scholarly journals SMILE Platform: An Innovative Microfluidic Approach for On-Chip Sample Manipulation and Analysis in Oral Cancer Diagnosis

Micromachines ◽  
2021 ◽  
Vol 12 (8) ◽  
pp. 885
Author(s):  
Sofia Zoupanou ◽  
Annalisa Volpe ◽  
Elisabetta Primiceri ◽  
Caterina Gaudiuso ◽  
Antonio Ancona ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Low Cost ◽  
High Sensitivity ◽  
Ease Of Use ◽  
Early Screening ◽  
Chip Sample ◽  
Large Audience ◽  
On Chip ◽  
Sensitivity Specificity ◽  
Large Diffusion

Oral cancer belongs to the group of head and neck cancers, and, despite its large diffusion, it suffers from low consideration in terms of prevention and early diagnosis. The main objective of the SMILE platform is the development of a low-cost device for oral cancer early screening with features of high sensitivity, specificity, and ease of use, with the aim of reaching a large audience of possible users and realizing real prevention of the disease. To achieve this goal, we realized two microfluidic devices exploiting low-cost materials and processes. They can be used in combination or alone to obtain on-chip sample preparation and/or detection of circulating tumor cells, selected as biomarkers of oral cancer. The realized devices are completely transparent with plug-and-play features, obtained thanks to a highly customized architecture which enables users to easily use them, with potential for a common use among physicians or dentists with minimal training.

scholarly journals Designing Efficient Low-Cost Paper-Based Sensing Plasmonic Nanoplatforms

Sensors ◽  
2018 ◽  
Vol 18 (9) ◽  
pp. 3035 ◽  
Author(s):  
Laurentiu Susu ◽  
Andreea Campu ◽  
Ana Craciun ◽  
Adriana Vulpoi ◽  
Simion Astilean ◽  
...  
Keyword(s):  
Limit Of Detection ◽  
Low Cost ◽  
High Sensitivity ◽  
Dark Field ◽  
Ease Of Use ◽  
Localized Surface Plasmon ◽  
Surface Enhanced Raman ◽  
Resonance Surface ◽  
Sensitivity Specificity ◽  
Plasmonic Paper

Paper-based platforms can be a promising choice as portable sensors due to their low-cost and facile fabrication, ease of use, high sensitivity, specificity and flexibility. By combining the qualities of these 3D platforms with the optical properties of gold nanoparticles, it is possible to create efficient nanodevices with desired biosensing functionalities. In this work, we propose a new plasmonic paper-based dual localized surface plasmon resonance–surface-enhanced Raman scattering (LSPR-SERS) nanoplatform with improved detection abilities in terms of high sensitivity, uniformity and reproducibility. Specifically, colloidal gold nanorods (GNRs) with a well-controlled plasmonic response were firstly synthesized and validated as efficient dual LSPR-SERS nanosensors in solution using the p-aminothiophenol (p-ATP) analyte. GNRs were then efficiently immobilized onto the paper via the immersion approach, thus obtaining plasmonic nanoplatforms with a modulated LSPR response. The successful deposition of the nanoparticles onto the cellulose fibers was confirmed by LSPR measurements, which demonstrate the preserved plasmonic response after immobilization, as well as by dark-field microscopy and scanning electron microscopy investigations, which confirm their uniform distribution. Finally, a limit of detection for p-ATP as low as 10−12 M has been achieved by our developed SERS-based paper nanoplatform, proving that our optimized plasmonic paper-based biosensing design could be further considered as an excellent candidate for miniaturized biomedical applications.

Smartphone integrated optoelectrowetting (SiOEW) for on-chip sample processing and microscopic detection of water quality

Lab on a Chip ◽  
2018 ◽  
Vol 18 (3) ◽  
pp. 532-539 ◽  
Author(s):  
Dongyue Jiang ◽  
Seunguk Lee ◽  
Sung Woo Bae ◽  
Sung-Yong Park
Keyword(s):  
Water Quality ◽  
Sample Preparation ◽  
Low Cost ◽  
Sample Processing ◽  
Microscopic Detection ◽  
Chip Sample ◽  
On Chip

We present a smartphone integrated optoelectrowetting (SiOEW) device as a low-cost, portable tool for on-chip sample preparation and microscopic detection of water quality.

