scholarly journals Assessment of the Impact of Decellularization Methods on Mechanical Properties of Biocomposites Used as Skin Substitute

Materials ◽  
2021 ◽  
Vol 14 (17) ◽  
pp. 4785
Author(s):  
Bożena Gzik-Zroska ◽  
Kamil Joszko ◽  
Wojciech Wolański ◽  
Sławomir Suchoń ◽  
Michał Burkacki ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Statistical Analysis ◽  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
Young Modulus ◽  
Skin Substitute ◽  
Skin Substitutes ◽  
Sterilization Process ◽  
The Impact ◽  
Sterilization Effect

This work aimed to assess the impact of acellularization and sterilization methods on the mechanical properties of biocomposites used as a skin substitute. On the basis of the statistical analysis, it was ascertained that the values of the Young modulus for the samples before the sterilization process—only in the cases of substances such as: trypsin, 15% glycerol and dispase—changed in a statistically significant way. In the case of dispase, the Young modulus value before the sterilization process amounted to 66.6 MPa, for trypsin this value equalled 33.9 MPa, whereas for 15% glycerol it was 11 MPa. In the case of samples after the completion of the sterilization process, the analysis did not show any statistically significant differences between the obtained results of Young’s modulus depending on the respective reagents applied. It was confirmed that different methods of acellularization and the process of sterilization effect the alteration of mechanical properties of allogeneic skins. In the case of the decellularization method using SDS (Sodium Dodecyl Sulfate), liquid nitrogen and 85% glycerol the highest values of strain were observed. In the authors’ opinion, it is the above-mentioned methods that should be recommended in the process of preparation of skin substitutes.

scholarly journals The impact of molded pulp product process on the mechanical properties of molded Bleached Chemi-Thermo-Mechanical Pulp

2021 ◽  
Vol 2 (1) ◽  
Author(s):  
Claire Dislaire ◽  
Yves Grohens ◽  
Bastien Seantier ◽  
Marion Muzy
Keyword(s):  
Mechanical Properties ◽  
Water Uptake ◽  
Process Parameters ◽  
Manufacturing Process ◽  
Water Immersion ◽  
Young Modulus ◽  
Drying Time ◽  
Mechanical Pulp ◽  
Hygroscopic Properties ◽  
The Impact

AbstractThis study was carried out using bleached softwood Chemi-Thermo-Mechanical Pulp to evaluate the influence of Molded Pulp Products’ manufacturing process parameters on the finished products’ mechanical and hygroscopic properties. A Taguchi table was done to make 8 tests with specific process parameters such as moulds temperature, pulping time, drying time, and pressing time. The results of these tests were used to obtain an optimized manufacturing process with improved mechanical properties and a lower water uptake after sorption analysis and water immersion. The optimized process parameters allowed us to improve the Young’ Modulus after 30h immersion of 58% and a water uptake reduction of 78% with the first 8 tests done.

PROTEIN INFLUENCE ON THE MECHANICAL PROPERTIES OF NR

2021 ◽  
Author(s):  
Yayoi Akahori ◽  
Misao Hiza ◽  
Soki Yamaguchi ◽  
Seiichi Kawahara
Keyword(s):  
Mechanical Properties ◽  
Nmr Spectroscopy ◽  
Sodium Dodecyl Sulfate ◽  
Carbon Black ◽  
Natural Rubber ◽  
Hevea Brasiliensis ◽  
Sodium Dodecyl ◽  
Swelling Degree ◽  
Deproteinized Natural Rubber ◽  
Rubber State

ABSTRACT Protein effect on vulcanization of NR, obtained from Hevea brasiliensis, was investigated by analyzing the crosslinking structure of the resulting vulcanizates prepared from untreated NR, deproteinized natural rubber (DPNR), and protein-free natural rubber (PFNR) by swelling methods and rubber-state NMR spectroscopy. The proteins present in NR were removed by three methods: deproteinization with enzyme, urea, or urea–acetone in the presence of sodium dodecyl sulfate. The amount of proteins present in NR, approximately 0.238 w/w%, was reduced to 0.000 w/w% by urea–acetone deproteinization, whereas it was reduced to approximately 0.003 and 0.019 w/w% by enzyme and urea deproteinizations, respectively. Hardness, swelling degree, and crosslinking structure depended on the amount of proteins. Changes in mechanical properties for the vulcanizates prepared from not only non-filler compounds but also carbon black–filled and silica-filled compounds were attributed to the amount of proteins.

