Optimization of an Extraction Procedure for the Quantification of Vitamin E in Tomato and Broccoli using Response Surface Methodology

2000 ◽  
Vol 13 (1) ◽  
pp. 45-57 ◽  
Author(s):  
Junsoo Lee ◽  
Lin Ye ◽  
William O. Landen ◽  
Ronaldo R. Eitenmiller
Keyword(s):  
Response Surface Methodology ◽  
Vitamin E ◽  
Response Surface ◽  
Extraction Procedure

scholarly journals Collagen Extraction Optimization from the Skin of the Small-Spotted Catshark (S. canicula) by Response Surface Methodology

Marine Drugs ◽  
2019 ◽  
Vol 17 (1) ◽  
pp. 40 ◽  
Author(s):  
María Blanco ◽  
José Antonio Vázquez ◽  
Ricardo I. Pérez-Martín ◽  
Carmen G. Sotelo
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Extraction Procedure ◽  
Amino Acid Content ◽  
Combined Effect ◽  
Human Consumption ◽  
Concentration Time ◽  
Sds Page ◽  
Skin Collagen ◽  
Pre Treatment

The small-spotted catshark is one of the most abundant elasmobranchs in the Northeastern Atlantic Ocean. Although its landings are devoted for human consumption, in general this species has low commercial value with high discard rates, reaching 100% in some European fisheries. The reduction of post-harvest losses (discards and by-products) by promotion of a full use of fishing captures is one of the main goals of EU fishing policies. As marine collagens are increasingly used as alternatives to mammalian collagens for cosmetics, tissue engineering, etc., fish skins represent an excellent and abundant source for obtaining this biomolecule. The aim of this study was to analyze the influence of chemical treatment concentration, temperature and time on the extractability of skin collagen from this species. Two experimental designs, one for each of the main stages of the process, were performed by means of Response Surface Methodology (RSM). The combined effect of NaOH concentration, time and temperature on the amount of collagen recovered in the first stage of the collagen extraction procedure was studied. Then, skins treated under optimal NaOH conditions were subjected to a second experimental design, to study the combined effect of AcOH concentration, time and temperature on the collagen recovery by means of yield, amino acid content and SDS-PAGE characterization. Values of independent variables maximizing collagen recovery were 4 °C, 2 hours and 0.1 M NaOH (pre-treatment) and 25 °C, 34 hours and 1 M AcOH (collagen extraction).

Optimization of Antioxidant Extraction from Freeze-dried Pulp, Peel, and Seed of Burmese grape (Baccaurea ramiflora Lour.) by Response Surface Methodology

2021 ◽  
Author(s):  
Mohammad Afzal Hossain ◽  
Md. Sakib Hossain
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Incubation Time ◽  
Maximum Yield ◽  
Extraction Procedure ◽  
Radical Scavenging ◽  
Total Phenolic ◽  
Solvent Concentration ◽  
Antioxidant Power ◽  
Freeze Dried

Abstract This study aimed to attain the optimum condition necessary for extracting the maximum yield of antioxidants from the freeze-dried pulp, peel, and seed of Burmese grape using response surface methodology (RSM). Solvent (ethanol) concentration (%), temperature (°C), and time (min) were taken as independent variables by factorial screening for the extraction procedure. After extraction, the antioxidant activity of all samples was determined employing 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, total phenolic compounds (TPC), and ferric reducing antioxidant power (FRAP) assay. The experiment's optimum conditions were 80% solvent concentration, 69.01°C temperature, and 30 min for pulp. The optimum extraction conditions were found at 80°C for 29.39 min incubation time using 52.12% concentrated solvent for seed. For peel, the solvent concentration of 41.62% was found optimum when the temperature of 50°C and 30 min incubation time were used. The actual values of TPC, FRAP, and DPPH for freeze-dried pulp, peel and seed extracts were close to the predicted values, which confirms the models’ validity. The Analysis of Variance (ANOVA) showed that the models were significant for TPC, DPPH, and FRAP values of peel, pulp, and seed at different levels (p < 0.001 to p < 0.05). The composite desirability of pulp, seed, and peel were 0.94, 0.98, and 0.85, respectively, which suggest that the developed model could be effectively used for antioxidants’ extraction from freeze-dried pulp, peel, and seed of Burmese grape.

