scholarly journals Photoactivated Self-Sanitizing Chlorophyllin-Containing Coatings to Prevent Microbial Contamination in Packaged Food

Coatings ◽  
2018 ◽  
Vol 8 (9) ◽  
pp. 328 ◽  
Author(s):  
Gracia López-Carballo ◽  
Pilar Hernández-Muñoz ◽  
Rafael Gavara
Keyword(s):  
Antimicrobial Activity ◽  
High Efficiency ◽  
Antimicrobial Properties ◽  
Methyl Cellulose ◽  
Log Reduction ◽  
Gram Positive Bacteria ◽  
Sodium Magnesium ◽  
Pet Film ◽  
Packaged Food ◽  
Sodium Copper Chlorophyllin

Chlorophyllins are semi-synthetic porphyrins obtained from chlorophyll that—when exposed to visible light—generate radical oxygen substances with antimicrobial activity. In this work, chlorophyllins incorporated with polyethylene (PE), polyvinyl alcohol (PVOH), (hydroxypropyl)methyl cellulose (HPMC), and gelatin (G) were formulated for application as coatings in packages providing antimicrobial activity after photoactivation. First, the antimicrobial properties of two porphyrins (sodium magnesium chlorophyllin, E-140, and sodium copper chlorophyllin, E-141) were analyzed against L. monocytogenes and Escherichia coli. The results indicated that E-140 was more active than E-141 and that chlorophyllins were more effective against Gram-positive bacteria. In addition, both chlorophyllins were more efficient when irradiated with halogen lamps than with LEDs, and they were inactive in dark conditions. Then, coatings on polyethylene terephthalate (PET) film were prepared, and their effect against the test bacteria was similar to that shown previously with pure chlorophyllins, i.e., greater activity in films containing E-140. Among the coating matrices, those based on PE presented the least effect (1 log reduction), whereas PVOH, HPMC, and G were lethal (7 log reduction). The self-sanitizing effect of these coatings was also analyzed by contaminating the surface of the coatings and irradiating them through the PET surface, which showed high efficiency, although the activity of the coatings was limited to L. monocytogenes. Finally, coated films were applied as separators of bologna slices. After irradiation, all the films showed count reductions of L. monocytogenes and the usual microbial load; the gelatin coating was the most effective, with an average of 3 log reduction.

Antimicrobial Properties of Composites of Chitosan-Silver Doped Zeolites

2020 ◽  
Vol 20 (10) ◽  
pp. 6295-6304
Author(s):  
Fabio A. P. Scacchetti ◽  
Filipa Fernandes ◽  
Artur Ribeiro ◽  
Artur Cavaco-Paulo ◽  
Joaquim O. Carneiro ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Sodium Tripolyphosphate ◽  
Antimicrobial Properties ◽  
X Ray Diffraction ◽  
Gram Negative ◽  
X Ray ◽  
Gram Positive Bacteria ◽  
Ph Conditions ◽  
Average Size ◽  
Properties Of Composites

The antimicrobial functionality of composites constituted by chitosan with silver-doped zeolites was developed and characterized. A composite with chitosan particles and silver-doped zeolites was synthesized using an ionic gelation process with sodium tripolyphosphate. The chitosan silver-doped zeolites composite obtained presented sizes up to 5 μm, while the silver-doped zeolites had an average size between 0.5 μm and 3.3 μm. The synthesized chitosan silver-doped zeolites composites, as well as the silver-doped zeolites and the chitosan dissolution, were characterized through X-ray diffraction, Fourier Transform Infrared spectroscopy and scanning electron microscopy. The electro kinetic behaviour of chitosan, silver-doped zeolites and chitosan silver-doped zeolites composite was evaluated under different pH conditions. The antimicrobial activity of the composites was evaluated in terms of minimum inhibitory concentrations and minimum lethal concentrations and the results suggest that the chitosan silver-doped zeolites composites show antimicrobial activity against gram-negative and gram-positive bacteria, Escherichia coli and Staphylococcus aureus, respectively and against Candida albicans. The results here presented support the potential application of the composite of chitosan with silver-doped zeolites in the functionalization of textiles with antimicrobial properties.

scholarly journals ANTIMICROBIAL ACTIVITY AND MINIMUM EFFECTIVE CONCENTRATIONS OF CHEMICAL COMPOUNDS FOUND IN DISINFECTANTS

