scholarly journals Application of Erythromycin and/or Raoultella sp. Strain MC3 Alters the Metabolic Activity of Soil Microbial Communities as Revealed by the Community Level Physiological Profiling Approach

2020 ◽  
Vol 8 (12) ◽  
pp. 1860
Author(s):  
Mariusz Cycoń ◽  
Anna Markowicz ◽  
Tomasz J. Wąsik ◽  
Zofia Piotrowska-Seget
Keyword(s):  
Microbial Community ◽  
Microbial Activity ◽  
Soil Microorganisms ◽  
Soil Microbial Communities ◽  
Resistance Index ◽  
Community Level ◽  
Carbon Substrate ◽  
Soil Microbial ◽  
Catabolic Activity ◽  
Community Level Physiological Profiling

Erythromycin (EM), a macrolide antibiotic, by influencing the biodiversity of microorganisms, might change the catabolic activity of the entire soil microbial community. Hence, the goal of this study was to determine the metabolic biodiversity in soil treated with EM (1 and 10 mg/kg soil) using the community-level physiological profiling (CLPP) method during a 90-day experiment. In addition, the effect of soil inoculation with antibiotic-resistant Raoultella sp. strain MC3 on CLPP was evaluated. The resistance and resilience concept as well as multifactorial analysis of data was exploited to interpret the outcomes obtained. EM negatively affected the metabolic microbial activity, as indicated by the values of the CLPP indices, i.e., microbial activity expressed as the average well-color development (AWCD), substrate richness (R), the Shannon–Wiener (H) and evenness (E) indices and the AWCD values for the six groups of carbon substrate present in EcoPlates until 15 days. The introduction of strain MC3 into soil increased the degradative activity of soil microorganisms in comparison with non-inoculated control. In contrast, at the consecutive sampling days, an increase in the values of the CLPP parameters was observed, especially for EM-10 + MC3-treated soil. Considering the average values of the resistance index for all of the measurement days, the resistance of the CLPP indices and the AWCD values for carbon substrate groups were categorized as follows: E > H > R > AWCD and polymers > amino acids > carbohydrates > miscellaneous > amines > carboxylic acids. The obtained results suggest a low level of resistance of soil microorganisms to EM and/or strain MC3 at the beginning of the exposure time, but the microbial community exhibited the ability to recover its initial decrease in catabolic activity over the experimental period. Despite the short-term effects, the balance of the soil ecosystem may be disturbed.

scholarly journals Community level physiological profiling of microorganisms in the rhizosphere of transgenic plants – A review

2021 ◽  
Vol 10 (2) ◽  
Author(s):  
Juraj Faragó ◽  
Natália Faragová
Keyword(s):  
Transgenic Plants ◽  
Microbial Communities ◽  
Genetically Modified ◽  
Soil Microbial Communities ◽  
Genetically Modified Crops ◽  
Community Level ◽  
Carbon Substrate ◽  
Soil Microbial Diversity ◽  
Soil Microbial ◽  
Community Level Physiological Profiling

Since 1996, when the first genetically modified seeds were planted in field conditions, the commercial growing of genetically modified crops increased to over 134 millions of hectares in 2009 worldwide. Along with the great potential of transgenic plants for future agriculture, considerable concerns on their biosafety have been raised, including their potential impact on soil microbial communities. This review briefly summarizes the important features of soil microorganisms for plant health and ecosystem stability, the numerous methods available for microbial ecologists to study soil microbial diversity, with emphasis on the method of community level physiological profiling (CLPP) based on carbon substrate utilization patterning, and finally the use of CLPP for assessing the effects of transgenic crops on soil microbial communities.

scholarly journals Functional Diversity of Soil Microbial Communities in Response to the Application of Cefuroxime and/or Antibiotic-Resistant Pseudomonas putida Strain MC1

2018 ◽  
Vol 10 (10) ◽  
pp. 3549 ◽  
Author(s):  
Kamila Orlewska ◽  
Anna Markowicz ◽  
Zofia Piotrowska-Seget ◽  
Joanna Smoleń-Dzirba ◽  
Mariusz Cycoń
Keyword(s):  
Amino Acids ◽  
Microbial Community ◽  
Pseudomonas Putida ◽  
Carboxylic Acids ◽  
Functional Diversity ◽  
Soil Microbial Community ◽  
Carbon Substrate ◽  
Antibiotic Resistant ◽  
Soil Microbial ◽  
Catabolic Activity

