scholarly journals HPLC-DAD-ESI-QTOF-MS Determination of Bioactive Compounds and Antioxidant Activity Comparison of the Hydroalcoholic and Water Extracts from Two Helichrysum italicum Species

Metabolites ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 403
Author(s):  
Katja Kramberger ◽  
Darja Barlič-Maganja ◽  
Dunja Bandelj ◽  
Alenka Baruca Arbeiter ◽  
Kelly Peeters ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
High Performance ◽  
Extraction Procedure ◽  
Metabolite Profile ◽  
Extraction Process ◽  
Hot Water ◽  
Total Phenolic ◽  
Water Extracts ◽  
Flight Mass Spectrometry ◽  
Functional Beverages

Mediterranean plant Helichrysum italicum represents a rich source of versatile bioactive compounds with potential benefits for human health. Despite extensive research on the plant’s active constituents, little attention has yet been paid to characterizing the relationship between its intra-specific genetic diversity and metabolite profile. The study aimed to determine metabolic profile of H. italicum ssp. italicum (HII) and ssp. tyrrhenicum (HIT) cultivated on the experimental plantation in Slovenia and to compare the chemical composition of extracts regarding the solvent extraction process. Extracts were prepared upon conventional extract preparation procedures: maceration with 50% methanol or ethanol and cold or hot water infusion and analyzed using High Performance Liquid Chromatography-Diode Array Detection-Electrospray Ionization-Quadrupole Time-of-Flight-Mass Spectrometry (HPLC-DAD-ESI-QTOF-MS). One hundred compounds were identified in the samples, among them several isomers and derivatives were reported for the first time, while caffeoylquinic acids and pyrones were the most abundant. Semi-quantitative comparison revealed that the extraction procedure had a greater impact on the chemical profile than genetic variability. All HIT extracts showed a higher total phenolic content compared to HII, while the antioxidant potential evaluated by 1,1-diphenyl-2-picrylhydrazil test was not proportionally higher. In addition, hot water extracts proved to be comparably active as alcoholic ones, confirming high commercial potential of Helichrysum italicum as herbal functional beverages.

scholarly journals Optimization of Total Phenolic and Flavonoid Contents of Defatted Pitaya (Hylocereus polyrhizus) Seed Extract and Its Antioxidant Properties

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 787 ◽  
Author(s):  
Siti Atikah Zulkifli ◽  
Siti Salwa Abd Gani ◽  
Uswatun Hasanah Zaidan ◽  
Mohd Izuan Effendi Halmi
Keyword(s):  
High Performance ◽  
Ethanol Concentration ◽  
Antioxidant Properties ◽  
Extraction Process ◽  
Seed Extract ◽  
Extraction Time ◽  
Total Phenolic ◽  
Extraction Temperature ◽  
Antioxidant Power ◽  
Flight Mass Spectrometry

The present study was conducted to optimize extraction process for defatted pitaya seed extract (DPSE) adopting response surface methodology (RSM). A five-level central composite design was used to optimize total phenolic content (TPC), total flavonoid content (TFC), ferric reducing antioxidant power (FRAP), and 2,2′-azino-bis (3-ethylbenzothizoline-6-sulfonic acid (ABTS) activities. The independent variables included extraction time (30–60 min), extraction temperature (40–80 °C) and ethanol concentration (60%–80%). Results showed that the quadratic polynomial equations for all models were significant at (p < 0.05), with non-significant lack of fit at p > 0.05 and R2 of more than 0.90. The optimized extraction parameters were established as follows: extraction time of 45 min, extraction temperature of 70 °C and ethanol concentration of 80%. Under these conditions, the recovery of TPC, TFC, and antioxidant activity based on FRAP and ABTS were 128.58 ± 1.61 mg gallic acid equivalent (GAE)/g sample, 9.805 ± 0.69 mg quercetin equivalent (QE)/g sample, 1.23 ± 0.03 mM Fe2+/g sample, and 91.62% ± 0.15, respectively. Ultra-high-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC-QTOF/MS) analysis identified seven chemical compounds with flavonoids constituting major composition of the DPSE.

