scholarly journals Comparative Study on A Novel Pathogen of European Seabass. Diversity of Aeromonas veronii in the Aegean Sea

2019 ◽  
Vol 7 (11) ◽  
pp. 504 ◽  
Author(s):  
Maria Smyrli ◽  
Adriana Triga ◽  
Nancy Dourala ◽  
Panos Varvarigos ◽  
Michael Pavlidis ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Geographic Origin ◽  
Aegean Sea ◽  
Biochemical Properties ◽  
Type Vi Secretion System ◽  
The West ◽  
Genomic Level ◽  
Aeromonas Veronii ◽  
European Seabass ◽  
Severe Pathology

Aeromonas veronii is an emerging pathogen causing severe pathology and mortalities in European seabass aquaculture in the Aegean Sea, Mediterranean. More than 50 strains of the pathogen were characterized biochemically and genetically in order to study the epidemiology of the disease, as well as the phylogeny and virulence of the bacterium. Based on the phenotypic characteristics, the isolates form three groups consisting of: (a) the West Aegean Sea, non-motile, non-pigment-producing strains, (b) the West Aegean Sea, motile, and pigment-producing strains and (c) the East Aegean Sea motile strains that produce minute amounts of pigment. All strains were highly similar at the genomic level; however, the pattern of West/East geographic origin was reflected in biochemical properties, in general genomic level comparison and in the putative virulent factors studied. Type VI secretion system was not detected in the western strains. The outer membrane protein (OMP) profile which contains proteins that are putative antigenic factors, was very similar between strains from the different areas. Although most of the OMPs were detected in all strains with great sequence similarity, diversification according to geographic origin was evident in known antigenic factors such as the maltoporin LamB. A systematic comparative analysis of the strains is presented and discussed in view of the emergence of A. veronii as a significant pathogen for the Mediterranean aquaculture.

scholarly journals Aeromonas veronii Infection Associated with High Morbidity and Mortality in Farmed European Seabass Dicentrarchus labrax in the Aegean Sea, Greece

2017 ◽  
Vol 52 (2) ◽  
pp. 68-81 ◽  
Author(s):  
Maria Smyrli ◽  
Athanasios Prapas ◽  
George Rigos ◽  
Constantina Kokkari ◽  
Michail Pavlidis ◽  
...  
Keyword(s):  
Dicentrarchus Labrax ◽  
Aegean Sea ◽  
Morbidity And Mortality ◽  
High Morbidity ◽  
Aeromonas Veronii ◽  
European Seabass

scholarly journals The Pichia pastoris PER6 gene product is a peroxisomal integral membrane protein essential for peroxisome biogenesis and has sequence similarity to the Zellweger syndrome protein PAF-1.

1996 ◽  
Vol 16 (5) ◽  
pp. 2527-2536 ◽  
Author(s):  
H R Waterham ◽  
Y de Vries ◽  
K A Russel ◽  
W Xie ◽  
M Veenhuis ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Membrane Protein ◽  
Sequence Similarity ◽  
Zellweger Syndrome ◽  
Methylotrophic Yeast ◽  
Integral Membrane Protein ◽  
Biochemical Properties ◽  
Podospora Anserina ◽  
Peroxisome Biogenesis ◽  
Membrane Spanning

We report the cloning of PER6, a gene essential for peroxisome biogenesis in the methylotrophic yeast Pichia pastoris. The PER6 sequence predicts that its product Per6p is a 52-kDa polypeptide with the cysteine-rich C3HC4 motif. Per6p has significant overall sequence similarity with the human peroxisome assembly factor PAF-1, a protein that is defective in certain patients suffering from the peroxisomal disorder Zellweger syndrome, and with car1, a protein required for peroxisome biogenesis and caryogamy in the filamentous fungus Podospora anserina. In addition, the C3HC4 motif and two of the three membrane-spanning segments predicted for Per6p align with the C3HC4 motifs and the two membrane-spanning segments predicted for PAF-1 and car1. Like PAF-1, Per6p is a peroxisomal integral membrane protein. In methanol- or oleic acid-induced cells of per6 mutants, morphologically recognizable peroxisomes are absent. Instead, peroxisomal remnants are observed. In addition, peroxisomal matrix proteins are synthesized but located in the cytosol. The similarities between Per6p and PAF-1 in amino acid sequence and biochemical properties, and between mutants defective in their respective genes, suggest that Per6p is the putative yeast homolog of PAF-1.