Innovative 3D Structures Utilizing Wafer Level Fan-Out (WLFO) Technology

2013 ◽  
Vol 2013 (DPC) ◽  
pp. 001486-001519
Author(s):  
Curtis Zwenger ◽  
JinYoung Khim ◽  
YoonJoo Khim ◽  
SeWoong Cha ◽  
SeungJae Lee ◽  
...  
Keyword(s):  
Low Cost ◽  
Ease Of Use ◽  
Wafer Level ◽  
3D Structures ◽  
Wafer Level Packaging ◽  
Functional Convergence ◽  
3D Packaging ◽  
Improved Design ◽  
On Chip ◽  
Mobile Handset

The tremendous growth in the mobile handset, tablet, and networking markets has been fueled by consumer demand for increased mobility, functionality, and ease of use. This, in turn, has been driving an increase in functional convergence and 3D integration of IC devices, resulting in the need for more complex and sophisticated packaging techniques. A variety of advanced IC interconnect technologies are addressing this growing need, such as Thru Silicon Via (TSV), Chip-on Chip (CoC), and Package-on-Package (PoP). In particular, the emerging Wafer Level Fan-Out (WLFO) technology provides unique and innovative extensions into the 3D packaging realm. Wafer Level Fan-Out is a package technology designed to provide increased I/O density within a reduced footprint and profile for low density single & multi-die applications at a lower cost. The improved design capability of WLFO is due, in part, to the fine feature capabilities associated with wafer level packaging. This can allow much more aggressive design rules to be applied compared to competing laminate-based technologies. In addition, the unique characteristics of WLFO enable innovative 3D structures to be created that address the need for IC integration in emerging mobile and networking applications. This paper will review the development of WLFO and its extension into unique 3D structures. In addition, the advantages of these WLFO designs will be reviewed in comparison to current competing packaging technologies. Process & material characterization, design simulation, and reliability data will be presented to show how WLFO is poised to provide robust, reliable, and low cost 3D packaging solutions for advanced mobile and networking products.

scholarly journals New traps for the capture of Aedes aegypti (Linnaeus) and Aedes albopictus (Skuse) (Diptera: Culicidae) eggs and adults

2020 ◽  
Author(s):  
Karina Rossi da Silva ◽  
William Ribeiro da Silva ◽  
Bianca Piraccini Silva ◽  
Adriano Nobre Arcos ◽  
Francisco A. da Silva Ferreira ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Aedes Albopictus ◽  
High Efficiency ◽  
Low Cost ◽  
High Sensitivity ◽  
Ease Of Use ◽  
Monitoring And Control ◽  
Density Index ◽  
Egg Density ◽  
And Control

AbstractThe control of arboviruses carried by Aedes aegypti (Linnaeus) and Aedes albopictus (Skuse) can be performed with tools that monitor and reduce the circulation of these vectors. Therefore, the efficiency of four types of traps in capturing A. aegypti and A. albopictus eggs and adults, with the biological product Vectobac WG®, was evaluated in the field. For this, 20 traps were installed in two locations, which were in the South (Londrina, Paraná) and North (Manaus, Amazonas) Regions of Brazil, from March to April 2017 and January to February 2018, respectively. The UELtrap-E and UELtrap-EA traps captured A. aegypti and A. albopictus eggs: 1703/1866 eggs in Londrina, and 10268/2149 eggs in Manaus, respectively, and presented high ovitraps positivity index (OPI) values (averages: 100%/100% in Londrina, and 100%/96% in Manaus, respectively); and high egg density index (EDI) values (averages: 68/75 in Londrina, and 411/89 in Manaus, respectively), so they had statistically superior efficiency to that of the CRtrap-E and CRtrap-EA traps in both regions, that captured less eggs and adults: 96/69 eggs in Londrina, and 1091/510 eggs in Manaus, respectively. Also presented lower OPI values (averages: 28%/4% in Londrina, and 88%/60% in Manaus, respectively); and lower EDI values (averages: 10.5/9 in Londrina, and 47/30 in Manaus, respectively). The capture ratios of Aedes adults in the UELtrap-EA and CRtrap-EA traps in Londrina and Manaus were 53.3%/29.5% and 0%/9.8%, respectively. UELtrap-E and UELtrap-EA can be adopted as efficient tools for Aedes monitoring due to their high sensitivity, low cost and ease of use.Author summaryAedes aegypti and Aedes albopictus are species of mosquitoes responsible for the transmission of several arboviruses that cause infections worldwide. However, there are still no effective and safe vaccines or medications to prevent or treat arboviruses transmitted by these vectors, except for yellow fever. Moreover, current methodologies for monitoring and controlling A. aegypti and A. albopictus are not fully effective, as evidenced by the increasing cases of the arbovirus transmitted by these mosquitoes or have incompatible costs with the socioeconomic conditions of a large number of people. Thus, the traps tested in this study can be used as more effective and economical tools for monitoring and controlling A. aegypti and A. albopictus, since they are made with low cost material and they showed high efficiency in the capture of eggs, evidenced by the high values of ovitraps positive index and eggs density index, besides that one of the models captured Aedes spp. adults in both regions where they were tested. Therefore, the traps have potential for reducing Aedes spp. eggs and adults in the environment and sensibility for determining the local infestation index, which can be reconciled with official government strategies for more accurate vector monitoring and control actions.

