scholarly journals Accelerating the Biodegradation of High-Density Polyethylene (HDPE) Using Bjerkandera adusta TBB-03 and Lignocellulose Substrates

2019 ◽  
Vol 7 (9) ◽  
pp. 304 ◽  
Author(s):  
Bo Ram Kang ◽  
Soo Bin Kim ◽  
Hyun A Song ◽  
Tae Kwon Lee
Keyword(s):  
High Density Polyethylene ◽  
Amorphous Structure ◽  
High Density ◽  
Wood Chips ◽  
White Rot ◽  
Organic Polymer ◽  
White Rot Fungus ◽  
Laccase Production ◽  
Bjerkandera Adusta ◽  
Emerging Pollutant

High-density polyethylene (HDPE) is a widely used organic polymer and an emerging pollutant, because it is very stable and nonbiodegradable. Several fungal species that produce delignifying enzymes are known to be promising degraders of recalcitrant polymers, but research on the decomposition of plastics is scarce. In this study, white rot fungus, Bjerkandera adusta TBB-03, was isolated and characterized for its ability to degrade HDPE under lignocellulose substrate treatment. Ash (Fraxinus rhynchophylla) wood chips were found to stimulate laccase production (activity was > 210 U/L after 10 days of cultivation), and subsequently used for HDPE degradation assay. After 90 days, cracks formed on the surface of HDPE samples treated with TBB-03 and ash wood chips in both liquid and solid states. Raman analysis showed that the amorphous structure of HDPE was degraded by enzymes produced by TBB-03. Overall, TBB-03 is a promising resource for the biodegradation of HDPE, and this work sheds light on further applications for fungus-based plastic degradation systems.

Chlorometabolite production by the ecologically important white rot fungus Bjerkandera adusta

Chemosphere ◽  
2001 ◽  
Vol 44 (7) ◽  
pp. 1603-1616 ◽  
Author(s):  
P.J Silk ◽  
C Aubry ◽  
G.C Lonergan ◽  
J.B Macaulay
Keyword(s):  
White Rot ◽  
White Rot Fungus ◽  
Bjerkandera Adusta

scholarly journals Recalcitrant Compounds Removal in Raw Leachate and Synthetic Effluents Using the White-Rot Fungus Bjerkandera adusta

Water ◽  
2017 ◽  
Vol 9 (11) ◽  
pp. 824 ◽  
Author(s):  
Alessandra Bardi ◽  
Qiuyan Yuan ◽  
Valeria Tigini ◽  
Federica Spina ◽  
Giovanna Varese ◽  
...  
Keyword(s):  
White Rot ◽  
White Rot Fungus ◽  
Bjerkandera Adusta ◽  
Recalcitrant Compounds

Alkaline-sulfite chemithermomechanical pulping of Eucalyptus grandis biotreated by Ceriporiopsis subvermispora under varied culture conditions

Holzforschung ◽  
2008 ◽  
Vol 62 (4) ◽  
Author(s):  
Marcos Paulo Vicentim ◽  
André Ferraz
Keyword(s):  
Fiber Length ◽  
Corn Steep Liquor ◽  
Eucalyptus Grandis ◽  
Culture Conditions ◽  
Wood Chips ◽  
White Rot ◽  
White Rot Fungus ◽  
Ceriporiopsis Subvermispora ◽  
Fungal Pretreatment ◽  
Chemithermomechanical Pulping

Abstract The effect of different culture conditions have been evaluated concerning the extracellular enzyme activities of the white-rot fungus Ceriporiopsis subvermispora growing on Eucalyptus grandis wood. The consequence of the varied fungal pretreatment on a subsequent chemithermomechanical pulping (CTMP) was addressed. In all cultures, manganese peroxidase (MnP) and xylanase were the predominant extracellular enzymes. The biopulping efficiency was evaluated based on the amount of fiber bundles obtained after the first fiberizing step and the fibrillation levels of refined pulps. It was found that the MnP levels in the cultures correlated positively with the biopulping benefits. On the other hand, xylanase and total oxalate levels did not vary significantly. Accordingly, it was not possible to determine whether MnP accomplishes the effect alone or depends on synergic action of other extracellular agents. Pulp strength and fiber size distribution were also evaluated. The average fiber length of CTMP pulps prepared from untreated wood chips was 623 μm. Analogous values were observed for most of the biopulps; however, significant amounts of shorter fibers were found in the biopulp prepared from wood chips biotreated in cultures supplemented with glucose plus corn-steep liquor. Despite evidence of reduced average fiber length, biopulps prepared from these wood chips presented the highest improvement in tensile indexes (+28% at 23° Schopper-Riegler).

scholarly journals Interdependence of Primary Metabolism and Xenobiotic Mitigation Characterizes the Proteome of Bjerkandera adusta during Wood Decomposition

