scholarly journals A Continuous Flow-through Microfluidic Device for Electrical Lysis of Cells

Micromachines ◽  
2019 ◽  
Vol 10 (4) ◽  
pp. 247 ◽  
Author(s):  
Ying-Jie Lo ◽  
U Lei
Keyword(s):  
Electric Field ◽  
Cross Flow ◽  
Electrode Array ◽  
Threshold Value ◽  
Lower Half ◽  
Rectangular Microchannel ◽  
Peak Voltage ◽  
Biomedical Systems ◽  
Electrical Lysis ◽  
Flow Through

In contrast to the delicate 3D electrodes in the literature, a simple flow-through device is proposed here for continuous and massive lysis of cells using electricity. The device is essentially a rectangular microchannel with a planar electrode array built on its bottom wall, actuated by alternating current (AC) voltages between neighboring electrodes, and can be incorporated easily into other biomedical systems. Human whole blood diluted 10 times with phosphate-buffered saline (about 6 × 108 cells per mL) was pumped through the device, and the cells were completely lysed within 7 s after the application of a 20 V peak-to-peak voltage at 1 MHz, up to 400 μL/hr. Electric field and Maxwell stress were calculated for assessing electrical lysis. Only the lower half-channel was exposed to an electric field exceeding the irreversible threshold value of cell electroporation (Eth2), suggesting that a cross flow, proposed here primarily as the electro-thermally induced flow, was responsible for bringing the cells in the upper half-channel downward to the lower half-channel. The Maxwell shear stress associated with Eth2 was one order of magnitude less than the threshold mechanical stresses for lysis, implying that an applied moderate mechanical stress could aid electrical lysis.

Surface-conduction enhanced dielectrophoretic-like particle migration in electric-field driven fluid flow through a straight rectangular microchannel

2017 ◽  
Vol 29 (10) ◽  
pp. 102001 ◽  
Author(s):  
Zhijian Liu ◽  
Di Li ◽  
Yongxin Song ◽  
Xinxiang Pan ◽  
Dongqing Li ◽  
...  
Keyword(s):  
Fluid Flow ◽  
Electric Field ◽  
Particle Migration ◽  
Rectangular Microchannel ◽  
Flow Through ◽  
Surface Conduction

scholarly journals Trade-Off between Operating Time and Energy Consumption in Pulsed Electric Field Electrodialysis: A Comprehensive Simulation Study

Membranes ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. 43
Author(s):  
Manuel César Martí-Calatayud ◽  
Mario Sancho-Cirer Poczatek ◽  
Valentín Pérez-Herranz
Keyword(s):  
Energy Consumption ◽  
Electric Field ◽  
Simulation Study ◽  
Specific Energy ◽  
Operating Time ◽  
Specific Energy Consumption ◽  
Threshold Value ◽  
Pulsed Electric Field ◽  
Pulse Frequency ◽  
Time And Energy

Electrodialysis (ED) has been recently introduced in a variety of processes where the recovery of valuable resources is needed; thus, enabling sustainable production routes for a circular economy. However, new applications of ED require optimized operating modes ensuring low energy consumptions. The application of pulsed electric field (PEF) electrodialysis has been demonstrated to be an effective option to modulate concentration polarization and reduce energy consumption in ED systems, but the savings in energy are usually attained by extending the operating time. In the present work, we conduct a comprehensive simulation study about the effects of PEF signal parameters on the time and energy consumption associated with ED processes. Ion transport of NaCl solutions through homogeneous cation-exchange membranes is simulated using a 1-D model solved by a finite-difference method. Increasing the pulse frequency up to a threshold value is effective in reducing the specific energy consumption, with threshold frequencies increasing with the applied current density. Varying the duty cycle causes opposed effects in the time and energy usage needed for a given ED operation. More interestingly, a new mode of PEF functions with the application of low values of current during the relaxation phases has been investigated. This novel PEF strategy has been demonstrated to simultaneously improve the time and the specific energy consumption of ED processes.

