scholarly journals In Vivo Single-Cell Detection of Metabolic Oscillations in Stem Cells

Cell Reports ◽  
2015 ◽  
Vol 10 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Chiara Stringari ◽  
Hong Wang ◽  
Mikhail Geyfman ◽  
Viera Crosignani ◽  
Vivek Kumar ◽  
...  
Keyword(s):  
Stem Cells ◽  
Single Cell ◽  
Cell Detection ◽  
Single Cell Detection ◽  
Metabolic Oscillations

Single-Cell Detection of Trans-Splicing Ribozyme In Vivo Activity

2004 ◽  
Vol 126 (23) ◽  
pp. 7158-7159 ◽  
Author(s):  
Sumitaka Hasegawa ◽  
Jung W. Choi ◽  
Jianghong Rao
Keyword(s):  
Single Cell ◽  
Cell Detection ◽  
Single Cell Detection ◽  
Trans Splicing

scholarly journals In vivo single cell detection of tumor-infiltrating lymphocytes with a clinical 1.5 Tesla MRI system

2008 ◽  
Vol 60 (6) ◽  
pp. 1292-1297 ◽  
Author(s):  
Pierre Smirnov ◽  
Marie Poirier-Quinot ◽  
Claire Wilhelm ◽  
Elise Lavergne ◽  
Jean-Christophe Ginefri ◽  
...  
Keyword(s):  
Single Cell ◽  
Tumor Infiltrating Lymphocytes ◽  
Cell Detection ◽  
Single Cell Detection ◽  
Infiltrating Lymphocytes

Microfluidics Design for Single Cell Detection

2013 ◽  
Vol 562-565 ◽  
pp. 589-593
Author(s):  
Shao Bo Du ◽  
Sheng Bo Sang ◽  
Wen Dong Zhang ◽  
Jie Hu ◽  
Peng Wei Li ◽  
...  
Keyword(s):  
Single Cell ◽  
Fluid Transport ◽  
Individual Cell ◽  
Single Cells ◽  
Cell Detection ◽  
Cell Capture ◽  
Single Cell Detection ◽  
Novel Structure ◽  
Mother And Daughter

Here we demonstrate a microfluidic-based analysis system based on single cell capture array, which can physically trap individual cell using micrometer-sized structures. A stable and in vivo-like microenvironment was built with the novel structure at the single-cell detection level. The microfluidic-based design can decouple single cells from fluid flow with the help of micropillars. The size and geometry of the cell jails are designed in order to discriminate between mother and daughter cells. It provides an experimental platform to efficiently monitor individual cell state for a long period of time. Furthermore, the parallel microfluidic array can ensure accuracy. In addition, finite element method (FEM) was employed to predict fluid transport properties for the most optimal fluid microenvironment.

scholarly journals Single cell detection of latent cytomegalovirus reactivation in host tissue

2011 ◽  
Vol 92 (6) ◽  
pp. 1279-1291 ◽  
Author(s):  
Anja Marquardt ◽  
Stephan Halle ◽  
Christof K. Seckert ◽  
Niels A. W. Lemmermann ◽  
Tibor Z. Veres ◽  
...  
Keyword(s):  
Single Cell ◽  
Molecular Mechanisms ◽  
Fluorescent Protein ◽  
Single Cells ◽  
Cell Detection ◽  
Cytomegalovirus Reactivation ◽  
Single Cell Detection ◽  
Latently Infected ◽  
Organ Tissue

The molecular mechanisms leading to reactivation of latent cytomegalovirus are not well understood. To study reactivation, the few cells in an organ tissue that give rise to reactivated virus need to be identified, ideally at the earliest possible time point in the process. To this end, mouse cytomegalovirus (MCMV) reporter mutants were designed to simultaneously express the red fluorescent protein mCherry and the secreted Gaussia luciferase (Gluc). Whereas Gluc can serve to assess infection at the level of individual mice by measuring luminescence in blood samples or by in vivo imaging, mCherry fluorescence offers the advatage of detection of infection at the single cell level. To visualize cells in which MCMV was being reactivated, precision-cut lung slices (PCLS) that preserve tissue microanatomy were prepared from the lungs of latently infected mice. By day 3 of cultivation of the PCLS, reactivation was revealed by Gluc expression, preceding the detection of infectious virus by approximately 4 days. Reactivation events in PCLS could be identified when they were still confined to single cells. Notably, using fractalkine receptor–GFP reporter mice, we never observed reactivation originating from CX3CR1+ monocytes or pulmonary dendritic cells derived therefrom. Furthermore, latent viral genome in the lungs was not enriched in sorted bone-marrow-derived cells expressing CD11b. Taken together, these complementary approaches suggest that CD11b+ and CX3CR1+ subsets of the myeloid differentiation lineage are not the main reservoirs and cellular sites of MCMV latency and reactivation in the lungs.

scholarly journals Single-cell RNA sequencing of cultured human endometrial CD140b+CD146+ perivascular cells highlights the importance of in vivo microenvironment

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Dandan Cao ◽  
Rachel W. S. Chan ◽  
Ernest H. Y. Ng ◽  
Kristina Gemzell-Danielsson ◽  
William S. B. Yeung
Keyword(s):  
Stem Cells ◽  
Single Cell ◽  
Rna Sequencing ◽  
Marker Gene ◽  
Cellular Heterogeneity ◽  
Stromal Fibroblast ◽  
Perivascular Cells ◽  
Endometrial Cells ◽  
Single Cell Rna Sequencing

