scholarly journals Aeration and Stirring in Yarrowia lipolytica Lipase Biosynthesis during Batch Cultures with Waste Fish Oil as a Carbon Source

Fermentation ◽  
2021 ◽  
Vol 7 (2) ◽  
pp. 88
Author(s):  
Paulina Snopek ◽  
Dorota Nowak ◽  
Bartłomiej Zieniuk ◽  
Agata Fabiszewska
Keyword(s):  
Carbon Source ◽  
Fish Oil ◽  
Yarrowia Lipolytica ◽  
Lipolytic Activity ◽  
Culture Conditions ◽  
Agitation Speed ◽  
Oxygen Deficit ◽  
Shear Stresses ◽  
Process Selection ◽  
Batch Cultures

Yarrowia lipolytica is one of the most studied non-conventional forms of yeast, exhibiting a high secretory capacity and producing many industrially important and valuable metabolites. The yeast conceals a great biotechnological potential to synthesize organic acids, sweeteners, microbial oil, or fragrances. The vast majority of bioprocesses are carried out in bioreactors, where suitable culture conditions are provided. In the current study, the effect of agitation speed (200–600 rpm) and air flow rate (0.0375–2.0 dm3/(dm3 × min)) on the biomass yield and lipase activity of Y. lipolytica KKP 379 is analyzed in a growth medium containing waste fish oil. The increase of aeration intensity limited the period of oxygen deficit in the medium. Simultaneously, an increase in lipolytic activity was observed from 2.09 U/cm3 to 14.21 U/cm3; however, an excessive agitation speed likely caused oxidative or shear stresses, and a reduction in lipolytic activity was observed. Moreover, it is confirmed that the synthesis of lipases is related to oxygen consumption, pH, and the yeast growth phase, and appropriate process selection may provide two advantages, namely, the maximum use of the waste carbon source and the production of lipolytic enzymes that are valuable in many industries.

scholarly journals Studies on Upgradation of Waste Fish Oil to Lipid-Rich Yeast Biomass in Yarrowia lipolytica Batch Cultures

Foods ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 436
Author(s):  
Agata Urszula Fabiszewska ◽  
Bartłomiej Zieniuk ◽  
Mariola Kozłowska ◽  
Patrycja Maria Mazurczak-Zieniuk ◽  
Małgorzata Wołoszynowska ◽  
...  
Keyword(s):  
Fish Oil ◽  
Yarrowia Lipolytica ◽  
Ph Regulation ◽  
Culture Conditions ◽  
Waste Oil ◽  
Fish Waste ◽  
Batch Cultures ◽  
Yeast Biomass ◽  
Cellular Lipids ◽  
Positive Effect

The aim of the study was to evaluate the possibility to utilize a fish waste oil issued from the industrial smoking process in nitrogen-limited Yarrowia lipolytica yeast batch cultures. The waste carbon source was utilized by the yeast and stimulated the single cell oil production via an ex novo pathway. The yeast biomass contained lipids up to 0.227 g/g d.m.. Independently from culture conditions, high contents of very long chain fatty acids were quantified in yeast biomass including docosahexaenoic (DHA), eicosapentaenoic acid (EPA), eicosenic and erucic acids. The pH regulation did not influence the cellular lipids yield (0.234 g/g d.m.). Meanwhile, the intensification of the oxygenation of medium by changing the mixing speed (maximum concentration of lipids produced 4.64 g/dm3) and decreasing the amount of inoculum had a positive effect on the culture parameters in waste fish oil medium. Further work on upgradation of the original waste is advisable, especially because the oil indicated high content of polyphenols and lower susceptibility to oxidation than microbial oil derived from control olive oil medium.

scholarly journals The Overexpression of YALI0B07117g Results in EnhancedErythritol Synthesis from Glycerol by the Yeast Yarrowia lipolytica

Molecules ◽  
2021 ◽  
Vol 26 (24) ◽  
pp. 7549
Author(s):  
Mateusz Szczepańczyk ◽  
Dorota A. Rzechonek ◽  
Adam Dobrowolski ◽  
Aleksandra M. Mirończuk
Keyword(s):  
Carbon Source ◽  
Yarrowia Lipolytica ◽  
Sole Carbon Source ◽  
Consumption Rate ◽  
Batch Cultures ◽  
Cultivation Time ◽  
Erythrose Reductase ◽  
Yeast Yarrowia Lipolytica ◽  
Rate Of Consumption

