scholarly journals Erratum: Farber, R.; Rosenberg, A.; Rozenfeld, S.; Banet, G.; Cahan, R. Bioremediation of Artificial Diesel-Contaminated Soil Using Bacterial Consortium Immobilized to Plasma-Pretreated Wood Waste. Microorganisms 2019, 7, 497

2019 ◽  
Vol 7 (12) ◽  
pp. 677 ◽  
Author(s):  
Ravit Farber ◽  
Alona Rosenberg ◽  
Shmuel Rozenfeld ◽  
Gabi Banet ◽  
Rivka Cahan

The authors wish to make the following erratum in this paper [...]

2019 ◽  
Vol 7 (11) ◽  
pp. 497 ◽  
Author(s):  
Ravit Farber ◽  
Alona Rosenberg ◽  
Shmuel Rozenfeld ◽  
Gabi Banet ◽  
Rivka Cahan

Bioaugmentation is a bioremediation option based on increasing the natural in-situ microbial population that possesses the ability to degrade the contaminating pollutant. In this study, a diesel-degrading consortium was obtained from an oil-contaminated soil. The diesel-degrading consortium was grown on wood waste that was plasma-pretreated. This plasma treatment led to an increase of bacterial attachment and diesel degradation rates. On the 7th day the biofilm viability on the plasma-treated wood waste reached 0.53 ± 0.02 OD 540 nm, compared to the non-treated wood waste which was only 0.34 ± 0.02. Biofilm attached to plasma-treated and untreated wood waste which was inoculated into artificially diesel-contaminated soil (0.15% g/g) achieved a degradation rate of 9.3 mg day−1 and 7.8 mg day−1, respectively. While, in the soil that was inoculated with planktonic bacteria, degradation was only 5.7 mg day−1. Exposing the soil sample to high temperature (50 °C) or to different soil acidity did not influence the degradation rate of the biofilm attached to the plasma-treated wood waste. The two most abundant bacterial distributions at the family level were Xanthomonadaceae and Sphingomonadaceae. To our knowledge, this is the first study that showed the advantages of biofilm attached to plasma-pretreated wood waste for diesel biodegradation in soil.


2019 ◽  
Vol 9 (22) ◽  
pp. 4757 ◽  
Author(s):  
Mikel Anza ◽  
Oihane Salazar ◽  
Lur Epelde ◽  
José María Becerril ◽  
Itziar Alkorta ◽  
...  

Here, we aimed to bioremediate organically contaminated soil with Brassica napus and a bacterial consortium. The bioaugmentation consortium consisted of four endophyte strains that showed plant growth-promoting traits (three Pseudomonas and one Microbacterium) plus three strains with the capacity to degrade organic compounds (Burkholderia xenovorans LB400, Paenibacillus sp. and Lysinibacillus sp.). The organically contaminated soil was supplemented with rhamnolipid biosurfactant and sodium dodecyl benzenesulfonate to increase the degradability of the sorbed contaminants. Soils were treated with organic amendments (composted horse manure vs. dried cow slurry) to promote plant growth and stimulate soil microbial activity. Apart from quantification of the expected decrease in contaminant concentrations (total petroleum hydrocarbons, polycyclic aromatic hydrocarbons), the effectiveness of our approach was assessed in terms of the recovery of soil health, as reflected by the values of different microbial indicators of soil health. Although the applied treatments did not achieve a significant decrease in contaminant concentrations, a significant improvement of soil health was observed in our amended soils (especially in soils amended with dried cow slurry), pointing out a not-so-uncommon situation in which remediation efforts fail from the point of view of the reduction in contaminant concentrations while succeeding to recover soil health.


2019 ◽  
Vol 19 (2) ◽  
pp. 347 ◽  
Author(s):  
Abubakar Tuhuloula ◽  
Suprapto Suprapto ◽  
Ali Altway ◽  
Sri Rachmania Juliastuti

Contamination of soil by the activities of exploration, production, and disposal of oil waste into the environment causes serious damage to the environmental ecosystem, the target of processing by the bacteria as a model for remediation of oil contaminated site. Thus, the study was focused on determining the biodegradation percentage of extractable petroleum hydrocarbons as a function of the oil concentration. This research was conducted in a slurry bioreactor with mixed contaminated soil to water ratio of 20:80 (wt.%). A consortium of Bacillus cereus and Pseudomonas putida bacteria 10% (v/v) and 15% (v/v) with the ratio of 2:3, 1:1, and 3:2 was inserted into the slurry bioreactor and a single reactor was used as a control. The result of identification with an initial concentration of extractable petroleum hydrocarbons of 299.53 ng/µL, after 49 days of incubation for bacterial consortium 10% (v/v), the concentration was reduced to 85.31; 32.43; 59.74; and 112.22 ng/µL respectively and the biodegradation percentage was 71.5; 89.17; 80.05; and 62.54%. As for the bacterial consortium concentration of 15% (v/v) with the same ratio and control, the effluent concentration was 12.48; 7.72; 18.93 ng/µL, respectively or the biodegradation percentage was 95.83; 97.42; 93.68%.


2019 ◽  
pp. 101-108
Author(s):  
Lilija Kalediene ◽  
Grazina Giedraityte ◽  
Rapolas Liuzinas

The present study was undertaken to evaluate the efficacy of introduced indigenousbacterial isolates for ex situ bioremediation of fuel oil contaminated soil. For this purposethree hydrocarbon-degrading indigenous bacterial isolates were screened from petroleumoil contaminated soil and repeatedly used for inoculation of fuel oil contaminated soil.The total petroleum hydrocarbons (TPH) content was determined by gravimetric method,Hydrocarbon fractions (alkanes, aromatics, asphaltenes and resins) present in TPH wereobtained by silica gel column chromatography. The study showed that some introducedbacterial isolates effectively adapted to the contaminated soil. The bioaugmentation effectwas calculated to raise the numbers of bacteria by approximately one order of magnitudefrom the indigenous population at the site. Ex situ study showed that the introducedbacterial consortium effectively adapted to the local environment of the soil at thebioremediation site.Our results indicated that disappearance of TPH from inoculated soil samples dependedon the general soil impurity, term of bacterial treatment, level of TPH contamination andindividual microorganism efficacy. With application of bacterial consortium andfertilizers, the TPH level was reduced to 60 - 66% after three months.


2018 ◽  
Vol 40 (18) ◽  
pp. 2345-2353 ◽  
Author(s):  
Chuanhua Wang ◽  
Lingfeng Gu ◽  
Shimei Ge ◽  
Xiaoyan Liu ◽  
Xinying Zhang ◽  
...  

2018 ◽  
Vol 25 (15) ◽  
pp. 14437-14446 ◽  
Author(s):  
Ruiwei Xu ◽  
Zhen Zhang ◽  
Liping Wang ◽  
Ningning Yin ◽  
Xiaohui Zhan

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