scholarly journals Integration of Hierarchical Micro-/Nanostructures in a Microfluidic Chip for Efficient and Selective Isolation of Rare Tumor Cells

Micromachines ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 698
Author(s):  
Shunqiang Wang ◽  
Younghyun Cho ◽  
Xuanhong Cheng ◽  
Shu Yang ◽  
Yi Liu ◽  
...  
Keyword(s):  
Shear Rate ◽  
Tumor Cells ◽  
Microfluidic Chip ◽  
Volume Ratio ◽  
Capture Efficiency ◽  
Technological Advancement ◽  
Clinical Markers ◽  
Diagnosis And Prognosis ◽  
Herringbone Structure ◽  
Isolation Performance

Circulating tumor cells (CTCs) are important clinical markers for both cancer early diagnosis and prognosis. Various techniques have been developed in the past decade to isolate and quantify these cells from the blood while microfluidic technology attracts significant attention due to better controlled microenvironment. When combined with advanced nanotechnologies, CTC isolation performance in microfluidic devices can be further improved. In this article, by extending the wavy-herringbone concept developed earlier in our team, we prepared a hierarchical microfluidic chip by introducing a uniform coating of nanoparticles with anti-epithelial cell adhesion molecule (EpCAM) on wavy microgrooves. This hierarchical structured platform not only maintains the capture purity of the wavy-herringbone structure but improves the capture efficiency thanks to the larger surface area to volume ratio brought by nanoparticles. Our results demonstrated a capture efficiency of almost 100% at a low shear rate of 60/s. Even at a higher shear rate of 400/s, the hierarchical micro/nanostructures demonstrated an enhancement of up to ~3-fold for capture efficiency (i.e., 70%) and ~1.5-fold for capture purity (i.e., 68%), compared to wavy-herringbone structures without nanoparticle coating. With these promising results, this hierarchical structured platform represents a technological advancement for CTC isolation and cancer care.

A microfluidic chip with double-sided herringbone microstructures for enhanced capture of rare tumor cells

2017 ◽  
Vol 5 (46) ◽  
pp. 9114-9120 ◽  
Author(s):  
Minjiao Wang ◽  
Zhihua Wang ◽  
Mingkan Zhang ◽  
Wei Guo ◽  
Ning Li ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Analytical Model ◽  
Microfluidic Chip ◽  
Capture Efficiency ◽  
Rare Tumor

This paper reports an analytical model to geometrically optimize a herringbone chip by investigating the interactions between cells and antibody-immobilized device surfaces for enhancing CTC capture efficiency.

Site-specific antibody modification and immobilization on a microfluidic chip to promote the capture of circulating tumor cells and microemboli

2017 ◽  
Vol 53 (29) ◽  
pp. 4152-4155 ◽  
Author(s):  
Chian-Hui Lai ◽  
Syer Choon Lim ◽  
Liang-Chun Wu ◽  
Chien-Fang Wang ◽  
Wen-Sy Tsai ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Specific Antibody ◽  
Microfluidic Chip ◽  
Capture Efficiency ◽  
Site Specific ◽  
Antibody Coating

Antibody coating via Fc-domain site-specific immobilization improves capture efficiency of circulating tumor cells and microemboli in microfluidics.

scholarly journals Circulating Tumor Cells from Enumeration to Analysis: Current Challenges and Future Opportunities

Cancers ◽  
2021 ◽  
Vol 13 (11) ◽  
pp. 2723
Author(s):  
Yu-Ping Yang ◽  
Teresa M. Giret ◽  
Richard J. Cote
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Ex Vivo ◽  
Detection Sensitivity ◽  
Clinical Utilization ◽  
Diagnosis And Prognosis ◽  
Early Cancer Diagnosis ◽  
Potential Marker ◽  
Potential Applications ◽  
Downstream Analysis

