scholarly journals Biosynthesis of Silver Nanoparticles Using Stenocereus queretaroensis Fruit Peel Extract: Study of Antimicrobial Activity

Materials ◽  
2021 ◽  
Vol 14 (16) ◽  
pp. 4543
Author(s):  
Eduardo Padilla-Camberos ◽  
Ivan Moises Sanchez-Hernandez ◽  
Omar Ricardo Torres-Gonzalez ◽  
Patricia Ramirez-Rodriguez ◽  
Emmanuel Diaz ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Foodborne Pathogens ◽  
Cytotoxic Agents ◽  
Minimal Bactericidal Concentration ◽  
Fruit Peel ◽  
Log Reduction ◽  
Gram Positive Bacteria ◽  
Biological Methods ◽  
Time Kill

The synthesis and application of nanomaterials as antioxidants and cytotoxic agents has increased in recent years. Biological methods go beyond the chemical and physical synthesis that is expensive and not friendly to the environment. Foodborne pathogens and microorganisms causing candidiasis are responsible of 5–10% hospitalized patients. The nutritional properties of the fruit called pitaya, from the Stenocereus queretaroensis species, have been little explored. Therefore, in this study the phytochemical composition of S. queretaroensis peel was evaluated and silver nanoparticles (AgNPs) were synthesized biologically in an environmentally friendly way by S. queretaroensis peel aqueous extract that contains phytochemicals capable of reducing silver nitrate. The antimicrobial activity of the AgNPs was tested by determining the minimum inhibitory concentration (MIC), minimal bactericidal concentration (MBC) and time-kill kinetics. AgNPs were characterized visually, by UV-visible spectroscopy and TEM. FTIR spectroscopy identified metabolites responsible for the AgNPs formation. AgNPs showed potent antimicrobial activity against gram-negative and gram-positive bacteria, against fungi, and a methicillin-resistant strain of S. aureus. MIC and MBC values were as low as 0.078 and 0.156 μg/mL using AgNPs biosynthesized by S. queretaroensis fruit peel and the time kill assay started a log reduction in CFU/mL at 1 × MIC and 2 × MIC. S. queretaroensis-mediated AgNPs could be the basis for the formulation of biofilms for packaging products or as disinfectants for use on different surfaces.

scholarly journals Photoactivated Self-Sanitizing Chlorophyllin-Containing Coatings to Prevent Microbial Contamination in Packaged Food

Coatings ◽  
2018 ◽  
Vol 8 (9) ◽  
pp. 328 ◽  
Author(s):  
Gracia López-Carballo ◽  
Pilar Hernández-Muñoz ◽  
Rafael Gavara
Keyword(s):  
Antimicrobial Activity ◽  
High Efficiency ◽  
Antimicrobial Properties ◽  
Methyl Cellulose ◽  
Log Reduction ◽  
Gram Positive Bacteria ◽  
Sodium Magnesium ◽  
Pet Film ◽  
Packaged Food ◽  
Sodium Copper Chlorophyllin

Chlorophyllins are semi-synthetic porphyrins obtained from chlorophyll that—when exposed to visible light—generate radical oxygen substances with antimicrobial activity. In this work, chlorophyllins incorporated with polyethylene (PE), polyvinyl alcohol (PVOH), (hydroxypropyl)methyl cellulose (HPMC), and gelatin (G) were formulated for application as coatings in packages providing antimicrobial activity after photoactivation. First, the antimicrobial properties of two porphyrins (sodium magnesium chlorophyllin, E-140, and sodium copper chlorophyllin, E-141) were analyzed against L. monocytogenes and Escherichia coli. The results indicated that E-140 was more active than E-141 and that chlorophyllins were more effective against Gram-positive bacteria. In addition, both chlorophyllins were more efficient when irradiated with halogen lamps than with LEDs, and they were inactive in dark conditions. Then, coatings on polyethylene terephthalate (PET) film were prepared, and their effect against the test bacteria was similar to that shown previously with pure chlorophyllins, i.e., greater activity in films containing E-140. Among the coating matrices, those based on PE presented the least effect (1 log reduction), whereas PVOH, HPMC, and G were lethal (7 log reduction). The self-sanitizing effect of these coatings was also analyzed by contaminating the surface of the coatings and irradiating them through the PET surface, which showed high efficiency, although the activity of the coatings was limited to L. monocytogenes. Finally, coated films were applied as separators of bologna slices. After irradiation, all the films showed count reductions of L. monocytogenes and the usual microbial load; the gelatin coating was the most effective, with an average of 3 log reduction.

