scholarly journals Listeria monocytogenes in Export-approved Beef from Mato Grosso, Brazil: Prevalence, Molecular Characterization and Resistance to Antibiotics and Disinfectants

2019 ◽  
Vol 8 (1) ◽  
pp. 18 ◽  
Author(s):  
Larrayane A.C. Teixeira ◽  
Fernanda T. Carvalho ◽  
Deyse C. Vallim ◽  
Rodrigo C.L. Pereira ◽  
Adelino Cunha Neto ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Listeria Monocytogenes ◽  
Antimicrobial Resistance ◽  
Multiplex Pcr ◽  
Molecular Characterization ◽  
Sodium Hypochlorite ◽  
High Resistance ◽  
Molecular Properties ◽  
Mato Grosso ◽  
Microbiological Safety

The Brazilian state of Mato Grosso is the largest producer and exporter of beef in the country, but few studies of relevance have been conducted to evaluate the microbiological safety of its products. This study aimed to estimate the prevalence of Listeria monocytogenes (LM) in export-approved beef from Mato Grosso and to characterize the isolates in terms of molecular properties and antimicrobial resistance. From a total of 50 samples analyzed, Listeria sp. was isolated in 18 (36% prevalence). Listeria monocytogenes was confirmed in 6 (12% prevalence). Among the serotype groups assessed by multiplex PCR, serotype 4 (4b, 4d or 4e) was the most prevalent. Although antibiotic resistance was not an issue, two strains isolated from different plants showed high resistance to sodium hypochlorite. Overall, this scenario causes concern because it puts at risk not only the Brazilian customer, but also the population of countries that import beef from Mato Grosso.

scholarly journals Clostridium perfringens spores in Polish honey samples

2018 ◽  
Vol 62 (3) ◽  
pp. 281-284 ◽  
Author(s):  
Tomasz Grenda ◽  
Magdalena Grabczak ◽  
Magdalena Goldsztejn ◽  
Nina Kozieł ◽  
Krzysztof Kwiatek ◽  
...  
Keyword(s):  
Risk Assessment ◽  
Antibiotic Resistance ◽  
Multiplex Pcr ◽  
Clostridium Perfringens ◽  
Potential Risk ◽  
Microbiological Safety ◽  
Pcr Method ◽  
Harvesting Process ◽  
Made In

AbstractIntroductionThe aim of this study was examination of honey samples collected from apiaries situated in all Polish provinces for occurrence ofClostridiumspp., especiallyC. perfringens.Material and MethodsThe study was carried out on 240 honey samples (15 samples/province). Estimation ofClostridiumtitre, its cultures andC. perfringensisolate characterisation were performed according to the standard PN-R-64791:1994. A multiplex PCR method for detection of genes codingcpa(α toxin),cpb(β),cpb2(β2),etx(ε),iap(ι), andcpe(enterotoxin) toxins was used.ResultsClostridiumspp. was noticed in 56% (136/240) of samples, and its titres ranged between 0.1 g and 0.001 g.Clostridium perfringensoccurrence was evidenced in 27.5% (66/240) of samples. All isolates were classified to toxinotype A.ConclusionsEvidence of a high number of positive samples with occurrence ofClostridiumspp. indicates a potential risk to consumers’ health. The infective number ofClostridiumspp. is unknown; however, the obtained results have shown that a risk assessment on the entire honey harvesting process should be made in order to ensure microbiological safety. Moreover, a detailed study should be undertaken on the antibiotic resistance ofC. perfringensisolates from honey samples.

scholarly journals Antimicrobial and disinfectant resistance of Salmonella Heidelberg from Brazilian flocks did not increase for ten years (2006-2016)

2021 ◽  
Vol 41 ◽  
Author(s):  
Juliana Bassani ◽  
Mariana Paravisi ◽  
Daiane E. Wilsmann ◽  
Karen A. Borges ◽  
Thales Q. Furian ◽  
...  
Keyword(s):  
Antimicrobial Resistance ◽  
Sodium Hypochlorite ◽  
High Resistance ◽  
Benzalkonium Chloride ◽  
Antimicrobial Agents ◽  
Food Products ◽  
Foodborne Illness ◽  
Poultry Farm ◽  
Disinfectant Resistance ◽  
Salmonella Heidelberg

ABSTRACT: Salmonella is a major cause of foodborne illness worldwide, and poultry and its derived products are the most common food products associated with salmonellosis outbreaks. Some countries, including Brazil, have experienced an increased prevalence of Salmonella Heidelberg among their poultry flocks. Some isolates have also presented high resistance to antimicrobial agents and persist in the poultry farm environment. This study aimed to compare the susceptibility of S. Heidelberg strains isolated in 2006 with those isolated in 2016 against disinfectants and antimicrobial agents. The results showed that all the strains were highly susceptible to sodium hypochlorite, regardless of the conditions and year of isolation. Resistance to benzalkonium chloride varied according to the conditions applied, but not to the year of isolation. Increased antimicrobial resistance from 2006-2016 was observed only for tetracycline. The results suggest that the antimicrobial and disinfectant resistance of S. Heidelberg did not increase for ten years (2006-2016). However, further analysis should include a larger number of S. Heidelberg isolates from poultry origin and additional antimicrobial agents for more precise conclusions about the increasing in antimicrobial resistance in the last years.