scholarly journals Amphibacillus jilinensis sp. nov., a facultatively anaerobic, alkaliphilic bacillus from a soda lake

2010 ◽  
Vol 60 (11) ◽  
pp. 2540-2543 ◽  
Author(s):  
Xiao-Yue Wu ◽  
Gang Zheng ◽  
Wen-Wu Zhang ◽  
Xue-Wei Xu ◽  
Min Wu ◽  
...  
Keyword(s):  
Sequence Similarity ◽  
Soda Lake ◽  
Soluble Starch ◽  
Biochemical Properties ◽  
Phylogenetic Position ◽  
Rrna Gene ◽  
Positive Staining ◽  
Strictly Anaerobic ◽  
Dna Relatedness ◽  
Facultatively Anaerobic

A facultatively anaerobic, alkaliphilic, spore-forming, Gram-positive-staining rod, designated Y1T, was isolated under strictly anaerobic conditions from sediment of a soda lake in Jilin province, China. The strain was not dependent on Na+ but was highly halotolerant and grew optimally in medium JY with 0.5 M Na+ (0.06 M NaHCO3 and 0.44 M NaCl). The optimum pH for growth was 9.0, with a range of pH 7.5–10.5. No growth occurred at pH 7.0 or 11.0. The strain was mesophilic, with a temperature range of 15–45 °C and optimum growth at 32 °C. Strain Y1T was able to use certain mono- and oligosaccharides. Soluble starch and casein were hydrolysed. The methyl red test, Voges–Proskauer test and tests for catalase and oxidase activities were negative. The predominant fatty acids were anteiso-C15 : 0 and iso-C15 : 0. Comparative 16S rRNA gene sequence analysis revealed 93.4–96.8 % sequence similarity to members of the genus Amphibacillus. The DNA G+C content was 37.7 mol% (T m method). The DNA–DNA relatedness of strain Y1T with respect to Amphibacillus tropicus DSM 13870T and Amphibacillus sediminis DSM 21624T was 48 and 37 %, respectively. On the basis of its phylogenetic position and the DNA–DNA relatedness data as well as its physiological and biochemical properties, strain Y1T represents a novel species of the genus Amphibacillus, for which the name Amphibacillus jilinensis sp. nov. is proposed. The type strain is Y1T (=CGMCC 1.5123T =JCM 16149T).

Asanoa siamensis sp. nov., isolated from soil from a temperate peat swamp forest

2013 ◽  
Vol 63 (Pt_1) ◽  
pp. 66-71 ◽  
Author(s):  
Nantawan Niemhom ◽  
Chanwit Suriyachadkun ◽  
Tomohiko Tamura ◽  
Chitti Thawai
Keyword(s):  
Type Species ◽  
Sequence Similarity ◽  
Biochemical Properties ◽  
Diaminopimelic Acid ◽  
Rrna Gene ◽  
Swamp Forest ◽  
Peat Swamp Forest ◽  
Content Type ◽  
Link Type ◽  
Peat Swamp

A novel actinomycete strain, PS7-2T, which produced spore chains borne on the tips of short sporophores, was isolated from soil collected from a temperate peat swamp forest in Phu-Sang National Park, Phayao province, Thailand. The isolate contained glutamic acid, glycine, alanine, 3-hydroxy-diaminopimelic acid and meso-diaminopimelic acid in the cell-wall peptidoglycan. The whole-cell sugars were glucose, mannose, rhamnose and xylose, and the major phospholipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol and phosphatidylethanolamine. The predominant menaquinones were MK-10(H8) and MK-9(H8) and the predominant cellular fatty acids were iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The G+C content of the genomic DNA was 72.3 mol%. On the basis of 16S rRNA gene sequence analysis, strain PS7-2T clustered with members of the genus Asanoa and appeared most closely related to the type strains of Asanoa hainanensis (99.5 % sequence similarity), Asanoa iriomotensis (99.0 %), Asanoa ishikariensis (98.9 %) and Asanoa ferruginea (98.5 %). DNA–DNA hybridizations and some physiological and biochemical properties indicated that strain PS7-2T could be readily distinguished from its closest phylogenetic relatives. Based on the phenotypic and genotypic evidence and DNA–DNA relatedness values, strain PS7-2T represents a novel species in the genus Asanoa , for which the name Asanoa siamensis sp. nov. is proposed; the type strain is PS7-2T ( = BCC 41921T = NBRC 107932T).

