scholarly journals Greater Biofilm Formation and Increased Biodegradation of Polyethylene Film by a Microbial Consortium of Arthrobacter sp. and Streptomyces sp.

2020 ◽  
Vol 8 (12) ◽  
pp. 1979
Author(s):  
Ya-Nan Han ◽  
Min Wei ◽  
Fang Han ◽  
Chao Fang ◽  
Dong Wang ◽  
...  

The widespread use of polyethylene (PE) mulch films has led to a significant accumulation of plastic waste in agricultural soils. The biodegradation of plastic waste by microorganisms promises to provide a cost-effective and environmentally-friendly alternative for mitigating soil plastic pollution. A large number of microorganisms capable of degrading PE have been reported, but degradation may be further enhanced by the cooperative activity of multiple microbial species. Here, two novel strains of Arthrobacter sp. and Streptomyces sp. were isolated from agricultural soils and shown to grow with PE film as a sole carbon source. Arthrobacter sp. mainly grew in the suspension phase of the culture, and Streptomyces sp. formed substantial biofilms on the surface of the PE film, indicating that these strains were of different metabolic types and occupied different microenvironments with contrasting nutritional access. Individual strains were able to degrade the PE film to some extent in a 90-day inoculation experiment, as indicated by decreased hydrophobicity, increased carbonyl index and CO2 evolution, and the formation of biofilms on the film surface. However, a consortium of both strains had a much greater effect on these degradation properties. Together, these results provide new insights into the mechanisms of PE biodegradation by a microbial consortium composed of different types of microbes with possible metabolic complementarities.

This study deals with the development of a polyethylene terephthalate or PET plastic shredder for Xavier University in order to reduce the transportation cost of used plastic bottles to the designated dump site some 9 kilometers uphill. The development of this 2 horsepower plastic shredder is also useful for the reduction of the volume of plastic materials in the Material Recovery Facility of the municipality of Alubijid, Misamis Oriental. Xavier University has a collection of used plastic bottles of around 365 kg per month with an aggregate volume of 17.42 m3 and until the next garbage collection services arrive, the amount of plastic waste will occupy a considerable volume or space. This study focuses on the development and testing of the capacity of the developed plastic shredder. This study is inspired by the project of Mr. Dave Hakkens, called Precious Plastic, that aims to fight plastic pollution by developing machines that aid in recycling all sorts of plastic materials. The objective of this study is to analyze the cost of developing these machines in the Xavier University setting, their cost of operation, and effectiveness through minimizing the volume of plastic bottle. In this study, only polyethylene terephthalate (PET) plastic are being used. By consultation of local manufacturing firms in developing the machines through their specialized equipment, the authors have found that cost of development is high. However, a compromise between quality and precision has been decided to reduce the overall cost of the development of the machine. The authors were able to develop the proposed machines through the application of skills in machine workshop practices in the mechanical engineering laboratory. The XU plastic shredder has an average plastic volume reduction of 82.41%. With this volume reduction, the number of trips for the garbage truck collector is also reduced by 82.41% thereby saving delivery cost and manpower requirements. The cost of power consumption of the XU Plastic Shredder is only PhP36.52 per hour.


2021 ◽  
Vol 11 (1) ◽  
Author(s):  
R. Becerril-Arreola ◽  
R. E. Bucklin

AbstractPlastic pollution is a pressing issue because authorities struggle to contain and process the enormous amount of waste produced. We study the potential for reducing plastic waste by examining the efficiency with which different polyethylene terephthalate (PET) bottles deliver beverages. We find that 80% of the variation in bottle weight is explained by bottle capacity, 16% by product category, and 1% by brand. Bottle weight is quadratic and convex function of capacity, which implies that medium capacity bottles are most efficient at delivering consumable product. Local data on PET bottle sales and municipal waste recovery validate the findings. A 20% shift in consumption from smaller to larger bottles could reduce the production of PET waste by over 10,000 t annually in the U.S. alone.


2021 ◽  
Vol 22 (7) ◽  
pp. 3491
Author(s):  
Grażyna B. Dąbrowska ◽  
Zuzanna Garstecka ◽  
Ewa Olewnik-Kruszkowska ◽  
Grażyna Szczepańska ◽  
Maciej Ostrowski ◽  
...  

