scholarly journals Detection of Coxiella burnetii Using Silicon Microring Resonator in Patient Blood Plasma

Micromachines ◽  
2019 ◽  
Vol 10 (7) ◽  
pp. 427 ◽  
Author(s):  
Bonhan Koo ◽  
Choong Eun Jin ◽  
Moonsuk Bae ◽  
Yoon Ok Jang ◽  
Ji Yeun Kim ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Blood Plasma ◽  
Coxiella Burnetii ◽  
Q Fever ◽  
Microring Resonator ◽  
Clinical Samples ◽  
Plasma Samples ◽  
Label Free ◽  
Highly Sensitive ◽  
Low Sensitivity

Blood plasma from patients is a powerful resource for diagnosing infectious disease due to it having many genetic materials as well as being relatively easy to obtain. Thus, various biosensors have been investigated for diagnosing diseases in blood plasma. However, there are no optimized and validated sensors for clinical use due to the low sensitivity, complexity, and difficulties of removing the inhibitors from plasma samples. In this study, we described a silicon microring resonator sensor used to detect Coxiella burnetii from the blood plasma of Q-fever patients in a label-free, real-time manner. Q-fever is an infectious disease caused by Coxiella burnetii via direct contact or inhalation aerosols. We validated this biosensor in the blood plasma of 35 clinical samples (including 16 Q fever samples infected with Coxiella burnetii and 19 samples infected with other febrile diseases. The biosensors are capable of rapid (10 min), highly sensitive (87.5%), and specific (89.5%) detection in plasma samples compared to the use of the conventional method.

A One Health Perspective on Q Fever

2019 ◽  
pp. 174-194
Author(s):  
Rita Cruz ◽  
Carmen Vasconcelos-Nobrega ◽  
Fernando Esteves ◽  
Catarina Coelho ◽  
Ana Sofia Ferreira ◽  
...  
Keyword(s):  
Public Health ◽  
Infectious Disease ◽  
Coxiella Burnetii ◽  
One Health ◽  
Q Fever ◽  
Human Infection ◽  
Sheep And Goats ◽  
Veterinary Public Health ◽  
Human Infections

Q fever is a worldwide zoonotic infectious disease caused by Coxiella burnetii and ruminants, namely, cattle, sheep, and goats, are known to be the main reservoir for human infection. C. burnetii infection in animals can result in epizootic abortions which are often associated with vast bacteria shedding in birth fluids and placentas. Human infections mainly occur in persons handling infected animals and their products. Here the authors describe the history, bacteriology, biosafety, and epidemiology of Q fever, now known to be a serious threat to veterinary public health.

OBSERVATIONS ON THE THERMAL INACTIVATION OF THE ORGANISM OF Q FEVER IN MILK.1

1956 ◽  
Vol 19 (11) ◽  
pp. 313-318 ◽  
Author(s):  
J. B. Enright ◽  
W. W. Sadler ◽  
R. C. Thomas
Keyword(s):  
Infectious Disease ◽  
Coxiella Burnetii ◽  
Thermal Inactivation ◽  
Q Fever ◽  
Minimum Standard ◽  
Sheep And Goats ◽  
Sources Of Infection ◽  
The Times

Q Fever is an infectious disease of man. Cattle, sheep and goats, who for the most part suffer inapparent infections with the organism, are the important sources of infection for man. These animals shed the organism in their milk. This manuscript reports on the cooperative studies designed to determine the times and temperatures needed to eliminate the causative rickettsiae, Coxiella burnetii, from cows milk. It is reported that the present minimum standard of pasteurization by the vat method of 143° F. for 30 minutes is inadequate, but the temperature of 145° F. for 30 minutes will eliminate the organism. The pasteurization of milk according to the present standards for HTST equipment of 161° F. for 15 seconds seems adequate to destroy C. burnetii.

scholarly journals High-Content Screening, a Reliable System for Coxiella burnetii Isolation from Clinical Samples

2020 ◽  
Vol 58 (5) ◽  
Author(s):  
Rania Francis ◽  
Maxime Mioulane ◽  
Marion Le Bideau ◽  
Marie-Charlotte Mati ◽  
Pierre-Edouard Fournier ◽  
...  
Keyword(s):  
Cell Culture ◽  
Coxiella Burnetii ◽  
Q Fever ◽  
Standard Procedure ◽  
Epidemiological Studies ◽  
High Content Screening ◽  
Clinical Samples ◽  
Content Type ◽  
Shell Vial ◽  
Level 3

