scholarly journals Collagen Extraction Optimization from the Skin of the Small-Spotted Catshark (S. canicula) by Response Surface Methodology

Marine Drugs ◽  
2019 ◽  
Vol 17 (1) ◽  
pp. 40 ◽  
Author(s):  
María Blanco ◽  
José Antonio Vázquez ◽  
Ricardo I. Pérez-Martín ◽  
Carmen G. Sotelo
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Extraction Procedure ◽  
Amino Acid Content ◽  
Combined Effect ◽  
Human Consumption ◽  
Concentration Time ◽  
Sds Page ◽  
Skin Collagen ◽  
Pre Treatment

The small-spotted catshark is one of the most abundant elasmobranchs in the Northeastern Atlantic Ocean. Although its landings are devoted for human consumption, in general this species has low commercial value with high discard rates, reaching 100% in some European fisheries. The reduction of post-harvest losses (discards and by-products) by promotion of a full use of fishing captures is one of the main goals of EU fishing policies. As marine collagens are increasingly used as alternatives to mammalian collagens for cosmetics, tissue engineering, etc., fish skins represent an excellent and abundant source for obtaining this biomolecule. The aim of this study was to analyze the influence of chemical treatment concentration, temperature and time on the extractability of skin collagen from this species. Two experimental designs, one for each of the main stages of the process, were performed by means of Response Surface Methodology (RSM). The combined effect of NaOH concentration, time and temperature on the amount of collagen recovered in the first stage of the collagen extraction procedure was studied. Then, skins treated under optimal NaOH conditions were subjected to a second experimental design, to study the combined effect of AcOH concentration, time and temperature on the collagen recovery by means of yield, amino acid content and SDS-PAGE characterization. Values of independent variables maximizing collagen recovery were 4 °C, 2 hours and 0.1 M NaOH (pre-treatment) and 25 °C, 34 hours and 1 M AcOH (collagen extraction).

Enzyme-assisted Extraction for Optimized Recovery of Phenolic Bioactives from Peganum hermala Leaves Using Response Surface Methodology

2018 ◽  
Vol 17 (4) ◽  
pp. 349-354
Author(s):  
Qadir Rahman ◽  
Anwar Farooq ◽  
Amjad Gilani Mazhar ◽  
Nadeem Yaqoob Muhammad ◽  
Ahmad Mukhtar
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Enzyme Concentration ◽  
Coumaric Acid ◽  
Assisted Extraction ◽  
Pre Treatment ◽  
Optimized Conditions ◽  
Enzyme Complexes ◽  
Hydrolysis Of ◽  
Central Composite

This study investigates the effect of enzyme formulations (Zympex-014, Kemzyme dry-plus and Natuzyme) on recovery of phenolics from Peganum hermala (harmal) leaves, under optimized conditions using response surface methodology. As compared to the other enzyme complexes, the yield (34 g/100g) obtained through Zympex-014-assisted extraction was higher under optimized conditions such as time (75 min), temperature (70°C), pH (6.5) and enzyme concentration (5 g/100 g) using central composite design (CCD). Effectiveness of Zympex-014 towards hydrolysis of P. hermala leaves cell wall was examined by analyzing the control and enzyme-treated leave residues using scanning electron microscope (SEM). GC/MS characterization authenticated the presence of quercetin (1.44), gallic acid (0.23), caffeic acid (0.04), cinnamic acid (0.05), m-coumaric acid (0.23) and p-coumaric acid (0.37 μg/g) as the potent phenolics in Zympex-014 based extract. It can be concluded from the findings of the current work that pre-treatment of P. hermala leaves with Zympex-014 significantly enhanced the recovery of phenolics that supports its potential uses in the nutra-pharamaceutical industry.

scholarly journals Optimization of Xylose Production from Sugarcane Trash by Microwave-Maleic Acid Hydrolysis

REAKTOR ◽  
2020 ◽  
Vol 20 (2) ◽  
pp. 81-88
Author(s):  
Euis Hermiati ◽  
Maulida Oktaviani ◽  
Riksfardini Annisa Ermawar ◽  
Raden Permana Budi Laksana ◽  
Lutfi Nia Kholida ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Acid Hydrolysis ◽  
Response Surface ◽  
Acid Concentration ◽  
Maleic Acid ◽  
Sugar Alcohol ◽  
Quadratic Regression ◽  
Microwave Assisted ◽  
Concentration Time ◽  
Xylose Production

