scholarly journals Quorum Quenching in a Novel Acinetobacter sp. XN-10 Bacterial Strain against Pectobacterium carotovorum subsp. carotovorum

2020 ◽  
Vol 8 (8) ◽  
pp. 1100 ◽  
Author(s):  
Wenping Zhang ◽  
Qingqing Luo ◽  
Yiyin Zhang ◽  
Xinghui Fan ◽  
Tian Ye ◽  
...  
Keyword(s):  
Bacterial Pathogens ◽  
Quorum Quenching ◽  
Homoserine Lactone ◽  
Gas Chromatography Mass Spectrometry ◽  
Pectobacterium Carotovorum ◽  
Degrading Bacterium ◽  
Family Analysis ◽  
Carotovorum Subsp ◽  
Acinetobacter Sp ◽  
Tissue Maceration

Quorum sensing (QS) is a cell density-dependent mechanism that regulates the expression of specific genes in microbial cells. Quorum quenching (QQ) is a promising strategy for attenuating pathogenicity by interfering with the QS system of pathogens. N-Acyl-homoserine lactones (AHLs) act as signaling molecules in many Gram-negative bacterial pathogens and have received wide attention. In this study, a novel, efficient AHL-degrading bacterium, Acinetobacter sp. strain XN-10, was isolated from agricultural contaminated soil and evaluated for its degradation efficiency and potential use against QS-mediated pathogens. Strain XN-10 could effectively degrade N-(3-oxohexanoyl)-L-homoserine lactone (OHHL), N-hexanoyl-L-homoserine lactone (C6HSL), N-(3-oxododecanoyl)-L-homoserine lactone (3OC12HSL), and N-(3-oxooctanoyl)-L-homoserine lactone (3OC8HSL), which all belong to the AHL family. Analysis of AHL metabolic products by gas chromatography–mass spectrometry (GC-MS) led to the identification of N-cyclohexyl-propanamide, and pentanoic acid, 4-methyl, methyl ester as the main intermediate metabolites, revealing that AHL could be degraded by hydrolysis and dehydroxylation. All intermediates were transitory and faded away without any non-cleavable metabolites at the end of the experiment. Furthermore, strain XN-10 significantly attenuated the pathogenicity of Pectobacterium carotovorum subsp. carotovorum (Pcc) to suppress tissue maceration in carrots, potatoes, and Chinese cabbage. Taken together, our results shed light on the QQ mechanism of a novel AHL-degrading bacterial isolate, and they provide useful information which show potential for biocontrol of infectious diseases caused by AHL-dependent bacterial pathogens.

scholarly journals Characterization of a Novel Quorum-Quenching Bacterial Strain, Burkholderia anthina HN-8, and Its Biocontrol Potential against Black Rot Disease Caused by Xanthomonas campestris pv. campestris

2020 ◽  
Vol 8 (10) ◽  
pp. 1485
Author(s):  
Tian Ye ◽  
Wenping Zhang ◽  
Zhixuan Feng ◽  
Xinghui Fan ◽  
Xudan Xu ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Xanthomonas Campestris ◽  
Bacterial Pathogens ◽  
Quorum Quenching ◽  
Gas Chromatography Mass Spectrometry ◽  
Black Rot ◽  
Degrading Bacterium ◽  
Diffusible Signal Factor ◽  
Rot Disease ◽  
Xanthomonas Campestris Pv Campestris

Diffusible signal factor (DSF) is a type of cis unsaturated fatty acid, with a chemical structure of 11-methyl-2-dodecylene acid. DSF is widely conserved in a variety of Gram-negative bacterial pathogens and is involved in the regulation of pathogenic virulence. Quorum quenching (QQ) is a promising strategy for preventing and controlling quorum sensing (QS)-mediated bacterial infections by interfering with the QS system of pathogens. In this study, a novel DSF-degrading bacterium, Burkholderia anthina strain HN-8, was isolated and characterized for its degradation ability and potential biocontrol of black rot disease caused by Xanthomonas campestris pv. campestris (Xcc). The HN-8 strain exhibited superb DSF degradation activity and completely degraded 2 mM DSF within 48 h. In addition, we present the first evidence of bacterium having a metabolic pathway for the complete degradation and metabolism of DSF. Analysis of DSF metabolic products by gas chromatography–mass spectrometry led to the identification of dodecanal as the main intermediate product, revealing that DSF could be degraded via oxidation–reduction. Furthermore, application of strain HN-8 as a potent biocontrol agent was able to significantly reduce the severity of black rot disease in radishes and Chinese cabbage. Taken together, these results shed light on the QQ mechanisms of DSF, and they provide useful information showing the potential for the biocontrol of infectious diseases caused by DSF-dependent bacterial pathogens.

