scholarly journals Co-Inoculation of Bacillus velezensis Strain S141 and Bradyrhizobium Strains Promotes Nodule Growth and Nitrogen Fixation

2020 ◽  
Vol 8 (5) ◽  
pp. 678
Author(s):  
Surachat Sibponkrung ◽  
Takahiko Kondo ◽  
Kosei Tanaka ◽  
Panlada Tittabutr ◽  
Nantakorn Boonkerd ◽  
...  
Keyword(s):  
N2 Fixation ◽  
Growth Promotion ◽  
Nodule Formation ◽  
Bacillus Velezensis ◽  
Cytokinin Biosynthesis ◽  
Large Size ◽  
Other Substances ◽  
Glycine Max L ◽  
Iaa Biosynthesis

The objective of this research was to evaluate the PGPR effect on nodulation and nitrogen-fixing efficiency of soybean (Glycine max (L.) Merr.) by co-inoculation with Bradyrhizobium diazoefficiens USDA110. Co-inoculation of Bacillus velezensis S141 with USDA110 into soybean resulted in enhanced nodulation and N2-fixing efficiency by producing larger nodules. To understand the role of S141 on soybean and USDA110 symbiosis, putative genes related to IAA biosynthesis were disrupted, suggesting that co-inoculation of USDA110 with S141ΔyhcX reduces the number of large size nodules. It was revealed that yhcX may play a major role in IAA biosynthesis in S141 as well as provide a major impact on soybean growth promotion. The disruption of genes related to cytokinin biosynthesis and co-inoculation of USDA110 with S141ΔIPI reduced the number of very large size nodules, and it appears that IPI might play an important role in nodule size of soybean–Bradyrhizobium symbiosis. However, it was possible that not only IAA and cytokinin but also some other substances secreted from S141 facilitate Bradyrhizobium to trigger bigger nodule formation, resulting in enhanced N2-fixation. Therefore, the ability of S141 with Bradyrhizobium co-inoculation to enhance soybean N2-fixation strategy could be further developed for supreme soybean inoculants.

scholarly journals The Role of Rhizobial ACC Deaminase in the Nodulation Process of Leguminous Plants

2016 ◽  
Vol 2016 ◽  
pp. 1-9 ◽  
Author(s):  
Francisco X. Nascimento ◽  
Clarisse Brígido ◽  
Bernard R. Glick ◽  
Márcio J. Rossi
Keyword(s):  
Stress Responses ◽  
Higher Plants ◽  
Growth Promotion ◽  
Agricultural Practices ◽  
Acc Deaminase ◽  
Nodule Development ◽  
Nodule Formation ◽  
Direct Role ◽  
Sustainable Agricultural Practices

Symbiotic rhizobia-legumes associations are extremely important in terms of sustainable agricultural practices. This symbiosis involves a complex interaction between both partners, plant and bacterium, for bacterial infection and the formation of symbiotic N-fixing nodules. In this regard, the phytohormone ethylene plays a significant role in nodule formation, acting as an inhibitor of the nodulation process. Ethylene not only regulates nodule development but also regulates many other plant developmental cues, including various stress responses that inhibit overall plant growth. Some rhizobia produce the enzyme 1-aminocyclopropane-1-carboxylate (ACC) deaminase, thus, being able to decrease ACC and, consequently, decrease deleterious ethylene levels that affect the nodulation process. This occurs because ACC is the immediate precursor of ethylene in all higher plants. Hence, rhizobia that express this enzyme have an increased symbiotic potential. In addition to the direct role that ACC deaminase plays in the nodulation processper se, in a limited number of instances, ACC deaminase can also modulate nodule persistence. This review focuses on the important role of rhizobial ACC deaminase during the nodulation process, emphasizing its significance to legume growth promotion.

scholarly journals The Role of Auxins and Cytokinins in the Mutualistic Interaction Between Arabidopsis and Piriformospora indica

2008 ◽  
Vol 21 (10) ◽  
pp. 1371-1383 ◽  
Author(s):  
Jyothilakshmi Vadassery ◽  
Claudia Ritter ◽  
Yvonne Venus ◽  
Iris Camehl ◽  
Ajit Varma ◽  
...  
Keyword(s):  
Root Colonization ◽  
Growth Promotion ◽  
Growth Stimulation ◽  
Piriformospora Indica ◽  
Dwarf Phenotype ◽  
Cytokinin Biosynthesis ◽  
Cytokinin Receptor ◽  
Mutualistic Interaction ◽  
Growth And Reproduction

