scholarly journals Macroporous hydroxyapatite scaffolds for bone tissue engineering applications: Physicochemical characterization and assessment of rat bone marrow stromal cell viability

2009 ◽  
Vol 91A (1) ◽  
pp. 175-186 ◽  
Author(s):  
Joaquim M. Oliveira ◽  
Simone S. Silva ◽  
Patricia B. Malafaya ◽  
Marcia T. Rodrigues ◽  
Noriko Kotobuki ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Marrow ◽  
Cell Viability ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Physicochemical Characterization ◽  
Marrow Stromal Cell ◽  
Rat Bone

Stimulatory effect of ipriflavone on formation of bone-like tissue in rat bone marrow stromal cell culture

1992 ◽  
Vol 51 (S1) ◽  
pp. S16-S20 ◽  
Author(s):  
Kohei Notoya ◽  
Ryoichi Tsukuda ◽  
Keiji Yoshida ◽  
Shigehisa Taketomi
Keyword(s):  
Cell Culture ◽  
Bone Marrow ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Marrow Stromal Cell ◽  
Stromal Cell Culture ◽  
Rat Bone

scholarly journals Encapsulation of Rat Bone Marrow Derived Mesenchymal Stem Cells in Alginate Dialdehyde/Gelatin Microbeads with and without Nanoscaled Bioactive Glass for In Vivo Bone Tissue Engineering

Materials ◽  
2018 ◽  
Vol 11 (10) ◽  
pp. 1880 ◽  
Author(s):  
Ulrike Rottensteiner-Brandl ◽  
Rainer Detsch ◽  
Bapi Sarker ◽  
Lara Lingens ◽  
Katrin Köhn ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Marrow ◽  
Cell Survival ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Immune Reaction ◽  
Three Dimensional ◽  
High Cell ◽  
Rat Bone

Alginate dialdehyde (ADA), gelatin, and nano-scaled bioactive glass (nBG) particles are being currently investigated for their potential use as three-dimensional scaffolding materials for bone tissue engineering. ADA and gelatin provide a three-dimensional scaffold with properties supporting cell adhesion and proliferation. Combined with nanocristalline BG, this composition closely mimics the mineral phase of bone. In the present study, rat bone marrow derived mesenchymal stem cells (MSCs), commonly used as an osteogenic cell source, were evaluated after encapsulation into ADA-gelatin hydrogel with and without nBG. High cell survival was found in vitro for up to 28 days with or without addition of nBG assessed by calcein staining, proving the cell-friendly encapsulation process. After subcutaneous implantation into rats, survival was assessed by DAPI/TUNEL fluorescence staining. Hematoxylin-eosin staining and immunohistochemical staining for the macrophage marker ED1 (CD68) and the endothelial cell marker lectin were used to evaluate immune reaction and vascularization. After in vivo implantation, high cell survival was found after 1 week, with a notable decrease after 4 weeks. Immune reaction was very mild, proving the biocompatibility of the material. Angiogenesis in implanted constructs was significantly improved by cell encapsulation, compared to cell-free beads, as the implanted MSCs were able to attract endothelial cells. Constructs with nBG showed higher numbers of vital MSCs and lectin positive endothelial cells, thus showing a higher degree of angiogenesis, although this difference was not significant. These results support the use of ADA/gelatin/nBG as a scaffold and of MSCs as a source of osteogenic cells for bone tissue engineering. Future studies should however improve long term cell survival and focus on differentiation potential of encapsulated cells in vivo.

Rat Bone Marrow Stromal Cell–Conditioned Medium Promotes Early Osseointegration of Titanium Implants

2013 ◽  
Vol 28 (5) ◽  
pp. 1360-1369 ◽  
Author(s):  
Shuhei Tsuchiya ◽  
Kenji Hara ◽  
Masayuki Ikeno ◽  
Yasuhiro Okamoto ◽  
Hideharu Hibi ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Conditioned Medium ◽  
Stromal Cell ◽  
Bone Marrow Stromal Cell ◽  
Titanium Implants ◽  
Marrow Stromal Cell ◽  
Cell Conditioned Medium ◽  
Rat Bone
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