scholarly journals Cas12a-based on-site and rapid nucleic acid detection of African swine fever

2019 ◽  
Author(s):  
Jing Bai ◽  
Haosi Lin ◽  
Haojian Li ◽  
Yang Zhou ◽  
Junshan Liu ◽  
...  
Keyword(s):  
African Swine Fever Virus ◽  
Low Cost ◽  
African Swine Fever ◽  
Lateral Flow ◽  
Ease Of Use ◽  
Nucleic Acid Detection ◽  
Lateral Flow Strip ◽  
Trade Offs ◽  
Laboratory Facilities ◽  
Sensitivity Specificity

AbstractThe mortality rate of hemorrhagic African swine fever (ASF), which targets domestic pigs and is caused by African swine fever virus (ASFV), can reach 100%. ASF has been reported in 25 Chinese provinces since August 2018. There is no effective treatment or vaccine for it and the present molecular diagnosis technologies have trade-offs in sensitivity, specificity, cost and speed, and none of them cater perfectly to ASF control. Thus, a technology that overcomes the need for laboratory facilities, is relatively low cost, and rapidly and sensitively detects ASFV would be highly valuable. Here, we describe an RAA-Cas12a-based system that combines recombinase-aided amplification (RAA) and CRISPR/Cas12a for ASFV detection. The fluorescence intensity readout of this system detected ASFV p72 gene levels as low as 10 aM. For on-site ASFV detection, lateral-flow strip readout was introduced for the first time in the RAA-Cas12a based system (named CORDS, Cas12a-based On-site and Rapid Detection System). We used CORDS to detect target DNA highly specifically using the lateral-flow strip readout. CORDS could identify the p72 gene at femtomolar sensitivity in an hour at 37°C, and only requires an incubator. For ease of use, the regents of CORDS was lyophilized to three tubes and remained the same sensitivity when stored at 4 °C for at least 7 days. Thus, CORDS provides a rapid, sensitive and easily operable method for ASFV on-site detection. Lyophilized CORDS can withstand long-term transportation and storage, and is ready for field applications.

scholarly journals The Advent of Salivary Breast Cancer Biomarker Detection Using Affinity Sensors

Sensors ◽  
2019 ◽  
Vol 19 (10) ◽  
pp. 2373 ◽  
Author(s):  
Imad Abrao Nemeir ◽  
Joseph Saab ◽  
Walid Hleihel ◽  
Abdelhamid Errachid ◽  
Nicole Jafferzic-Renault ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Low Cost ◽  
Current Trend ◽  
High Sensitivity ◽  
Breast Cancer Diagnosis ◽  
Current Status ◽  
Attractive Alternative ◽  
Early Screening ◽  
Screening Programs ◽  
Sensitivity And Selectivity

Breast Cancer is one of the world’s most notorious diseases affecting two million women in 2018 worldwide. It is a highly heterogeneous disease, making it difficult to treat. However, its linear progression makes it a candidate for early screening programs, and the earlier its detection the higher the chance of recovery. However, one key hurdle for breast cancer screening is the fact that most screening techniques are expensive, time-consuming, and cumbersome, making them impractical for use in several parts of the world. One current trend in breast cancer detection has pointed to a possible solution, the use of salivary breast cancer biomarkers. Saliva is an attractive medium for diagnosis because it is readily available in large quantities, easy to obtain at low cost, and contains all the biomarkers present in blood, albeit in lower quantities. Affinity sensors are devices that detect molecules through their interactions with biological recognition molecules. Their low cost, high sensitivity, and selectivity, as well as rapid detection time make them an attractive alternative to traditional means of detection. In this review article, we discuss the current status of breast cancer diagnosis, its salivary biomarkers, as well as the current trends in the development of affinity sensors for their detection.