Optimization of Silver Nanoparticles Production using Aspergillus niger and Study of Antimicrobial Activity

2021 ◽  
Vol 12 (4) ◽  
pp. 2383-2388
Author(s):  
Suguna Selvakumaran ◽  
Kayathri Marimuthu ◽  
Thiruvany Poopalan ◽  
Kalaiyarasi Tamil Selvan ◽  
Nozieana Khairuddin
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Aspergillus Niger ◽  
Culture Supernatant ◽  
Gram Negative Bacteria ◽  
Mixing Ratio ◽  
Zone Of Inhibition ◽  
Gram Positive Bacteria ◽  
Physiochemical Parameters ◽  
Uv Visible

Silver nanoparticles have attracted high attention worldwide for their various applications. The physiochemical parameters such as temperature, media, mixing ratio affect the rate of synthesis of silver nanoparticles and their yield. Thus, optimization of these physiochemical parameters is needed to enhance the production of silver nanoparticles. In this study, silver nanoparticles were synthesized using Aspergillus niger culture supernatant. The produced silver nanoparticles were characterized using UV-visible Spectrophotometer at 200 nm to 700 nm, which had a peak at 450 nm, indicates the formation of silver nanoparticles. It was found that Sabouraud Dextrose Broth (SDB) as optimum media, 40 ml of supernatant and 10 ml of silver nitrate as optimum mixing ratio and 65°C as optimum temperature to produce silver nanoparticles. The optimized silver nanoparticles were subjected to antimicrobial activity, and it was found that it is highly effective towards gram-negative bacteria than gram-positive bacteria where the zone of inhibition for Escherichia coli was  7 ± 2.7 mm and 5.3 ± 2.1 mm for Staphylococcus aureus.

Fruit peels waste for the green synthesis of silver nanoparticles with antimicrobial activity against foodborne pathogens

LWT ◽  
2019 ◽  
Vol 103 ◽  
pp. 293-300 ◽  
Author(s):  
Karen M. Soto ◽  
Camila T. Quezada-Cervantes ◽  
Montserrat Hernández-Iturriaga ◽  
Gabriel Luna-Bárcenas ◽  
Rafael Vazquez-Duhalt ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Foodborne Pathogens

scholarly journals Characterization and Application of Enterocin RM6, a Bacteriocin fromEnterococcus faecalis

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
En Huang ◽  
Liwen Zhang ◽  
Yoon-Kyung Chung ◽  
Zuoxing Zheng ◽  
Ahmed E. Yousef
Keyword(s):  
Mass Spectrometry ◽  
Foodborne Pathogens ◽  
High Performance ◽  
Cyclic Peptide ◽  
Food Preservation ◽  
Peptide Sequence ◽  
Cottage Cheese ◽  
Log Reduction ◽  
Gram Positive Bacteria ◽  
Potential Applications

Use of bacteriocins in food preservation has received great attention in recent years. The goal of this study is to characterize enterocin RM6 fromEnterococcus faecalisOSY-RM6 and investigate its efficacy againstListeria monocytogenesin cottage cheese. Enterocin RM6 was purified fromE. faecalisculture supernatant using ion exchange column, multiple C18-silica cartridges, followed by reverse-phase high-performance liquid chromatography. The molecular weight of enterocin RM6 is 7145.0823 as determined by mass spectrometry (MS). Tandem mass spectrometry (MS/MS) analysis revealed that enterocin RM6 is a 70-residue cyclic peptide with a head-to-tail linkage between methionine and tryptophan residues. The peptide sequence of enterocin RM6 was further confirmed by sequencing the structural gene of the peptide. Enterocin RM6 is active against Gram-positive bacteria, includingL. monocytogenes,Bacillus cereus,and methicillin-resistantStaphylococcus aureus(MRSA). Enterocin RM6 (final concentration in cottage cheese, 80 AU/mL) caused a 4-log reduction in population ofL. monocytogenesinoculated in cottage cheese within 30 min of treatment. Therefore, enterocin RM6 has potential applications as a potent antimicrobial peptide against foodborne pathogens in food.