Sugarcane trash contains significant amount of xylan that could be hydrolysed to xylose. The xylose could be further fermented to produce xylitol, a sugar alcohol that has low calories and does not cause carries of teeth. In this study we optimized the production of xylose from sugarcane trash by microwave-assisted maleic acid hydrolysis using response surface methodology (RSM). The factors optimized were acid concentration, time, and temperature. The xylose yield based on the weight of initial biomass was determined and it served as a response variable. Results show that acid concentration and interaction between time and temperature had significant effect on xylose yield. The quadratic regression model generated from the optimization was fit and can be used to predict the xylose yield after hydrolysis with various combinations of acid concentration, time, and temperature. The optimum condition for xylose production from sugarcane trash was using maleic acid of 1.52%, and heating at 176 °C for 6.8 min. At this condition the yield of xylose was 24.3% per initial biomass or 0.243 g/ g biomass.Keywords: maleic acid; microwave heating; response surface methodology; sugarcane trash, xylose

scholarly journals Response surface methodology for the optimization of sludge solubilization by ultrasonic pre-treatment

2018 ◽  
Vol 113 ◽  
pp. 012177
Author(s):  
Mingyue Zheng ◽  
Xiaohui Zhang ◽  
Peng Lu ◽  
Qiguang Cao ◽  
Yuan Yuan ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Pre Treatment

A Thermomechanical Preprocessing for Pectin Isolation from Orange Peel with Optimisation by Response Surface Methodology

2008 ◽  
Vol 4 (1) ◽  
Author(s):  
Sid-Ahmed Rezzoug ◽  
Zoulikha Maache-Rezzoug ◽  
Frédéric Sannier ◽  
Karim Allaf
Keyword(s):  
Response Surface Methodology ◽  
Moisture Content ◽  
Response Surface ◽  
Processing Time ◽  
Initial Moisture Content ◽  
Control Sample ◽  
Orange Peel ◽  
Response Surfaces ◽  
Acid Extraction ◽  
Pre Treatment

The instantaneous controlled pressure drop process (or D.I.C process: ``Détente Instantanée Contrôlée") was used as a pre-treatment prior to pectin acid extraction from orange peel. This process involves subjecting the orange peel for a short time to steam pressure varying from 100 to 700 kPa, followed by an instantaneous decompression to vacuum at 5 kPa. Effects of processing pressure, moisture content of peels before the thermomechanical treatment and processing time were examined with response surface methodology. The optimal conditions were determined and the response surfaces were plotted from the mathematical models. The Fisher test and p-value indicated that both processing pressure and moisture content of peels before the pre-treatment had a highly significant effect on the pectin yield. The quadratic effect of processing pressure as well as the interaction effects of the initial moisture content and processing time also had a significant effect on the response. Moreover, the kinetics of pectin extraction showed that after few minutes of hydrolysis, the yields of pectin were systematically higher than that of the control sample and this is important from industrial point of view because the hydrolysis of pectin is generally performed in 10-15 minutes.

Optimization of Antioxidant Extraction from Freeze-dried Pulp, Peel, and Seed of Burmese grape (Baccaurea ramiflora Lour.) by Response Surface Methodology

2021 ◽  
Author(s):  
Mohammad Afzal Hossain ◽  
Md. Sakib Hossain
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Incubation Time ◽  
Maximum Yield ◽  
Extraction Procedure ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Solvent Concentration ◽  
Antioxidant Power ◽  
Freeze Dried

Abstract This study aimed to attain the optimum condition necessary for extracting the maximum yield of antioxidants from the freeze-dried pulp, peel, and seed of Burmese grape using response surface methodology (RSM). Solvent (ethanol) concentration (%), temperature (°C), and time (min) were taken as independent variables by factorial screening for the extraction procedure. After extraction, the antioxidant activity of all samples was determined employing 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, total phenolic compounds (TPC), and ferric reducing antioxidant power (FRAP) assay. The experiment's optimum conditions were 80% solvent concentration, 69.01°C temperature, and 30 min for pulp. The optimum extraction conditions were found at 80°C for 29.39 min incubation time using 52.12% concentrated solvent for seed. For peel, the solvent concentration of 41.62% was found optimum when the temperature of 50°C and 30 min incubation time were used. The actual values of TPC, FRAP, and DPPH for freeze-dried pulp, peel and seed extracts were close to the predicted values, which confirms the models’ validity. The Analysis of Variance (ANOVA) showed that the models were significant for TPC, DPPH, and FRAP values of peel, pulp, and seed at different levels (p < 0.001 to p < 0.05). The composite desirability of pulp, seed, and peel were 0.94, 0.98, and 0.85, respectively, which suggest that the developed model could be effectively used for antioxidants’ extraction from freeze-dried pulp, peel, and seed of Burmese grape.

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