scholarly journals Untargeted Metabolomics of Korean Fermented Brown Rice Using UHPLC Q-TOF MS/MS Reveal an Abundance of Potential Dietary Antioxidative and Stress-Reducing Compounds

Antioxidants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 626
Author(s):  
Akanksha Tyagi ◽  
Su-Jung Yeon ◽  
Eric Banan-Mwine Daliri ◽  
Xiuqin Chen ◽  
Ramachandran Chelliah ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
High Performance ◽  
Antioxidant Activities ◽  
Brown Rice ◽  
Dry Weight ◽  
Untargeted Metabolomics ◽  
Total Phenolic ◽  
Germinated Brown Rice ◽  
Antioxidant Power ◽  
Flight Mass Spectrometry

Free radical-induced oxidative stress is the root cause of many diseases, such as diabetes, stress and cardiovascular diseases. The objective of this research was to screen GABA levels, antioxidant activities and bioactive compounds in brown rice. In this study, we first fermented brown rice with different lactic acid bacteria (LABs), and the best LAB was selected based on the levels of GABA in the fermentate. Lactobacillus reuterii generated the highest levels of GABA after fermentation. To ascertain whether germination can improve the GABA levels of brown rice, we compared the levels of GABA in raw brown rice (Raw), germinated brown rice (Germ), fermented brown rice (Ferm) and fermented-germinated brown rice (G+F) to identify the best approach. Then, antioxidant activities were investigated for Raw BR, Germ BR, Ferm BR and G+F BR. Antioxidant activity was calculated using a 2,2-diphenyl-1-picryl hydrazile radical assay, 2,2-azino-bis-(3-ethylene benzothiozoline-6-sulfonic acid) radical assay and ferric-reducing antioxidant power. In Ferm BR, DPPH (114.40 ± 0.66), ABTS (130.52 ± 0.97) and FRAP (111.16 ± 1.83) mg Trolox equivalent 100 g, dry weight (DW), were observed as the highest among all samples. Total phenolic content (97.13 ± 0.59) and total flavonoids contents (79.62 ± 1.33) mg GAE/100 g and catechin equivalent/100 g, DW, were also found to be highest in fermented BR. Furthermore, an untargeted metabolomics approach using ultra-high-performance liquid tandem chromatography quadrupole time of flight mass spectrometry revealed the abundance of bioactive compounds in fermented BR, such as GABA, tryptophan, coumaric acid, L-ascorbic acid, linoleic acid, β-carotenol, eugenol, 6-gingerol, etc., as well as bioactive peptides which could contribute to the health-promoting properties of L. reuterii fermented brown rice.

scholarly journals Seasonal Variation of Health-Promoting Bioactives in Broccoli and Methyl-Jasmonate Pre-Harvest Treatments to Enhance Their Contents

Foods ◽  
2020 ◽  
Vol 9 (10) ◽  
pp. 1371 ◽  
Author(s):  
Vanesa Nuñez-Gómez ◽  
Nieves Baenas ◽  
Inma Navarro-González ◽  
Javier García-Alonso ◽  
Diego A. Moreno ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Phenolic Compounds ◽  
Methyl Jasmonate ◽  
Bioactive Compounds ◽  
High Performance ◽  
Total Phenolic ◽  
Diode Array ◽  
Total Carotenoids ◽  
Health Promoting