scholarly journals Effect of Dispersion Solvent on the Deposition of PVP-Silver Nanoparticles onto DBD Plasma-Treated Polyamide 6,6 Fabric and Its Antimicrobial Efficiency

Nanomaterials ◽  
2020 ◽  
Vol 10 (4) ◽  
pp. 607
Author(s):  
Ana I. Ribeiro ◽  
Martina Modic ◽  
Uros Cvelbar ◽  
Gheorghe Dinescu ◽  
Bogdana Mitu ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Photoelectron Spectroscopy ◽  
Antimicrobial Effect ◽  
Dbd Plasma ◽  
Surface Distribution ◽  
E Coli ◽  
Log Reduction ◽  
Spray Method ◽  
Exhaustion Method

Polyvinylpyrrolidone-coated silver nanoparticles (PVP-AgNPs) dispersed in ethanol, water and water/alginate were used to functionalize untreated and dielectric barrier discharge (DBD) plasma-treated polyamide 6,6 fabric (PA66). The PVP-AgNPs dispersions were deposited onto PA66 by spray and exhaustion methods. The exhaustion method showed a higher amount of deposited AgNPs. Water and water-alginate dispersions presented similar results. Ethanol amphiphilic character showed more affinity to AgNPs and PA66 fabric, allowing better uniform surface distribution of nanoparticles. Antimicrobial effect in E. coli showed good results in all the samples obtained by exhaustion method but using spray method only the DBD plasma treated samples displayed antimicrobial activity (log reduction of 5). Despite the better distribution achieved using ethanol as a solvent, water dispersion samples with DBD plasma treatment displayed better antimicrobial activity against S. aureus bacteria in both exhaustion (log reduction of 1.9) and spray (methods log reduction of 1.6) due to the different oxidation states of PA66 surface interacting with PVP-AgNPs, as demonstrated by X-ray Photoelectron Spectroscopy (XPS) analysis. Spray method using the water-suspended PVP-AgNPs onto DBD plasma-treated samples is much faster, less agglomerating and uses 10 times less PVP-AgNPs dispersion than the exhaustion method to obtain an antimicrobial effect in both S. aureus and E. coli.

Green Synthesis of Silver Nanoparticle Using Banana (Musa) Sap

2015 ◽  
Vol 1086 ◽  
pp. 7-10 ◽  
Author(s):  
S. Deena ◽  
Arun Dakshinamurthy ◽  
Paulraj Mosae Selvakumar
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Green Synthesis ◽  
Silver Nanoparticle ◽  
Gram Negative Bacteria ◽  
Visible Spectroscopy ◽  
High Antimicrobial Activity ◽  
Gram Positive Bacteria ◽  
Coated Fabric ◽  
Uv Visible

Green synthesis of silver nanoparticles using banana sap as a solvent, reducing agent and capping agent is reported in this work. Banana sap has also been used as a binder to incorporate silver nanoparticles into cotton fabric. UV-Visible spectroscopy was used to monitor the quantitative formation of silver nanoparticles. Silver nanoparticle coated fabric showed high antimicrobial activity against gram negative bacteria such asPseudomonas aureginosaandEscherichia coli, and gram positive bacteria such asBacillus subtilisandStaphylococcus aureus.KeywordsGreen synthesis, Silver nanoparticles, banana sap, antimicrobial activity

scholarly journals SYNTHESIS AND ANTIMICROBIAL ACTIVITY OF SILVER NANOPARTICLES STABILIZED BY CITRATE ANIONS

2020 ◽  
Vol 2020 (60) ◽  
pp. 127-135
Author(s):  
Liliya BAZYLYAK ◽  
◽  
Andriy KYTSYA ◽  
Ilona KARPENKO ◽  
Olena KARPENKO ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Cell Walls ◽  
Colloidal Silver ◽  
Gram Negative Bacteria ◽  
Gram Positive ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Vis Spectroscopy

