and swabs from both eyes and from the vaginal vestibule were examined using real-time PCR. All serum samples were negative. Infected sows were present in each of the study herds. In total, there were 28 positive samples (53%, 28/48) in real-time PCR in sows with reproductive disorders and 35 (53%, 35/66) in sows selected from herds without problems in reproduction. One isolate proved to be Chlamydophila pecorum, whereas all the remaining were Chamydia suis

scholarly journals Brucella abortus RB51 and Hot Saline Extract from Brucella ovis as Antigens in a Complement Fixation Test Used To Detect Sheep Vaccinated with Brucella abortus RB51

2001 ◽  
Vol 8 (1) ◽  
pp. 119-122 ◽  
Author(s):  
Rosanna Adone ◽  
Franco Ciuchini
Keyword(s):  
Immune Responses ◽  
Brucella Abortus ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Infected Sheep ◽  
Fixation Test ◽  
Anticomplementary Activity ◽  
Serum Samples ◽  
Saline Extract ◽  
Brucella Ovis

ABSTRACT The efficacy of Brucella abortus RB51 and hot saline extract (HSE) from Brucella ovis as antigens in complement fixation (CF) tests was comparatively evaluated in detecting immune responses of sheep vaccinated with B. abortus strain RB51. For this study, four 5-month-old sheep were vaccinated subcutaneously with 5 × 109 CFU of RB51, and two sheep received saline. Serum samples collected at different times after vaccination were tested for the presence of antibodies to RB51 by a CF test with RB51 as antigen, previously deprived of anticomplementary activity, and with HSE antigen, which already used as the official antigen to detectB. ovis-infected sheep. The results showed that vaccinated sheep developed antibodies which reacted weakly against HSE antigen and these antibodies were detectable for 30 days after vaccination. However, antibodies to RB51 could be detected for a longer period after vaccination by using homologous RB51 antigen in CF tests. In fact, high titers were still present at 110 days postvaccination with RB51 antigen. Sera from sheep naturally infected with B. ovisalso reacted to RB51 but gave lower titers than those detected by HSE antigen. As expected, all sera from RB51-vaccinated sheep remained negative when tested with standard S-type Brucella standard antigens.

scholarly journals A quantitative comparison of the sensitivity of serological tests for bovine brucellosis to different antibody classes

1976 ◽  
Vol 76 (2) ◽  
pp. 287-298 ◽  
Author(s):  
G. S. Allan ◽  
R. J. Chappel ◽  
P. Williamson ◽  
D. J. McNaught
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Quantitative Comparison ◽  
Agglutination Test ◽  
Bovine Brucellosis ◽  
Serological Tests ◽  
Plate Test ◽  
Specific Antibodies ◽  
The Rose ◽  
Serum Agglutination

SUMMARYBrucella-specific antibodies of different immunoglobulin classes were quantitatively evaluated with respect to their efficiency in serological tests for bovine brucellosis.IgM reacted more efficiently than IgG1and IgG2in both the Rose Bengal plate test and serum agglutination test. The complement fixation test was found to be slightly more sensitive to IgM than to IgG1and did not react to IgG2.IgM was, however, partly inactivated when heated at 60°C. in the presence of serum.

scholarly journals Development of Immunochromatographic Assay for the Rapid Detection of Mycoplasma capricolum subsp. capripneumoniae Antibodies

2022 ◽  
Vol 12 ◽  
Author(s):  
Zhen Zhu ◽  
Guanggang Qu ◽  
Changjiang Wang ◽  
Lei Wang ◽  
Jige Du ◽  
...  
Keyword(s):  
Immunoglobulin G ◽  
Colloidal Gold ◽  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Immunochromatographic Assay ◽  
Economic Losses ◽  
Serum Samples ◽  
Mycoplasma Capricolum ◽  
Prokaryotic Expression System