Cefuroxime (XM), the most commonly prescribed antibiotic from the cephalosporin group, may cause changes in the structure of the soil microbial community, and these changes may also be reflected in the alteration of its functionality. Therefore, due to the lack of studies on this topic, the scope of this study was to assess the functional diversity and catabolic activity of the microbial community in soil treated with XM (1 mg/kg and 10 mg/kg soil) using the community-level physiological profile (CLPP) approach during a 90-day experiment. In addition, the effect of antibiotic-resistant Pseudomonas putida strain MC1 (Ps) was also evaluated. The resistance/resilience concept and multifactorial analysis were used to interpret the data. The results showed that the introduction of XM and/or Ps into the soil caused changes in the catabolic activity and functional diversity of the microbial community. A decrease in the values of the CLPP indices (i.e., microbial activity expressed as the average well-color development (AWCD), substrate richness (R), the Shannon-Wiener (H) and evenness (E) indices and the AWCD values for the six carbon substrate groups) for the XM-treated soil was generally detected up to 30 days. In turn, at the same time, the activity measured in the Ps-inoculated soil was higher compared to the control soil. A stimulatory effect of XM at 10 mg/kg (XM10) and XM10+Ps on the utilization pattern of each substrate group was found at the following sampling times (days 60 and 90). The AWCD values for the utilization of amines, amino acids, carbohydrates, carboxylic acids, miscellaneous compounds and polymers for these treatments were found to be up to 2.3-, 3.1-, 2.3-, 13-, 3.4- and 3.3-fold higher compared to the values for the nontreated control, respectively. The resistance of the CLPP indices and the AWCD values for the carbon substrate groups were categorized as follows: E > H > R > AWCD and amino acids = carbohydrates > polymers > amines > miscellaneous > carboxylic acids, respectively. The results suggest a low initial resistance of the soil microbial community to XM and/or Ps, and despite the short-term negative effect, the balance of the soil ecosystem may be disturbed.

scholarly journals Impact of Maize Formulated Herbicides Mesotrione and S-Metolachlor, Applied Alone and in Mixture, on Soil Microbial Communities.

ISRN Ecology ◽  
2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Pierre Joly ◽  
Pascale Besse-Hoggan ◽  
Frédérique Bonnemoy ◽  
Isabelle Batisson ◽  
Jacques Bohatier ◽  
...  
Keyword(s):  
Microbial Community ◽  
Microbial Communities ◽  
Microbial Activity ◽  
Synergetic Effect ◽  
Soil Microbial Communities ◽  
Soil Microbial Activity ◽  
Soil Microbial ◽  
Lower Weight ◽  
Global Soil ◽  
The Impact

In order to reduce the amounts of pesticides used, and thereby their associated risks, new generations of less environmentally dangerous molecules with lower weight are currently being used in the mixtures sprayed on crops. Few studies have been made, however, to analyse their impact on the soil, and more particularly on the microorganisms living in the soil which maintain the essential functions of this ecosystem. By taking a microcosmic approach, we were able to assess the impact of the maize herbicides “cocktail” Mesotrione and S-metolachlor on global soil microbial activity, biomass, and structures, by using the formulated compounds, respectively, Callisto and Dual Gold (both registered brands of Syngenta). Our results highlighted a synergetic effect in “cocktail” microcosms resulting in an increase in the Mesotrione herbicide dissipation time and in an impact on the microbial community at onefold field rate equally to more than a single herbicide used at tenfold field rate.

scholarly journals Comparison of Drivers of Soil Microbial Communities Developed in Karst Ecosystems with Shallow and Deep Soil Depths

Agronomy ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 173
Author(s):  
Huiling Guan ◽  
Jiangwen Fan ◽  
Haiyan Zhang ◽  
Warwick Harris
Keyword(s):  
Microbial Community ◽  
Microbial Communities ◽  
Soil Depth ◽  
Phospholipid Fatty Acid ◽  
Soil Microbial Communities ◽  
Deep Soil ◽  
Soil System ◽  
Soil Microbial ◽  
Total Soil ◽  
Karst Ecosystems

Soil erosion is prevalent in karst areas, but few studies have compared the differences in the drivers for soil microbial communities among karst ecosystems with different soil depths, and most studies have focused on the local scale. To fill this research gap, we investigated the upper 20 cm soil layers of 10 shallow–soil depth (shallow–SDC, total soil depth less than 100 cm) and 11 deep–soil depth communities (deep–SDC, total soil depth more than 100 cm), covering a broad range of vegetation types, soils, and climates. The microbial community characteristics of both the shallow–SDC and deep–SDC soils were tested by phospholipid fatty acid (PLFAs) analysis, and the key drivers of the microbial communities were illustrated by forward selection and variance partitioning analysis. Our findings demonstrated that more abundant soil nutrients supported higher fungal PLFA in shallow–SDC than in deep–SDC (p < 0.05). Furthermore, stronger correlation between the microbial community and the plant–soil system was found in shallow–SDC: the pure plant effect explained the 43.2% of variance in microbial biomass and 57.8% of the variance in the ratio of Gram–positive bacteria to Gram–negative bacteria (G+/G−), and the ratio of fungi to total bacteria (F/B); the pure soil effect accounted for 68.6% variance in the microbial diversity. The ratio of microbial PLFA cyclopropyl to precursors (Cy/Pr) and the ratio of saturated PLFA to monounsaturated PLFA (S/M) as indicators of microbial stress were controlled by pH, but high pH was not conducive to microorganisms in this area. Meanwhile, Cy/Pr in all communities was >0.1, indicating that microorganisms were under environmental stress. Therefore, the further ecological restoration of degraded karst communities is needed to improve their microbial communities.

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