Optimization of Protein Extraction from Fermented and Non Fermented Perah Seed by using Response Surface Methodology

2014 ◽  
Vol 68 (5) ◽  
Author(s):  
Hamimah Satirah Mazlan ◽  
, Ida Idayu Muhamad ◽  
Nor Diana Hassan ◽  
Nuraimi Azlan Hadi Tan
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Protein Extraction ◽  
Extraction Procedure ◽  
Surface Concentration ◽  
Protein Yield ◽  
Contour Plot ◽  
Box Behnken Design ◽  
Independent Variables ◽  
Degree Equation

The optimum protein extraction from fermented and non-fermented Perah seed (Elateriospermumtapos) was investigated using the response surface methodology (RSM). A box-behnken design with three independent variables which are NaOH concentration (6, 8 and 10%), extraction time (10, 20 and 30 minutes) and solvent/meal ratio (50:1, 100:1 and 150:1, v/w) was used to study the response of protein yield. A second-degree equation for independent and response variables was produced from simulation to obtain the contour plot graphs. The best protein extraction procedure from both fermented and non-fermented Perah seed was obtained at 5.5% of solvent extraction, 40:1 ratio of solvent/meal and at 32 minutes of reaction time. Surface concentration and ratio of solvent/meal were found to influence the protein yield from fermented seed; meanwhile only the solvent concentration influenced protein yield from non-fermented seed. The maximum protein yields for both fermented and non-fermented Perah seeds were 18.0 g/100g and 5.0 g/100g seed meal respectively.  

scholarly journals Optimization of Porphyran Extraction from Pyropia yezoensis by Response Surface Methodology and Its Lipid-Lowering Effects

Marine Drugs ◽  
2021 ◽  
Vol 19 (2) ◽  
pp. 53
Author(s):  
Dan He ◽  
Liping Yan ◽  
Yingxia Hu ◽  
Qifang Wu ◽  
Mingjiang Wu ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Hepg2 Cells ◽  
Fat Body ◽  
Maximum Yield ◽  
Extraction Procedure ◽  
Physicochemical Characteristics ◽  
Pyropia Yezoensis ◽  
Lipid Lowering ◽  
Molar Ratio

Macroalgae polysaccharides are phytochemicals that are beneficial to human health. In this study, response surface methodology was applied to optimize the extraction procedure of Pyropia yezoensis porphyran (PYP). The optimum extraction parameters were: 100 °C (temperature), 120 min (time), and 29.32 mL/g (liquid–solid ratio), and the maximum yield of PYP was 22.15 ± 0.55%. The physicochemical characteristics of PPYP, purified from PYP, were analyzed, along with its lipid-lowering effect, using HepG2 cells and Drosophila melanogaster larvae. PPYP was a β-type sulfated hetero-rhamno-galactan-pyranose with a molecular weight of 151.6 kDa and a rhamnose-to-galactose molar ratio of 1:5.3. The results demonstrated that PPYP significantly reduced the triglyceride content in palmitic acid (PA)-induced HepG2 cells and high-sucrose-fed D. melanogaster larvae by regulating the expression of lipid metabolism-related genes, reducing lipogenesis and increasing fatty acid β-oxidation. To summarize, PPYP can lower lipid levels in HepG2 cells and larval fat body (the functional homolog tissue of the human liver), suggesting that PPYP may be administered as a potential marine lipid-lowering drug.

Improving the Analysis of Anthocyanidins from Blueberries Using Response Surface Methodology

2016 ◽  
Vol 9 (1) ◽  
pp. 41-52
Author(s):  
Steven W. Lloyd ◽  
Casey C. Grimm ◽  
Karen L. Bett-Garber ◽  
John C. Beaulieu ◽  
Deborah L. Boykin
Keyword(s):  
Response Surface Methodology ◽  
Response Surface ◽  
Freeze Drying ◽  
Incubation Temperature ◽  
Extraction Procedure ◽  
Test Tube ◽  
Acid Type ◽  
Health Promoting ◽  
Peak Areas ◽  
Hydrolysis Of

Background: Recent interest in the health promoting potential of anthocyanins points to the need for robust and reliable analytical methods. It is essential to know that the health promoting chemicals are present in juices and other products processed from whole fruit. Many different methods have been published using a wide variety of conditions for the hydrolysis of anthocyanins to anthocyanidins. Objective: To investigate the factors influencing the hydrolytic conversion efficiency. The optimum set of conditions will maximize the recovery of anthocyanidins. Method: Extraction procedure (freeze drying vs. direct liquid extraction), heating method (reflux vs. sealed vial), nitrogen purging and acid type were investigated. Response surface methodology was then used to find the optimum combination of incubation time, acid concentration and incubation temperature. Results: Anthocyanidin recovery can be maximized using this procedure: Freeze-dry homogenized fruit and extract with methanol:water:TFA, place 1 mL extract or juice in a test tube and add 440 µL 37% HCl, purge the tube with N2, seal with a PTFE lined cap, vortex, then heat at 99°C for 6.4 minutes. Filter the hydrolysate into an autosampler vial and analyze by UPLC immediately. Conclusion: Maximizing the recovery of anthocyanidins (by manipulating conditions in order to maximize peak areas) leads to a more accurate measure of the anthocyanidins present in blueberries.

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