Decellularization of porcine carotid arteries using low-concentration sodium dodecyl sulfate

2020 ◽  
pp. 039139882097542
Author(s):  
Jin Cheng ◽  
Ji Li ◽  
Zhiwen Cai ◽  
Yuehao Xing ◽  
Cong Wang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Sodium Dodecyl Sulfate ◽  
Carotid Arteries ◽  
Sodium Dodecyl ◽  
Dimensional Structure ◽  
Low Concentration ◽  
Dodecyl Sulfate ◽  
Decellularized Scaffolds ◽  
Triton X

Background: The decellularized scaffold is a promising material for producing tissue-engineered vascular grafts (TEVGs) because of its complex, native-like three-dimensional structure and mechanical properties. Sodium dodecyl sulfate (SDS), one of the most commonly used decellularization reagents, appears to be more effective than other detergents for removing cells from dense tissues. The concentrations of SDS used in previous studies and their effects on decellularization are not consistent. Methods: In this study, porcine carotid arteries were decellularized using detergent-based protocols using Triton X-100 followed by SDS at different concentrations and exposing time. Cell removal efficiency and composition were evaluated by histological analysis, and DNA and collagen quantification. Ultrastructure, mechanical properties, pore size distribution, and in vivo biocompatibility of decellularized arteries were also evaluated. Results: The DNA content of decellularized scaffolds treated with 0.3% SDS for 72 h or 0.5% SDS for 48 h was significantly less than that treated with 1% SDS for 30 h. There was a significant loss of soluble collagen after treatment with 1% SDS relative to native arteries. The extensive loss of elastin and glycosaminoglycans was observed in decellularized arteries treated with 0.5% SDS or 1% SDS. The basement membrane and biomechanics were also damaged by these two protocols. Moreover, decellularized scaffolds became more porous with many large pores after treatment with 0.3% SDS. Conclusion: Low-concentration SDS could be a suitable choice for artery decellularization. Decellularized porcine carotid arteries, prepared using Triton X-100 followed by 0.3% SDS, may be a promising biological scaffold for TEVGs.

The Preparation and Properties of PEN/MWNT Nanocomposites

2010 ◽  
Vol 44 (21) ◽  
pp. 2453-2460 ◽  
Author(s):  
Jiachun Zhong ◽  
Wenjin Chen ◽  
Kun Jia ◽  
Xiaobo Liu
Keyword(s):  
Mechanical Properties ◽  
Carbon Nanotube ◽  
Sodium Dodecyl Sulfate ◽  
Rheological Behavior ◽  
Sodium Dodecyl ◽  
Ultrasound Treatment ◽  
Multiwalled Carbon ◽  
Content Ratio ◽  
Dispersion Agent ◽  
Mwnt Content

Polyarylene ether nitrile (PEN)/multiwalled carbon nanotube (MWNT) composites was prepared, and their rheological behavior and mechanical properties were studied. The PEN/MWNT composites have been fabricated via solution mixing by using ultrasound and dispersion agent, and then casting. This results show that the rheological and mechanical properties of the PEN/MWNT nanocomposites prepared by ultrasound treatment with sodium dodecyl sulfate increase more significantly than that of the ultrasound-treated PEN/MWNT composites and the untreated PEN/MWNT composites with the increase in the MWNT content. The rheological and mechanical properties of the PEN/MWNT composites were related with the content ratio of the MWNT and the preparation ways.

scholarly journals The impact of molded pulp product process on the mechanical properties of molded BCTMP

