scholarly journals Competitive Real-Time Near Infrared (NIR) Vein Finder Imaging Device to Improve Peripheral Subcutaneous Vein Selection in Venipuncture for Clinical Laboratory Testing

Micromachines ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 373
Author(s):  
Mark D. Francisco ◽  
Wen-Fan Chen ◽  
Cheng-Tang Pan ◽  
Ming-Cheng Lin ◽  
Zhi-Hong Wen ◽  
...  
Keyword(s):  
Real Time ◽  
Near Infrared ◽  
Imaging System ◽  
Low Cost ◽  
Oxide Semiconductor ◽  
Assessment Process ◽  
Source Image ◽  
Imaging Device ◽  
Dorsal Hand ◽  
Vein Imaging

In this study, near-infrared (NIR) technology was utilized to develop a low-cost real-time near infrared (NIR) guiding device for cannulation. A portable device that can be used by medical practitioners and also by students for their skills development training in performing cannulation. Methods. First, is the development of a reflectance type optical vein finder using three (3) light emitting diode (LED) lights with 960 nm wavelength, complementary metal-oxide-semiconductor-infrared (CMOS-IR) sensor camera with 1920 × 1080 UXGA (1080P), IR filter set for the given wavelength, and an open-source image processing software. Second, is the actual in-vitro human testing in two sites: the arm and dorsal hand of 242 subjects. The following parameters were included, such as gender, age, mass index (BMI), and skin tone. In order to maximize the assessment process towards the device, the researchers included the arm circumference. This augmented subcutaneous vein imaging study using the develop vein finder device compared the difference in the captured vein images through visual and digital imaging approaches. The human testing was performed in accordance with the ethical standards of the Trinity University of Asia—Institutional Ethics Review Committee (TUA—IERC). Results. The NIR imaging system of the developed vein finder in this study showed its capability as an efficient guiding device through real-time vein pattern recognition, for both sites. Improved captured vein images were observed, having 100% visibility of vein patterns on the dorsal hand site. Fourteen (5.79%) out of 242 subjects reported non-visible peripheral subcutaneous veins in the arm sites. Conclusions. The developed vein finder device with the NIR technology and reflected light principle with low-energy consumption was efficient for real-time peripheral subcutaneous vein imaging without the application of a tourniquet. This might be utilized as a guiding device in locating the vein for the purpose of cannulation, at a very low cost as compared to the commercially available vein finders. Moreover, it may be used as an instructional device for student training in performing cannulation.

The near infrared imaging system for the real-time protection of the JET ITER-like wall

2017 ◽  
Vol T170 ◽  
pp. 014027 ◽  
Author(s):  
A Huber ◽  
D Kinna ◽  
V Huber ◽  
G Arnoux ◽  
I Balboa ◽  
...  
Keyword(s):  
Real Time ◽  
Near Infrared ◽  
Infrared Imaging ◽  
Imaging System ◽  
Near Infrared Imaging ◽  
The Real ◽  
Infrared Imaging System

Near-infrared fundus imaging system with light illumination from an electronic contact lens

2022 ◽  
Vol 15 (2) ◽  
pp. 027001
Author(s):  
Yang Cui ◽  
Taiki Takamatsu ◽  
Koichi Shimizu ◽  
Takeo Miyake
Keyword(s):  
Contact Lens ◽  
Near Infrared ◽  
Imaging System ◽  
Low Cost ◽  
Light Illumination ◽  
Fundus Images ◽  
Fundus Imaging ◽  
Point Spread ◽  
Spread Function ◽  
Dependent Point

Abstract As for the diagnosis and treatment of eye diseases, an ideal fundus imaging system is expected to be portability, low cost, and high resolution. Here, we demonstrate a non-mydriatic near-infrared fundus imaging system with light illumination from an electronic contact lens (E-lens). The E-lens can illuminate the retinal and choroidal structures for capturing the fundus images when voltage is applied wirelessly to the lens. And we also reconstruct the images with a depth-dependent point-spread function to suppress the scattering effect that eventually visualizes the clear fundus images.