Widespread use of synthetic antimicrobial drugs leads to the development of antibiotic resistance of pathogenic strains of microorganisms. Therefore, today researchers are very interested in drugs based on nanoparticles of metals, in particular silver and copper, which have antibacterial, antifungal and antiviral activity. One of the reasons for the high interest of researchers in AgNPs as an antimicrobial agent is the significantly lower toxicity of AgNPs compared to Ag+ ions. High antibacterial efficiency of silver nano¬particles is achieved due to their developed surface, which provides maximum contact with the environment. In addition, such nanoparticles are quite small and are able to penetrate cell membranes, to affect intracellular processes from within. Therefore, the aim of this work was to obtain concentrated colloidal silver solutions stabilized by citrate anions, which simultaneously provide satisfactory stabilization of colloidal silver solutions and are non-toxic, as well as to investigate the antimicrobial action of synthesized AgNPs. The solution of citrate stabilized silver nanoparticles (AgNPs) have been obtained via the reaction of reduction of silver nitrate by hydrazine in alkaline medium in the presence of sodium citrate. AgNPs were investigated using transmission electron microscopy (TEM) and UV-vis spectroscopy and the particles size and particles size distribution (PSD) were determined. It was observed that obtained AgNPs are mainly spherical shape. It was found that the mean diameter and PSD of AgNPs determined using TEM and UV-vis spectroscopy are close and equal to 14 and 5 nm and 15 and 4 nm respectively. Obtained solution was concentrated by evaporation at 70 C under reduced pressure up to achievement of AgNPs concentration equal to 200 mg/L. On the base of comparison of optical properties of initial silver sol and concentrated solution the minority of agglomeration of AgNPs was statement. At the same time AgNO3 test showed no change of UV-vis spectrum of concentrate that points on the absence of reducing agent in the solution; this fact indicate that hydrazine was eliminated from during the evaporation of initial AgNPs solution and obtained concentrate did not consist the toxic impurities. Antimicrobial activity of obtained citrate stabilised AgNPs against Gram-positive Bacillus subtilis and Gram-negative Escherichia coli bacterium was tested using disk diffusion method. It was found that AgNPs shown significant bactericidal effect even at low (25 mg/L) concentration as well as some higher efficiency against Gram-negative bacterium. There was also a slightly higher antimicrobial activity of the drug against gram-negative bacteria Escherichia coli compared with gram-positive bacteria Bacillus subtilis, due to the different structure of cell walls. In particular, the walls of gram-positive bacteria consist mainly of peptidoglycan (murein), and gram-negative bacteria have cell walls with a layer of peptidoglycan and an outer membrane with a lipopolysaccharide component, which is not present in gram-positive bacteria. Based on the studies, it can be concluded that the proposed method of synthesis of AgNPs is suitable for obtaining highly concentrated silver sols. This method of synthesis is simple in hardware design, scalable, and the resulting colloidal solutions are stable and do not contain harmful impurities. Therefore, due to the high antibacterial activity of citrate-anion-stabilized AgNPs against certain types of gram-positive and gram-negative bacteria, it can be recommended for the manufacture of bactericidal drugs for biomedical purposes.

scholarly journals Green Synthesis, Antioxidant and Antimicrobial Activity of Silver Nanoparticles Using Gnetum africanum Extracts

2021 ◽  
pp. 1-7
Author(s):  
I. M. Uneze ◽  
J. O. Otonko ◽  
A. K. Adigun ◽  
S. J. Adebayo
Keyword(s):  
Escherichia Coli ◽  
Aqueous Solution ◽  
Staphylococcus Aureus ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Silver Nitrate ◽  
Leaf Extract ◽  
Gram Negative Bacteria ◽  
Gram Negative ◽  
Gram Positive Bacteria