scholarly journals A New Outbreak in Sea Bass Farming in Turkey: Aeromonas veronii

2021 ◽  
Vol 72 (3) ◽  
pp. 3051
Author(s):  
T TANSEL TANRIKUL ◽  
E DINÇTÜRK
Keyword(s):  
Clinical Symptoms ◽  
Aegean Sea ◽  
Sea Bass ◽  
Economic Loss ◽  
The Body ◽  
Production Cycle ◽  
Preventive Strategy ◽  
Bacterial Diseases ◽  
Aeromonas Veronii ◽  
Diseased Fish

Turkey produces most of the European sea bassin Europe and bacterial diseases are the main cause of economic loss during the production cycle. This research presents the first extended study of the Aeromonas veronii infection in sea bass on the Turkish coast of the Aegean Sea. An outbreak was observed in three different sea bass farms and diseased fish with clinical symptoms were sampled. Abdominal distention, hemorrhages on the body and anus, enlarged spleen and liver, and hemorrhages in the internal organs were detected from clinical and pathological examinations. Biochemical and molecular identification confirmed the pathogen to be A.veronii.The histopathological observations demonstrated that the pathogen caused bacterial colonies in the fibrous connective tissue, granuloma, and vacuolar degeneration.The primary causes of the disease were proved through an infection experiment. 80% and 90% mortality were calculated in 0.85 x 108 CFU ml-1 and 1.28 x 108 CFU ml-1 experimentally infected groups with clinical and pathological signs of the disease respectively. Recently, the pathological symptoms of the disease had been confused with pasteurellosis infection in cage farms but the presence of A.veronii has been confirmed in the current study. A detailed study is needed to investigate the overall status of the disease in the Aegean Sea in order to design an appropriate preventive strategy.

scholarly journals Polaromonas hydrogenivorans sp. nov., a psychrotolerant hydrogen-oxidizing bacterium from Alaskan soil

2007 ◽  
Vol 57 (3) ◽  
pp. 616-619 ◽  
Author(s):  
Maria Sizova ◽  
Nicolai Panikov
Keyword(s):  
Gene Sequence ◽  
Sequence Similarity ◽  
Biochemical Properties ◽  
Rrna Gene ◽  
Organic Substrates ◽  
The Novel ◽  
Membrane Phospholipids ◽  
Wide Range ◽  
Physiological And Biochemical ◽  
Physiological And Biochemical Properties

Psychrotolerant (0–25 °C), chemolithotrophic Gram-negative cocci were isolated from Alaskan forest soil. The novel isolate was found to grow autotrophically on H2 : CO2 mixtures and to switch to heterotrophic growth on media containing organic substrates. The novel strain utilized a wide range of organic acids, some simple sugars and alcohols. Naphthalene vapour did not support growth. On the basis of 16S rRNA gene sequence similarity, the novel strain is affiliated to the genus Polaromonas, of the class Betaproteobacteria, and is related to Polaromonas naphthalenivorans (99.6 % gene sequence similarity), Polaromonas aquatica (97.4 %) and Polaromonas vacuolata (96.1 %). The membrane phospholipids contained 16 : 1ω7c/16 : 1ω6c, 16 : 0 and 18 : 1ω7c, similar to the fatty acids found for P. naphthalenivorans, P. aquatica and P. vacuolata. On the basis of DNA–DNA hybridization, physiological and biochemical properties, the hydrogen-oxidizing mixotrophic isolate represents a novel species, for which the name Polaromonas hydrogenivorans sp. nov. is proposed. The type strain is DSM 17735T (=NRRL B-41369T).

scholarly journals Oceanisphaera donghaensis sp. nov., a halophilic bacterium from the East Sea, Korea

2006 ◽  
Vol 56 (4) ◽  
pp. 895-898 ◽  
Author(s):  
Soo-Je Park ◽  
Cheol-Hee Kang ◽  
Young-Do Nam ◽  
Jin-Woo Bae ◽  
Yong-Ha Park ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Gene Sequence ◽  
Sequence Similarity ◽  
Halophilic Bacterium ◽  
Biochemical Properties ◽  
East Sea ◽  
16S Rrna Gene Sequence ◽  
Rrna Gene ◽  
Rrna Gene Sequence

