scholarly journals Modulation of the Liver Protein Carbonylome by the Combined Effect of Marine Omega-3 PUFAs and Grape Polyphenols Supplementation in Rats Fed an Obesogenic High Fat and High Sucrose Diet

Marine Drugs ◽  
2019 ◽  
Vol 18 (1) ◽  
pp. 34 ◽  
Author(s):  
Lucía Méndez ◽  
Silvia Muñoz ◽  
Bernat Miralles-Pérez ◽  
Maria Rosa Nogués ◽  
Sara Ramos-Romero ◽  
...  
Keyword(s):  
Metabolic Disorders ◽  
Protein Identification ◽  
Grape Seed ◽  
Protein Carbonyls ◽  
Individual Response ◽  
Liver Protein ◽  
Omega 3 ◽  
High Fat ◽  
Grape Seed Polyphenols ◽  
High Sucrose

Diet-induced obesity has been linked to metabolic disorders such as cardiovascular diseases and type 2 diabetes. A factor linking diet to metabolic disorders is oxidative stress, which can damage biomolecules, especially proteins. The present study was designed to investigate the effect of marine omega-3 polyunsaturated fatty acids (PUFAs) (eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)) and their combination with grape seed polyphenols (GSE) on carbonyl-modified proteins from plasma and liver in Wistar Kyoto rats fed an obesogenic diet, namely high-fat and high-sucrose (HFHS) diet. A proteomics approach consisting of fluorescein 5-thiosemicarbazide (FTSC) labelling of protein carbonyls, visualization of FTSC-labelled protein on 1-DE or 2-DE gels, and protein identification by MS/MS was used for the protein oxidation assessment. Results showed the efficiency of the combination of both bioactive compounds in decreasing the total protein carbonylation induced by HFHS diet in both plasma and liver. The analysis of carbonylated protein targets, also referred to as the ‘carbonylome’, revealed an individual response of liver proteins to supplements and a modulatory effect on specific metabolic pathways and processes due to, at least in part, the control exerted by the supplements on the liver protein carbonylome. This investigation highlights the additive effect of dietary fish oils and grape seed polyphenols in modulating in vivo oxidative damage of proteins induced by the consumption of HFHS diets.

scholarly journals FFAR4 (GPR120) Signaling Is Not Required for Anti-Inflammatory and Insulin-Sensitizing Effects of Omega-3 Fatty Acids

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Simone Isling Pærregaard ◽  
Marianne Agerholm ◽  
Annette Karen Serup ◽  
Tao Ma ◽  
Bente Kiens ◽  
...  
Keyword(s):  
Insulin Resistance ◽  
Fatty Acids ◽  
Whole Body ◽  
Low Grade ◽  
Omega 3 ◽  
High Fat ◽  
Beneficial Effects ◽  
Protein Levels ◽  
Anti Inflammatory ◽  
High Sucrose

Free fatty acid receptor-4 (FFAR4), also known as GPR120, has been reported to mediate the beneficial effects of omega-3 polyunsaturated fatty acids (ω3-PUFAs) by inducing an anti-inflammatory immune response. Thus, activation of FFAR4 has been reported to ameliorate chronic low-grade inflammation and insulin resistance accompanying obesity. However, conflicting reports on the role of FFAR4 in mediating the effects ofω3-PUFAs are emerging, suggesting that FFAR4 may not be the sole effector. Hence analyses of the importance of this receptor in relation to other signaling pathways and prominent effects ofω3-PUFAs remain to be elucidated. In the present study, we usedFfar4knockouts (KO) and heterozygous (HET) mice fed either low fat, low sucrose reference diet; high fat, high sucroseω3-PUFA; or high fat, high sucroseω6-PUFA diet for 36 weeks. We demonstrate that both KO and HET mice fedω3-PUFAs were protected against obesity, hepatic triacylglycerol accumulation, and whole-body insulin resistance. Moreover,ω3-PUFA fed mice had increased circulating protein levels of the anti-inflammatory adipokine, adiponectin, decreased fasting insulin levels, and decreased mRNA expression of several proinflammatory molecules within visceral adipose tissue. In conclusion, we find that FFAR4 signaling is not required for the reported anti-inflammatory and insulin-sensitizing effects mediated byω3-PUFAs.

High fat high sucrose diet-induced dyslipidemia in guinea pigs

2021 ◽  
Vol 130 (4) ◽  
pp. 1226-1234
Author(s):  
Cynthia R. Muller ◽  
Alexander T. Williams ◽  
Allyn M. Eaker ◽  
Fernando Dos Santos ◽  
Andre F. Palmer ◽  
...  
Keyword(s):  
Animal Model ◽  
Liver Disease ◽  
Guinea Pigs ◽  
Metabolic Disorders ◽  
Cardiovascular Risks ◽  
High Fat ◽  
Sucrose Diet ◽  
Strong Relation ◽  
High Sucrose Diet ◽  
High Sucrose

In this study, we show a new animal model for diet-induced disease metabolic disorders without obesity in guinea pigs. Moreover, results suggest a strong relation between liver disease and increased cardiovascular risks.