Plastic pollution is one of the crucial global challenges nowadays, and biodegradation is a promising approach to manage plastic waste in an environment-friendly and cost-effective way. In this study we identified the strain of fungus Trichoderma viride GZ1, which was characterized by particularly high pectinolytic activity. Using differential scanning calorimetry, Fourier-transform infrared spectroscopy techniques, and viscosity measurements we showed that three-month incubation of polylactide and polyethylene terephthalate in the presence of the fungus lead to significant changes of the surface of polylactide. Further, to gain insight into molecular mechanisms underneath the biodegradation process, western blot hybridization was used to show that in the presence of poly(ethylene terephthalate) (PET) in laboratory conditions the fungus produced hydrophobin proteins. The mycelium adhered to the plastic surface, which was confirmed by scanning electron microscopy, possibly due to the presence of hydrophobins. Further, using atomic force microscopy we demonstrated for the first time the formation of hydrophobin film on the surface of aliphatic polylactide (PLA) and PET by T. viride GZ1. This is the first stage of research that will be continued under environmental conditions, potentially leading to a practical application.


RSC Advances ◽  
2015 ◽  
Vol 5 (120) ◽  
pp. 99228-99239 ◽  
Author(s):  
Priyanka A. Bedekar ◽  
Siddheshwar D. Kshirsagar ◽  
Avinash R. Gholave ◽  
Sanjay P. Govindwar

Combinatorial adsorption–biodegradation treatment of textile wastewater provides a cost effective and ecofriendly alternative to conventional physicochemical treatment methods.


2021 ◽  
Vol 10 (1) ◽  
pp. 39
Author(s):  
Xinhua Qi ◽  
Wenlong Yan ◽  
Zhibei Cao ◽  
Mingzhu Ding ◽  
Yingjin Yuan

Polyethylene terephthalate (PET) is a widely used plastic that is polymerized by terephthalic acid (TPA) and ethylene glycol (EG). In recent years, PET biodegradation and bioconversion have become important in solving environmental plastic pollution. More and more PET hydrolases have been discovered and modified, which mainly act on and degrade the ester bond of PET. The monomers, TPA and EG, can be further utilized by microorganisms, entering the tricarboxylic acid cycle (TCA cycle) or being converted into high value chemicals, and finally realizing the biodegradation and bioconversion of PET. Based on synthetic biology and metabolic engineering strategies, this review summarizes the current advances in the modified PET hydrolases, engineered microbial chassis in degrading PET, bioconversion pathways of PET monomers, and artificial microbial consortia in PET biodegradation and bioconversion. Artificial microbial consortium provides novel ideas for the biodegradation and bioconversion of PET or other complex polymers. It is helpful to realize the one-step bioconversion of PET into high value chemicals.


2020 ◽  
Vol 200 ◽  
pp. 02014
Author(s):  
Bachtiar W Mutaqin ◽  
Muh Aris Marfai ◽  
Muhammad Helmi ◽  
Nurhadi Nurhadi ◽  
Muhammad Rizali Umarella ◽  
...  

Human pressure on the coastal and aquatic surrounding ecosystem in Indonesia, through plastic waste, is increasing, considering that 60 % of the approximately 250 million people live in the coastal areas. Plastic waste originating from human activities has become a massive problem in almost all the small island and coastal regions, especially in the eastern part of Indonesia. This condition is caused by poor waste management and a lack of public awareness in disposing of waste in its place, including in an area known as its marine biodiversities and marine tourism spots like Masohi in Central Maluku. Also, the composition of waste is dominated by plastic waste that cannot be decomposed in a short period, continue circulated on the ocean currents, and will be deposited in coastal areas. Furthermore, some plastic waste will break down into micro-plastics that pollute not only the environment but also marine biota, which are often consumed by humans. This situation profoundly affects the sustainability and function of aquaecosystem services in coastal areas. Therefore, a comprehensive policy and regulation, and interdisciplinary study for analysing vulnerable coastal ecosystem, and mitigating the potential risk of plastic pollution in Masohi, Central Maluku are essential to be conducted.