Real-time dual-modal vein imaging system

2018 ◽  
Vol 14 (2) ◽  
pp. 203-213 ◽  
Author(s):  
Christopher A. Mela ◽  
David P. Lemmer ◽  
Forrest Sheng Bao ◽  
Francis Papay ◽  
Tyler Hicks ◽  
...  
Keyword(s):  
Real Time ◽  
Imaging System ◽  
Vein Imaging

scholarly journals Up-Conversion Sensing of 2D Spatially-Modulated Infrared Information-Carrying Beams with Si-Based Cameras

Sensors ◽  
2020 ◽  
Vol 20 (12) ◽  
pp. 3610
Author(s):  
Adrián J. Torregrosa ◽  
Emir Karamehmedović ◽  
Haroldo Maestre ◽  
María Luisa Rico ◽  
Juan Capmany
Keyword(s):  
Optical Communications ◽  
Near Infrared ◽  
Nonlinear Crystal ◽  
Signal To Noise Ratio ◽  
Low Cost ◽  
Infrared Image ◽  
Complementary Metal Oxide Semiconductor ◽  
Local Oscillator ◽  
Oxide Semiconductor ◽  
Free Space Optical

Up-conversion sensing based on optical heterodyning of an IR (infrared) image with a local oscillator laser wave in a nonlinear optical sum-frequency mixing (SFM) process is a practical solution to circumvent some limitations of IR image sensors in terms of signal-to-noise ratio, speed, resolution, or cooling needs in some demanding applications. In this way, the spectral content of an IR image can become spectrally shifted to the visible/near infrared (VIS/NWIR) and then detected with silicon focal plane arrayed sensors (Si-FPA), such as CCD/CMOS (charge-coupled and complementary metal-oxide-semiconductor devices). This work is an extension of a previous study where we recently introduced this technique in the context of optical communications, in particular in FSOC (free-space optical communications). Herein, we present an image up-conversion system based on a 1064 nm Nd3+: YVO4 solid-state laser with a KTP (potassium titanyl phosphate) nonlinear crystal located intra-cavity where a laser beam at 1550 nm 2D spatially-modulated with a binary Quick Response (QR) code is mixed, giving an up-converted code image at 631 nm that is detected with an Si-based camera. The underlying technology allows for the extension of other IR spectral allocations, construction of compact receivers at low cost, and provides a natural way for increased protection against eavesdropping.

scholarly journals A Quantitative Method to Measure Low Levels of ROS in Nonphagocytic Cells by Using a Chemiluminescent Imaging System

2019 ◽  
Vol 2019 ◽  
pp. 1-11 ◽  
Author(s):  
Jun-Sub Kim ◽  
Kyuho Jeong ◽  
James M. Murphy ◽  
Yelitza A. R. Rodriguez ◽  
Ssang-Taek Steve Lim
Keyword(s):  
Real Time ◽  
Intracellular Signaling ◽  
Imaging System ◽  
Low Cost ◽  
Fiber Formation ◽  
Imaging Method ◽  
Actin Stress Fiber ◽  
Dependent Manner ◽  
Protein Tyrosine ◽  
Low Levels

Chemiluminescence (CL) is one of the most useful methods for detecting reactive oxygen species (ROS). Although fluorescence dyes or genetically encoded biosensors have been developed, CL is still used due to its high sensitivity, ease of use, and low cost. While initially established and used to measure high levels of ROS in phagocytic cells, CL assays are not ideal for measuring low levels of ROS. Here, we developed a newly modified CL assay using a chemiluminescent imaging system for measuring low concentrations of ROS in nonphagocytic cells. We found that dissolving luminol in NaOH, rather than DMSO, increased the H2O2-induced CL signal and that the addition of 4-iodophenylboronic acid (4IPBA) further increased CL intensity. Our new system also increased the rate and intensity of the CL signal in phorbol 12-myristate 13-acetate- (PMA-) treated HT-29 colon cancer cells compared to those in luminol only. We were able to quantify ROS levels from both cells and media in parallel using an H2O2standard. A significant benefit to our system is that we can easily measure stimulus-induced ROS formation in a real-time manner and also investigate intracellular signaling pathways from a single sample simultaneously. We found that PMA induced tyrosine phosphorylation of protein tyrosine kinases (PTKs), such as focal adhesion kinase (FAK), protein tyrosine kinase 2 (Pyk2), and Src, and increased actin stress fiber formation in a ROS-dependent manner. Interestingly, treatment with either N-acetyl-L-cysteine (NAC) or diphenyleneiodonium (DPI) reduced the PMA-stimulated phosphorylation of these PTKs, implicating a potential role in cellular ROS signaling. Thus, our newly optimized CL assay using 4IPBA and a chemiluminescent imaging method provides a simple, real-time, and low-cost method for the quantification of low levels of ROS.

scholarly journals Probeless non-invasive near-infrared spectroscopic bioprocess monitoring using microspectrometer technology