2017 ◽  
Vol 84 (2) ◽  
Author(s):  
S. C. Moody ◽  
E. Dudley ◽  
J. Hiscox ◽  
L. Boddy ◽  
D. C. Eastwood
Keyword(s):  
Incubation Temperature ◽  
Protein Profile ◽  
Nutrient Acquisition ◽  
Protein Domain ◽  
White Rot ◽  
White Rot Fungus ◽  
Primary Metabolism ◽  
Bjerkandera Adusta ◽  
Content Type ◽  
Key Features

ABSTRACTThe aim of the current work was to identify key features of the fungal proteome involved in the active decay of beechwood blocks by the white rot fungusBjerkandera adustaat 20°C and 24°C. A combination of protein and domain analyses ensured a high level of annotation, which revealed that while the variation in the proteins identified was high between replicates, there was a considerable degree of functional conservation between the two temperatures. Further analysis revealed differences in the pathways and processes employed by the fungus at the different temperatures, particularly in relation to nutrient acquisition and xenobiotic mitigation. Key features showing temperature-dependent variation in mechanisms for both lignocellulose decomposition and sugar utilization were found, alongside differences in the enzymes involved in mitigation against damage caused by toxic phenolic compounds and oxidative stress.IMPORTANCEThis work was conducted using the wood decay fungusB. adusta, grown on solid wood blocks to closely mimic the natural environment, and gives greater insight into the proteome of an important environmental fungus during active decay. We show that a change in incubation temperature from 20°C to 24°C altered the protein profile. Proteomic studies in the field of white-rotting basidiomycetes have thus far been hampered by poor annotation of protein databases, with a large proportion of proteins simply with unknown function. This study was enhanced by extensive protein domain analysis, enabling a higher level of functional assignment and greater understanding of the proteome composition. This work revealed a strong interdependence of the primary process of nutrient acquisition and specialized metabolic processes for the detoxification of plant extractives and the phenolic breakdown products of lignocellulose.

scholarly journals Enhancing laccase production by white-rot fungus Funalia floccosa LPSC 232 in co-culture with Penicillium commune GHAIE86

2018 ◽  
Vol 64 (1) ◽  
pp. 91-99 ◽  
Author(s):  
Rosario Díaz Rodríguez ◽  
Gabriela Heredia ◽  
José A. Siles ◽  
Miguel Jurado ◽  
Mario Carlos Nazareno Saparrat ◽  
...  
Keyword(s):  
White Rot ◽  
White Rot Fungus ◽  
Laccase Production ◽  
Penicillium Commune

Bioremediation of a wine distillery wastewater using white rot fungi and the subsequent production of laccase

2007 ◽  
Vol 56 (2) ◽  
pp. 179-186 ◽  
Author(s):  
P.J. Strong ◽  
J.E. Burgess
Keyword(s):  
Biological Treatment ◽  
Oxygen Demand ◽  
White Rot Fungi ◽  
White Rot ◽  
White Rot Fungus ◽  
Laccase Production ◽  
Total Phenolic ◽  
Pycnoporus Cinnabarinus ◽  
Distillery Wastewater

The aim of this work was to ascertain whether a submerged culture of a white rot fungus could be used to treat distillery wastewater, and whether the compounds present in the wastewater would stimulate laccase production. Trametes pubescens MB 89, Ceriporiopsissubvermispora, Pycnoporus cinnabarinus and UD4 were screened for their ability for the bioremediation of a raw, untreated distillery wastewater as well as distillery wastewater that had been pretreated by polyvinylpolypyrrolidone. Suitability of each strain was measured as a function of decreasing the chemical oxygen demand (COD) and total phenolic compounds concentration and the colour of the wastewater, while simultaneously producing laccase in high titres. After screening, T. pubescens MB 89 was used further in flask cultures and attained 79±1.1% COD removal, 80±4.6% total phenols removal, 71±1.6% decrease in colour at an absorbance of 500 nm and increased the pH from 5.3 to near-neutral. Laccase activity in flask cultures peaked at 4,644±228 units/l, while the activity in a 50 l bubble lift reactor peaked at 12,966±71 units/l. Trametes pubescens MB 89 greatly improved the quality of a wastewater known for toxicity towards biological treatment systems, while simultaneously producing an industrially relevant enzyme.

An extracellular aryl-alcohol oxidase from the white-rot fungus Bjerkandera adusta

1990 ◽  
Vol 12 (3) ◽  
pp. 204-209 ◽  
Author(s):  
Andreas Muheim ◽  
Roland Waldner ◽  
Matti S.A. Leisola ◽  
Armin Fiechter
Keyword(s):  
Alcohol Oxidase ◽  
White Rot ◽  
White Rot Fungus ◽  
Bjerkandera Adusta
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