ABSTRACT Q fever, caused by Coxiella burnetii, is a worldwide zoonotic disease that may cause severe forms in humans and requires a specific and prolonged antibiotic treatment. Although current serological and molecular detection tools allow a reliable diagnosis of the disease, culture of C. burnetii strains is mandatory to assess their susceptibility to antibiotics and sequence their genome in order to optimize patient management and epidemiological studies. However, cultivating this fastidious microorganism is difficult and restricted to reference centers, as it requires biosafety level 3 laboratories and relies on cell culture performed by experienced technicians. In addition, the culture yield is low, which results in a small number of isolates being available. In this work, we developed a novel high-content screening (HCS) isolation strategy based on optimized high-throughput cell culture and automated microscopic detection of infected cells with specifically designed algorithms targeting cytopathic effects. This method was more efficient than the shell vial assay, at the level of time dependency, when applied to both frozen specimens (7 isolates recovered by HCS only, sensitivity 91% versus 78% for shell vial) and fresh samples (1 additional isolate using HCS, sensitivity 7% versus 5% for shell vial), for which most strains were recovered more rapidly with the new technique. In addition, detecting positive cultures by an automated microscope reduced the need for expertise and saved 24% of technician working time. Application of HCS to antibiotic susceptibility testing of 12 strains demonstrated that it was as efficient as the standard procedure that combines shell vial culture and quantitative PCR.

scholarly journals Q Fever in the Greek Island of Crete: Detection, Isolation, and Molecular Identification of Eight Strains of Coxiella burnetii from Clinical Samples

1998 ◽  
Vol 36 (7) ◽  
pp. 2063-2067 ◽  
Author(s):  
Ioanna Spyridaki ◽  
Achilleas Gikas ◽  
Diamantis Kofteridis ◽  
Anna Psaroulaki ◽  
Yannis Tselentis
Keyword(s):  
Coxiella Burnetii ◽  
Nested Pcr ◽  
Q Fever ◽  
Buffy Coat ◽  
Clinical Samples ◽  
Polymorphism Analysis ◽  
Antibody Technique ◽  
Presenting Symptoms ◽  
Specificity And Sensitivity ◽  
Reference Strains

Over a period of 6 years (1989 to 1995), serum samples from 3,300 patients suspected to be infected by Coxiella burnetii were assayed for the presence of antibodies against antigen phase II of the microorganism by the indirect immunofluorescence antibody technique (IFAT). One hundred fifty-two cases were recorded, and blood samples from 17 patients were cultured for the isolation of the pathogen. By a centrifugation shell vial technique, eight strains were isolated from patients suffering from acute Q fever. The microorganism was detected in the cultures by IFAT, by Gimenez staining, and by the cytopathogenic effect on Vero and human embryonic lung (HEL) cells. PCR followed by restriction fragment length polymorphism analysis was used to confirm the diagnosis and identify the Coxiella burnetii strains within the cell cultures as well as to compare them with reference strains. In order to avoid time-consuming cultures, to achieve direct detection of Coxiella burnetii in clinical samples (blood, buffy coat, etc.), and to increase the specificity and sensitivity of the detection, nested PCR was performed. The first step of DNA extraction was performed with the QIAamp blood kit 250. For the second step of the PCR assays, the conditions of temperature and times of recycling were properly modified, and the microorganism was detected within 4 h. Our study demonstrates that Q fever is an endemic disease in Crete and that the diagnosis of Coxiella burnetii infection can be rapidly achieved by the detection of the microorganism in buffy coat samples by nested PCR. Although the presenting symptoms of the disease in this study differed from those in other studies, the Cretan strains do not differ genotypically from the reference strains (Nine Mile and Q212).

scholarly journals High Content Screening, a reliable system for Coxiella burnetii isolation from clinical samples

2019 ◽  
Author(s):  
Rania Francis ◽  
Maxime Mioulane ◽  
Marion Le Bideau ◽  
Marie-Charlotte Mati ◽  
Pierre-Edouard Fournier ◽  
...  
Keyword(s):  
Cell Culture ◽  
Coxiella Burnetii ◽  
High Throughput ◽  
Antibiotic Susceptibility ◽  
Q Fever ◽  
Standard Procedure ◽  
Epidemiological Studies ◽  
High Content Screening ◽  
Clinical Samples ◽  
Shell Vial

