scholarly journals Functional Diversity of Soil Microbial Communities in Response to the Application of Cefuroxime and/or Antibiotic-Resistant Pseudomonas putida Strain MC1

2018 ◽  
Vol 10 (10) ◽  
pp. 3549 ◽  
Author(s):  
Kamila Orlewska ◽  
Anna Markowicz ◽  
Zofia Piotrowska-Seget ◽  
Joanna Smoleń-Dzirba ◽  
Mariusz Cycoń
Keyword(s):  
Amino Acids ◽  
Microbial Community ◽  
Pseudomonas Putida ◽  
Carboxylic Acids ◽  
Functional Diversity ◽  
Soil Microbial Community ◽  
Carbon Substrate ◽  
Antibiotic Resistant ◽  
Soil Microbial ◽  
Catabolic Activity

Cefuroxime (XM), the most commonly prescribed antibiotic from the cephalosporin group, may cause changes in the structure of the soil microbial community, and these changes may also be reflected in the alteration of its functionality. Therefore, due to the lack of studies on this topic, the scope of this study was to assess the functional diversity and catabolic activity of the microbial community in soil treated with XM (1 mg/kg and 10 mg/kg soil) using the community-level physiological profile (CLPP) approach during a 90-day experiment. In addition, the effect of antibiotic-resistant Pseudomonas putida strain MC1 (Ps) was also evaluated. The resistance/resilience concept and multifactorial analysis were used to interpret the data. The results showed that the introduction of XM and/or Ps into the soil caused changes in the catabolic activity and functional diversity of the microbial community. A decrease in the values of the CLPP indices (i.e., microbial activity expressed as the average well-color development (AWCD), substrate richness (R), the Shannon-Wiener (H) and evenness (E) indices and the AWCD values for the six carbon substrate groups) for the XM-treated soil was generally detected up to 30 days. In turn, at the same time, the activity measured in the Ps-inoculated soil was higher compared to the control soil. A stimulatory effect of XM at 10 mg/kg (XM10) and XM10+Ps on the utilization pattern of each substrate group was found at the following sampling times (days 60 and 90). The AWCD values for the utilization of amines, amino acids, carbohydrates, carboxylic acids, miscellaneous compounds and polymers for these treatments were found to be up to 2.3-, 3.1-, 2.3-, 13-, 3.4- and 3.3-fold higher compared to the values for the nontreated control, respectively. The resistance of the CLPP indices and the AWCD values for the carbon substrate groups were categorized as follows: E > H > R > AWCD and amino acids = carbohydrates > polymers > amines > miscellaneous > carboxylic acids, respectively. The results suggest a low initial resistance of the soil microbial community to XM and/or Ps, and despite the short-term negative effect, the balance of the soil ecosystem may be disturbed.

Soil Microbial Functional Diversity from Different Infection Grades of Banana Fusarium Wilt (Fusarium oxysporum f. sp. Cubense)

2013 ◽  
Vol 295-298 ◽  
pp. 2274-2280 ◽  
Author(s):  
Xiao Deng ◽  
Qin Fen Li ◽  
Xian Wen Hou ◽  
Chun Yuan Wu
Keyword(s):  
Microbial Community ◽  
Fusarium Oxysporum ◽  
Functional Diversity ◽  
Fusarium Wilt ◽  
Soil Microbial Community ◽  
Rhizosphere Soil ◽  
Principal Component ◽  
Soil Microbial ◽  
Metabolic Characteristics ◽  
Environmental Measures

Thirty rhizosphere and non-rhizosphere soil samples from different infection grades(0, I, III, V and VII) of three typical banana plots(Jianfeng, Shiyuetian, Chongpo) infected by banana fusarium wilt (Fusarium oxysporum f. sp. cubense) in Hainan province were collected to study the microbial community functional diversity applying Biolog-ECO microplates technology. The results are as follows: (1) Overall carbon source metabolic capacities of soil microbial community weaken with increasing of infection grades of banana fusarium wilt. (2) Richness indices, Simpson indices, Shannon indices and McIntosh indices of soil microbial community gradually decreased with increasing of infection grades of banana fusarium wilt. (3) Principal component analysis show that metabolic characteristics of soil microbial community significantly change between the healthy plants and diseased plants in the same banana plot. The results would provide information for explaining the pathogenesis of banana fusarium wilt and controlling its incidence by applying microbial ecology to regulate soil environmental measures.

Acetaminophen Concentrations Found in Recycled Wastewater alter Soil Microbial Community Structure and Functional Diversity

2021 ◽  
Author(s):  
Nathan K. McLain ◽  
Melissa Y. Gomez ◽  
Emma W. Gachomo
Keyword(s):  
Microbial Community ◽  
Carboxylic Acids ◽  
Soil Microbial Community ◽  
Pharmaceutical Products ◽  
Soil Microbiome ◽  
Agricultural Field ◽  
Soil Microbial Community Structure ◽  
Soil Microbial ◽  
Greenhouse Study ◽  
Biolog Ecoplate

Abstract The practice of using recycled wastewater (RWW) (aka reclaimed water) has been adopted to address the growing demand for clean water and has been largely successful. However, chemicals of emerging concern (CECs) including pharmaceutical products such as acetaminophen (APAP) remain in the water even after additional cleaning. When recycled waste water is used to irrigate crops or landscapes these chemicals can then enter these and adjacent environments. APAP is one such contaminant and has the potential to disrupt the soil microbiome associated with agricultural crops. We evaluated this possibility using a greenhouse study to mimic agricultural field conditions, and applied treatments of APAP concentrations found within the ranges observed for RWW effluents. Using Illumina sequencing based approaches we observed that APAP was able to cause shifts in the microbial community and positively select for bacteria that are capable of metabolizing the breakdown products of APAP such as glycosides and carboxylic acids. Community-level physiological profiles of carbon metabolism were evaluated using Biolog EcoPlate as a proxy for community functions. The biolog plates indicated that the metabolism of amino acids, carbohydrates, carboxylic acids, phenolics, and polymers was significantly higher in the presence of APAP. Together our observations indicate that the soil microbial community and functions were altered by APAP and demonstrates the need to change current RWW policies to address the presence of these chemicals.

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