2020 ◽  
Vol 1 (2) ◽  
pp. 88 ◽  
Author(s):  
Filson M Sidjabat ◽  
Yunita Ismail ◽  
Evi Rismauli

Waste problems in Indonesia has reached a critical point that is contaminating many aspects in community. Poor waste management in land have an impact in water pollutions, rivers, and toward the ocean. This rivers and oceans pollution are become the spotlights for the world and the government. As a part of education community in West Java, President University has an important role to educati and find solutions relate with this issues, one of them is to implement Thidharma in the form of social empowerment in Jatireja Village. This environmental education activities was sharing knowledge about plastic waste treatment and management that can be conducted in household scale, and to encourage behavioral change and awareness among communities. Creative product of eco-brick was also implemented tas a part of waste utilization to make valueable product. Environmental education are needed to improve community awareness on plastic waste in Indonesia, especially in West Java.


2021 ◽  
Author(s):  
Jesus Gomez-Velez ◽  
Stefan Krause

<p>Global plastic pollution is affecting ecosystems and human health globally. Proposing solutions and coping strategies for this threat requires a clear understanding of the processes controlling the fate and transport of mismanaged plastics at multiple scales, going from watersheds to regions and even continents. River corridors are the primary conveyor and trap for mismanaged plastic produced within the landscape and eventually released to the ocean. New approaches that apply technological sensing innovations for monitoring plastic waste in aquatic environments can improve observations and plastic waste datasets globally. However, our understanding of when, where, and how to target monitoring is limited, reducing the benefit gained. There is therefore a critical demand for predictions of hotspots (as well as hot moments) of plastic accumulation along river networks globally, in order to optimize observational capacity.     </p><p>Here, we present a new global flow and transport model for plastic waste in riverine environments. Our model predicts that only a small fraction (roughly 2.5%) of the global mismanaged plastic that entered rivers since the 1950s has been delivered to the ocean by 2020, with an overwhelming majority sequestered in freshwater ecosystems. Furthermore, we predict the patterns of mismanaged plastic accumulation and its residence time depend on (i) the topology and geometry of the river network, (ii) the relative location of plastic sources, and (ii) the relative location and trapping efficiency of flow regulation structures, primarily large dams. Our results highlight the role of rivers as major sinks for plastic waste and the need for targeted remedial strategies that consider the structure of the river network and anthropogenic regulation when proposing intervention measures and sampling efforts.</p>


Environments ◽  
2020 ◽  
Vol 7 (5) ◽  
pp. 38 ◽  
Author(s):  
Jessica Stubenrauch ◽  
Felix Ekardt

Plastic pollution in soils pose a major threat to soil health and soil fertility that are directly linked to food security and human health. In contrast to marine plastic pollution, this ubiquitous problem is thus far scientifically poorly understood and policy approaches that tackle plastic pollution in soils comprehensively do not exist. In this article, we apply a qualitative governance analysis to assess the effectiveness of existing policy instruments to avoid harmful plastic pollution in (agricultural) soils against the background of international environmental agreements. In particular, environmental and fertiliser legislation relevant to soil protection in the European Union and in Germany are assessed. Regulatory weaknesses and gaps of the respective legislation are identified, and proposals for enhanced command-and-control provisions developed. However, the legal analysis furthermore shows that plastic pollution ecologically is also a problem of quantity, which is difficult to solve exclusively through command-and-control legislation. Instead, comprehensive quantity-control instruments to phase out fossil fuels (worldwide and in all sectors) as required by climate protection law can be effective approaches to tackle plastic pollution in environmental media like agricultural soils as well.


Energies ◽  
2020 ◽  
Vol 13 (8) ◽  
pp. 2040 ◽  
Author(s):  
Sultan Majed Al-Salem ◽  
Yang Yang ◽  
Jiawei Wang ◽  
Gary Anthony Leeke

The increasing global waste plastic pollution is urging people to take immediate actions on effective plastic recycling and processing. In this work, we report the results of processing reclaimed plastic wastes from unsanitary landfill site in Kuwait by using a bench scale continuous auger pyrolysis system. The plastic feedstock was characterised. After a simple thermal densification process, the material was fed to the pyrolysis system at 500 °C. The pyro-oil and wax products were collected and characterised. The process mass balance was developed on dry basis, and the yields of pyro-oil, light wax, heavy wax and gases were 5.5, 23.8, 69.4 and 1.3 wt%, respectively. The findings have indicated that the reclamation of plastic waste from landfill was feasible in terms of the product distribution and characteristics. Further liquid analysis confirmed that the liquid products contained fractions that are comparable to petrol and diesel fuels. The wax products are viable and have potential application as coating, covering and lubrication.


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