Screening of quorum-quenching bacteria associated with rhizosphere as biocontrol agents of Pectobacterium carotovorum subsp. carotovorum

2020 ◽  
Vol 53 (11-12) ◽  
pp. 509-523 ◽  
Author(s):  
Fatemeh Alinejad ◽  
Fatemeh Shahryari ◽  
Omid Eini ◽  
Fatemeh Sarafraz-Niko ◽  
Ahmad Shekari ◽  
...  
Keyword(s):  
Quorum Quenching ◽  
Biocontrol Agents ◽  
Pectobacterium Carotovorum ◽  
Carotovorum Subsp

scholarly journals Antagonistic activity of endophytic actinobacteria from native potatoes (Solanum tuberosum subsp. tuberosum L.) against Pectobacterium carotovorum subsp. carotovorum and Pectobacterium atrosepticum

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Natalia Padilla-Gálvez ◽  
Paola Luengo-Uribe ◽  
Sandra Mancilla ◽  
Amandine Maurin ◽  
Claudia Torres ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Plant Root ◽  
Soft Rot ◽  
Quorum Quenching ◽  
Antagonistic Activity ◽  
Pectobacterium Carotovorum ◽  
Double Labeling ◽  
Pectobacterium Atrosepticum ◽  
Tissue Maceration ◽  
Endophytic Actinobacteria

Abstract Background The native potatoes (Solanum tuberosum subsp. tuberosum L.) grown in Chile (Chiloé) represent a new, unexplored source of endophytes to find potential biological control agents for the prevention of bacterial diseases, like blackleg and soft rot, in potato crops. Result The objective of this study was the selection of endophytic actinobacteria from native potatoes for antagonistic activity against Pectobacterium carotovorum subsp. carotovorum and Pectobacterium atrosepticum, and their potential to suppress tissue maceration symptoms in potato tubers. This potential was determined through the quorum quenching activity using a Chromobacterium violaceaum ATCC 12472 Wild type (WT) bioassay and its colonization behavior of the potato plant root system (S. tuberosum) by means of the Double labeling of oligonucleotide probes for fluorescence in situ hybridization (DOPE-FISH) targeting technique. The results showed that although Streptomyces sp. TP199 and Streptomyces sp. A2R31 were able to inhibit the growth of the pathogens, only the Streptomyces sp. TP199 isolate inhibited Pectobacterium sp. growth and diminished tissue maceration in tubers (p ≤ 0.05). Streptomyces sp. TP199 had metal-dependent acyl homoserine lactones (AHL) quorum quenching activity in vitro and was able to colonize the root endosphere 10 days after inoculation. Conclusions We concluded that native potatoes from southern Chile possess endophyte actinobacteria that are potential agents for the disease management of soft rot and blackleg.

scholarly journals Exploration of the Quorum-Quenching Mechanism in Pseudomonas nitroreducens W-7 and Its Potential to Attenuate the Virulence of Dickeya zeae EC1

2021 ◽  
Vol 12 ◽  
Author(s):  
Wenping Zhang ◽  
Xinghui Fan ◽  
Jiayi Li ◽  
Tian Ye ◽  
Sandhya Mishra ◽  
...  
Keyword(s):  
Host Plants ◽  
Pathogenic Bacteria ◽  
Bacterial Pathogens ◽  
Disease Incidence ◽  
Quorum Quenching ◽  
Homoserine Lactone ◽  
Economic Losses ◽  
Highly Efficient ◽  
Pseudomonas Nitroreducens ◽  
Wide Range