The unconventional yeast Yarrowia lipolytica is used to produce erythritol from glycerol. In this study, the role of the erythrose reductase (ER) homolog YALI0B07117g in erythritol synthesis was analyzed. The deletion of the gene resulted in an increased production of mannitol (308%) and arabitol (204%) before the utilization of these polyols began. The strain overexpressing the YALI0B07117g gene was used to increase the erythritol yield from glycerol as a sole carbon source in batch cultures, resulting in a yield of 0.4 g/g. The specific consumption rate (qs) increased from 5.83 g/g/L for the WT strain to 8.49 g/g/L for the modified strain and the productivity of erythritol increased from 0.28 g/(L h) for the A101 strain to 0.41 g/(L h ) for the modified strain. The application of the research may prove positive for shortening the cultivation time due to the increased rate of consumption of the substrate combined with the increased parameters of erythritol synthesis.

scholarly journals Waste fish oil as an alternative carbon source in microbial oil production by Yarrowia lipolytica yeast

2019 ◽  
pp. 3-13
Author(s):  
Agata Fabiszewska ◽  
Patrycja Mazurczak-Zieniuk ◽  
Dorota Nowak ◽  
Małgorzata Wołoszynowska ◽  
Bartłomiej Zieniuk
Keyword(s):  
Carbon Source ◽  
Fish Oil ◽  
Yarrowia Lipolytica ◽  
Oil Production ◽  
Microbial Oil ◽  
Alternative Carbon Source

scholarly journals Synthesis of Industrially Useful Phenolic Compounds Esters by Means of Biocatalysts Obtained Along with Waste Fish Oil Utilization

2020 ◽  
Vol 12 (14) ◽  
pp. 5804 ◽  
Author(s):  
Bartłomiej Zieniuk ◽  
Małgorzata Wołoszynowska ◽  
Ewa Białecka-Florjańczyk ◽  
Agata Fabiszewska
Keyword(s):  
Phenolic Compounds ◽  
Fish Oil ◽  
Lipolytic Activity ◽  
Food Additives ◽  
Antioxidant Properties ◽  
Ethyl Esters ◽  
Whole Cell ◽  
Batch Cultures ◽  
Aromatic Alcohols ◽  
Oil Utilization

The purpose of the study was to utilize the waste fish oil after fish smoking process in Y. lipolytica yeast batch cultures in order to assess its valorization to whole-cell biocatalysts with lipolytic properties. Almost 90% of the waste carbon source in medium was used and batch cultures of Y. lipolytica W29 were characterized with biomass yield of 18.59 g/dm3 and lipolytic activity of 53.41 U/g. The yeast biomass was used as a whole-cell biocatalyst in the synthesis of esters of selected phenolic compounds (acetates of 2-phenylethanol, tyrosol (2-(4-hydroxyphenyl) ethanol) and 3-phenyl-1-propanol, and ethyl esters of phenylacetic, 4-hydroxyphenylacetic, 3-phenylpropanoic, and 3-(4-hydroxyphenyl) propanoic acids). Y. lipolytica biomass was able to catalyze esterification of 3-phenylpropanoic acid (conversion of 95% after 24 h), but conversion of other acids to their esters was very weak, and in the case of transesterification of aromatic alcohols with vinyl acetate, conversions were 27%–63% after 24 h. Synthesized esters were tested prior potential activities as food additives. Esters of hydroxylated phenolic compounds showed activity of 2.5 mg/mL against Klebsiella pneumoniae and Citrobacter freundii, exhibited similar antioxidant properties to their precursors, and were better soluble in lipids, which increases the possibility of their use in the food industry.

scholarly journals Lipids by Yarrowia lipolytica Strains Cultivated on Glucose in Batch Cultures

2020 ◽  
Vol 8 (7) ◽  
pp. 1054 ◽  
Author(s):  
Erdem Carsanba ◽  
Seraphim Papanikolaou ◽  
Patrick Fickers ◽  
Huseyin Erten
Keyword(s):  
Fatty Acids ◽  
Yarrowia Lipolytica ◽  
Lipid Production ◽  
Culture Conditions ◽  
Microbial Lipid ◽  
Culture Collections ◽  
Batch Cultures ◽  
Food Biotechnology ◽  
High Lipid ◽  
Dry Cell Weight