scholarly journals Nanozyme Applications: A Glimpse of Insight in Food Safety

2021 ◽  
Vol 9 ◽  
Author(s):  
Long Wu ◽  
Shuhong Zhou ◽  
Gonglei Wang ◽  
Yonghuan Yun ◽  
Guozhen Liu ◽  
...  
Keyword(s):  
Low Cost ◽  
Analytical Techniques ◽  
High Sensitivity ◽  
Food Contaminants ◽  
Electrochemical Assay ◽  
Enzyme Mimicking ◽  
Surface Enhanced Raman ◽  
Food Applications ◽  
Enhanced Raman Scattering ◽  
Sensitivity Specificity

Nanozymes own striking merits, including high enzyme-mimicking activity, good stability, and low cost. Due to the powerful and distinguished functions, nanozymes exhibit widespread applications in the field of biosensing and immunoassay, attracting researchers in various fields to design and engineer nanozymes. Recently, nanozymes have been innovatively used to bridge nanotechnology with analytical techniques to achieve the high sensitivity, specificity, and reproducibility. However, the applications of nanozymes in food applications are seldom reviewed. In this review, we summarize several typical nanozymes and provide a comprehensive description of the history, principles, designs, and applications of nanozyme-based analytical techniques in food contaminants detection. Based on engineering and modification of nanozymes, the food contaminants are classified and then discussed in detail via discriminating the roles of nanozymes in various analytical methods, including fluorescence, colorimetric and electrochemical assay, surface-enhanced Raman scattering, magnetic relaxing sensing, and electrochemiluminescence. Further, representative examples of nanozymes-based methods are highlighted for contaminants analysis and inhibition. Finally, the current challenges and prospects of nanozymes are discussed.

On–Chip Droplets Sensing using Capacitive Technique

2013 ◽  
Vol 61 (2) ◽  
Author(s):  
Mohamad Faizal Abdullah ◽  
P. L. Leow ◽  
M. A. Abd Razak ◽  
F. K. Che Harun
Keyword(s):  
Low Cost ◽  
Microfluidic Channel ◽  
High Sensitivity ◽  
Microfluidic Devices ◽  
Capacitive Sensor ◽  
Sample Volume ◽  
Microfluidic System ◽  
Coplanar Electrodes ◽  
On Chip ◽  
Significant Attention

Significant attention has been given on the development of droplets–based microfluidic system because of its potential and apparent advantages. Beside the advantages of reducing the sample volume, it’s also offer less time consuming for the analysis. Optical and fluorescence among the famous method that was used in detection of droplets but they are normally bulky, expensive and not easily accessed. This paper proposed a simple, low cost and high sensitivity for droplets sensing in microfluidic devices by using capacitive sensor. Coplanar electrodes are used to form a capacitance through the microfluidic channel. The design of eight pair of electrodes was used to detect the presence of a droplet. Changes in capacitance due to the presence of a droplet in the sensing area is detected and used to trigger the microscope to capture the image of detected droplets in microchannel. The measurement of droplets detected and counting are displayed through a LABVIEW interface in the real time.

scholarly journals Regenerable Bead-Based Microfluidic Device with integrated THIN-Film Photodiodes for Real Time Monitoring of DNA Detection

Proceedings ◽  
2018 ◽  
Vol 2 (13) ◽  
pp. 953
Author(s):  
Catarina R. F. Caneira ◽  
Denis R. Santos ◽  
Virginia Chu ◽  
João P. Conde
Keyword(s):  
Thin Film ◽  
Real Time ◽  
Microfluidic Device ◽  
Low Cost ◽  
High Sensitivity ◽  
Significant Loss ◽  
Real Time Monitoring ◽  
Target Dna ◽  
On Chip ◽  
Biosensing System

Nanoporous microbead-based microfluidic systems for biosensing applications allow enhanced sensitivities, while being low cost and amenable for miniaturization. The regeneration of the microfluidic biosensing system results in a further decrease in costs while the integration of on-chip signal transduction enhances portability. Here, we present a regenerable bead-based microfluidic device, with integrated thin-film photodiodes, for real-time monitoring of molecular recognition between a target DNA and complementary DNA (cDNA). High-sensitivity assay cycles could be performed without significant loss of probe DNA density and activity, demonstrating the potential for reusability, portability and reproducibility of the system.

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