Mycoplasma capricolum subsp. capripneumoniae (Mccp) is the cause of contagious caprine pleuropneumonia (CCPP), which is a highly significant respiratory disease in goats leading to significant economic losses in Africa and Asia. Currently available procedures for the diagnosis of CCPP have some limitations in sensitivity, specificity, operation time, requirement of sophisticated equipment or skilled personnel, and cost. In this study, we developed a rapid, sensitive, and specific colloidal gold-based immunochromatographic assay (GICA) strip for the efficient on-site detection of antibodies against Mccp in the serum within 10 min. For the preparation of this colloidal GICA strip, recombinant P20 protein, the membrane protein of Mccp, was expressed by Escherichia coli prokaryotic expression system after purification was used as the binding antigen in the test. The rabbit anti-goat immunoglobulin G labeled with the colloidal gold was used as the detection probe, whereas the goat anti-rabbit immunoglobulin G was coated on the nitrocellulose membrane as the control line. The concentration of the coating antibody was optimized, and the effectiveness of this colloidal GICA strip was evaluated. Our results proved that the detection limit of the test strip was up to 1:64 dilutions for the Mccp antibody-positive serum samples with no cross-reactivity with other pathogens commonly infecting small ruminants,including goat pox virus, peste des petits ruminants virus, foot-and-mouth disease virus type A, or other mycoplasmas. Moreover, the colloidal GICA strip was more sensitive and specific than the indirect hemagglutination assay for the detection of Mccp antibodies. The 106 clinical serum samples were detected by the colloidal GICA strip compared with the complement fixation test, demonstrating an 87.74% concordance with the complement fixation test. This novel colloidal GICA strip would be an effective tool for the cost-effective and rapid diagnosis of CCPP in the field.

scholarly journals Comparison of radioimmunoassay with the complement fixation test and the indirect haemolysis test in the field diagnosis of bovine brucellosis

1983 ◽  
Vol 90 (1) ◽  
pp. 67-70 ◽  
Author(s):  
R. J. Chappel ◽  
J. Hayes
Keyword(s):  
Complement Fixation Test ◽  
Complement Fixation ◽  
Fixation Test ◽  
Supporting Evidence ◽  
Bovine Brucellosis ◽  
Eradication Programme ◽  
Female Cattle ◽  
The Rose ◽  
Supplementary Test ◽  
Haemolysis Test

SUMMARYSera were collected from female cattle in 118 commercial herds being subjected to a programme to eradicate brucellosis by test and slaughter, in an area in which vaccination of heifer calves with Brucella abortus strain 19 was compulsory. Of 4583 sera positive by the Rose Bengal plate test, the brucellosis radioimmunoassay was positive for 1524, the complement fixation test for 1363 and the indirect haemolysis test for 1141. These figures, and supporting evidence from the eradication programme, suggest that the radioimmunoassay may be a useful supplementary test in problem herds.

scholarly journals Field evaluation of an iELISA and CF test for detection of IgG antibodies against Chlamydophila abortus in goats, sheep and rams

2012 ◽  
Vol 47 (No. 7) ◽  
pp. 195-198 ◽  
Author(s):  
M. Trávníček ◽  
D. Kováčová ◽  
Bhide MR ◽  
P. Zubrický ◽  
L. Čisláková
Keyword(s):  
Complement Fixation Test ◽  
Indirect Elisa ◽  
Complement Fixation ◽  
Field Evaluation ◽  
Fixation Test ◽  
Igg Antibodies ◽  
Herd Level ◽  
Chlamydophila Abortus ◽  
Moderate Agreement ◽  
Sera Samples

Blood sera samples from 99 clinically healthy goats, 230 sheep and 171 rams were investigated by CF test and indirect ELISA. In case of goats, 3.03% seroprevalence was detected, in sheep it was 3.04%, whereas, in case of rams seroprevalence was 0% by using complement fixation test. Using iELISA in the same groups the seroprevalences observed were, 24.24% in goats, 11.30% in sheep and 5.30% in rams. Indirect ELISA was found to be comparatively more sensitive than CF test in all three groups of animals for detection of IgG antibodies against Chlamydophila abortus. The iELISA used in this study can be used for screening at herd level like CF test, as there is moderate agreement (Kappa – 0.426) between these two tests.

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