Mechanism of Cell Lysis in Microfluidic Channel With Integrated Nanocomposite Electrodes

2013 ◽  
Author(s):  
Madhusmita Mishra ◽  
Anil Krishna Koduri ◽  
Aman Chandra ◽  
D. Roy Mahapatra ◽  
G. M. Hegde
Keyword(s):  
Electric Field ◽  
Microfluidic Channel ◽  
Cell Lysis ◽  
Bacterial Cells ◽  
Nano Composite ◽  
Time Resolved ◽  
Ac Electric Field ◽  
Cell Dispersion ◽  
Electrical Lysis ◽  
Nanocomposite Electrodes

This paper reports on the characterization of an integrated micro-fluidic platform for controlled electrical lysis of biological cells and subsequent extraction of intracellular biomolecules. The proposed methodology is capable of high throughput electrical cell lysis facilitated by nano-composite coated electrodes. The nano-composites are synthesized using Carbon Nanotube and ZnO nanorod dispersion in polymer. Bacterial cells are used to demonstrate the lysis performance of these nanocomposite electrodes. Investigation of electrical lysis in the microchannel is carried out under different parameters, one with continuous DC application and the other under DC biased AC electric field. Lysis in DC field is dependent on optimal field strength and governed by the cell type. By introducing the AC electrical field, the electrokinetics is controlled to prevent cell clogging in the micro-channel and ensure uniform cell dispersion and lysis. Lysis mechanism is analyzed with time-resolved fluorescence imaging which reveal the time scale of electrical lysis and explain the dynamic behavior of GFP-expressing E. coli cells under the electric field induced by nanocomposite electrodes. The DNA and protein samples extracted after lysis are compared with those obtained from a conventional chemical lysis method by using a UV–Visible spectroscopy and fluorimetry. The paper also focuses on the mechanistic understanding of the nano-composite coating material and the film thickness on the leakage charge densities which lead to differential lysis efficiency.

Wall Boundary Layers in Turbomachines

1972 ◽  
Vol 14 (6) ◽  
pp. 411-423 ◽  
Author(s):  
H. Marsh ◽  
J. H. Horlock
Keyword(s):  
Boundary Layer ◽  
Integral Equations ◽  
Cross Flow ◽  
Inlet Guide Vanes ◽  
Wall Boundary Layer ◽  
Wall Boundary ◽  
Alternative Approach ◽  
Flow Through ◽  
The Many ◽  
Momentum Integral

Equations for the passage-averaged flow in a cascade are used to derive the momentum integral equations governing the development of the wall boundary layer in turbomachines. Several existing methods of analysis are discussed and an alternative approach is given which is based on the passage-averaged momentum integral equations. The analysis leads to an anomaly in the prediction of the cross flow and to avoid this it is suggested that for the many-bladed cascade there should be a variation of the blade force through the boundary layer. This variation of the blade force can be included in the analysis as a force deficit integral. The growth of the wall boundary layer has been calculated by four methods and the predictions are compared with two sets of published experimental results for flow through inlet guide vanes.

scholarly journals Design and development of a multichannel potentiometer for monitoring an electrode array and its application in flow analysis

2002 ◽  
Vol 24 (4) ◽  
pp. 105-110 ◽  
Author(s):  
Jarbas J. R. Rohwedder ◽  
Celio Pasquini ◽  
Ivo M. Raimundo, Jr. ◽  
M. Conceiçao ◽  
B. S. M. Montenegro ◽  
...  
Keyword(s):  
Standard Deviation ◽  
Flow Analysis ◽  
Reference Electrode ◽  
Electrode Array ◽  
Ion Selective Electrodes ◽  
Electrode Arrays ◽  
Figures Of Merit ◽  
Flow Through ◽  
The Individual ◽  
Intense Signal

A versatile potentiometer that works with electrode arrays in flow injection and/or monosegmented flow systems is described. The potentiometer is controlled by a microcomputer that allows individual, sequential multiplexed or random accesses to eight electrodes while employing only one reference electrode. The instrument was demonstrated by monitoring an array of seven flow-through ion-selective electrodes for Ag+and for three electrodes for Cl-, Ca2+and K+. The figures of merit of the individual and multiplexed (summed) readings of the electrode array were compared. The absolute standard deviation of the measurements made by summing the potential of two or more electrodes was maintained constant, thus improving the precision of the measurements. This result shows that an attempt to combine the signals of the electrodes to produce a more intense signal in the Hadamard strategy is feasible and accompanied by a proportional improvement in the precision of individual measurements. The preliminary tests suggest that the system can allow for 270 determinations per hour, with a linear range from1.0×10−2to1.0×10−4mol l-1for the three di¡erent analytes. Detection limits were estimated as3.1×10−5,3.0×10−6and1.0×10−5mol l-1for Cl-, Ca2+and K+, respectively.

