A microfluidic chip with double-sided herringbone microstructures for enhanced capture of rare tumor cells

2017 ◽  
Vol 5 (46) ◽  
pp. 9114-9120 ◽  
Author(s):  
Minjiao Wang ◽  
Zhihua Wang ◽  
Mingkan Zhang ◽  
Wei Guo ◽  
Ning Li ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Analytical Model ◽  
Microfluidic Chip ◽  
Capture Efficiency ◽  
Rare Tumor

This paper reports an analytical model to geometrically optimize a herringbone chip by investigating the interactions between cells and antibody-immobilized device surfaces for enhancing CTC capture efficiency.

scholarly journals Highly efficient and selective isolation of rare tumor cells using a microfluidic chip with wavy-herringbone micro-patterned surfaces

The Analyst ◽  
2016 ◽  
Vol 141 (7) ◽  
pp. 2228-2237 ◽  
Author(s):  
Shunqiang Wang ◽  
Antony Thomas ◽  
Elaine Lee ◽  
Shu Yang ◽  
Xuanhong Cheng ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Microfluidic Chip ◽  
Patterned Surfaces ◽  
Rare Tumor ◽  
Selective Isolation ◽  
Highly Efficient

We present a microfluidic chip with wavy-herringbone micro-patterned surfaces for highly efficient and selective isolation of viable rare tumor cells.

Site-specific antibody modification and immobilization on a microfluidic chip to promote the capture of circulating tumor cells and microemboli

2017 ◽  
Vol 53 (29) ◽  
pp. 4152-4155 ◽  
Author(s):  
Chian-Hui Lai ◽  
Syer Choon Lim ◽  
Liang-Chun Wu ◽  
Chien-Fang Wang ◽  
Wen-Sy Tsai ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Specific Antibody ◽  
Microfluidic Chip ◽  
Capture Efficiency ◽  
Site Specific ◽  
Antibody Coating

Antibody coating via Fc-domain site-specific immobilization improves capture efficiency of circulating tumor cells and microemboli in microfluidics.

scholarly journals Integration of Hierarchical Micro-/Nanostructures in a Microfluidic Chip for Efficient and Selective Isolation of Rare Tumor Cells

Micromachines ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 698
Author(s):  
Shunqiang Wang ◽  
Younghyun Cho ◽  
Xuanhong Cheng ◽  
Shu Yang ◽  
Yi Liu ◽  
...  
Keyword(s):  
Shear Rate ◽  
Tumor Cells ◽  
Microfluidic Chip ◽  
Volume Ratio ◽  
Capture Efficiency ◽  
Technological Advancement ◽  
Clinical Markers ◽  
Diagnosis And Prognosis ◽  
Herringbone Structure ◽  
Isolation Performance

Circulating tumor cells (CTCs) are important clinical markers for both cancer early diagnosis and prognosis. Various techniques have been developed in the past decade to isolate and quantify these cells from the blood while microfluidic technology attracts significant attention due to better controlled microenvironment. When combined with advanced nanotechnologies, CTC isolation performance in microfluidic devices can be further improved. In this article, by extending the wavy-herringbone concept developed earlier in our team, we prepared a hierarchical microfluidic chip by introducing a uniform coating of nanoparticles with anti-epithelial cell adhesion molecule (EpCAM) on wavy microgrooves. This hierarchical structured platform not only maintains the capture purity of the wavy-herringbone structure but improves the capture efficiency thanks to the larger surface area to volume ratio brought by nanoparticles. Our results demonstrated a capture efficiency of almost 100% at a low shear rate of 60/s. Even at a higher shear rate of 400/s, the hierarchical micro/nanostructures demonstrated an enhancement of up to ~3-fold for capture efficiency (i.e., 70%) and ~1.5-fold for capture purity (i.e., 68%), compared to wavy-herringbone structures without nanoparticle coating. With these promising results, this hierarchical structured platform represents a technological advancement for CTC isolation and cancer care.

Integration of aligned polymer nanofibers within a microfluidic chip for efficient capture and rapid release of circulating tumor cells

2018 ◽  
Vol 2 (5) ◽  
pp. 891-900 ◽  
Author(s):  
Yunchao Xiao ◽  
Mengyuan Wang ◽  
Lizhou Lin ◽  
Lianfang Du ◽  
Mingwu Shen ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Microfluidic Chip ◽  
Polymer Nanofibers ◽  
Highly Efficient ◽  
Rapid Release ◽  
Aligned Nanofibers

Zwitterion-functionalized aligned nanofibers integrated with a microfluidic chip can be used for highly efficient capture and rapid release of CTCs.

High-performance multiplex microvalves fabrication and using for tumor cells staining on a microfluidic chip

2019 ◽  
Vol 21 (4) ◽  
Author(s):  
Shao-Li Hong ◽  
Man Tang ◽  
Zhengqi Chen ◽  
Zhao Ai ◽  
Feng Liu ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Microfluidic Chip ◽  
High Performance

Integrated Microfluidic Chip for Efficient Isolation and Deformability Analysis of Circulating Tumor Cells

2018 ◽  
Vol 2 (10) ◽  
pp. 1800200 ◽  
Author(s):  
Zongbin Liu ◽  
Rui Chen ◽  
Ying Li ◽  
Jianqiao Liu ◽  
Ping Wang ◽  
...  
Keyword(s):  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Microfluidic Chip

scholarly journals Primary epithelioid rhabdomyosarcoma of the stomach: a case report and review of literature

2019 ◽  
Vol 14 (1) ◽  
Author(s):  
Yangkun Wang ◽  
Pei Guo ◽  
Zhishang Zhang ◽  
Runde Jiang ◽  
Zuguo Li
Keyword(s):  
Tumor Cells ◽  
Unusual Case ◽  
Histopathological Examination ◽  
Gastroesophageal Junction ◽  
Rare Tumor ◽  
Review Of Literature ◽  
Ki 67 ◽  
Case Presentation ◽  
Cytoplasmic Content ◽  
Mitotic Figures

Abstract Background Epithelioid rhabdomyosarcoma is a rare tumor that generally occurs in the bladder, the parotid gland, or the skin of the neck. We describe an unusual case of primary epithelioid rhabdomyosarcoma of the stomach and review the literature. Case presentation A 64-year-old woman presented with a lesion at the gastroesophageal junction. Histopathological examination showed irregularly sized round cells with low cytoplasmic content and eccentric nuclei. Mitotic figures were present. Fibrovascular septa and areas of necrosis were observed between tumor cells. Tumor cells were strongly positive for MyoD1, desmin, and myogenin, and weakly positive for actin, CD56, and PGP9.5. The ki-67 index was ≥90%. Conclusions Primary epithelioid rhabdomyosarcoma of the stomach is extremely rare. Better awareness of this entity is necessary for early diagnosis and treatment.

Device for whole genome sequencing single circulating tumor cells from whole blood

Lab on a Chip ◽  
2019 ◽  
Vol 19 (19) ◽  
pp. 3168-3178 ◽  
Author(s):  
Ren Li ◽  
Fei Jia ◽  
Weikai Zhang ◽  
Fanghao Shi ◽  
Zhiguo Fang ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Genome Sequencing ◽  
Microfluidic Chip ◽  
Whole Blood ◽  
Whole Genome

To sequence single circulating tumor cells (CTCs) from whole blood, a microfluidic chip was developed to perform blood filtering/CTC enrichment/CTC sorting and in situ MDA for whole genome sequencing.

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