Quorum quenching (QQ) is a novel, promising strategy that opens up a new perspective for controlling quorum-sensing (QS)-mediated bacterial pathogens. QQ is performed by interfering with population-sensing systems, such as by the inhibition of signal synthesis, catalysis of degrading enzymes, and modification of signals. In many Gram-negative pathogenic bacteria, a class of chemically conserved signaling molecules named N-acyl homoserine lactones (AHLs) have been widely studied. AHLs are involved in the modulation of virulence factors in various bacterial pathogens including Dickeya zeae. Dickeya zeae is the causal agent of plant-rot disease of bananas, rice, maize, potatoes, etc., causing enormous economic losses of crops. In this study, a highly efficient AHL-degrading bacterial strain W-7 was isolated from activated-sludge samples and identified as Pseudomonas nitroreducens. Strain W-7 revealed a superior ability to degrade N-(3-oxododecanoyl)-l-homoserine lactone (OdDHL) and completely degraded 0.2 mmol/L of OdDHL within 48 h. Gas chromatography-mass spectrometry (GC-MS) identified N-cyclohexyl-propanamide as the main intermediate metabolite during AHL biodegradation. A metabolic pathway for AHL in strain W-7 was proposed based on the chemical structure of AHL and intermediate products. In addition to the degradation of OdDHL, this strain was also found to be capable of degrading a wide range of AHLs including N-(3-oxohexanoyl)-l-homoserine lactone (OHHL), N-(3-oxooctanoyl)-l-homoserine lactone (OOHL), and N-hexanoyl-l-homoserine lactone (HHL). Moreover, the application of strain W-7 as a biocontrol agent could substantially attenuate the soft rot caused by D. zeae EC1 to suppress tissue maceration in various host plants. Similarly, the application of crude enzymes of strain W-7 significantly reduced the disease incidence and severity in host plants. These original findings unveil the biochemical aspects of a highly efficient AHL-degrading bacterial isolate and provide useful agents that exhibit great potential for the control of infectious diseases caused by AHL-dependent bacterial pathogens.

Detection and characterization of bacteria from the potato rhizosphere degradingN-acyl-homoserine lactone

2006 ◽  
Vol 52 (10) ◽  
pp. 1006-1015 ◽  
Author(s):  
S Jafra ◽  
J Przysowa ◽  
R Czajkowski ◽  
A Michta ◽  
P Garbeva ◽  
...  
Keyword(s):  
Quorum Sensing ◽  
Acid Methyl Ester ◽  
Soft Rot ◽  
Homoserine Lactone ◽  
Signal Molecules ◽  
Pectobacterium Carotovorum ◽  
In Planta ◽  
Lower Efficiency ◽  
Carotovorum Subsp ◽  
First Time

Quorum sensing plays a role in the regulation of soft rot diseases caused by the plant pathogenic bacterium Pectobacterium carotovorum subsp. carotovorum. The signal molecules involved in quorum sensing in P. carotovorum subsp. carotovorum belong to the group of N-acyl homoserine lactones (AHLs). In our study, we screened bacteria isolated from the potato rhizosphere for the ability to degrade AHLs produced by P. carotovorum subsp. carotovorum. Six isolates able to degrade AHLs were selected for further studies. According to 16S rDNA sequence analysis and fatty acid methyl ester profiling, the isolates belonged to the genera Ochrobactrum, Rhodococcus, Pseudomonas, Bacillus, and Delftia. For the genera Ochrobactrum and Delftia, for the first time AHL-degrading isolates were found. Data presented in this study revealed for the first time that Ochrobactrum sp. strain A44 showed the capacity to inactivate various synthetic AHL molecules; the substituted AHLs were inactivated with a lower efficiency than the unsubstituted AHLs. Compared with the other isolates, A44 was very effective in the degradation of AHLs produced by P. carotovorum subsp. carotovorum. It was verified by polymerase chain reaction, DNA–DNA hybridization, and a lactone ring reconstruction assay that Ochrobactrum sp. strain A44 did not possess AHL lactonase activity. AHL degradation in Ochrobactrum sp. strain A44 occurred intracellularly; it was not found in the culture supernatant. AHL-degrading activity of A44 was thermo sensitive. Experiments in planta revealed that Ochrobactrum sp. strain A44 significantly inhibited the maceration of potato tuber tissue. Since A44 did not produce antibiotics, the attenuation of the decay might be due to the quenching of quorum- sensing-regulated production of pectinolytic enzymes. The strain can potentially serve to control P. carotovorum subsp. carotovorum in potato.Key words: AHL degradation, Ochrobactrum sp., Pectobacterium carotovorum.

