scholarly journals Surface Modification of Porous Titanium Discs Using Femtosecond Laser Structuring

Metals ◽  
2020 ◽  
Vol 10 (6) ◽  
pp. 748 ◽  
Author(s):  
Ángel Rodríguez ◽  
Paloma Trueba ◽  
José Manuel Amado ◽  
María José Tobar ◽  
Mercè Giner ◽  
...  
Keyword(s):  
Femtosecond Laser ◽  
Bone Ingrowth ◽  
Hierarchical Structures ◽  
Young Modulus ◽  
Porous Titanium ◽  
Titanium Implants ◽  
Confocal Laser ◽  
In Vitro Test ◽  
Implant Surface

The failure of titanium implants is associated with two main problems that include the bone resorption and fracture of the surrounding bone tissue (stiffness incompatibility) and implant loosening (poor osseointegration). The development of porous titanium implants with low Young modulus solve the stress shielding phenomenon, while the modification of the implant surface must be implemented to promote a fast bond between the implant and bone. In this work, femtosecond laser micromachining was applied to modify the topography of the surface of Ti porous samples obtained by a space-holder technique to obtain hierarchical structures (micro and nano roughness patterns) to enhance osseointegration. Scanning electron microscopy, confocal laser microscopy, and image analysis were used for characterization of the surface morphology, roughness, and porosity before and after performing the laser treatment. Based on these results, the effect of the treatment on the mechanical behavior of the samples was estimated. In addition, a preliminary in-vitro test was performed to verify the adhesion of osteoblasts (filopodia presence) on modified titanium surface. Results revealed that laser texturing generated clusters of micro-holes and micro-columns both on the flat surface of the samples and inside the macro-pores, and periodic nanometric structures across the entire surface. The porous substrate offers suitable biomechanics (stiffness and yield strength) and bio-functional behavior (bone ingrowth and osseointegration), which improves the clinic success of titanium implants.

scholarly journals Macrophagic Inflammatory Response Next to Dental Implants with Different Macro- and Micro-Structure: An In Vitro Study

2021 ◽  
Vol 11 (12) ◽  
pp. 5324
Author(s):  
Maria Menini ◽  
Francesca Delucchi ◽  
Domenico Baldi ◽  
Francesco Pera ◽  
Francesco Bagnasco ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Dental Implants ◽  
In Vitro Study ◽  
Titanium Implants ◽  
Implant Surface ◽  
Bone Implant ◽  
Optimal Outcomes ◽  
Negative Controls ◽  
Inflammatory Effect

(1) Background: Intrinsic characteristics of the implant surface and the possible presence of endotoxins may affect the bone–implant interface and cause an inflammatory response. This study aims to evaluate the possible inflammatory response induced in vitro in macrophages in contact with five different commercially available dental implants. (2) Methods: one zirconia implant NobelPearl® (Nobel Biocare) and four titanium implants, Syra® (Sweden & Martina), Prama® (Sweden & Martina), 3iT3® (Biomet 3i) and Shard® (Mech & Human), were evaluated. After 4 h of contact of murine macrophage cells J774a.1 with the implants, the total RNA was extracted, transcribed to cDNA and the gene expression of the macrophages was evaluated by quantitative PCR (qPCR) in relation to the following genes: GAPDH, YWHAZ, IL1β, IL6, TNFα, NOS2, MMP-9, MMP-8 and TIMP3. The results were statistically analyzed and compared with negative controls. (3) Results: No implant triggered a significant inflammatory response in macrophages, although 3iT3 exhibited a slight pro-inflammatory effect compared to other samples. (4) Conclusions: All the samples showed optimal outcomes without any inflammatory stimulus on the examined macrophagic cells.

scholarly journals Electrical Impedance of Surface Modified Porous Titanium Implants with Femtosecond Laser