Bifidobacteria attenuate the development of metabolic disorders, with inter- and intra-species differences

2018 ◽  
Vol 9 (6) ◽  
pp. 3509-3522 ◽  
Author(s):  
Guangsu Zhu ◽  
Fangli Ma ◽  
Gang Wang ◽  
Yuanyuan Wang ◽  
Jianxin Zhao ◽  
...  
Keyword(s):  
Metabolic Syndrome ◽  
Gut Microbiota ◽  
Metabolic Disorders ◽  
Species Differences ◽  
Microbiota Composition ◽  
High Fat ◽  
High Sucrose ◽  
Gut Microbiota Composition

Supplementation with specific Bifidobacterium strains will attenuate some hallmark parameters of metabolic syndrome in high-fat–high-sucrose treated rats, elevate the levels of SCFAs in gut and change the gut microbiota composition beneficially.

2038-P: Rice Endosperm Protein Intake in Childhood Suppresses the Development of High-Fat Diet-Induced Metabolic Disorders and Kidney Disease during Adulthood in Mice

Diabetes ◽  
2019 ◽  
Vol 68 (Supplement 1) ◽  
pp. 2038-P
Author(s):  
YUKI HIGUCHI ◽  
MICHIHIRO HOSOJIMA ◽  
HIDEYUKI KABASAWA ◽  
SHOJI KUWAHARA ◽  
RYOHEI KASEDA ◽  
...  
Keyword(s):  
Kidney Disease ◽  
High Fat Diet ◽  
Protein Intake ◽  
Metabolic Disorders ◽  
High Fat ◽  
Rice Endosperm ◽  
Endosperm Protein

scholarly journals Protective effects of a unique combination of nutritionally active ingredients on risk factors and gene expression associated with atherosclerosis in C57BL/6J mice fed a high fat diet

2021 ◽  
Author(s):  
Joe W. E. Moss ◽  
Jessica O Williams ◽  
Wijdan Al-Ahmadi ◽  
Victoria O'Morain ◽  
Yee-Hung Chan ◽  
...  
Keyword(s):  
Gene Expression ◽  
Risk Factors ◽  
Cardiovascular Disease ◽  
High Fat Diet ◽  
Inflammatory Disorder ◽  
Protective Effects ◽  
Unique Combination ◽  
Omega 3 ◽  
High Fat ◽  
Food Ingredients

Atherosclerosis, an inflammatory disorder of the vasculature and the underlying cause of cardiovascular disease, is responsible for one in three global deaths. Consumption of active food ingredients such as omega-3...

scholarly journals Dose-Responses Relationship in Glucose Lowering and Gut Dysbiosis to Saskatoon Berry Powder Supplementation in High Fat-High Sucrose Diet-Induced Insulin Resistant Mice

2021 ◽  
Vol 9 (8) ◽  
pp. 1553
Author(s):  
Ruozhi Zhao ◽  
Fei Huang ◽  
Garry X. Shen
Keyword(s):  
Inflammatory Markers ◽  
Plasminogen Activator Inhibitor ◽  
Model Assessment ◽  
High Fat ◽  
Plasminogen Activator Inhibitor 1 ◽  
Insulin Resistant ◽  
Gut Dysbiosis ◽  
Freeze Dried ◽  
Factor Α ◽  
High Sucrose

Administration of freeze-dried powder of Saskatoon berry (SB), a popular fruit enriched with antioxidants, reduced glucose level, inflammatory markers and gut microbiota disorder in high fat-high sucrose (HFHS) diet-induced insulin resistant mice. The present study examined the dose-response relationship in metabolic, inflammatory and gut microbiotic variables to SB power (SBp) supplementation in HFHS diet-fed mice. Male C57 BL/6J mice were fed with HFHS diet supplemented with 0, 1%, 2.5% or 5% SBp for 11 weeks. HFHS diet significantly increased the levels of fast plasma glucose (FPG), cholesterol, triglycerides, insulin, homeostatic model assessment of insulin resistance (HOMA-IR), tumor necrosis factor-α, monocyte chemotactic protein-1 and plasminogen activator inhibitor-1, but decreased fecal Bacteroidetes phylum bacteria and Muribaculaceae family bacteria compared to low fat diet. SBp dose-dependently reduced metabolic and inflammatory variables and gut dysbiosis in mice compared with mice receiving HFHS diet alone. Significant attenuation of HFHS diet-induced biochemical disorders were detected in mice receiving ≥1% SBp. The abundances of Muribaculaceae family bacteria negatively correlated with body weights, FPG, lipids, insulin, HOMA-IR and inflammatory markers in the mice. The results suggest that SBp supplementation dose-dependently attenuated HFHS diet-induced metabolic and inflammatory disorders, which was associated with the amelioration of gut dysbiosis in the mice.

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