Broccoli is a source of bioactive compounds that provide an important nutritional value. The content of these compounds can vary depending on agronomic and environmental conditions, as well as on elicitation. In this study, three crop trials were carried out to evaluate the effects of the cultivation season, the application of different dosages of methyl-jasmonate (MeJA) on the overall quality and on the total content of bioactive compounds of ‘Parthenon’ broccoli cultivated under the field conditions of southeastern Spain. Color parameters, chlorophyll content, total phenolic compounds, total flavonoids and antioxidant activity were measured to evaluate the overall quality. Moreover, individual carotenoids, phenolic compounds and glucosinolates were evaluated by high performance liquid chromatography with diode array detection (HPLC-DAD) and high performance liquid chromatography equipped with diode array detector coupled to mass spectrometer using electro spray ionization (HPLC-DAD-ESI/MSn). The content of total carotenoids, phenolic compounds and glucosinolates were higher in autumn compared with spring, showing increases of 2.8-fold, 2-fold and 1.2-fold, respectively. Moreover, a double application of MeJA increased the contents of total carotenoids, phenolic compounds and glucosinolates by 22%, 32% and 39%, respectively, relative to the untreated samples. Considering our results, the controlled and timely application of 250 µM MeJA to the aerial parts of the plants four days before harvest, on two consecutive days, seems to be a valid agronomic strategy to improve the health-promoting capacity of Parthenon broccoli, without compromising its overall quality.

scholarly journals Acylated Flavonoid Glycosides are the Main Pigments that Determine the Flower Colour of the Brazilian Native Tree Tibouchina pulchra (Cham.) Cogn.

Molecules ◽  
2019 ◽  
Vol 24 (4) ◽  
pp. 718 ◽  
Author(s):  
Fernanda Rezende ◽  
Marcelo Ferreira ◽  
Mads Clausen ◽  
Magdalena Rossi ◽  
Claudia Furlan
Keyword(s):  
Liquid Chromatography ◽  
High Performance ◽  
Flower Colour ◽  
Metabolite Profile ◽  
Ultra Performance Liquid Chromatography ◽  
Flavonoid Glycosides ◽  
Array Detector ◽  
Flight Mass Spectrometry ◽  
Photodiode Array Detector ◽  
Photodiode Array

Tibouchina pulchra (Cham.) Cogn. is a plant native to Brazil whose genus and family (Melastomataceae) are poorly studied with regards to its metabolite profile. Phenolic pigments of pink flowers were studied by ultra-performance liquid chromatography with a photodiode array detector and electrospray ionization quadrupole time-of-flight mass spectrometry. Therein, twenty-three flavonoids were identified with eight flavonols isolated by preparative high-performance liquid chromatography and analysed by one- and two-dimensional nuclear magnetic resonance. Kaempferol derivatives were the main flavonols, encompassing almost half of the detected compounds with different substitution patterns, such as glucoside, pentosides, galloyl-glucoside, p-coumaroyl-glucoside, and glucuronide. Concerning the anthocyanins, petunidin p-coumaroyl-hexoside acetylpentoside and malvidin p-coumaroyl-hexoside acetylpentoside were identified and agreed with previous reports on acylated anthocyanins from Melastomataceae. A new kaempferol glucoside was identified as kaempferol-(2′′-O-methyl)-4′-O-α-d-glucopyranoside. Moreover, twelve compounds were described for the first time in the genus with five being new to the family, contributing to the chemical characterisation of these taxa.

scholarly journals An Environmentally Friendly Practice Used in Olive Cultivation Capable of Increasing Commercial Interest in Waste Products from Oil Processing

Antioxidants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 466
Author(s):  
Irene Dini ◽  
Giulia Graziani ◽  
Francalisa Luisa Fedele ◽  
Andrea Sicari ◽  
Francesco Vinale ◽  
...  
Keyword(s):  
Phenolic Compounds ◽  
Bioactive Compounds ◽  
High Performance ◽  
Mevalonate Pathway ◽  
Total Concentration ◽  
Economic Value ◽  
Antioxidant Potential ◽  
Total Phenolic ◽  
Waste Products ◽  
Vegetation Water

