scholarly journals Convenient Agarose Preparation with Hydrogen Peroxide and Desulfation Process Analysis

Marine Drugs ◽  
2021 ◽  
Vol 19 (6) ◽  
pp. 297
Author(s):  
Cong Zhang ◽  
Ding An ◽  
Qiong Xiao ◽  
Fu-Quan Chen ◽  
Yong-Hui Zhang ◽  
...  
Keyword(s):  
Hydrogen Peroxide ◽  
Gel Electrophoresis ◽  
Process Analysis ◽  
Gel Strength ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Metal Sulfate ◽  
Sulfate Content ◽  
Sulfate Ions ◽  
Electrical Neutrality

Agarose is a natural seaweed polysaccharide and widely used in the medicine, food, and biological fields because of its high gel strength, non-toxicity, and electrical neutrality. The sulfate group is one of the main charged groups that affect the performance of agarose. In the present study, a simple, eco-friendly, and efficient method was explored for agarose preparation. After desulfation with hydrogen peroxide (H2O2), the sulfate content of agar reached 0.21%. Together with gel strength, electroendosmosis, gelling and melting temperature, the indicators of desulfated agar met the standards of commercially available agarose. Notably, the desulfated agar can be used as an agarose gel electrophoresis medium to separate DNA molecules, and the separation effect is as good as that of commercially available agarose. Further, the H2O2 desulfation process was analyzed. The addition of a hydroxyl radical (HO•) scavenger remarkably decreased the H2O2 desulfation rate, indicating that HO• has a certain role in agar desulfation. Sulfate content detection indicated that sulfur was removed from agar molecules in the form of sulfate ions (SO42−) and metal sulfate. The band absence at 850 cm−1 indicated that the sulfate groups at C-4 of D-galactose in sulfated galactan were eliminated.

scholarly journals Decreased Sulfate Content and Zeta Potential Distinguish Glycosaminoglycans of the Extracellular Matrix of Osteoarthritis Cartilage

2021 ◽  
Vol 8 ◽  
Author(s):  
Rodolfo de Melo Nunes ◽  
Virgínia Claudia Carneiro Girão ◽  
Pablyana Leila Rodrigues Cunha ◽  
Judith Pessoa Andrade Feitosa ◽  
Ana Carolina Matias Dinelly Pinto ◽  
...  
Keyword(s):  
Zeta Potential ◽  
Gel Electrophoresis ◽  
Joint Arthroplasty ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Osteoarthritis Cartilage ◽  
Sulfate Content ◽  
Normal Cartilage ◽  
Men And Women ◽  
Fracture Control

We aimed to determine the characteristics that distinguish glycosaminoglycans (GAGs) from osteoarthritis (OA) and normal cartilage and from men and women. Cartilage samples from 30 patients subjected to total joint arthroplasty secondary to OA or fracture (control) were evaluated, and the GAG content (μg/mg dry cartilage) after proteolysis was determined by densitometry, using agarose-gel electrophoresis. Relative percentages of carbon (C), nitrogen (N), and sulfur (S) in GAGs were determined by elemental microanalysis, as well as the zeta potential. Seventeen samples (56.6%) were from patients >70 years old, with 20 (66.6%) from women, and most [20 (66.6%)] were from the hip. The GAG content was similar regardless of patients being >/≤ 70 years old with 96.5 ± 63.5 and 78.5 ± 38.5 μg/mg (P = 0.1917), respectively. GAG content was higher in women as compared to men, with 89.5 ± 34.3 and 51.8 ± 13.3 μg/mg, respectively (P = 0.0022), as well as in OA than fracture samples, with 98.4 ± 63.5 and 63.6 ± 19.6 μg/mg, respectively (P = 0.0355). The GAG extracted from the cartilage of patients >70 years old had increase in N, and there were no gender differences regarding GAG elemental analysis. GAG from OA had a highly significant (P = 0.0005) decrease in S% (1.79% ± 0.25%), as compared to fracture samples (2.3% ± 0.19%), with an associated and significant (P = 0.0001) reduction of the zeta potential in the OA group. This is the first report of a reduced S content in GAG from OA patients, which is associated with a reduced zeta potential.

scholarly journals Chemical and photo-induced nuclease activity of a novel minor groove DNA binder Cu(II) complex

2019 ◽  
Vol 84 (6) ◽  
pp. 563-574
Author(s):  
Ufuk Yildiz ◽  
Burak Coban
Keyword(s):  
Hydrogen Peroxide ◽  
Metal Complex ◽  
Dna Cleavage ◽  
Gel Electrophoresis ◽  
Nuclease Activity ◽  
Dna Interaction ◽  
Minor Groove ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
New Type

A new type of copper(II) metal complex containing 1,10-phenanthroline (phen) and 8-(difluoromethoxy)-3,4-dihydro-2H-[1,3]thiazino[3,2-a]benzimidazole (dtb) ligands was prepared and characterized. The ds-DNA interaction of the complex was studied by UV?Vis spectrophotometry, competitive fluorometric titration with ethidium bromide (EB) and 4?,6-diamidino-2-phenylindole (DAPI), viscosity measurements and agarose gel electrophoresis. The results show that the complex can bind to ds-DNA in the minor groove by displacing DAPI molecules. DNA cleavage mechanism studies revealed that hydrogen peroxide radicals are responsible for DNA oxidative cleavage.

scholarly journals Effect of a 50-Hz Magnetic Field on the Degradation of Plasmid in the Presence of Cu(II) Ions and Hydrogen Peroxide

2017 ◽  
Vol 9 (4) ◽  
pp. 40 ◽  
Author(s):  
Yuuichi Kawashima ◽  
Honoka Fujii ◽  
Maresuke Nakayama ◽  
Masahiko Hasegawa ◽  
Satoru Shimanuki ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Hydrogen Peroxide ◽  
Gel Electrophoresis ◽  
Dna Strand Breaks ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Strand Breaks ◽  
Line Frequency ◽  
Dna Strand ◽  
The Magnetic Field

Power-line frequency (50/60 Hz) magnetic fields enhance DNA strand breaks in the cell. In order to understand the molecular mechanism underlying this phenomenon, we analyzed the conversion of plasmid from the supercoiled to the linear form. This conversion is promoted by hydroxyl radical generated by the reaction between Cu2+ and H2O2. The plasmid pHSG298 was incubated with 1 mM Cu2+ and 1 mM H2O2 and exposed to a 1.2 mT, 50-Hz magnetic field for 30, 60, and 90 min. The conversion of supercoiled DNA to linear DNA was analyzed by agarose gel electrophoresis. We found that exposure to the magnetic field for 90 min significantly enhanced the degree of conversion.

Luminography – an Alternative Assay for Detection of von Willebrand Factor Multimers

1988 ◽  
Vol 60 (02) ◽  
pp. 133-136 ◽  
Author(s):  
R Schneppenheim ◽  
H Plendl ◽  
U Budde
Keyword(s):  
Von Willebrand Factor ◽  
Gel Electrophoresis ◽  
Detection Methods ◽  
Agarose Gel ◽  
Agarose Gel Electrophoresis ◽  
Luminescence Assay ◽  
Von Willebrand ◽  
Willebrand Factor

SummaryA luminescence assay was adapted for detection of von Willebrand factor multimers subsequent to SDS-agarose gel electrophoresis and electroblotting onto nitrocellulose. The method is as fast as chromogenic detection methods and appears to be as sensitive as autoradiography without the disadvantages of the latter.

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