scholarly journals Cupriavidus sp. HN-2, a Novel Quorum Quenching Bacterial Isolate, is a Potent Biocontrol Agent Against Xanthomonas campestris pv. campestris

2019 ◽  
Vol 8 (1) ◽  
pp. 45 ◽  
Author(s):  
Tian Ye ◽  
Tian Zhou ◽  
Qiting Li ◽  
Xudan Xu ◽  
Xinghui Fan ◽  
...  
Keyword(s):  
Bacterial Infections ◽  
Xanthomonas Campestris ◽  
Biocontrol Agent ◽  
Bacterial Isolate ◽  
Bacterial Pathogens ◽  
Acid Methyl Ester ◽  
Quorum Quenching ◽  
Gas Chromatography Mass Spectrometry ◽  
Biochemical Basis ◽  
Xanthomonas Campestris Pv Campestris

Diffusible signal factor (DSF) represents a family of widely conserved quorum sensing (QS) signals involved in the regulation of virulence factor production in many Gram-negative bacterial pathogens. Quorum quenching, which disrupts QS either by degradation of QS signals or interference of signal generation or perception, is a promising strategy for prevention and control of QS-mediated bacterial infections. In this study, a novel DSF-degrading strain, HN-2, was isolated from contaminated soil and identified as Cupriavidus sp. The isolate exhibited superior DSF degradation activity and completely degraded 2 mmol·L–1 of DSF within 24 h. Analysis of the degradation products of DSF by gas chromatography–mass spectrometry led to the identification of trans-2-decenoic acid methyl ester as the main intermediate product, suggesting that DSF could be degraded by oxidation and hydroxylation. Moreover, this study presents for the first time, evidence that Cupriavidus sp. can reduce the black rot disease caused by Xanthomonas campestris pv. campestris (Xcc). Application of the HN-2 strain as a biocontrol agent could substantially reduce the disease severity. These findings reveal the biochemical basis of a highly efficient DSF-degrading bacterial isolate and present a useful agent for controlling infectious diseases caused by DSF-dependent bacterial pathogens.

scholarly journals Characterization of onion soft rot bacteria in Bangladesh

2017 ◽  
Vol 52 (3) ◽  
pp. 209-220
Author(s):  
MM Rahman ◽  
MAA Khan ◽  
IH Mian ◽  
AM Akanda ◽  
MZ Alam
Keyword(s):  
Bacterial Pathogens ◽  
Soft Rot ◽  
Pectobacterium Carotovorum ◽  
Bacterial Isolates ◽  
Biochemical Tests ◽  
Carotovorum Subsp ◽  
O 15 ◽  
O 69 ◽  
Physiological And Biochemical

A study was undertaken for characterization and identification of the soft rot causing bacterial pathogens of onion. Bacterial pathogens were isolated from soft rotted stored onions of different varieties and locations of Bangladesh. Altogether 73 bacterial isolates were isolated from soft rotted onions. Among them, twelve soft rot-positive isolates were selected for characterization and identification on the basis of more virulence. Physiological and biochemical tests were performed following standard methods for characterization and identification of selected soft rot bacterial isolates. Seven isolates namely O-03, O-18, O-69, O-72, O-130, O-156 and O-180 were identified as Pectobacterium carotovorum subsp. carotovorum (E. carotovora subsp. carotovora), two isolates O-101 and O-118 were identified as E. chrysanthemi and three isolates O-05, O-14 and O-15 were the members of Burkholderia cepacia.Bangladesh J. Sci. Ind. Res. 52(3), 209-220, 2017

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