In the Rural Development Plan (2014–2020), the European Commission encouraged the conversion and supported the maintenance of organic farming. Organic olive oil (bioEVOO) production involves the use of environmentally sustainable fertilizers and the recycling of olive pomace (Pom) and olive vegetation waters (VW) to reduce the environmental impact of these wastes. An ecofriendly way to recycle olive wastes is to reuse them to extract bioactive compounds. In this study, the total phenolic compounds content, their profile and dosage, the antioxidant action in oil, pomace, and vegetation water was evaluated when the Trichoderma harzianum M10 was used as a biostimulant in agriculture. Two spectrophotometric tests (2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azinobis (3-ethylbenzothiazoline-6-sulfonic) acid (ABTS)) evaluated the antioxidant potential of samples, a spectrophotometric method estimated total phenolic content, and an Ultra-High-Performance Liquid Chromatography (UHPLC)–Orbitrap method evaluated the phenolics profile. Our results showed that the biostimulation improved the antioxidant potential and the total concentration of phenolics in the bioEVOO and bio-pomace (bioPom) samples and mainly enhanced, among all classes of phenolic compounds, the production of the flavonoids and the secoiridoids. Moreover, they demonstrated the Trichoderma action in the mevalonate pathway to produce phenols for the first time. The decisive action of the Thricoderma on the production of phenolic compounds increases the economic value of the waste materials as a source of bioactive compounds useful for the pharmaceutical, cosmetic, and food industries.

scholarly journals Biotransformation of soy whey into a novel functional beverage by Cordyceps militaris SN-18

2021 ◽  
Vol 3 (1) ◽  
Author(s):  
Yiqiang Dai ◽  
Jianzhong Zhou ◽  
Lixia Wang ◽  
Mingsheng Dong ◽  
Xiudong Xia
Keyword(s):  
Dna Damage ◽  
High Performance ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Essential Amino Acids ◽  
Cordyceps Militaris ◽  
Total Phenolic ◽  
Functional Beverages ◽  
Radical Scavenging Ability ◽  
Phenolic And Flavonoid

Abstract Soy whey, a liquid nutritional by-product of soybean manufacture, is rich in proteins, oligosaccharides and isoflavones. Soy whey can be used to produce functional beverages, instead of discarding it as a waste. In this study, unfermented soy whey (USW) and Cordyceps militaris SN-18-fermented soy whey (FSW) were investigated and compared for their physicochemical and functional properties by high performance liquid chromatography (HPLC) and DNA damage assay. Results show that C. militaris SN-18 fermentation could increase the contents of essential amino acids, total phenolic and flavonoid and isoflavone aglycones and eliminate the oligosaccharides in soy whey. Furthermore, C. militaris SN-18 could significantly enhance the ABTS radical scavenging ability, reducing power and ferric reducing power of soy whey, and its fermented products could prominently attenuate Fenton reaction-induced DNA damage. These findings indicate that soy whey can potentially be converted into a novel soy functional beverage by C. militaris SN-18 fermentation. Graphical abstract

scholarly journals Solvent Mixture Optimization in the Extraction of Bioactive Compounds and Antioxidant Activities from Garlic (Allium sativum L.)

Molecules ◽  
2021 ◽  
Vol 26 (19) ◽  
pp. 6026
Author(s):  
Vytória Piscitelli Cavalcanti ◽  
Smail Aazza ◽  
Suzan Kelly Vilela Bertolucci ◽  
João Pedro Miranda Rocha ◽  
Adriane Duarte Coelho ◽  
...  
Keyword(s):  
Binary Mixture ◽  
Bioactive Compounds ◽  
Antioxidant Activities ◽  
Pure Water ◽  
Extraction Procedure ◽  
Solvent Mixture ◽  
Total Phenolic ◽  
Solvent Mixtures ◽  
Garlic Powder ◽  
Dpph Scavenging