Circulating tumor cells (CTCs) have been recognized as a major contributor to distant metastasis. Their unique role as metastatic seeds renders them a potential marker in the circulation for early cancer diagnosis and prognosis as well as monitoring of therapeutic response. In the past decade, researchers mainly focused on the development of isolation techniques for improving the recovery rate and purity of CTCs. These developed techniques have significantly increased the detection sensitivity and enumeration accuracy of CTCs. Currently, significant efforts have been made toward comprehensive molecular characterization, ex vivo expansion of CTCs, and understanding the interactions between CTCs and their associated cells (e.g., immune cells and stromal cells) in the circulation. In this review, we briefly summarize existing CTC isolation technologies and specifically focus on advances in downstream analysis of CTCs and their potential applications in precision medicine. We also discuss the current challenges and future opportunities in their clinical utilization.

scholarly journals Microfluidic Chip-Based Cancer Diagnosis and Prediction of Relapse by Detecting Circulating Tumor Cells and Circulating Cancer Stem Cells

Cancers ◽  
2021 ◽  
Vol 13 (6) ◽  
pp. 1385
Author(s):  
Hyeon-Yeol Cho ◽  
Jin-Ha Choi ◽  
Joungpyo Lim ◽  
Sang-Nam Lee ◽  
Jeong-Woo Choi
Keyword(s):  
Stem Cells ◽  
Cancer Stem Cells ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Metastatic Cancer ◽  
Cancer Prognosis ◽  
Optical Biosensors ◽  
Diagnosis And Prognosis ◽  
Noise Factors ◽  
Prediction Of Relapse

Detecting circulating tumor cells (CTCs) has been considered one of the best biomarkers in liquid biopsy for early diagnosis and prognosis monitoring in cancer. A major challenge of using CTCs is detecting extremely low-concentrated targets in the presence of high noise factors such as serum and hematopoietic cells. This review provides a selective overview of the recent progress in the design of microfluidic devices with optical sensing tools and their application in the detection and analysis of CTCs and their small malignant subset, circulating cancer stem cells (CCSCs). Moreover, discussion of novel strategies to analyze the differentiation of circulating cancer stem cells will contribute to an understanding of metastatic cancer, which can help clinicians to make a better assessment. We believe that the topic discussed in this review can provide brief guideline for the development of microfluidic-based optical biosensors in cancer prognosis monitoring and clinical applications.

scholarly journals Patients Characteristics in a General Intensive Care Unit (GICU): Facts and Outcomes

KYAMC Journal ◽  
2017 ◽  
Vol 7 (1) ◽  
pp. 678-680
Author(s):  
Kamrunnahar Alo ◽  
Md Mahfuzar Rahman ◽  
Rahena Khatun
Keyword(s):  
Tertiary Care ◽  
Support Service ◽  
Admission Diagnosis ◽  
Technological Advancement ◽  
Management Skills ◽  
College Hospital ◽  
Team Members ◽  
Medical College ◽  
Diagnosis And Prognosis ◽  
Management Protocol

Background: Patient's management during emergency differs significantly between countries. However, technological advancement and requirement of quality support service as well as survivality is a great concern towards reduced hospital mortality.Aim and Objectives: The aim of this study is to find out patient's characteristics admitted at GICU of Khwaja Yunus Ali Medical College Hospital (KYAMCH) in order to investigate selected variables like; the duration of stay at GICU, their health problems on admission, diagnosis and prognosis including certain demographic characteristics in particular.Methods and Materials: This was an exploratory study on patient's characteristics during the period from January to June 2015 at GICU of KYAMCH. The data were collected through record review according to variables available on GICU admission register.Results: Total 163 cases were studied in 6 months. Among them 98 (60.12%. were male & 65 (39.88%) were female. The mean age of admitted male patients were 47 & in female it was 40 years with mean ± 16 and 17 years respectively. In addition GICU stays were found up to 5 days among 76.58% patients. The study revealed septic shock, cancer, injury and CVD as the common emergencies that needed skillful attention. Moreover, death and Discharge on Risk Bond (DORB) were found unwanted outcomes (52.23%) that also need to be addressed through developing skilled management protocol at GICU of concern tertiary care hospital.Conclusions: The findings will help hospital managers to identify necessary support needed as well. It will also help to identify emergency management skills needed for the GICU personnel team members in particular. Thus it can be a basis for the hospital administrators to take appropriate measures in towards reducing hospital mortality rate as well.KYAMC Journal Vol. 7, No.-1, Jul 2016, Page 678-680