AbstractQ fever, caused by Coxiella burnetii, is a worldwide zoonotic disease that may cause severe forms in humans and requires a specific and prolonged antibiotic treatment. Although the current serological and molecular detection tools enable a reliable diagnosis of the disease, culture of C. burnetii strains is mandatory to evaluate their antibiotic susceptibility and sequence their genome in order to optimize patient management and epidemiological studies. However, cultivating this fastidious microorganism is difficult and restricted to reference centers as it requires biosafety-level 3 laboratories and relies on cell culture performed by experienced technicians. In addition, the culture yield is low, which results in a small number of isolates being available. In this work, we developed a novel high content screening (HCS) isolation strategy based on optimized high-throughput cell culture and automated microscopic detection of infected cells with specifically-designed algorithms targeting cytopathic effects. This method was more efficient than the shell-vial assay when applied to both frozen specimens (7 isolates recovered by HCS only, sensitivity 91% vs 78% for shell-vial) and fresh samples (1 additional isolate using HCS, sensitivity 7% vs 5% for shell-vial). In addition, detecting positive cultures by an automated microscope reduced the need for expertise and saved 24% of technician working time. Application of HCS to antibiotic susceptibility testing of 12 strains demonstrated that it was as efficient as the standard procedure that combines shell-vial culture and quantitative PCR. Overall, this high-throughput HCS system paves the way to the development of improved cell culture isolation of human viruses.

scholarly journals Label-Free Plasmonic Biosensor for Rapid, Quantitative, and Highly Sensitive COVID-19 Serology: Implementation and Clinical Validation

2021 ◽  
Author(s):  
Olalla Calvo-Lozano ◽  
MIquel Sierra ◽  
Maria Soler ◽  
M.-Carmen Estevez ◽  
luis Chiscano-camon ◽  
...  
Keyword(s):  
Polyclonal Antibodies ◽  
Simultaneous Detection ◽  
Clinical Samples ◽  
Clinical Validation ◽  
Label Free ◽  
Serological Tests ◽  
N Protein ◽  
Lateral Flow Assays ◽  
Low Sensitivity ◽  
Biosensor Device

Serological tests are essential for the control and management of COVID-19 pandemic, not only for current and historical diagnostics but especially for surveillance, epidemiological, and acquired immunity studies. Clinical COVID-19 serology is routinely performed by enzymatic or chemiluminescence immunoassays (i.e., ELISA or CLIA), which provide good sensitivities at the expense of relatively long turnaround times and specialized laboratory settings. Rapid serological tests, based on lateral flow assays, have also been developed and widely commercialized, but they suffer from limited reliability due to relatively low sensitivity and specificity. We have developed and validated a direct serological biosensor assay employing proprietary technology based on Surface Plasmon Resonance (SPR). The biosensor offers a rapid -less than 15 min- identification and quantification of SARS-CoV-2 antibodies directly in clinical samples, without the need of any signal amplification. The portable plasmonic biosensor device employs a custom-designed multi-antigen sensor biochip, combining the two main viral antigens (RBD peptide and N protein), for simultaneous detection of human antibodies targeting both antigens. The SPR serology assay reaches detection limits in the low ng mL-1 range employing polyclonal antibodies as standard, which are well below the commonly detected antibody levels in COVID-19 patients. The assay has also been implemented employing the first WHO approved anti-SARS-CoV-2 immunoglobulin standard. We have carried out a clinical validation with COVID-19 positive and negative samples (n=120) that demonstrates the excellent diagnostic sensitivity (99%) and specificity (100%). This positions our biosensor device as an accurate, robust, and easy-to-use diagnostics tool for rapid and reliable COVID-19 serology to be employed both at laboratory and decentralized settings for the management of COVID-19 patients and for the evaluation of immunological status during vaccination, treatment or in front of emerging variants.<br>

scholarly journals SPR Biosensor for Quantification of Fetuin-A as a Promising Multibiomarker

2018 ◽  
pp. S367-S375 ◽  
Author(s):  
Z. RIEDELOVÁ ◽  
P. MÁJEK ◽  
K. PEČÁNKOVÁ ◽  
J. KUČEROVÁ ◽  
F. SURMAN ◽  
...  
Keyword(s):  
Blood Plasma ◽  
Binding Capacity ◽  
Point Of Care ◽  
Specific Antigen ◽  
Polymer Brush ◽  
Life Quality ◽  
Plasma Samples ◽  
Label Free ◽  
Fetuin A ◽  
Technological Advances

