scholarly journals One Pathway, Two Cyclic Non-ribosomal Pentapeptides: Heterologous Expression of BE-18257 Antibiotics and Pentaminomycins from Streptomyces cacaoi CA-170360

2021 ◽  
Vol 9 (1) ◽  
pp. 135
Author(s):  
Fernando Román-Hurtado ◽  
Marina Sánchez-Hidalgo ◽  
Jesús Martín ◽  
Francisco Javier Ortiz-López ◽  
Daniel Carretero-Molina ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Gene Cluster ◽  
Binding Protein ◽  
Biosynthetic Gene Cluster ◽  
Protein Family ◽  
Biosynthetic Gene ◽  
Penicillin Binding Protein ◽  
Peptide Synthetases

The strain Streptomyces cacaoi CA-170360 produces the cyclic pentapeptides pentaminomycins A–H and BE-18257 A–C, two families of cyclopeptides synthesized by two non-ribosomal peptide synthetases encoded in tandem within the same biosynthetic gene cluster. In this work, we have cloned and confirmed the heterologous expression of this biosynthetic gene cluster, demonstrating that each of the non-ribosomal peptide synthetases present in the cluster is involved in the biosynthesis of each group of cyclopeptides. In addition, we discuss the involvement of a stand-alone enzyme belonging to the Penicillin Binding Protein family in the release and macrocyclization of the peptides.

scholarly journals One pathway, two cyclic pentapeptides: heterologous expression of BE-18257 A-C and pentaminomycins A-E from Streptomyces cacaoi CA-170360

2020 ◽  
Author(s):  
Fernando Román-Hurtado ◽  
Marina Sánchez-Hidalgo ◽  
Jesús Martín ◽  
Francisco Javier Ortiz-López ◽  
Daniel Carretero-Molina ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Gene Cluster ◽  
Binding Protein ◽  
Biosynthetic Gene Cluster ◽  
Protein Family ◽  
Biosynthetic Gene ◽  
Penicillin Binding Protein ◽  
Nonribosomal Peptide ◽  
Nonribosomal Peptide Synthetases ◽  
Peptide Synthetases

1.AbstractThe strain Streptomyces cacaoi CA-170360 produces the cyclic pentapeptides pentaminomycins A-E and BE-18257 A-C, two families of cyclopeptides synthesized by two nonribosomal peptide synthetases encoded in tandem within the same biosynthetic gene cluster. In this work, we have cloned and confirmed the heterologous expression of this biosynthetic gene cluster, demonstrating that each of the nonribosomal peptide synthetases present in the cluster is involved in the biosynthesis of each group of cyclopeptides. In addition, we discuss the involvement of a stand-alone enzyme belonging to the Penicillin Binding Protein family in the release and macrocyclization of the peptides.

Direct Cloning, Genetic Engineering, and Heterologous Expression of the Syringolin Biosynthetic Gene Cluster inE. colithrough Red/ET Recombineering

ChemBioChem ◽  
2012 ◽  
Vol 13 (13) ◽  
pp. 1946-1952 ◽  
Author(s):  
Xiaoying Bian ◽  
Fan Huang ◽  
Francis A. Stewart ◽  
Liqiu Xia ◽  
Youming Zhang ◽  
...  
Keyword(s):  
Genetic Engineering ◽  
Heterologous Expression ◽  
Gene Cluster ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene ◽  
Direct Cloning

Identification of the kinanthraquinone biosynthetic gene cluster by expression of an atypical response regulator

2021 ◽  
Vol 85 (3) ◽  
pp. 714-721
Author(s):  
Risa Takao ◽  
Katsuyuki Sakai ◽  
Hiroyuki Koshino ◽  
Hiroyuki Osada ◽  
Shunji Takahashi
Keyword(s):  
Heterologous Expression ◽  
Gene Cluster ◽  
Secondary Metabolite ◽  
Response Regulator ◽  
Bac Library ◽  
Gene Clusters ◽  
Biosynthetic Gene Cluster ◽  
Gene Organization ◽  
Biosynthetic Gene ◽  
Streptomyces Sp

ABSTRACT Recent advances in genome sequencing have revealed a variety of secondary metabolite biosynthetic gene clusters in actinomycetes. Understanding the biosynthetic mechanism controlling secondary metabolite production is important for utilizing these gene clusters. In this study, we focused on the kinanthraquinone biosynthetic gene cluster, which has not been identified yet in Streptomyces sp. SN-593. Based on chemical structure, 5 type II polyketide synthase gene clusters were listed from the genome sequence of Streptomyces sp. SN-593. Among them, a candidate gene cluster was selected by comparing the gene organization with grincamycin, which is synthesized through an intermediate similar to kinanthraquinone. We initially utilized a BAC library for subcloning the kiq gene cluster, performed heterologous expression in Streptomyces lividans TK23, and identified the production of kinanthraquinone and kinanthraquinone B. We also found that heterologous expression of kiqA, which belongs to the DNA-binding response regulator OmpR family, dramatically enhanced the production of kinanthraquinones.

scholarly journals Heterologous Expression of the Core Genes in the Complex Fusarubin Gene Cluster of Fusarium Solani

2020 ◽  
Vol 21 (20) ◽  
pp. 7601
Author(s):  
Tobias Bruun Pedersen ◽  
Mikkel Rank Nielsen ◽  
Sebastian Birkedal Kristensen ◽  
Eva Mie Lang Spedtsberg ◽  
Wafaa Yasmine ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Gene Cluster ◽  
Fusarium Solani ◽  
Biosynthetic Gene Cluster ◽  
Vector System ◽  
Biosynthetic Gene ◽  
The Core ◽  
Maximum Titer ◽  
Sequential Transformation ◽  
Core Genes

Through stepwise recreation of the biosynthetic gene cluster containing PKS3 from Fusarium solani, it was possible to produce the core scaffold compound of bostrycoidin, a red aza-anthraquinone pigment in Saccharomyces cerevisiae. This was achieved through sequential transformation associated recombination (TAR) cloning of FvPPT, fsr1, fsr2, and fsr3 into the pESC-vector system, utilizing the inducible bidirectional galactose promoter for heterologous expression in S. cerevisiae. The production of the core metabolite bostrycoidin was investigated through triplicate growth cultures for 1–4 days, where the maximum titer of bostrycoidin was achieved after 2 days of induction, yielding 2.2 mg/L.

Cloning and Heterologous Expression of the Penicillin Biosynthetic Gene Cluster from Penicillium chrysogenum

1990 ◽  
Vol 8 (1) ◽  
pp. 39-41 ◽  
Author(s):  
David J. Smith ◽  
Martin K. R. Burnham ◽  
Jeffrey Edwards ◽  
Alison J. Earl ◽  
Geoffrey Turner
Keyword(s):  
Heterologous Expression ◽  
Gene Cluster ◽  
Penicillium Chrysogenum ◽  
Biosynthetic Gene Cluster ◽  
Biosynthetic Gene
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