scholarly journals Preparation of an Acridinium Ester-Labeled Antibody and Its Application in GoldMag Nanoparticle-Based, Ultrasensitive Chemiluminescence Immunoassay for the Detection of Human Epididymis Protein 4

Micromachines ◽  
2017 ◽  
Vol 8 (5) ◽  
pp. 149 ◽  
Author(s):  
Ting Ma ◽  
Mengdan Zhang ◽  
Yinsheng Wan ◽  
Yali Cui ◽  
Le Ma
Keyword(s):  
Chemiluminescence Immunoassay ◽  
Human Epididymis Protein 4 ◽  
Human Epididymis ◽  
Acridinium Ester

Relation between Human Epididymis Protein 4 and Endometrial Pathology in Women with Postmenopausal Bleeding

2020 ◽  
Vol 11 (1) ◽  
pp. 937
Author(s):  
Ahmed L. Aboul Nasr ◽  
Ghada A. Abdel Moety ◽  
Mostafa S. Salem ◽  
Marwa M. Elsharkawy ◽  
Nada Kamal ◽  
...  
Keyword(s):  
Postmenopausal Bleeding ◽  
Human Epididymis Protein 4 ◽  
Human Epididymis ◽  
Endometrial Pathology

Human Epididymis Protein 4 Quantification and Interaction Network Analysis in Seminal Plasma

2019 ◽  
Vol 26 (6) ◽  
pp. 458-465
Author(s):  
Krishna Kant ◽  
Anil K. Tomar ◽  
Pankaj Sharma ◽  
Bishwajit Kundu ◽  
Sarman Singh ◽  
...  
Keyword(s):  
Seminal Plasma ◽  
Binding Proteins ◽  
Interaction Network ◽  
Prostatic Acid Phosphatase ◽  
Regulatory Subunit ◽  
Plasma Samples ◽  
Human Seminal Plasma ◽  
Human Epididymis Protein 4 ◽  
Human Epididymis ◽  
Core Proteins

Background: A well-known tissue marker of ovarian cancer, Human Epididymis protein 4 (HE4) is the member of whey acidic four-disulfide core proteins family. Purified from human seminal plasma and characterized as a cross-class protease inhibitor, HE4 was proposed to shield spermatozoa against proteolytic factors. However, its exact biological function is unknown. Proteins usually function in conjunction with other proteins in the system and thus, identification and analysis of protein networks become essential to decode protein functions. Objective: This study was performed to explore possible role(s) of HE4 in reproductive physiology via identification of its interactome in human seminal plasma. Methods: HE4 binding proteins were identified through co-immunoprecipitation and MALDITOF/ MS analysis. Also, HE4 was quantified by ELISA in fertile and infertile human seminal plasma samples. Results: Ten HE4 binding proteins were identified, viz. protein phosphatase 1 regulatory subunit 21, protein kinase CLK3, Ankyrin repeat domain-containing protein36A, prostatic acid phosphatase, KIF5C, Spectrin repeat containing, nuclear envelope 1, isoform CRAf, tropomyosin 4, vezatin, utrophin and fibronectin1. This interaction network suggests that HE4 plays multiple roles, specifically in capacitation, sperm motility and maturation. Further, HE4 concentration in human seminal plasma samples was determined by Elisa. Higher HE4 expression in normozoospermia compared to azoospermia and asthenozoospermia affirms its importance in fertilization. Conclusion: Based on identified interactome, it is plausible that HE4 plays a crucial role in fertilization, specifically in sperm maturation, motility and capacitation.

Ultrasensitive chemiluminescence immunoassay with enhanced precision for the detection of cTnI amplified by acridinium ester-loaded microspheres and internally calibrated by magnetic fluorescent nanoparticles

Nanoscale ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 3275-3284
Author(s):  
Huan Zhao ◽  
Qifeng Lin ◽  
Li Huang ◽  
Yunfeng Zhai ◽  
Yuan Liu ◽  
...  
Keyword(s):  
Human Body ◽  
Accurate Diagnosis ◽  
Fluorescent Nanoparticles ◽  
Internal Standards ◽  
Chemiluminescence Immunoassay ◽  
Potential Signal ◽  
Acridinium Ester ◽  
Hydrogel Microspheres

Hydrogel microspheres sensitive to temperature as new potential signal enhancers and magnetic fluorescent nanoparticles as internal standards were used to establish a new CLIA method for the accurate diagnosis of cTnI in the human body.

scholarly journals Clinical Usefulness of Human Epididymis Protein 4 in Lung Cancer

2017 ◽  
Vol 37 (6) ◽  
pp. 526-530 ◽  
Author(s):  
Soo In Choi ◽  
Mi-Ae Jang ◽  
Byung Ryul Jeon ◽  
Hee Bong Shin ◽  
You Kyoung Lee ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Clinical Usefulness ◽  
Human Epididymis Protein 4 ◽  
Human Epididymis

Predictive value of serum human epididymis protein 4 and cancer antigen 125 concentrations in endometrial carcinoma

2013 ◽  
Vol 209 (2) ◽  
pp. 142.e1-142.e6 ◽  
Author(s):  
Sami K. Saarelainen ◽  
Nina Peltonen ◽  
Terho Lehtimäki ◽  
Antti Perheentupa ◽  
Maarit H. Vuento ◽  
...  
Keyword(s):  
Endometrial Carcinoma ◽  
Predictive Value ◽  
Cancer Antigen 125 ◽  
Cancer Antigen ◽  
Human Epididymis Protein 4 ◽  
Human Epididymis
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