Early diagnosis of ongoing malignant disease is crucial to improve survival rate and life quality of the patients and requires sensitive detection of specific biomarkers e.g. prostate-specific antigen (PSA), carcinoembryonic antigen (CEA), alpha-fetoprotein (AFP), etc. In spite of current technological advances, malignant diseases are still identified in rather late stages, which have detrimental effect on the prognosis and treatment of the disease. Here, we present a biosensor able to detect fetuin-A, a potential multibiomarker. The biosensing platform is based on polymer brush combining antifouling monomer units of N-(2-hydroxypropyl)methacrylamide (HPMA) and carboxybetaine methacrylamide (CBMAA), statistically copolymerized by surface-initiated atom transfer radical polymerization. The copolymer poly(HPMA-co-CBMAA) exhibits excellent non-fouling properties in the most relevant biological media (i.e. blood plasma) as well as antithrombogenic surface properties by preventing the adhesion of blood components (i.e. leukocytes; platelets; and erythrocytes). Moreover, the polymer brush can be easily functionalized with biorecognition elements maintaining high resistance to blood fouling and the binding capacity can be regulated by tuning the ratio between CBMAA and HPMA units. The superior antifouling properties of the copolymer even after biofunctionalization were exploited to fabricate a new plasmonic biosensor for the analysis of fetuin-A in real clinical blood plasma samples. The assay used in this work can be explored as label-free affinity biosensor for diagnostics of different biomarkers in real clinical plasma samples and to shift the early biomarker detection toward novel biosensor technologies allowing point of care analysis.

scholarly journals A LINK BETWEEN ACTIVE MYELOPEROXIDASE AND CHLORINATED CERULOPLASMIN IN BLOOD PLASMA OF PATIENTS WITH CARDIOVASCULAR DISEASES

2018 ◽  
Vol 20 (5) ◽  
pp. 699-710 ◽  
Author(s):  
A. V. Sokolov ◽  
V. A. Kostevich ◽  
N. V. Gorbunov ◽  
D. V. Grigorieva ◽  
I. V. Gorudko ◽  
...  
Keyword(s):  
Cardiovascular Diseases ◽  
Blood Plasma ◽  
Hypochlorous Acid ◽  
Solid Phase ◽  
Antibody Activity ◽  
Myeloperoxidase Activity ◽  
Plasma Samples ◽  
Specific Product ◽  
Highly Sensitive ◽  
In Blood Plasma

Myeloperoxidase is a key factor promoting development of halogenative/oxidative stress under inflammatory conditions. Previously, we have discovered complexes including myeloperoxidase and its physiological inhibitor, ceruloplasmin in blood plasma of patients with inflammatory diseases of different etiology, e.g., atherosclerosis. Studies on regulation of myeloperoxidase activity by ceruloplasmin have shown that hypochlorous acid, a specific product of myeloperoxidase action, is likely to modify ceruloplasmin during inflammation. The present study was aimed for analysis of relationships between the myeloperoxidase activity, native, and HOCl-modified ceruloplasmin levels in blood plasma samples of the patients with cardiovascular diseases.Specific antibodies against myeloperoxidase, ceruloplasmin, and HOCl-modified ceruloplasmin were obtained and specific enzyme-linked immunosorbent assays (ELISA) were developed. A combination of highly sensitive methods of myeloperoxidase assay i.e., solid-phase adsorption of antigens with subsequent testing of either their activity, or peroxidase-labeled antibody activity allowed elaborating the highly sensitive assays for ceruloplasmin and its HOCl-modified molecules, and for myeloperoxidase (concentration, peroxidase and halogenating activity). Positive correlation was proven between the myeloperoxidase concentration and activities. HOCl-modified ceruloplasmin content also correlated with myeloperoxidase activity.The HOCl-modified ceruloplasmin was first discovered in blood plasma samples from patients with cardiovascular diseases. In view of correlation between myeloperoxidase activity and HOCl-modified ceruloplasmin content in plasma, we suggest that HOCl production is aimed for suppression of myeloperoxidaseinhibitory function of ceruloplasmin.

scholarly journals From autoimmune hepatitis to Q fever

2015 ◽  
Vol 10 (1) ◽  
pp. 58
Author(s):  
Davide Tizzani ◽  
Silvia Totaro ◽  
Valentina Laura Crudo ◽  
Andrea Viola ◽  
Vittorio Gallo ◽  
...  
Keyword(s):  
Infectious Disease ◽  
Liver Biopsy ◽  
Autoimmune Hepatitis ◽  
Medical History ◽  
Coxiella Burnetii ◽  
Fever Of Unknown Origin ◽  
Clinical Presentation ◽  
Q Fever ◽  
Unknown Origin ◽  
Granulomatous Hepatitis

Q fever is an infectious disease caused by <em>Coxiella burnetii.</em> Its clinical presentation is often nonspecific and the serological diagnosis difficult to make, especially in the absence of specific and suspected medical history. This article presents a case of fever of unknown origin (FUO), interpreted as an autoimmune hepatitis, later proven by the liver biopsy to be a granulomatous hepatitis caused by <em>C. burnetii</em>. The approach to FUO, the features of granulomatous hepatitis and Q fever are presented and discussed.

Sign in / Sign up

Export Citation Format

Share Document