scholarly journals Integration of marker-free selection of single cells at a wireless electrode array with parallel fluidic isolation and electrical lysis

2019 ◽  
Vol 10 (5) ◽  
pp. 1506-1513 ◽  
Author(s):  
Min Li ◽  
Robbyn K. Anand
Keyword(s):  
Single Cell ◽  
Single Cells ◽  
Electrode Array ◽  
Cell Capture ◽  
Bipolar Electrodes ◽  
Manual Intervention ◽  
Electrical Lysis ◽  
Marker Free ◽  
Selection Of

We present integration of selective single-cell capture at an array of wireless electrodes (bipolar electrodes, BPEs) with transfer into chambers, reagent exchange, fluidic isolation and rapid electrical lysis in a single platform, thus minimizing sample loss and manual intervention steps.

Control of Dielectric Fluid Flow Distribution in Micro-Tubes With EHD Conduction Pumping: Numerical Study

2011 ◽  
Author(s):  
Miad Yazdani ◽  
Jamal Seyed-Yagoobi
Keyword(s):  
Fluid Flow ◽  
Electric Field ◽  
Conduction Mechanism ◽  
Numerical Study ◽  
Fluid Motion ◽  
Flow Distribution ◽  
Operating Conditions ◽  
Dielectric Fluid ◽  
Total Flow ◽  
Flow Through

The control of fluid flow distribution in micro-scale tubes is numerically investigated. The flow distribution control is achieved via electric conduction mechanism. In electrohydrodynamic (EHD) conduction pumping, when an electric field is applied to a fluid, dissociation and recombination of electrolytic species produces heterocharge layers in the vicinity of electrodes. Attraction between electrodes and heterocharge layers induces a fluid motion and a net flow is generated if the electrodes are asymmetric. The numerical domain comprises a 2-D manifold attached to two bifurcated tubes with one of the tubes equipped with a bank of uniquely designed EHD-conduction electrodes. In the absence of electric field, the total flow supplied at the manifold’s inlet is equally distributed among the tubes. The EHD-conduction, however, operates as a mechanism to manipulate the flow distribution to allow the flow through one branch surpasses the counterpart of the other branch. Its performance is evaluated under various operating conditions.

THE FREQUENCY DEPENDENCE AND NON-LINEAR DIELECTRIC PROPERTY OF ER FLUID

1996 ◽  
Vol 10 (23n24) ◽  
pp. 3073-3080 ◽  
Author(s):  
KUNQUAN LU ◽  
WEIJIA WEN ◽  
CHENXI LI
Keyword(s):  
Dielectric Property ◽  
Field Strength ◽  
Electric Field ◽  
Frequency Dependence ◽  
Threshold Value ◽  
Shear Stresses ◽  
Linear Behavior ◽  
Non Linear ◽  
Er Fluids ◽  
Er Fluid

The frequency dependence of the shear stress in ac field and the non-linear dielectric property of ER fluid have been studied. We find that the shear stresses of some water-free ER fluids increase monotonously with the frequency and tend to reach saturated values at high frequency. The measurements on KNbO 3/silicone ER fluid show that the shear stresses under 103 Hz frequency a.c. field are several times or even an order larger than that under d.c. field for the same field strength. The studies of non-linear dielectric properties of ER fluids show that the permittivity of ER fluid increases linearly with increasing field strength when the electric field exceeds a threshold value E 1 and tends to a saturated constant beyond a high field strength E 2. Correspondingly the current density follows linear behavior no longer in the region between E 1 and E 2. A model based on the rearrangement of the particles under the electric field. which causes the variation of the dielectric property of the ER fluid, is proposed and the analysis is consistent with the measured results.

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