The synthesis and application of nanoparticles is an important area of research that is gaining attention recently. In this recent project, we report the synthesis of silver nanoparticles, AgNP using aqueous solution of silver nitrate and Gnetum africanum leaf extract (reducing agent). The synthesis of AgNP was achieved by mixing aqueous solution of silver nitrate (70ml, 15.75mM) with a solution of Gnetum africanum leaf extract 100 ml) in a reaction flask and allowed to stand for 24 hours in a dark cupboard. A color change from light brown to yellowish brown was observed which indicated that synthesis of silver nanoparticles took place. The presence of AgNP was ascertained using UV-vis spectra analysis and absorption at 442 nm showed the presence of AgNP. The antioxidant assay of both the synthesized AgNP and the leaf extract was determined using DPPH. Antimicrobial activity was conducted using three different organisms which were Staphylococcus aureus, Escherichia coli and Pseudomonas respectively. The antioxidant results using DPPH scavenging ability of AgNp showed that at concentrations of 2mg/ml,1mg/ml and 0.1mg/ml, the percentage inhibition  of  DPPH  by AgNp was 61.69, 53.06 and 38.31 respectively and that of Gnetum africanum leaf extract was 81.32, 78.49, and 58.29 respectively at the same concentrations using Ascorbic acid as a standard. The antimicrobial activity of both the synthesized AgNps and Gnetum Africanum Leaf extract using one gram positive bacteria (Staphylococcus aureus) and two gram negative bacteria (Escherichia coli and Pseudomonas) revealed that the synthesized AgNps showed lesser activity than Gnetumafricanum leaf extract for both the gram positive bacteria (Staphylococcus aureus) and gram negative bacteria (Pseudomonas) and (Escherichia coli). From the above findings, it can be observed that Gnetum Africanum Leaf extract reduced Ag+ to Ag0 and also both the synthesized AgNps and the Gnetum Africanum Leaf extract showed reasonable antioxidant activity against DPPH and antimicrobial activity against the tested microorganisms. This implied that both samples have medicinal values.

scholarly journals Silver nanoparticles incorporated into nanostructured biopolymer membranes produced by electrospinning: a study of antimicrobial activity

2015 ◽  
Vol 51 (4) ◽  
pp. 911-921 ◽  
Author(s):  
Karen Segala ◽  
Silvia Vaz Guerra Nista ◽  
Lívia Cordi ◽  
Maria Trindade Marques Bizarria ◽  
José de Ávila Júnior ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Escherichia Coli ◽  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Propionibacterium Acnes ◽  
Electrospun Nanofibers ◽  
Gram Positive ◽  
Gram Positive Bacteria ◽  
Nanostructured Membranes ◽  
Average Size

abstract This study examines the antimicrobial activity of silver nanoparticles incorporated into nanostructured membranes made of cellulose acetate (CA) and blends of chitosan/poly-(ethylene oxide, CTS/PEO) and prepared by electrospinning. The formation of chemically synthesized Ag nanoparticles (AgNPs) was monitored by UV-visible spectroscopy (UV-Vis) and characterized by transmission electron microscopy (TEM). The size distribution of the AgNPs was measured by dynamic light scattering (DLS), with an average size of approximately 20 nm. The presence of AgNPs on the surface of electrospun nanofibers was observed by field emission electron microscopy (FEG) and confirmed by TEM. The antimicrobial activity of AgNPs incorporated into nanostructured membranes made of CA and CTS/PEO electrospun nanofibers was evaluated in the presence of both Gram-positive bacteria, such as Staphylococcus aureus ATCC 29213 and Propionibacterium acnes ATCC 6919, and Gram-negative bacteria, such as Escherichia coli ATCC 25992 and Pseudomonas aeruginosa ATCC 17933. Microbiological results showed that the presence of AgNPs in CA and CTS/PEO nanostructured membranes has significant antimicrobial activity for the Gram-positive bacteria Escherichia coli and Propionibacterium acnes.

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