Materials ◽  
2022 ◽  
Vol 15 (2) ◽  
pp. 461
Author(s):  
Paula Navarro ◽  
Alberto Olmo ◽  
Mercè Giner ◽  
Marleny Rodríguez-Albelo ◽  
Ángel Rodríguez ◽  
...  
Keyword(s):  
Cell Culture ◽  
Femtosecond Laser ◽  
Laser Treatment ◽  
Electrical Impedance ◽  
Porous Titanium ◽  
Titanium Implants ◽  
Electrical Impedance Spectroscopy ◽  
Wide Range ◽  
Titanium Surfaces ◽  
Surface Modified

The chemical composition and surface topography of titanium implants are essential to improve implant osseointegration. The present work studies a non-invasive alternative of electrical impedance spectroscopy for the characterization of the macroporosity inherent to the manufacturing process and the effect of the surface treatment with femtosecond laser of titanium discs. Osteoblasts cell culture growths on the titanium surfaces of the laser-treated discs were also studied with this method. The measurements obtained showed that the femtosecond laser treatment of the samples and cell culture produced a significant increase (around 50%) in the absolute value of the electrical impedance module, which could be characterized in a wide range of frequencies (being more relevant at 500 MHz). Results have revealed the potential of this measurement technique, in terms of advantages, in comparison to tiresome and expensive techniques, allowing semi-quantitatively relating impedance measurements to porosity content, as well as detecting the effect of surface modification, generated by laser treatment and cell culture.

scholarly journals Electrolytic cleaning of an implant surface in comparison to an air‐water‐powder spray – An in vitro test

2019 ◽  
Vol 30 (S19) ◽  
pp. 189-189
Author(s):  
Holger Zipprich ◽  
Markus Schlee ◽  
Urs Brodbeck ◽  
Christoph Ratka
Keyword(s):  
In Vitro Test ◽  
Implant Surface ◽  
Vitro Test

scholarly journals UV Light Assisted Coating Method of Polyphenol Caffeic Acid and Mediated Immobilization of Metallic Silver Particles for Antibacterial Implant Surface Modification

Polymers ◽  
2019 ◽  
Vol 11 (7) ◽  
pp. 1200 ◽  
Author(s):  
Ji Yeon Lee ◽  
Ludwig Erik Aguilar ◽  
Chan Hee Park ◽  
Cheol Sang Kim
Keyword(s):  
Caffeic Acid ◽  
Mechanical Stability ◽  
Uv Light ◽  
Oxidative Polymerization ◽  
Titanium Implants ◽  
Metallic Silver ◽  
Functional Coatings ◽  
Implant Surface ◽  
Coating Application

Titanium implants are extensively used in biomedical applications due to their excellent biocompatibility, corrosion resistance, and superb mechanical stability. In this work, we present the use of polycaffeic acid (PCA) to immobilize metallic silver on the surface of titanium materials to prevent implant bacterial infection. Caffeic acid is a plant-derived phenolic compound, rich in catechol moieties and it can form functional coatings using alkaline buffers and with UV irradiation. This combination can trigger oxidative polymerization and deposition on the surface of metallic substrates. Using PCA can also give advantages in bone implants in decreasing inflammation by decelerating macrophage and osteoclast activity. Here, chemical and physical properties were investigated using FE-SEM, EDS, XPS, AFM, and contact angle. The in vitro biocompatibility and antibacterial studies show that PCA with metallic silver can inhibit bacterial growth, and proliferation of MC-3T3 cells was observed. Therefore, our results suggest that the introduced approach can be considered as a potential method for functional implant coating application in the orthopedic field.

scholarly journals In Vitro Assessment of the Functional Dynamics of Titanium with Surface Coating of Hydroxyapatite Nanoparticles

Materials ◽  
2019 ◽  
Vol 12 (5) ◽  
pp. 840 ◽  
Author(s):  
José Henrique de Lima Cavalcanti ◽  
Patrícia Matos ◽  
Cresus Vinícius Depes de Gouvêa ◽  
Waldimir Carvalho ◽  
José Luis Calvo-Guirado ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Adhesion Formation ◽  
Titanium Implants ◽  
Tissue Response ◽  
Mesenchymal Progenitor Cells ◽  
Machined Surface ◽  
Implant Surface ◽  
Sem Images ◽  
Vitro Assay