A taxonomic study was carried out on two isolates, strains BL1T and BL11, from marine sediment collected from the East Sea, Korea. Comparative 16S rRNA gene sequence studies showed that these isolates clearly affiliated with the Gammaproteobacteria. BL1T and BL11 were most closely related to Oceanisphaera litoralis KMM 3654T (97·6 and 97·7 % 16S rRNA gene sequence similarity, respectively). The level of 16S rRNA gene sequence similarity between strains BL1T and BL11 was 99·7 %. The two isolates were Gram-negative, aerobic, moderately halophilic, and grew in 0·5–8·0 % NaCl and at 4–42 °C. Strains BL1T and BL11 shared some physiological and biochemical properties with O. litoralis KMM 3654T, although they differed in that BL1T and BL11 were able to utilize ethanol, proline and alanine. The G+C contents of the genomic DNA of strains BL1T and BL11 were 56·6 and 57·1 mol%, respectively. Both strains possessed C16 : 1 ω7c and/or iso-C15 : 0 2-OH, C16 : 0 and C18 : 1 ω7c as the major fatty acids. DNA–DNA relatedness data indicated that strains BL1T and BL11 represent a genomic species that is separate from O. litoralis KMM 3654T. On the basis of polyphasic evidence, it is proposed that strain BL1T (=KCTC 12522T=DSM 17589T) represents the type strain of a novel species, Oceanisphaera donghaensis sp. nov.

Morphotectonic analysis along the northern margin of Samos Island, related to the seismic activity of October 2020, Aegean Sea, Greece

2021 ◽  
Author(s):  
Paraskevi Nomikou ◽  
Dimitris Evangelidis ◽  
Dimitris Papanikolaou ◽  
Danai Lampridou ◽  
Dimitris Litsas ◽  
...  
Keyword(s):  
Seismic Activity ◽  
Normal Fault ◽  
Aegean Sea ◽  
The South ◽  
The West ◽  
Northern Margin ◽  
Hydrographic Survey ◽  
Southern Margin ◽  
Eastern Aegean ◽  
Division Line

<p>On October 30<sup>th</sup> 2020 a strong earthquake of magnitude 7.0 occurred north of Samos Island at the Eastern Aegean Sea. This seismic event was another destructive active deformation in the long seismic history of Samos since the ancient times. Preliminary reports focused the seismic epicenter at about 10 km north of Karlovassi, situated at the western part of the Samos E-W trending coastline. The earthquake mechanism corresponds to an E-W normal fault dipping to the north. The activated fault was assumed to be running along the northern margin of Samos Island, which bounds from the south the Samos basin.</p><p>Immediately after the seismic activity and during the aftershock period in December 2020 an hydrographic survey off the northern coastal margin of Samos Island was conducted with R/V NAUTILOS of the Hellenic Navy Hydrographic Service, using the multibeam SeaBat 7160 RESON. The result of the hydrographic survey was a detailed bathymetric map with 15m grid interval and 50m isobaths.  The main morphological aspects of Samos Basin are a 14 km long, 6 km wide and 690 m deep elongated E-W basin developed north of Samos Island.</p><p>The southern margin of the basin is abrupt with morphological slopes of more than 10<sup>o</sup>, following the major E-W normal fault surface, running along the coastal zone, with an overall throw of more than 500m. In contrast, the northern margin of the basin shows a gradual slope increase towards the south from 1<sup>o</sup> to 5<sup>o</sup>. Numerous small canyons trending N-S transversal to the main direction of the Samos coastline are observed along the southern margin, between 600 and 100 m water depth.  These canyons have a length around 2,7 km and width between 100-300 m. Two large submarine landslides with a canyon width of 1,3 km and 0,8 Km, are located north of Karlovasi. The creation of the canyons is probably due to the uplift of Northern Samos Island and their 500 m vertical height difference corresponds to the average fault throw that has controlled the steep slopes of the margin. The orientation of the fault scarp changes at the western Samos coastline from E-W to ENE-WSW facing the neighboring Ikaria Basin, which is developed to the west of Samos Basin. The division line between the Ikaria and Samos basins runs N-S from the northern slopes and coast of the Kerketeas mountain (1443m). The aftershocks of the 30<sup>th</sup> October main shock are limited east of the N-S division line with only a minor activity 15 km to the west within the eastern margin of the Ikaria Basin.</p>

scholarly journals Characterization of the KLP68D kinesin-like protein in Drosophila: possible roles in axonal transport.