Oleaginous microorganisms, such as Yarrowia lipolytica, accumulate lipids that can have interesting applications in food biotechnology or the synthesis of biodiesel. Y. lipolytica yeast can have many advantages such as wide substrate range usage and robustness to extreme conditions, while under several culture conditions it can produce high lipid productivity. Based on this assumption, in this study, 12 different Yarrowia lipolytica strains were used to investigate microbial lipid production using a glucose-based medium under nitrogen-limited conditions in shake-flask cultivations. Twelve wild-type or mutant strains of Yarrowia lipolytica which were newly isolated or belonged to official culture collections were tested, and moderate lipid quantities (up to 1.30 g/L) were produced; in many instances, nitrogen limitation led to citric acid production in the medium. Lipids were mainly composed of C16 and C18 fatty acids. Most of the fatty acids of the microbial lipid were unsaturated and corresponded mainly to oleic, palmitic and linoleic acids. Linolenic acid (C18:3) was produced in significant quantities (between 10% and 20%, wt/wt of dry cell weight (DCW)) by strains H917 and Po1dL.

scholarly journals Transcriptome comparisons of in vitro intestinal epithelia grown under static and microfluidic gut-on-chip conditions with in vivo human epithelia

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kornphimol Kulthong ◽  
Guido J. E. J. Hooiveld ◽  
Loes Duivenvoorde ◽  
Ignacio Miro Estruch ◽  
Victor Marin ◽  
...  
Keyword(s):  
Gene Expression ◽  
Culture Conditions ◽  
Gene Set Enrichment Analysis ◽  
Shear Stresses ◽  
Static Culture ◽  
Dynamic Culture ◽  
Intestinal Segments ◽  
On Chip

AbstractGut-on-chip devices enable exposure of cells to a continuous flow of culture medium, inducing shear stresses and could thus better recapitulate the in vivo human intestinal environment in an in vitro epithelial model compared to static culture methods. We aimed to study if dynamic culture conditions affect the gene expression of Caco-2 cells cultured statically or dynamically in a gut-on-chip device and how these gene expression patterns compared to that of intestinal segments in vivo. For this we applied whole genome transcriptomics. Dynamic culture conditions led to a total of 5927 differentially expressed genes (3280 upregulated and 2647 downregulated genes) compared to static culture conditions. Gene set enrichment analysis revealed upregulated pathways associated with the immune system, signal transduction and cell growth and death, and downregulated pathways associated with drug metabolism, compound digestion and absorption under dynamic culture conditions. Comparison of the in vitro gene expression data with transcriptome profiles of human in vivo duodenum, jejunum, ileum and colon tissue samples showed similarities in gene expression profiles with intestinal segments. It is concluded that both the static and the dynamic gut-on-chip model are suitable to study human intestinal epithelial responses as an alternative for animal models.

scholarly journals Bioproduction of succinic acid from xylose by engineered Yarrowia lipolytica without pH control

2020 ◽  
Author(s):  
Ashish Prabhu ◽  
Rodrigo Ledesma- Amaro ◽  
Carol Sze Ki Lin ◽  
Frederic Coulon ◽  
Vijay kumar Thakur ◽  
...  
Keyword(s):  
Cell Growth ◽  
Carbon Source ◽  
Succinic Acid ◽  
Yarrowia Lipolytica ◽  
Recombinant Strain ◽  
Sole Carbon Source ◽  
Substrate Inhibition ◽  
Biomass Concentration ◽  
Value Added ◽  
Green Economy

