scholarly journals Fluid Flow Shear Stress Stimulation on a Multiplex Microfluidic Device for Rat Bone Marrow Stromal Cell Differentiation Enhancement

Micromachines ◽  
2015 ◽  
Vol 6 (12) ◽  
pp. 1996-2009 ◽  
Author(s):  
Chia-Wen Tsao ◽  
Yu-Che Cheng ◽  
Jhih-Hao Cheng
Keyword(s):  
Bone Marrow ◽  
Shear Stress ◽  
Fluid Flow ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Flow Shear ◽  
Marrow Stromal Cell ◽  
Flow Shear Stress ◽  
Fluid Flow Shear Stress ◽  
Rat Bone

scholarly journals The Effect of Fluid Flow Shear Stress and Substrate Stiffness on Yes-Associated Protein (YAP) Activity and Osteogenesis in Murine Osteosarcoma Cells

Cancers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 3128
Author(s):  
Thomas R. Coughlin ◽  
Ali Sana ◽  
Kevin Voss ◽  
Abhilash Gadi ◽  
Upal Basu-Roy ◽  
...  
Keyword(s):  
Shear Stress ◽  
Fluid Flow ◽  
Bone Cells ◽  
Bone Cancer ◽  
Substrate Stiffness ◽  
Flow Shear ◽  
Fluid Movement ◽  
Flow Shear Stress ◽  
Fluid Flow Shear Stress ◽  
New Treatments

Osteosarcoma (OS) is an aggressive bone cancer originating in the mesenchymal lineage. Prognosis for metastatic disease is poor, with a mortality rate of approximately 40%; OS is an aggressive disease for which new treatments are needed. All bone cells are sensitive to their mechanical/physical surroundings and changes in these surroundings can affect their behavior. However, it is not well understood how OS cells specifically respond to fluid movement, or substrate stiffness—two stimuli of relevance in the tumor microenvironment. We used cells from spontaneous OS tumors in a mouse engineered to have a bone-specific knockout of pRb-1 and p53 in the osteoblast lineage. We silenced Sox2 (which regulates YAP) and tested the effect of fluid flow shear stress (FFSS) and substrate stiffness on YAP expression/activity—which was significantly reduced by loss of Sox2, but that effect was reversed by FFSS but not by substrate stiffness. Osteogenic gene expression was also reduced in the absence of Sox2 but again this was reversed by FFSS and remained largely unaffected by substrate stiffness. Thus we described the effect of two distinct stimuli on the mechanosensory and osteogenic profiles of OS cells. Taken together, these data suggest that modulation of fluid movement through, or stiffness levels within, OS tumors could represent a novel consideration in the development of new treatments to prevent their progression.

Stimulatory effect of ipriflavone on formation of bone-like tissue in rat bone marrow stromal cell culture

1992 ◽  
Vol 51 (S1) ◽  
pp. S16-S20 ◽  
Author(s):  
Kohei Notoya ◽  
Ryoichi Tsukuda ◽  
Keiji Yoshida ◽  
Shigehisa Taketomi
Keyword(s):  
Cell Culture ◽  
Bone Marrow ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Marrow Stromal Cell ◽  
Stromal Cell Culture ◽  
Rat Bone
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