Abstract Background Endometrial mesenchymal-like stromal/stem cells (eMSCs) have been proposed as adult stem cells contributing to endometrial regeneration. One set of perivascular markers (CD140b&CD146) has been widely used to enrich eMSCs. Although eMSCs are easily accessible for regenerative medicine and have long been studied, their cellular heterogeneity, relationship to primary counterpart, remains largely unclear. Methods In this study, we applied 10X genomics single-cell RNA sequencing (scRNA-seq) to cultured human CD140b+CD146+ endometrial perivascular cells (ePCs) from menstrual and secretory endometrium. We also analyzed publicly available scRNA-seq data of primary endometrium and performed transcriptome comparison between cultured ePCs and primary ePCs at single-cell level. Results Transcriptomic expression-based clustering revealed limited heterogeneity within cultured menstrual and secretory ePCs. A main subpopulation and a small stress-induced subpopulation were identified in secretory and menstrual ePCs. Cell identity analysis demonstrated the similar cellular composition in secretory and menstrual ePCs. Marker gene expression analysis showed that the main subpopulations identified from cultured secretory and menstrual ePCs simultaneously expressed genes marking mesenchymal stem cell (MSC), perivascular cell, smooth muscle cell, and stromal fibroblast. GO enrichment analysis revealed that genes upregulated in the main subpopulation enriched in actin filament organization, cellular division, etc., while genes upregulated in the small subpopulation enriched in extracellular matrix disassembly, stress response, etc. By comparing subpopulations of cultured ePCs to the publicly available primary endometrial cells, it was found that the main subpopulation identified from cultured ePCs was culture-unique which was unlike primary ePCs or primary endometrial stromal fibroblast cells. Conclusion In summary, these data for the first time provides a single-cell atlas of the cultured human CD140b+CD146+ ePCs. The identification of culture-unique relatively homogenous cell population of CD140b+CD146+ ePCs underscores the importance of in vivo microenvironment in maintaining cellular identity.

scholarly journals Comparative Analyses of Single-Cell Transcriptomic Profiles between In Vitro Totipotent Blastomere-like Cells and In Vivo Early Mouse Embryonic Cells

Cells ◽  
2021 ◽  
Vol 10 (11) ◽  
pp. 3111
Author(s):  
Po-Yu Lin ◽  
Denny Yang ◽  
Chi-Hsuan Chuang ◽  
Hsuan Lin ◽  
Wei-Ju Chen ◽  
...  
Keyword(s):  
Stem Cells ◽  
Single Cell ◽  
Embryonic Cells ◽  
Cell Stage ◽  
Developmental Potential ◽  
Functional Features ◽  
Early Mouse ◽  
Extraembryonic Tissues

The developmental potential within pluripotent cells in the canonical model is restricted to embryonic tissues, whereas totipotent cells can differentiate into both embryonic and extraembryonic tissues. Currently, the ability to culture in vitro totipotent cells possessing molecular and functional features like those of an early embryo in vivo has been a challenge. Recently, it was reported that treatment with a single spliceosome inhibitor, pladienolide B (plaB), can successfully reprogram mouse pluripotent stem cells into totipotent blastomere-like cells (TBLCs) in vitro. The TBLCs exhibited totipotency transcriptionally and acquired expanded developmental potential with the ability to yield various embryonic and extraembryonic tissues that may be employed as novel mouse developmental cell models. However, it is disputed whether TBLCs are ‘true’ totipotent stem cells equivalent to in vivo two-cell stage embryos. To address this question, single-cell RNA sequencing was applied to TBLCs and cells from early mouse embryonic developmental stages and the data were integrated using canonical correlation analyses. Differential expression analyses were performed between TBLCs and multi-embryonic cell stages to identify differentially expressed genes. Remarkably, a subpopulation within the TBLCs population expressed a high level of the totipotent-related genes Zscan4s and displayed transcriptomic features similar to mouse two-cell stage embryonic cells. This study underscores the subtle differences between in vitro derived TBLCs and in vivo mouse early developmental cell stages at the single-cell transcriptomic level. Our study has identified a new experimental model for stem cell biology, namely ‘cluster 3’, as a subpopulation of TBLCs that can be molecularly defined as near totipotent cells.

scholarly journals Fluorescence enhanced BA-LIFT for single cell detection and isolation

2020 ◽  
Vol 12 (2) ◽  
pp. 025019 ◽  
Author(s):  
A Marquez ◽  
M Gómez-Fontela ◽  
S Lauzurica ◽  
R Candorcio-Simón ◽  
D Munoz-Martin ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Detection ◽  
Single Cell Detection

The Role of Nanotechnology in Single-Cell Detection: A Review

2014 ◽  
Vol 10 (10) ◽  
pp. 2598-2619 ◽  
Author(s):  
Changling Wang ◽  
Yuxiang Zhang ◽  
Mingdian Xia ◽  
Xingxi Zhu ◽  
Shitao Qi ◽  
...  
Keyword(s):  
Single Cell ◽  
Cell Detection ◽  
Single Cell Detection

Single-cell detection by cavity ring-down spectroscopy

2004 ◽  
Vol 85 (19) ◽  
pp. 4523 ◽  
Author(s):  
Peter B. Tarsa ◽  
Aislyn D. Wist ◽  
Paul Rabinowitz ◽  
Kevin K. Lehmann
Keyword(s):  
Single Cell ◽  
Cell Detection ◽  
Cavity Ring Down Spectroscopy ◽  
Single Cell Detection ◽  
Cavity Ring Down
Sign in / Sign up

Export Citation Format

Share Document