scholarly journals A Novel Microfluidic Chip for Fast, Sensitive Quantification of Plasma Extracellular Vesicles as Biomarkers in Patients With Osteosarcoma

2021 ◽  
Vol 11 ◽  
Author(s):  
Yi-Qi Xu ◽  
Qi-Yuan Bao ◽  
Sai-Xi Yu ◽  
Qi Liu ◽  
Yan Xie ◽  
...  
Keyword(s):  
Microfluidic Chip ◽  
Limit Of Detection ◽  
Zno Nanorods ◽  
Extracellular Vesicle ◽  
Fluorescent Labeling ◽  
Total Plasma ◽  
Sarcoma Cell ◽  
Diagnosis And Prognosis ◽  
Healthy Donors ◽  
Capture Antibody

Plasma circulating extracellular vesicle (EV) has emerged as a promising biomarker for diagnosis and prognosis of various epithelial tumors. However, fast and efficient capture of EVs with microfluidic chip in sarcoma remains to be established. Herein, we reported a ZnO-nanorods integrated (ZNI) microfluidic chip, where EV capture antibody was uniformly grafted to the surface of the ZnO-nanorods of the chip to enhance the plasma turbulence formation and the capture efficiency at the micro-scale. Based on osteosarcoma (OS) cell line, we demonstrated that a combination of CD81 and CD63 antibody on ZNI chip yielded the greatest amount of total EVs, with an extra sensitive limit of detection (LOD) of ~104 particles mL-1. Furthermore, the addition of fluorescent labeling of Vimentin (VIM), a previously reported sarcoma cell surface biomarker, could enabled the dual visualization of total plasma EVs and VIM-positive EVs from OS patients’ plasma. Based on our ZNI chip, we found that the amount of plasma total EVs was significantly different between OS and healthy donors (1562 a.u. versus 639 a.u., p< 0.05), but not between metastatic and nonmetastatic OS (p> 0.05). Interestingly, patients with metastatic disease had a significantly greater amount of VIM-positive EVs (1411 a.u. versus 231 a.u.., p< 0.05) and increased VIM-positive/total EVs ratio (0.943 versus 0.211, p< 0.05) in comparison with the nonmetastatic counterpart. Therefore, our ZNI microfluidic chip has great potential for the fast quantification of plasma EVs, and the microfluidic-based quantification of total and VIM-positive EVs might serve as a promising biomarker for the diagnosis and surveillance in OS patients.

Integration of aligned polymer nanofibers within a microfluidic chip for efficient capture and rapid release of circulating tumor cells

2018 ◽  
Vol 2 (5) ◽  
pp. 891-900 ◽  
Author(s):  
Yunchao Xiao ◽  
Mengyuan Wang ◽  
Lizhou Lin ◽  
Lianfang Du ◽  
Mingwu Shen ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Microfluidic Chip ◽  
Polymer Nanofibers ◽  
Highly Efficient ◽  
Rapid Release ◽  
Aligned Nanofibers

Zwitterion-functionalized aligned nanofibers integrated with a microfluidic chip can be used for highly efficient capture and rapid release of CTCs.

High-performance multiplex microvalves fabrication and using for tumor cells staining on a microfluidic chip

2019 ◽  
Vol 21 (4) ◽  
Author(s):  
Shao-Li Hong ◽  
Man Tang ◽  
Zhengqi Chen ◽  
Zhao Ai ◽  
Feng Liu ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Microfluidic Chip ◽  
High Performance
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