Abstract Background Xylose is a most prevalent sugar available in hemicellulose fraction of lignocellulosic biomass (LCB) and of great interest for the green economy. Unfortunately, most of the cell factories cannot inherently metabolize xylose as sole carbon source. Yarrowia lipolytica is a non-conventional yeast to produce industrially important metabolites, and it is able to metabolize a large variety of substrates including both hydrophilic and hydrophobic carbon sources. However, Y. lipolytica lacks effective metabolic pathway for xylose uptake and only scarce information is available on utilization of xylose. For the economically feasible of LCB-based biorefineries, effective utilization of both pentose and hexose sugars is obligatory. Results In the present study, succinic acid (SA) production from xylose by Y. lipolytica was examined. To this end, Y. lipolytica PSA02004 strain was engineered by overexpressing pentose pathway cassette comprising of xylose reductase ( XR ), xylitol dehydrogenase ( XDH ) and xylulose kinase ( XK ) gene. The recombinant strain exhibited a robust growth on xylose as sole carbon source and accumulated SA (3.8 g/L) with a yield of 0.19 g/g in shake flask studies. Substrate inhibition studies revealed a marked negative impact on cell growth and product formation above 60 g/L xylose concentration. The modelling based on inhibition kinetics revealed that Aiba model showed better fit with experimental data, which resulted the correlation coefficient (R 2 ) of 0.82 and inhibition constant (K I ) 88.9 g/L. The batch cultivation of recombinant strain in bioreactor resulted in a maximum biomass concentration of 7.3 g/L and SA titer of 11.2 g/L with the yield of 0.18 g/g. Similar results in term of cell growth and SA production were obtained with xylose-rich hydrolysate derived from sugarcane bagasse. The fed-batch fermentation yielded biomass concentration of 11.8 g/L (OD 600 : 56.1) and SA titer of 22.3 g/L with a gradual decrease in pH below 4.0. Acetic acid was obtained as a main byproduct in all the fermentations. Conclusion The recombinant strain displayed potential bioconversion of xylose to succinic acid. Further this study provided a new insight on conversion of LCB into value-added products. To the best of our knowledge, this is the first study on SA production by Y. lipolytica using xylose as a sole carbon source.

scholarly journals Effect of culture conditions on nitrogen-fixing activity of bacteria isolated from cassava cultivated soils of Vietnam

2021 ◽  
Vol 43 (3) ◽  
pp. 27-35
Author(s):  
Pham Viet Cuong ◽  
Nguyen Phuong Hoa
Keyword(s):  
Bacillus Subtilis ◽  
Carbon Source ◽  
16S Rrna ◽  
Culture Conditions ◽  
Rrna Gene ◽  
Atmospheric Nitrogen ◽  
Bacillus Sp ◽  
Nitrogen Fixing ◽  
The 16S Rrna Gene ◽  
Cultivated Soils

The bacteria capable of fixing atmospheric nitrogen were isolated from cassava cultivated soils of Vietnam. The potential isolates were identified by analyzing the 16S rRNA gene and by morphological, biochemical, cultural characteristics. The selected isolates were assigned to the species Bacillus sp. DQT2 M17, Bacillus subtilis DTAN6 M17, and Bacillus megaterium DSHB I8. The effect of culture conditions on the nitrogen-fixing activity of three selected isolates were studied and the obtained results showed that the highest amount of accumulated ammonia was detected after 6 days of incubation at 35 oC, pH 7.0 with sucrose as a carbon source. The selected strains could be exploited as inoculants for microbial fertilizer production.

scholarly journals Screening of Amazonian bacillus strains for lipase production using glycerol as substrate

ScientiaTec ◽  
2020 ◽  
Vol 7 (03) ◽  
Author(s):  
Giandra Volpato ◽  
Victória Furtado Migliavacca ◽  
Bruna Coelho de Andrade ◽  
Júlio Xandro Heck ◽  
Marco Antônio Záchia Ayub
Keyword(s):  
Organic Synthesis ◽  
Culture Medium ◽  
Lipolytic Activity ◽  
Gum Arabic ◽  
Lipase Production ◽  
Culture Conditions ◽  
Search And Selection ◽  
Triton X ◽  
Potential Use ◽  
Selection Of

The industrial application of lipolytic enzymes has been studied mainly due to the ability of these enzymes in catalyze reactions of synthesis and their stability in various organic solvents. One possibility is the use of lipase the organic synthesis, taking advantage as the generation of waste and difficult recovery of sub bioproducts. In this work, we carried out a selection of eighty-four isolates of Bacillus amazonian for lipase production, of which 30 strains showed lipolytic activity. The study of the culture conditions was performed through a Plackett-Burman experimental design using the strain that presented the highest lipolytic activity in a culture medium using glycerol as substrate.  The studied conditions were: concentration of soybean oil, olive oil, triton X-100, gum arabic, glycerol, and (NH4)2SO4, pH, temperature and concentration of inoculums. The best result obtained were 27 U/L in 48 h of cultivation by Bacillus circulans BL53. This work shows that the search and selection of microorganism with lipolytic activities can facilitate the discovery of new lipases, with potential use as by-product surplus.

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