scholarly journals Real-Time Single Cell Monitoring: Measurement and Counting of Motile Sperm Using LCR Impedance-Integrated Microfluidic Device

Micromachines ◽  
2019 ◽  
Vol 10 (10) ◽  
pp. 647 ◽  
Author(s):  
Chalinee Phiphattanaphiphop ◽  
Komgrit Leksakul ◽  
Rungrueang Phatthanakun ◽  
Apirak Suthummapiwat
Keyword(s):  
Single Cell ◽  
Real Time ◽  
Low Cost ◽  
Semen Analysis ◽  
Sperm Concentration ◽  
Thin Metal Film ◽  
Computer Assisted ◽  
Cell Detection ◽  
Motile Sperm ◽  
Single Cell Detection

In this research, we aimed to count the ratio of the number of motile to immotile sperm for patients with infertility problems based on a low-sperm-concentration examination. The microfluidic system consists of two series of applications: The conventional separation of motile sperm and the proposed inductance (L), capacitance (C), and resistance (R) or LCR impedance sperm counter. In the experiment, 96% of motile sperm were isolated from nonmotile sperm in the first part and transported to the second part to count and calculate real-time sperm concentration. A pair of microelectrodes composed of thin metal film were integrated between microchannels, resulting in a peak signal for LCR single-cell detection, as well as the estimated total sperm concentration. A minimum of 10 µL of the sperm sample was completely analyzed with an accuracy of 94.8% compared with the standard computer-assisted semen analysis (CASA) method. This method could be applied for low-cost sperm separation and counting in the future.

scholarly journals Multiplexed and single-cell detection of microRNA with plasmonic nanoparticle assemblies

2021 ◽  
Author(s):  
Gurbrinder Ghotra ◽  
Nguyen Le ◽  
Heyam Hayder ◽  
Chun Peng ◽  
Jennifer I. L. Chen
Keyword(s):  
Nucleic Acid ◽  
Single Cell ◽  
Low Cost ◽  
Single Cells ◽  
Cell Detection ◽  
Label Free ◽  
Plasmonic Nanoparticle ◽  
Nanoparticle Assemblies ◽  
Single Cell Detection ◽  
Target Binding

We present a label-free, low cost and miniatured biosensing platform based on the disassembly of core-satellite plasmonic nanoparticle assemblies. The rapid and selective detection of an exemplary nucleic acid biomarker, has-miRNA-210-3p, was achieved via the strand displacement nucleic acid reaction. Target binding leads to dehybridization of the DNA linkers and changes in the scattering properties of nanostructures as monitored by darkfield microscopy. We demonstrate the ability to detect microRNA expunged from single cells and the potential to multiplex discrete assemblies to enable diverse biological applicability. The work may help translate the applicability of microRNA as diagnostic biomarkers, quantitate their abundance in the microenvironment, and facilitate the study of their correlation or causation to other biomolecules at the single-cell level.

scholarly journals Fluorescence enhanced BA-LIFT for single cell detection and isolation

2020 ◽  
Vol 12 (2) ◽  
pp. 025019 ◽  
Author(s):  
A Marquez ◽  
M Gómez-Fontela ◽  
S Lauzurica ◽  
R Candorcio-Simón ◽  
D Munoz-Martin ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Detection ◽  
Single Cell Detection

Single-Cell Detection of Trans-Splicing Ribozyme In Vivo Activity

2004 ◽  
Vol 126 (23) ◽  
pp. 7158-7159 ◽  
Author(s):  
Sumitaka Hasegawa ◽  
Jung W. Choi ◽  
Jianghong Rao
Keyword(s):  
Single Cell ◽  
Cell Detection ◽  
Single Cell Detection ◽  
Trans Splicing

The Role of Nanotechnology in Single-Cell Detection: A Review

2014 ◽  
Vol 10 (10) ◽  
pp. 2598-2619 ◽  
Author(s):  
Changling Wang ◽  
Yuxiang Zhang ◽  
Mingdian Xia ◽  
Xingxi Zhu ◽  
Shitao Qi ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Detection ◽  
Single Cell Detection

Single-cell detection by cavity ring-down spectroscopy

2004 ◽  
Vol 85 (19) ◽  
pp. 4523 ◽  
Author(s):  
Peter B. Tarsa ◽  
Aislyn D. Wist ◽  
Paul Rabinowitz ◽  
Kevin K. Lehmann
Keyword(s):  
Single Cell ◽  
Cell Detection ◽  
Cavity Ring Down Spectroscopy ◽  
Single Cell Detection ◽  
Cavity Ring Down

scholarly journals Dielectrophoresis assisted rapid, selective and single cell detection of antibiotic resistant bacteria with G-FETs

2020 ◽  
Vol 156 ◽  
pp. 112123 ◽  
Author(s):  
Narendra Kumar ◽  
Wenjian Wang ◽  
Juan C. Ortiz-Marquez ◽  
Matthew Catalano ◽  
Mason Gray ◽  
...  
Keyword(s):  
Single Cell ◽  
Resistant Bacteria ◽  
Cell Detection ◽  
Antibiotic Resistant Bacteria ◽  
Antibiotic Resistant ◽  
Single Cell Detection

Single‐Cell Detection and Photostimulation on a Microfluidic Chip Aided with Gold Nanorods

2019 ◽  
Vol 97 (1) ◽  
pp. 39-45
Author(s):  
Yujie Zhu ◽  
Hui Xu ◽  
Xunbin Wei ◽  
Hao He
Keyword(s):  
Single Cell ◽  
Gold Nanorods ◽  
Microfluidic Chip ◽  
Cell Detection ◽  
Single Cell Detection

scholarly journals In situ single cell detection via microfluidic magnetic bead assay

PLoS ONE ◽  
2017 ◽  
Vol 12 (2) ◽  
pp. e0172697 ◽  
Author(s):  
Fan Liu ◽  
Pawan KC ◽  
Ge Zhang ◽  
Jiang Zhe
Keyword(s):  
Single Cell ◽  
Magnetic Bead ◽  
Cell Detection ◽  
Single Cell Detection ◽  
Magnetic Bead Assay

scholarly journals In Vivo Single-Cell Detection of Metabolic Oscillations in Stem Cells

Cell Reports ◽  
2015 ◽  
Vol 10 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Chiara Stringari ◽  
Hong Wang ◽  
Mikhail Geyfman ◽  
Viera Crosignani ◽  
Vivek Kumar ◽  
...  
Keyword(s):  
Stem Cells ◽  
Single Cell ◽  
Cell Detection ◽  
Single Cell Detection ◽  
Metabolic Oscillations
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