Arabidopsis growth and reproduction are stimulated by the endophytic fungus Piriformospora indica. The fungus produces low amounts of auxins, but the auxin levels and the expression of auxin-regulated genes are not altered in colonized roots. Also, mutants with reduced auxin levels (ilr1-1, nit1-3, tfl2, cyp79 b2b3) respond to P. indica. However, the fungus rescues the dwarf phenotype of the auxin overproducer sur1-1 by converting free auxin into conjugates, which also results in the downregulation of the auxin-induced IAA6 and the upregulation of the P. indica–induced LRR1 gene. The fungus produces relatively high levels of cytokinins, and the cytokinin levels are higher in colonized roots compared with the uncolonized controls. trans-Zeatin cytokinin biosynthesis and the CRE1/AHK2 receptor combination are crucial for P. indica–mediated growth stimulation, while mutants lacking cis-zeatin, impaired in other cytokinin receptor combinations, or containing reduced cytokinin levels respond to the fungus. Since root colonization is not affected in the cytokinin mutants, we propose that cytokinins are required for P. indica–induced growth promotion. Finally, a comparative analysis of the phytohormone mutants allows the conclusion that the response to P. indica is independent of the architecture and size of the roots.

Central ossifying fibroma of mandible

2020 ◽  
Vol 13 (12) ◽  
pp. e239286
Author(s):  
Kumar Nilesh ◽  
Prashant Punde ◽  
Nitin Shivajirao Patil ◽  
Amol Gautam
Keyword(s):  
Fibrous Tissue ◽  
Three Dimensional ◽  
Facial Asymmetry ◽  
Ossifying Fibroma ◽  
Mandible Reconstruction ◽  
Three Dimensional Printing ◽  
Osseous Lesion ◽  
Large Size ◽  
Dimensional Printing

Ossifying fibroma (OF) is a rare, benign, fibro-osseous lesion of the jawbone characterised by replacement of the normal bone with fibrous tissue. The fibrous tissue shows varying amount of calcified structures resembling bone and/or cementum. The central variant of OF is rare, and shows predilection for mandible among the jawbone. Although it is classified as fibro-osseous lesion, it clinically behaves as a benign tumour and can grow to large size, causing bony swelling and facial asymmetry. This paper reports a case of large central OF of mandible in a 40-year-old male patient. The lesion was treated by segmental resection of mandible. Reconstruction of the surgical defect was done using avascular fibula bone graft. Role of three-dimensional printing of jaw and its benefits in surgical planning and reconstruction are also highlighted.

Promotion of stipe elongation in isolated Flammulina velutipes fruit bodies by carbohydrates, natural extracts, and amino acids

1972 ◽  
Vol 50 (4) ◽  
pp. 803-818 ◽  
Author(s):  
Hans E. Gruen ◽  
Sheue-heng Wu
Keyword(s):  
Amino Acids ◽  
Growth Promotion ◽  
Casein Hydrolysate ◽  
Growth Period ◽  
Flammulina Velutipes ◽  
Ammonium Tartrate ◽  
Acid Growth ◽  
Natural Extracts ◽  
Stipe Elongation ◽  
Other Substances

Isolated Flammulina velutipes fruit bodies were cultured under sterile conditions with the cut base immersed in water or solutions. Stipe elongation on water was only 6% of normal for fruit bodies isolated at 1.1–2.0 cm length, 19% at 5.1–6.0 cm, and the same as for fruit bodies attached to mycelium at 9.1–10.0 cm. Fruit bodies not immersed in water grew less in a saturated atmosphere than those in water. The mycelium must supply other substances than water for normal elongation during about two-thirds of the growth period, and only water thereafter. Isolated fruit bodies fed with filtered glucose, trehalose, sucrose, or mannitol grew better than on water. Maltose and fructose increased elongation only slightly, and sorbose had no effect. Potato extract, yeast extract, and casein hydrolysate gave no or very little growth promotion, but addition of glucose strongly increased growth on the natural extracts compared to glucose alone. Of 21 amino acids added separately to glucose, only asparagine, hydroxyproline, arginine, and to a lesser extent glutamine, stimulated growth of isolated fruit bodies. Growth was not promoted by pure asparagine, glutamine, and serine, or by thiamin or indoleacetic acid. Growth was inhibited by urea, ammonium nitrate, and ammonium tartrate with or without glucose.Growth promoting substances were most effective in young fruit bodies and except for glucose the promotion disappeared in fruit bodies isolated at 6.1–7.0 cm length, which corresponds to the end of the period of rapid elongation. Apical portions of fruit bodies with caps grew better on glucose than whole fruit bodies. Growth of decapitated isolated stipes was not promoted by nutrients.

Pearls From Bones: Relics, Chortens, Tertons and the Signs of Saintly Death in Tibet

Numen ◽  
1994 ◽  
Vol 41 (3) ◽  
pp. 273-324 ◽  
Author(s):  
Dan Martin
Keyword(s):  
Historical Perspective ◽  
Religious Studies ◽  
Religious Culture ◽  
Tibetan Buddhist ◽  
Devotional Practice ◽  
Cultural Pattern ◽  
Other Substances ◽  
Critical Links ◽  
Point Of Departure