Manipulation of implant surface characteristics constitutes a promising strategy for improving cell growth and tissue response on a variety of materials with different surface topographies. Mesenchymal progenitor cells with a capacity to respond to titanium surface stimuli and differentiate into osteoblasts were used to perform comparative tests between two different implant topographies, including their functional interaction with pre-osteoblasts directly seeded onto the implants. Functional analysis of nanostructured implant surfaces was performed by in vitro assay analysis. The machined surface of titanium implants (mach group) was used as a control and compared with a nanoparticle HA activated surface implant (nano group), developed by the deposition of pure crystalline hydroxyapatite. Cell culture on the nano group surface resulted in higher cell adhesion and cultured osteoblast viability compared with the mach group. Scanning electron microscope (SEM) images revealed a stable interaction, indicated by the presence of focal cell adhesion formation. These results together with positive mineralization assays showed the nano group to be an excellent scaffold for bone-implant integration.

Controlled implant/soft tissue interaction by nanoscale surface modifications of 3D porous titanium implants

Nanoscale ◽  
2015 ◽  
Vol 7 (21) ◽  
pp. 9908-9918 ◽  
Author(s):  
Elisabeth Rieger ◽  
Agnès Dupret-Bories ◽  
Laetitia Salou ◽  
Marie-Helene Metz-Boutigue ◽  
Pierre Layrolle ◽  
...  
Keyword(s):  
Surface Modification ◽  
Soft Tissue ◽  
Surface Modifications ◽  
Porous Titanium ◽  
Titanium Implants ◽  
Tissue Response ◽  
Soft Tissue Response ◽  
Modification Of Titanium

Nanoscale surface modification of titanium microbeads can control the soft tissue response in vitro and in vivo.

Novel Micro-CT Based 3-Dimentional Structural Analyses of Porous Biomaterials

2007 ◽  
Vol 330-332 ◽  
pp. 967-970 ◽  
Author(s):  
B. Otsuki ◽  
Mitsuru Takemoto ◽  
Shunsuke Fujibayashi ◽  
Masashi Neo ◽  
Tadashi Kokubo ◽  
...  
Keyword(s):  
Network Structure ◽  
Bone Ingrowth ◽  
Average Pore Size ◽  
Three Dimensional ◽  
Porous Titanium ◽  
Titanium Implants ◽  
Average Pore ◽  
Tissue Ingrowth ◽  
Porous Biomaterials

A porous structure comprises pores and pore throats with a complex three-dimensional network structure, and many investigators have described the relationship between average pore size and the amount of bone ingrowth. However, the influence of network structure or pore throats for tissue ingrowth has rarely been discussed. Bioactive porous titanium implants with 48% porosity were analyzed using specific algorithms for three-dimensional analysis of interconnectivity based on a micro focus X-ray computed tomography system. In vivo histological analysis was performed using the very same implants implanted into the femoral condyles of male rabbits for 6 weeks. This matching study revealed that more poorly differentiated pores tended to have narrow pore throats, especially in their shorter routes to the outside. Data obtained suggest that this sort of novel analysis is useful for evaluating bone and tissue ingrowth into porous biomaterials.

scholarly journals Impact of High-Altitude Hypoxia on Early Osseointegration With Bioactive Titanium

2021 ◽  
Vol 12 ◽  
Author(s):  
Yarong Wang ◽  
Zekun Gan ◽  
Haibin Lu ◽  
Ziyi Liu ◽  
Peng Shang ◽  
...  
Keyword(s):  
Bone Formation ◽  
High Altitude ◽  
Bone Repair ◽  
Titanium Implant ◽  
Titanium Implants ◽  
Implant Surface ◽  
Altitude Hypoxia ◽  
Oral Implants ◽  
Hypoxic Environment

