scholarly journals Detection of Hepatitis C Virus Core Protein in Serum Using Aptamer-Functionalized AFM Chips

Micromachines ◽  
2019 ◽  
Vol 10 (2) ◽  
pp. 129 ◽  
Author(s):  
Tatyana Pleshakova ◽  
Anna Kaysheva ◽  
Ivan Shumov ◽  
Vadim Ziborov ◽  
Jana Bayzyanova ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Core Protein ◽  
Protein Detection ◽  
Core Antigen ◽  
Serum Samples ◽  
Chip Surface ◽  
Force Microscopy ◽  
Virus Core ◽  
Complex Protein

In the present study, we demonstrate atomic force microscopy (AFM)-based detection of hepatitis C virus (HCV) particles in serum samples using a chip with aptamer-functionalized surface (apta-based AFM chip). The target particles, containing core antigen of HCV (HCVcoreAg protein), were biospecifically captured onto the chip surface from 1 mL of test solution containing 10 µL of serum collected from a hepatitis C patient. The registration of aptamer/antigen complexes on the chip surface was performed by AFM. The aptamers used in the present study were initially developed for therapeutic purposes; herein, these aptamers have been successfully utilized as probe molecules for HCVcoreAg detection in the presence of a complex protein matrix (human serum). The results obtained herein can be used for the development of detection systems that employ affine enrichment for protein detection.

scholarly journals Detection of hepatitis C virus core protein in serum by atomic force microscopy combined with mass spectrometry

2015 ◽  
pp. 1597 ◽  
Author(s):  
Ivan Shumov ◽  
Yuri Ivanov ◽  
Anna Kaysheva ◽  
Pavel Frantsuzov ◽  
Tatyana Pleshakova ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Atomic Force Microscopy ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Core Protein ◽  
Force Microscopy ◽  
Virus Core ◽  
Atomic Force

scholarly journals Hepatitis C Virus Core Mutations Associated with False-Negative Serological Results for Genotype 3a Core Antigen

2015 ◽  
Vol 53 (8) ◽  
pp. 2697-2700 ◽  
Author(s):  
Linh Thuy Nguyen ◽  
Linda Dunford ◽  
Ines Freitas ◽  
Paul Holder ◽  
Lan Anh Nguyen ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Low Income ◽  
Core Protein ◽  
False Negative ◽  
Core Antigen ◽  
Virus Core ◽  
Genotype 3A ◽  
Positive Controls

Genetic characterization of the genotype 3a (GT3a) hepatitis C virus (HCV)coreregion from HCV core antigen (HCVcAg)-negative/RNA-positive cases and HCVcAg-positive/RNA-positive controls identified significant associations between the substitutions A48T and T49A/P and failure to detect HCVcAg (P< 0.05). Polymorphisms at residues 48 and 49 in the core protein are present across all major epidemic and endemic GTs. These findings have implications for HCV diagnosis, particularly in low-income regions in which GT3a HCV is endemic.

Hepatitis C virus core protein detection by DNA aptamer

2011 ◽  
Vol 41 (8) ◽  
pp. 1312-1318 ◽  
Author(s):  
Zhen ZHANG ◽  
HaiZhen ZHU ◽  
WeiHong TAN ◽  
ZiLong ZHAO ◽  
XiaoHong FANG ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Core Protein ◽  
Protein Detection ◽  
Dna Aptamer ◽  
Virus Core

Characterization of Hepatitis C Virus Core Protein Dimerization by Atomic Force Microscopy

2018 ◽  
Vol 90 (7) ◽  
pp. 4596-4602 ◽  
Author(s):  
Wenhui Li ◽  
Xiaolong Kou ◽  
Jiachao Xu ◽  
Wei Zhou ◽  
Rong Zhao ◽  
...  
Keyword(s):  
Atomic Force Microscopy ◽  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Core Protein ◽  
Protein Dimerization ◽  
Force Microscopy ◽  
Virus Core ◽  
Atomic Force

scholarly journals Nanowire Aptamer-Sensitized Biosensor Chips with Gas Plasma-Treated Surface for the Detection of Hepatitis C Virus Core Antigen

Coatings ◽  
2020 ◽  
Vol 10 (8) ◽  
pp. 753 ◽  
Author(s):  
Kristina A. Malsagova ◽  
Tatyana O. Pleshakova ◽  
Rafael A. Galiullin ◽  
Ivan D. Shumov ◽  
Andrey F. Kozlov ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Silicon On Insulator ◽  
Core Antigen ◽  
Response Signal ◽  
Serum Samples ◽  
Buffer Solution ◽  
Protein Marker ◽  
Virus Core ◽  
Gas Plasma

Herein, we have demonstrated highly sensitive real-time biospecific detection of a protein marker of hepatitis C—the core antigen of hepatitis C virus (HCVcoreAg)—using a nanowire (NW) biosensor. The primary element of the NW-biosensor is a chip with p-type conductance, bearing silicon-on-insulator (SOI) nanowire structures on its surface. The nanowire structures are fabricated by gas-plasma treatment and electron beam lithography. The detection specificity was provided by sensitization of the sensor surface with aptamers against HCVcoreAg. The influence of buffer pH on the sensor response signal was studied. The effect of reverse polarity of the biosensor response signal with change from the acidic buffer pH to the neutral one was found. The lowest detectable HCVcoreAg concentration was determined to be 2.0 × 10−15 M in both acidic (pH 5.1) and neutral (pH 7.4) buffer solution. The proposed aptamer-sensitized sensor was also successfully applied to detect HCVcoreAg in serum samples of hepatitis C patients.

Role of Hepatitis C Virus Core Antigen Assay in Blood Donors Screening at Zagazig University Hospitals

2018 ◽  
Vol 8 (1) ◽  
pp. 48-54
Author(s):  
Rashed Hassan ◽  
Abdelmonem Elshamy ◽  
Sameh Abdel Monem ◽  
Emad Moustafa ◽  
Essam Wahab
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Blood Donors ◽  
Core Antigen ◽  
University Hospitals ◽  
Virus Core ◽  
Antigen Assay

Biosynthesis and biochemical properties of the hepatitis C virus core protein.

1994 ◽  
Vol 68 (6) ◽  
pp. 3631-3641 ◽  
Author(s):  
E Santolini ◽  
G Migliaccio ◽  
N La Monica
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Core Protein ◽  
Biochemical Properties ◽  
Virus Core

Truncated hepatitis C virus core protein encoded in hepatocellular carcinomas

2004 ◽  
Author(s):  
Rin Yamaguchi ◽  
Seiya Momosaki ◽  
Guang Gao ◽  
Chu Hsia ◽  
Masamichi Kojiro ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Core Protein ◽  
Virus Core ◽  
Hepatocellular Carcinomas
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