Evaluation of quorum quenching Bacillus spp. for their biocontrol traits against Pectobacterium carotovorum subsp. carotovorum causing soft rot

2017 ◽  
Vol 9 ◽  
pp. 48-57 ◽  
Author(s):  
Sneha S. Garge ◽  
Anuradha S. Nerurkar
Keyword(s):  
Soft Rot ◽  
Quorum Quenching ◽  
Pectobacterium Carotovorum ◽  
Bacillus Spp ◽  
Carotovorum Subsp

scholarly journals First Report of Pectobacterium carotovorum subsp. brasiliense Causing Soft Rot on Squash and Watermelon in Serbia

Plant Disease ◽  
2019 ◽  
Vol 103 (10) ◽  
pp. 2667-2667 ◽  
Author(s):  
N. Zlatković ◽  
A. Prokić ◽  
K. Gašić ◽  
N. Kuzmanović ◽  
M. Ivanović ◽  
...  
Keyword(s):  
Soft Rot ◽  
Pectobacterium Carotovorum ◽  
First Report ◽  
Carotovorum Subsp

scholarly journals The Ribosomal Protein RplY Is Required for Pectobacterium carotovorum Virulence and Is Induced by Zantedeschia elliotiana Extract

2017 ◽  
Vol 107 (11) ◽  
pp. 1322-1330 ◽  
Author(s):  
Huan Jiang ◽  
Mengyi Jiang ◽  
Liuke Yang ◽  
Peiyan Yao ◽  
Lin Ma ◽  
...  
Keyword(s):  
Plant Extract ◽  
Plant Cell Wall ◽  
Insertion Site ◽  
Bacterial Pathogen ◽  
Soft Rot ◽  
Kanamycin Resistance ◽  
Pectobacterium Carotovorum ◽  
Promoter Trap ◽  
Carotovorum Subsp ◽  
Rot Disease

Pectobacterium carotovorum subsp. carotovorum strain PccS1, a bacterial pathogen causing soft rot disease of Zantedeschia elliotiana (colored calla), was investigated for virulence genes induced by the host plant. Using a promoter-trap transposon (mariner), we obtained 500 transposon mutants showing kanamycin resistance dependent on extract of Z. elliotiana. One of these mutants, PM86, exhibited attenuated virulence on both Z. elliotiana and Brassica rapa subsp. pekinensis. The growth of PM86 was also reduced in minimal medium (MM), and the reduction was restored by adding plant extract to the MM. The gene containing the insertion site was identified as rplY. The deletion mutant ΔrplY, exhibited reduced virulence, motility and plant cell wall-degrading enzyme production but not biofilm formation. Analysis of gene expression and reporter fusions revealed that the rplY gene in PccS1 is up-regulated at both the transcriptional and the translational levels in the presence of plant extract. Our results suggest that rplY is induced by Z. elliotiana extract and is crucial for virulence in P. carotovorum subsp. carotovorum.

scholarly journals First Report of Pectobacterium aroidearum and Pectobacterium carotovorum subsp. brasiliensis Causing Soft Rot of Cucurbita pepo in Brazil

Plant Disease ◽  
2017 ◽  
Vol 101 (2) ◽  
pp. 379 ◽  
Author(s):  
A. J. G. Moraes ◽  
E. B. Souza ◽  
R. L. R. Mariano ◽  
A. M. F. Silva ◽  
N. B. Lima ◽  
...  
Keyword(s):  
Cucurbita Pepo ◽  
Soft Rot ◽  
Pectobacterium Carotovorum ◽  
First Report ◽  
Carotovorum Subsp

Screening of quorum-quenching bacteria associated with rhizosphere as biocontrol agents of Pectobacterium carotovorum subsp. carotovorum

2020 ◽  
Vol 53 (11-12) ◽  
pp. 509-523 ◽  
Author(s):  
Fatemeh Alinejad ◽  
Fatemeh Shahryari ◽  
Omid Eini ◽  
Fatemeh Sarafraz-Niko ◽  
Ahmad Shekari ◽  
...  
Keyword(s):  
Quorum Quenching ◽  
Biocontrol Agents ◽  
Pectobacterium Carotovorum ◽  
Carotovorum Subsp

scholarly journals Emergence of Bacterial Soft Rot in Cucumber Caused by Pectobacterium carotovorum subsp. brasiliense in China

Plant Disease ◽  
2017 ◽  
Vol 101 (2) ◽  
pp. 279-287 ◽  
Author(s):  
Xianglong Meng ◽  
Ali Chai ◽  
Yanxia Shi ◽  
Xuewen Xie ◽  
Zhanhong Ma ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
Soft Rot ◽  
Economic Losses ◽  
Pectobacterium Carotovorum ◽  
Bacterial Soft Rot ◽  
Bacterial Strains ◽  
Phenotypic Properties ◽  
Gene Sequence Analysis ◽  
Wide Range ◽  
Carotovorum Subsp

During 2014 to 2015, a devastating bacterial soft rot on cucumber stems and leaves occurred in Shandong, Shanxi, Hebei, Henan, and Liaoning provinces of China, resulting in serious economic losses for cucumber production. The gummosis emerged on the surface of leaves, stems, petioles, and fruit of cucumber. The basal stem color was dark brown and the stem base turned to wet rot. Yellow spots and wet rot emerged at the edge of the infected cucumber leaves and gradually infected the leaf centers. In total, 45 bacterial strains were isolated from the infected tissues. On the basis of phenotypic properties of morphology, physiology, biochemistry, and 16S ribosomal RNA gene sequence analysis, the pathogen was identified as Pectobacterium carotovorum. Multilocus sequence analysis confirmed that the isolates were P. carotovorum subsp. brasiliense, and the pathogens fell in clade II. The pathogenicity of isolated bacteria strains was confirmed. The strains reisolated were the same as the original. The host range test confirmed that strains had a wide range of hosts. As far as we know, this is the first report of cucumber stem soft rot caused by P. carotovorum subsp. brasiliense in China as well as in the world, which has a significant economic impact on cucumber production.

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