1994 ◽  
Vol 127 (4) ◽  
pp. 1041-1048 ◽  
Author(s):  
P A Pesavento ◽  
R J Stewart ◽  
L S Goldstein
Keyword(s):  
Nervous System ◽  
Axonal Transport ◽  
Heavy Chain ◽  
Sequence Similarity ◽  
Coiled Coil ◽  
Biochemical Properties ◽  
Motor Domain ◽  
Tail Region ◽  
Anterograde Axonal Transport ◽  
Kinesin Heavy Chain

This paper describes the molecular and biochemical properties of KLP68D, a new kinesin-like motor protein in Drosophila melanogaster. Sequence analysis of a full-length cDNA encoding KLP68D demonstrates that this protein has a domain that shares significant sequence identity with the entire 340-amin acid kinesin heavy chain motor domain. Sequences extending beyond the motor domain predict a region of alpha-helical coiled-coil followed by a globular "tail" region; there is significant sequence similarity between the alpha-helical coiled-coil region of the KLP68D protein and similar regions of the KIF3 protein of mouse and the KRP85 protein of sea urchin. This finding suggests that all three proteins may be members of the same family, and that they all perform related functions. KLP68D protein produced in Escherichia coli is, like kinesin itself, a plus-end directed microtubule motor. In situ hybridization analysis of KLP68D RNA in Drosophila embryos indicates that the KLP68D gene is expressed primarily in the central nervous system and in a subset of the peripheral nervous system during embryogenesis. Thus, KLP68D may be used for anterograde axonal transport and could conceivably move cargoes in fly neurons different than those moved by kinesin heavy chain or other plus-end directed motors.

Sphingomonas yabuuchiae sp. nov. and Brevundimonas nasdae sp. nov., isolated from the Russian space laboratory Mir

2004 ◽  
Vol 54 (3) ◽  
pp. 819-825 ◽  
Author(s):  
Ying Li ◽  
Yoshiaki Kawamura ◽  
Nagatoshi Fujiwara ◽  
Takashi Naka ◽  
Hongsheng Liu ◽  
...  
Keyword(s):  
Sequence Analysis ◽  
16S Rdna ◽  
Related Species ◽  
Type Strain ◽  
Novel Species ◽  
Sequence Similarity ◽  
Biochemical Properties ◽  
Rdna Sequence ◽  
Rrna Gene ◽  
16S Rdna Sequence

On the basis of phenotypic and genotypic characteristics and 16S rRNA gene sequence analysis, novel species belonging to the genera Sphingomonas and Brevundimonas were identified from samples taken from the Russian space laboratory Mir. Strain A1-18T was isolated from the air. 16S rDNA sequence analysis showed that strain A1-18T formed a coherent cluster with Sphingomonas sanguinis, Sphingomonas parapaucimobilis, Sphingomonas paucimobilis and Sphingomonas roseiflava with sequence similarity of 97·5–98·6 %. Similar to other Sphingomonas species, the G+C content was 66·1 mol%, but DNA–DNA hybridization rates at optimal temperatures among these related species were only 24·7–51·7 %. Strain A1-18T can be differentiated biochemically from related species. Strain W1-2BT was isolated from condensation water. It forms a distinct lineage within the genus Brevundimonas, forming a coherent cluster with Brevundimonas vesicularis, Brevundimonas aurantiaca and Brevundimonas intermedia. 16S rDNA sequence similarities were 98·6–99·5 % and the G+C content was 66·5 mol%, similar to other Brevundimonas species, but DNA–DNA relatedness was only 50·2–54·8 %. Strain W1-2BT also showed some differential biochemical properties from its related species. A series of polyphasic taxonomic studies led to the proposal of two novel species, Sphingomonas yabuuchiae sp. nov. (type strain A1-18T=GTC 868T=JCM 11416T=DSM 14562T) and Brevundimonas nasdae sp. nov. (type strain W1-2BT=GTC 1043T=JCM 11415T=DSM 14572T).

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