2019 ◽  
Vol 412 (9) ◽  
pp. 2103-2109 ◽  
Author(s):  
Robert Zimmerleiter ◽  
Julian Kager ◽  
Ramin Nikzad-Langerodi ◽  
Vladimir Berezhinskiy ◽  
Frank Westad ◽  
...  
Keyword(s):  
Real Time ◽  
Process Parameters ◽  
Near Infrared ◽  
Low Cost ◽  
Phenoxyacetic Acid ◽  
Bioprocess Monitoring ◽  
Fed Batch ◽  
Precise Control ◽  
Fed Batch Fermentation ◽  
Using Data

AbstractReal-time measurements and adjustments of critical process parameters are essential for the precise control of fermentation processes and thus for increasing both quality and yield of the desired product. However, the measurement of some crucial process parameters such as biomass, product, and product precursor concentrations usually requires time-consuming offline laboratory analysis. In this work, we demonstrate the in-line monitoring of biomass, penicillin (PEN), and phenoxyacetic acid (POX) in a Penicilliumchrysogenum fed-batch fermentation process using low-cost microspectrometer technology operating in the near-infrared (NIR). In particular, NIR reflection spectra were taken directly through the glass wall of the bioreactor, which eliminates the need for an expensive NIR immersion probe. Furthermore, the risk of contaminations in the reactor is significantly reduced, as no direct contact with the investigated medium is required. NIR spectra were acquired using two sensor modules covering the spectral ranges 1350–1650 nm and 1550–1950 nm. Based on offline reference analytics, partial least squares (PLS) regression models were established for biomass, PEN, and POX either using data from both sensors separately or jointly. The established PLS models were tested on an independent validation fed-batch experiment. Root mean squared errors of prediction (RMSEP) were 1.61 g/L, 1.66 g/L, and 0.67 g/L for biomass, PEN, and POX, respectively, which can be considered an acceptable accuracy comparable with previously published results using standard process spectrometers with immersion probes. Altogether, the presented results underpin the potential of low-cost microspectrometer technology in real-time bioprocess monitoring applications.

Image-guided near-infrared fluorescent sentinel lymph node mapping in human breast cancer

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. e11591-e11591
Author(s):  
S. Troyan ◽  
S. Gibbs-Strauss ◽  
S. Gioux ◽  
R. Oketokoun ◽  
F. Azar ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Optical Imaging ◽  
Real Time ◽  
Near Infrared ◽  
Imaging System ◽  
Sulfur Colloid ◽  
Guided Surgery ◽  
Image Guided ◽  
Nir Fluorescence ◽  
Surgical Field

e11591 Background: Breast cancer surgery is presently performed without real-time image-guidance. We have developed a novel optical imaging system for image-guided surgery that uses invisible near-infrared (NIR) fluorescent light to highlight structures on the surgical field with high sensitivity, specificity, and contrast. We have also performed the first human clinical trial of the imaging system in women undergoing SLN mapping for breast cancer. Methods: We used a portable imaging system with an articulating arm that has 6 degrees of freedom, high power LED light source, custom optics, custom software, and sterile drape. The imaging system provided simultaneous and real-time imaging of color video and NIR fluorescence at up to 15 frames per second. N = 6 women with biopsy- confirmed breast cancer undergoing SLN mapping gave informed consent. All subjects received conventional mapping with Tc-99m sulfur colloid using a handheld gamma probe as well as NIR fluorescence-guided SLN mapping using a mixture of indocyanine green (ICG) diluted to a final concentration of 10 μM in human serum albumin (ICG:HSA). Results: The imaging system was easy to position in the operating room, with the articulating arm providing 50” horizontal reach and 70” vertical reach. Working distance to the patient was 18”. NIR fluorescence excitation was 20 mW/cm2 at 760 nm. NIR-depleted white light was 40,000 lux. A total of 1.6 ml of ICG:HSA was injected intra-tumorally and peri-tumorally and the site massaged for 5 min. 8 of 9 SLNs identified by Tc- 99m sulfur colloid were also identified by NIR fluorescence. However, NIR fluorescence identified an SLN, confirmed to have cancer in it, that was not identified by Tc-99m sulfur colloid. These differences were consistent with asynchrony in the injection techniques. Unlike the gamma-ray probe, NIR fluorescence provided high-resolution, large area optical imaging of the surgical field, and helped guide surgical resection. Conclusions: In this 6-patient pilot study, a novel NIR fluorescence optical imaging system was used for the first time, and provided real-time image-guided surgery for SLN mapping of breast cancer. No significant financial relationships to disclose.

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