Prevalence and Antimicrobial Resistance of Listeria monocytogenes Isolated in Chicken Slaughterhouses in Northern Greece

2011 ◽  
Vol 74 (6) ◽  
pp. 1017-1021 ◽  
Author(s):  
I. SAKARIDIS ◽  
N. SOULTOS ◽  
E. IOSSIFIDOU ◽  
A. PAPA ◽  
I. AMBROSIADIS ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Listeria Monocytogenes ◽  
Antimicrobial Resistance ◽  
Nalidixic Acid ◽  
Random Amplified Polymorphic Dna ◽  
The Other ◽  
Diffusion Method ◽  
Northern Greece ◽  
Disk Diffusion Method ◽  
High Prevalence

This study was conducted to determine the prevalence and antimicrobial resistance of Listeria monocytogenes recovered from chicken carcasses in slaughterhouses in Northern Greece. A total of 100 poultry samples (300 carcasses) were examined for Listeria spp. The samples were neck skin taken from four different slaughterhouses in Northern Greece. Forty samples were also taken from the environment of the slaughterhouses. Identification of L. monocytogenes was carried out by PCR and fingerprinting of the isolates by random amplified polymorphic DNA. L. monocytogenes strains isolated from chicken carcasses and from the environment of the slaughterhouses were also examined for antibiotic resistance. Fifty-five isolates of L. monocytogenes were tested for susceptibility to 20 antibiotics using the disk diffusion method. Listeria spp. were present in 99 of the poultry samples tested (99%), and 38 yielded L monocytogenes (38%). L. monocytogenes was also isolated in 80% of samples from the environment of a certain slaughterhouse, while the other slaughterhouses were found to be contaminated only with Listeria spp. All isolates were resistant to nalidixic acid and oxolinic acid, the majority of them to clindamycin, and only a few to tetracycline and oxytetracycline, whereas they were found to be susceptible to all other antimicrobials. The results of this study demonstrate a high prevalence of L. monocytogenes contamination in chicken carcasses, and all isolates were found to be sensitive to the antimicrobials most commonly used to treat human listeriosis.

scholarly journals Prevalencia de especies zoonóticas de Arcobacter en cerdos a nivel de matadero en Ecuador

2020 ◽  
pp. e1798
Author(s):  
Rosa Janneth Simaluiza-Masabanda ◽  
Jimmy Fernández-Cajamarca ◽  
Heriberto Fernandez
Keyword(s):  
Antimicrobial Resistance ◽  
Multiplex Pcr ◽  
High Resistance ◽  
Probabilistic Method ◽  
Diffusion Method ◽  
Disk Diffusion Method ◽  
Fecal Carriage ◽  
Microbiological Methods ◽  
Southern Ecuador ◽  
Resistant Strains

Objective. To establish the prevalence, diversity and antimicrobial resistance of the zoonotic species of Arcobacter in healthy pigs at slaughterhouse level. Material and Methods. Fifty fecal samples were taken by rectal swabs from healthy pigs, before the beginning of the slaughter at the slaughterhouse of Loja city, Southern Ecuador. Sampling was performed using a non-probabilistic method for convenience. Isolation of Arcobacter strains was done by microbiological methods and species identification using biochemical and molecular (multiplex PCR) tests. Antimicrobial behavior was performed using the disk diffusion method. Results. The four zoonotic species of Arcobacter were found. The isolation rates were A. thereius (18.0%), A. skirrowii (18.0%), A. cryaerophilus (6.0%) and A. butzleri (2.0%). High resistance to ciprofloxacin was found and multi-resistant strains were isolated from these four species. Conclusions. The fecal carriage of the zoonotic species of Arcobacter was demonstrated in pigs at slaughterhouse level. These species showed high resistance to ciprofloxacin being isolated muti-resistant strains among these four species.

Molecular Characterization of Antibiotic Resistance Gene Pattern of Staphylococcus aureus and Escherichia coli in Mastitis Affected Dairy Cows

2020 ◽  
Author(s):  
T. Ramasamy ◽  
S. Keerthana ◽  
M.R. Srinivasan ◽  
D. Chandrasekar ◽  
K. Porteen ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Molecular Characterization ◽  
Antibiotic Sensitivity ◽  
Growth Media ◽  
E Coli ◽  
Sensitivity Pattern