2020 ◽  
Author(s):  
Claire Dislaire ◽  
Yves Grohens ◽  
Bastien Seantier ◽  
Marion Muzy
Keyword(s):  
Mechanical Properties ◽  
Water Uptake ◽  
Process Parameters ◽  
Manufacturing Process ◽  
Water Immersion ◽  
Young Modulus ◽  
Drying Time ◽  
Pressing Time ◽  
Hygroscopic Properties ◽  
The Impact

Abstract This study was carried out using bleached softwood Chemi-Thermo-Mechanical Pulp to evaluate the influence of Molded Pulp Products’ manufacturing process parameters on the finished products’ mechanical and hygroscopic properties. A Taguchi table was done to make 8 tests with specific process parameters such as moulds temperature, pulping time, drying time and pressing time. The results of these tests were used to obtain an optimized manufacturing process with improved mechanical properties and a lower water uptake after sorption analysis and water immersion. The optimized process parameters allowed us to improve the Young’ Modulus after 1h immersion of 58% and a water uptake reduction of 78% with the first 8 tests done.

The Impact of Electrolyte on the Aggregation of the Complexes of Hyperbranched Poly(ethyleneimine) and Sodium Dodecyl Sulfate

Langmuir ◽  
2009 ◽  
Vol 25 (13) ◽  
pp. 7304-7312 ◽  
Author(s):  
Amália Mezei ◽  
Ágnes Ábrahám ◽  
Katalin Pojják ◽  
Róbert Mészáros
Keyword(s):  
Sodium Dodecyl Sulfate ◽  
Sodium Dodecyl ◽  
Hyperbranched Poly ◽  
Dodecyl Sulfate ◽  
The Impact

scholarly journals Skin Substitute Preparation Method Induces Immunomodulatory Changes in Co-Incubated Cells through Collagen Modification

Pharmaceutics ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 2164
Author(s):  
Jordan Holl ◽  
Cezary Pawlukianiec ◽  
Javier Corton Ruiz ◽  
Dawid Groth ◽  
Kamil Grubczak ◽  
...  
Keyword(s):  
T Cell ◽  
Therapeutic Potential ◽  
Sodium Dodecyl ◽  
Skin Substitute ◽  
Skin Substitutes ◽  
Dermal Matrix ◽  
Beneficial Effects ◽  
Ionic Detergent ◽  
Healing Wounds ◽  
Triton X

Chronic ulcerative and hard-healing wounds are a growing global concern. Skin substitutes, including acellular dermal matrices (ADMs), have shown beneficial effects in healing processes. Presently, the vast majority of currently available ADMs are processed from xenobiotic or cadaveric skin. Here we propose a novel strategy for ADM preparation from human abdominoplasty-derived skin. Skin was processed using three different methods of decellularization involving the use of ionic detergent (sodium dodecyl sulfate; SDS, in hADM 1), non-ionic detergent (Triton X-100 in hADM 2), and a combination of recombinant trypsin and Triton X-100 (in hADM 3). We next evaluated the immunogenicity and immunomodulatory properties of this novel hADM by using an in vitro model of peripheral blood mononuclear cell culture, flow cytometry, and cytokine assays. We found that similarly sourced but differentially processed hADMs possess distinct immunogenicity. hADM 1 showed no immunogenic effects as evidenced by low T cell proliferation and no significant change in cytokine profile. In contrast, hADMs 2 and 3 showed relatively higher immunogenicity. Moreover, our novel hADMs exerted no effect on T cell composition after three-day of coincubation. However, we observed significant changes in the composition of monocytes, indicating their maturation toward a phenotype possessing anti-inflammatory and pro-angiogenic properties. Taken together, we showed here that abdominoplasty skin is suitable for hADM manufacturing. More importantly, the use of SDS-based protocols for the purposes of dermal matrix decellularization allows for the preparation of non-immunogenic scaffolds with high therapeutic potential. Despite these encouraging results, further studies are needed to evaluate the beneficial effects of our hADM 1 on deep and hard-healing wounds.

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