Garlic is a health promoter that has important bioactive compounds. The bioactive extraction is an important step in the analysis of constituents present in plant preparations. The purpose of this study is to optimize the extraction with the best proportion of solvents to obtain total phenolic compounds (TPC) and thiosulfinates (TS) from dried garlic powder, and evaluate the antioxidant activities of the optimized extracts. A statistical mixture simplex axial design was used to evaluate the effect of solvents (water, ethanol, and acetone), as well as mixtures of these solvents, after two ultrasound extraction cycles of 15 min. Results showed that solvent mixtures with a high portion of water and pure water were efficient for TPC and TS recovery through this extraction procedure. According to the regression model computed, the most significant solvent mixtures to obtain high TPC and TS recovery from dried garlic powder are, respectively, the binary mixture with 75% water and 25% acetone and pure water. These optimized extracts presented oxygen radical absorbance capacity. Pure water was better for total antioxidant capacity, and the binary mixture of water–acetone (75:25) was better for DPPH scavenging activity. These optimized extracts can be used for industrial and research applications.

scholarly journals Optimization of Extraction Conditions of Bioactive Compounds by Ultrasonic-Assisted Extraction from Artichoke Wastes

2021 ◽  
Vol 68 (3) ◽  
pp. 658-666
Author(s):  
Izzet Turker ◽  
Hilal Isleroglu
Keyword(s):  
Antioxidant Capacity ◽  
Bioactive Compounds ◽  
Extraction Process ◽  
Extraction Yield ◽  
Extraction Time ◽  
Total Phenolic ◽  
Assisted Extraction ◽  
Ultrasonic Assisted ◽  
Extraction Processes ◽  
Ultrasonic Assisted Extraction

In this study, bioactive compounds were extracted by ultrasonic-assisted extraction and classical extraction processes using distilled water as solvent from artichoke leaves which are considered as agricultural wastes. Antioxidant capacity, total phenolic and total flavonoid content values of the obtained bioactive extracts were determined, and extraction yields and times were evaluated to compare the extraction processes. Also, the optimum extraction conditions of ultrasonic-assisted extraction (extraction time and ultrasonic power) which provide the highest extraction yield were determined using D-optimal design by ‘desirability’ function approach. According to the results, bioactive extracts having high antioxidant capacity were obtained at shorter times and higher extraction yields were achieved by ultrasonic-assisted extraction process than classical extraction. The highest extraction yield was estimated as 98.46% with an application of 20.05 minutes of extraction time and 65.02% of ultrasonic amplitude for the ultrasonic-assisted extraction process.

scholarly journals Clonal Variation in the Bark Chemical Properties of Hybrid Aspen: Potential for Added Value Chemicals

Molecules ◽  
2020 ◽  
Vol 25 (19) ◽  
pp. 4403
Author(s):  
Pasi Korkalo ◽  
Risto Korpinen ◽  
Egbert Beuker ◽  
Tytti Sarjala ◽  
Jarkko Hellström ◽  
...  
Keyword(s):  
Chemical Properties ◽  
Hot Water ◽  
Raw Material ◽  
Added Value ◽  
Clonal Variation ◽  
Chemical Components ◽  
Total Phenolic ◽  
Hybrid Aspen ◽  
Alternative Source ◽  
Water Extracts

This study aims to promote comprehensive utilization of woody biomass by providing a knowledgebase on the utility of aspen bark as a new alternative source for fossil-based chemicals. The research focused on the analysis of clonal variation in: (1) major chemical components, i.e., hemicelluloses, cellulose, and lignin; (2) extraneous materials, i.e., bark extractives, and suberic acid; (3) condensed tannins content and composition; and (4) screening differences in antioxidative properties and total phenolic content of hot water extracts and ethanol-water extracts of hybrid aspen bark. Results of this study, the discovery of clonal variation in utilizable chemicals, pave the way for further research on added-value potential of under-utilized hybrid aspen and its bark. Clonal variation was found in notable part of chemicals with potential for utilization. Based on the results, an appropriate bark raw material can be selected for tailored processing, thus improving the resource efficiency. The results also indicate that by applying cascade processing concepts, bark chemical substances could be more efficiently utilized with more environmentally friendly methods.

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