Nowadays, the bone osseointegration in different environments is comparable, but the mechanism is unclear. This study aimed to investigate the osseointegration of different bioactive titanium surfaces under normoxic or high-altitude hypoxic environments. Titanium implants were subjected to one of two surface treatments: (1) sanding, blasting, and acid etching to obtain a rough surface, or (2) extensive polishing to obtain a smooth surface. Changes in the morphology, proliferation, and protein expression of osteoblasts on the rough and smooth surfaces were examined, and bone formation was studied through western blotting and animal-based experiments. Our findings found that a hypoxic environment and rough titanium implant surface promoted the osteogenic differentiation of osteoblasts and activated the JAK1/STAT1/HIF-1α pathway in vitro. The animal study revealed that following implant insertion in tibia of rabbit, bone repair at high altitudes was slower than that at low altitudes (i.e., in plains) after 2weeks; however, bone formation did not differ significantly after 4weeks. The results of our study showed that: (1) The altitude hypoxia environment would affect the early osseointegration of titanium implants while titanium implants with rough surfaces can mitigate the effects of this hypoxic environment on osseointegration, (2) the mechanism may be related to the activation of JAK1/STAT1/HIF-1α pathway, and (3) our results suggest the osteogenesis of titanium implants, such as oral implants, is closely related to the oxygen environment. Clinical doctors, especially dentists, should pay attention to the influence of hypoxia on early osseointegration in patients with high altitude. For example, it is better to choose an implant system with rough implant surface in the oral cavity of patients with tooth loss at high altitude.

Fabrication of bioactive 3D printed porous titanium implants with Sr ion-incorporated zeolite coatings for bone ingrowth

2018 ◽  
Vol 6 (20) ◽  
pp. 3254-3261 ◽  
Author(s):  
Shuang Wang ◽  
Ruiyan Li ◽  
Dongdong Li ◽  
Zhi-Yong Zhang ◽  
Guancong Liu ◽  
...  
Keyword(s):  
Bone Ingrowth ◽  
Porous Titanium ◽  
Titanium Implants ◽  
3D Printed ◽  
Strontium Ion ◽  
Zeolite Coatings

Strontium ion incorporated zeolites are uniformly fabricated on a 3D printed porous titanium scaffold for bone ingrowth.

Bioactivity and Surface Characteristics of Titanium Implants Following Various Surface Treatments: An In Vitro Study

2015 ◽  
Vol 41 (5) ◽  
pp. e183-e188 ◽  
Author(s):  
Aswini Kumar K ◽  
Vinaya Bhatt ◽  
Manilal Balakrishnan ◽  
Mohamed Hashem ◽  
Sajith Vellappally ◽  
...  
Keyword(s):  
Surface Treatment ◽  
Calcium Ions ◽  
In Vitro Study ◽  
Surface Treatments ◽  
Titanium Implants ◽  
Vitro Study ◽  
Acid Etching ◽  
Implant Surface ◽  
Group A

This study compared the surface topography, hydrophilicity, and bioactivity of titanium implants after 3 different surface treatments (sandblasting and acid etching, modified sandblasting and acid etching, and thermal oxidation) with those of machined implants. One hundred indigenously manufactured threaded titanium implants were subjected to 3 methods of surface treatment. The surface roughness of the nontreated (Group A) and treated samples (Groups B through D) was evaluated with a scanning electron microscope (SEM) and profilometer. The wettability was visually examined using a colored dye solution. The calcium ions attached to the implant surface after immersing in simulated body fluid (SBF) were assessed on days 1, 2, and 7 with an atomic electron spectroscope. The data were analyzed statistically. The SBF test allowed the precipitation of a calcium phosphate layer on all surface-treated samples, as evidenced in the SEM analysis. A significantly higher amount of calcium ions and increased wettability were achieved in the thermally oxidized samples. The mean roughness was significantly lower in Group A (0.85 ± 0.07) compared to Group B (1.35 ± 0.17), Group C (1.40 ± 0.14), and Group D (1.36 ± 0.18). The observations from this in vitro study indicated that surface treatment of titanium improved the bioactivity. Moreover, results identified the implants that were sandblasted, acid etched, and then oxidized attracted more calcium ions.

Sign in / Sign up

Export Citation Format

Share Document