Background: Antimicrobial resistance is one of the latest challenges facing the scientific community. Raising the drug resistance is caused mainly by indiscriminate usage of antibiotics in human and animal subjects and the spread of antibiotic resistance between the two has an emerging global threat. Hence, current study aimed to study the antimicrobial resistance pattern and molecular detection of antibiotic resistance genes in Staphylococcus aureus and Escherichia coli isolated from mastitis affected cows. Methods: Milk samples from mastitis affected cows were subjected to antibiotic sensitivity test and screened for presence of Staphylococcus aureus and Escherichia coli using differential growth media. Molecular characterization of Staphylococcus aureus and Escherichia coli was done with the help of PCR by amplification of ‘nuc’ and ‘uspA’ gene respectively. MICs of Penicillin and Tetracycline were determined using microdilution method. Result: Antibiotic sensitivity pattern for Penicillin G, Ampicillin, Amoxycillin, Cefotaxime, Ceftriaxone, Azithromycin, Ciprofloxacin, Gentamicin, Oxytetracycline, Tetracycline and Vancomycin were 74.19%, 100%, 93.50%, 61.29%, 29%, 35.48%, 9.70%, 9.70%, 70.96% and 70.96% respectively. More than 87.90% of the S. aureus and 50% of the E. coli isolated were resistant to â-lactam antibiotics while 75% of the E.coli and 65.70% of the S. aureus isolated were resistant to Tetracycline antibiotics. The MICs of Penicillin for S.aureus and E.coli are 26.88 µg/ml and 13.54 µg/ml respectively and the MICs of Tetracycline for S. aureus and E. coli are 243.75 µg/ml and 960.93 µg/ml respectively which is 8-9 folds higher than the standard MICs. From the present study, it can be inferred that bovine mastitis cases are highly resistant to antimicrobial drugs. Results further indicate that Staphylococcus aureus and Escherichia coli are both resistant to Penicillin and Tetracycline with very high MIC.

Genetic similarity, antibiotic resistance and disinfectant susceptibility of Listeria monocytogenes isolated from chicken meat and chicken-meat processing environment in Mato Grosso, Brazil

LWT ◽  
2019 ◽  
Vol 109 ◽  
pp. 77-82 ◽  
Author(s):  
Fernanda T. Carvalho ◽  
Bruno S. Vieira ◽  
Deyse C. Vallim ◽  
Larrayane A. Carvalho ◽  
Ricardo C.T. Carvalho ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Listeria Monocytogenes ◽  
Genetic Similarity ◽  
Chicken Meat ◽  
Meat Processing ◽  
Mato Grosso

scholarly journals High Prevalence of Antibiotic Resistance and Molecular Characterization of Integrons among Shigella Isolates in Eastern China

2013 ◽  
Vol 57 (3) ◽  
pp. 1549-1551 ◽  
Author(s):  
Yun Shen ◽  
Huimin Qian ◽  
Junjun Gong ◽  
Feifei Deng ◽  
Chen Dong ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Molecular Characterization ◽  
High Resistance ◽  
Eastern China ◽  
Jiangsu Province ◽  
Content Type ◽  
Resistant Genes ◽  
High Prevalence ◽  
Rapid Dissemination

ABSTRACTA total of 747Shigellaisolates were collected from hospitals in Jiangsu Province of China. Susceptibilities to antimicrobials and integrons were tested. A total of 78.3% ofS. flexneriisolates and a total of 74.3%S. soneiisolates were resistant to at least three antibiotics. Of theShigellaisolates, 74.7% had integron I and 82.6% had integron II. The conjunction of the high prevalence of integrons inShigellaand high resistance to antimicrobials will lead to rapid dissemination of resistant genes in this region.

scholarly journals Determination of Antibiotic Resistance Profile and Virulence Genes in Escherichia coli Isolates from Palestinian Patients

2019 ◽  
pp. 1-9
Author(s):  
Ghaleb Adwan
Keyword(s):  
Escherichia Coli ◽  
Antibiotic Resistance ◽  
Antimicrobial Resistance ◽  
Virulence Factors ◽  
High Resistance ◽  
Virulence Genes ◽  
Resistance Rate ◽  
E Coli ◽  
Different Types ◽  
Class 1

Introduction: Escherichia coli (E. coli) is considered one of the most frequent intestinal and extraintestinal pathogen. Methods:  A total of 49 isolates of E. coli were collected from different clinical samples, from different hospitals in Northern West Bank-Palestine, during January-March 2019. Aims: To detect the distribution of Type III secretion system (T3SS) genes, class 1, 2 and 3 integrons, virulence factors (fyuA, papGIII, iutA and sfa⁄foc) using multiplex PCR and antibiotic resistance using disc diffusion method. Results: In this study, E. coli isolates showed high resistance rate against different types of antibiotics and 71.4% of the isolates were multidrug resistant (MDR). Only class 1 integron was detected in these isolates with prevalence 57%, and 65.7% of MDR isolates carried integron genes. The prevalence of T3SS genes was 0.0%. In addition, results of this study showed that the prevalence of virulence genes papGIII, sfa⁄foc, fyuA and iutA was 4.1%, 40%, 64%, and 79.6%, respectively. Conclusions: The isolates of E. coli showed high resistance rate against different types of antibiotics. The co-occurrence of class 1 integrons and antimicrobial resistance genes in these isolates is an additional threat for spread of the antimicrobial resistance traits which may further complicate future strategies for treatment the infections caused by this pathogen. In addition, E. coli isolated from Palestinian patients showed one or more virulence factors that could increase their pathogenic potential.

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