AbstractAlthough there has been much work, in recent years, on the sacrum of Christianity, and some important studies have appeared on Buddhist relic cults and related facets of Buddhism, so far very little has been written on Tibetan Buddhist relics. This paper, while offering some material for a historical perspective, mainly seeks to find a larger cultural pattern for understanding the interrelationships of a complex of factors active in Tibetan religious culture. Beginning with problems of relic-related terms and classifications, we then suggest a new assessment of the role of the Terton ('treasure revealer'). Then we discuss 'miracles' in Tibet, and the intersection of categories of 'signs of saintly death' and relics. Much of the remaining pages are devoted to those items that fall within both categories, specifically the 'pearls' that emerge miraculously from saintly remains and images that appear in bodily or other substances connected with cremations. After looking at a number of testimonials on these miraculous relics, we examine the possibility that these items might be 'deceitfully manufactured', looking at a few Tibetan polemical writings which raise this possibility. In the conclusion, we suggest that there are some critical links between three spheres of Tibetan religiosity: 1. sacrum which are not relics, 2. relics, and 3. signs of sainthood. Finally, we recommend an approach to religious studies that takes its point of departure in actual practices, and particularly the objects associated with popular devotional practice.

THE ROLE OF THE MOTHER IN 131I METABOLISM OF SUCKING AND WEANLING RATS

1965 ◽  
Vol 43 (3) ◽  
pp. 431-436 ◽  
Author(s):  
M. Samel ◽  
A. Caputa
Keyword(s):  
Urinary Bladder ◽  
In Utero ◽  
The State ◽  
Newborn Rats ◽  
Weanling Rats ◽  
Immature Rats ◽  
Other Substances ◽  
Old Rats

In newborn rats the mother provokes the emptying of the urinary bladder by stimulating the perineum with her tongue. The possibility that mothers may thereby ingest the urine of their young has been studied by means of 131I on nine litters of rats aged 10 to 29 days. The results indicate that a considerable quantity of 131I administered intraperitoneally to 10- and 18-day-old rats, which were then reunited with their mothers for 4 hours, reappears in the organism of uninjected nurslings after passing through the organism of the mother. The amount of 131I transferred from injected rats into the bodies of isolated uninjected rats of the same litter decreased during the period of weaning. The observed recirculation of 131I between immature rats and their mothers in both directions may represent a saving mechanism which might include several other substances and would compensate for their loss via the milk, and suggests a new aspect of maternal–neonatal interrelationship which appears as a continuation of the state existing in utero.

N2-Fixation and Seedling Growth Promotion of Lodgepole Pine by Endophytic Paenibacillus polymyxa

2013 ◽  
Vol 66 (2) ◽  
pp. 369-374 ◽  
Author(s):  
Richa Anand ◽  
Susan Grayston ◽  
Christopher Chanway
Keyword(s):  
Seedling Growth ◽  
N2 Fixation ◽  
Growth Promotion ◽  
Lodgepole Pine ◽  
Paenibacillus Polymyxa

scholarly journals Combined Application of Rhizosphere Bacteria with Endophytic Bacteria Suppresses Root Diseases and Increases Productivity of Black Pepper (Piper nigrum L.)

Agriculture ◽  
2020 ◽  
Vol 11 (1) ◽  
pp. 15
Author(s):  
Sy Dinh Nguyen ◽  
Thi Huyen Trang Trinh ◽  
Trung Dzung Tran ◽  
Tinh Van Nguyen ◽  
Hoang Van Chuyen ◽  
...  
Keyword(s):  
Bacillus Subtilis ◽  
Endophytic Bacteria ◽  
Growth Promotion ◽  
Black Pepper ◽  
Rhizosphere Bacteria ◽  
Growth And Yield ◽  
Piper Nigrum ◽  
Bacillus Velezensis ◽  
Root Knot Nematode ◽  
Central Highland

Black pepper (Piper nigrum L.) is one of the most important crops and global demand continues to increase, giving it a high export value. However, black pepper cultivation has been seriously affected by a number of pathogenic diseases. Among them, “quick wilt” caused by Phytophthora sp., “slow decline” caused by Fusarium sp., and root-knot nematode Meloidogyne sp. have a serious negative effect on black pepper growth and productivity. There have been different chemical and biological methods applied to control these diseases, but their effectiveness has been limited. The aim of this research was to evaluate different combinations of rhizosphere bacteria and endophytic bacteria isolated from black pepper farms in the Central Highland of Vietnam for their ability to suppress pathogens and promote black pepper growth and yield. Formula 6, containing the strains Bacillus velezensis KN12, Bacillus amyloliquefaciens DL1, Bacillus velezensis DS29, Bacillus subtilis BH15, Bacillus subtilis V1.21 and Bacillus cereus CS30 exhibited the largest effect against Phytophthora and Fusarium in the soil and in the roots of black pepper. These bio-products also increased chlorophyll a and b contents, which led to a 1.5-fold increase of the photosynthetic intensity than the control formula and a 4.5% increase in the peppercorn yield (3.45 vs. 3.30 tons per hectare for the control). Our results suggest that the application of rhizosphere and endophytic bacteria is a promising method for disease control and growth-promotion of black pepper.

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