2019 ◽  
Vol 98 (10) ◽  
pp. 1031-1036
Author(s):  
Nikolay V. Shestopalov ◽  
L. S. Fedorova ◽  
A. Yu. Skopin
Keyword(s):  
Antimicrobial Activity ◽  
Antimicrobial Properties ◽  
Chemical Compounds ◽  
Effective Concentration ◽  
Human Welfare ◽  
Minimum Effective Concentration ◽  
Gram Negative ◽  
Gram Positive Bacteria ◽  
Rights Protection ◽  
Consumer Rights

The antimicrobial properties of various chemical compounds are significantly different. These differences are determined as the properties of the chemical compound and the properties of applied microorganisms. To determine the antimicrobial activity and establish the minimum effective concentrations of the most common chemical compounds, the Research Institute for Disinfectology of the Federal Service on Consumer Rights Protection and Human Welfare Supervision Rospotrebnadzor conducted extensive research. The antimicrobial properties of chemical compounds were studied in relation to the main test microorganisms - gram-negative and gram-positive bacteria, fungi, mold, viruses. The results obtained allowed establishing the minimum effective concentration for 9 chemical compounds in relation to 10 test microorganisms.

scholarly journals UV Curable Coatings Based on Urethane Acrylates Containing Eugenol and Evaluation of Their Antimicrobial Activity

Coatings ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1556
Author(s):  
Paulina Bednarczyk ◽  
Agnieszka Wróblewska ◽  
Agata Markowska-Szczupak ◽  
Paula Ossowicz-Rupniewska ◽  
Małgorzata Nowak ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Protective Coatings ◽  
Antimicrobial Properties ◽  
Uv Curing ◽  
Industrial Applications ◽  
High Reactivity ◽  
Uv Curable ◽  
Gram Positive Bacteria ◽  
Wide Range ◽  
Short Time

This work presents studies on the obtaining of UV-curable coatings with antimicrobial activity. Urethane acrylates (UAs) have excellent physicochemical properties including high reactivity in systems with radical photoinitiators in the presence of UV radiation and good balance between hardness and flexibility in the formed coatings. At the same time, eugenol is well known as the compound hindering the growth of various microorganisms. Hence, the materials obtained by the modification of UA resins with eugenol can be used to protect various surfaces, especially against microorganisms. This study aimed to examine the influence of the amount of eugenol on the chemical, physical, thermal, and mechanical properties of the obtained UA coatings and find the conditions at which the optimal properties for industrial applications such coatings can be achieved. These materials were successfully obtained. Taking into account that eugenol is a very cheap reactant, and it can be obtained from natural sources by the simple distillation method, the proposed method combined the good points of obtaining protective coatings by UV curing with the utilization of vegetable, renewable reactants (biomass), such as components giving special properties to these materials, in this case, antimicrobial properties. In this study, photoreactive coatings with antimicrobial properties for the following microorganisms: fungi (C. albicans), Gram-positive bacteria (S. epidermidis) as well as Gram-negative bacteria (E. coli), were obtained. The obtained coatings were cured over a short time. They were colorless and characterized by a wide range of properties and applications.

Chemical Composition and Antimicrobial Activity of Teucrium Capitatum L. Subsp. Lusitanicum (Schreb.) T. Navarro & Rosua Essential Oil

2020 ◽  
Author(s):  
Haïfa Debbabi ◽  
Ridha El Mokni ◽  
Ibrahim Jlassi ◽  
Rajesh K Joshi ◽  
Saoussen Hammami
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Antimicrobial Properties ◽  
Significant Activity ◽  
Bacterial Strains ◽  
Gram Positive Bacteria ◽  
Sesquiterpene Hydrocarbons ◽  
Germacrene D

Abstract Chemical composition and antimicrobial activity of Teucrium capitatum L. subsp. lusitanicum essential oil was investigated for the first time in the present study. Qualitative and quantitative analyses of the chemical composition by gas chromatography and mass spectrometry (GC–FID and GC–MS) revealed the presence of 60 compounds representing 97.6% of the whole constituents. The main compounds were germacrene D (47.1%), spathulenol (5.8%), α-selinene (5.3%), germacrene A (2.9%), δ-cadinene (2.8%) and cubenol (2.7%). In vitro, the antimicrobial activity was investigated against five bacterial strains along with the yeast Candida albicans using broth microdilution assay. T. capitatum subsp. lusitanicum essential oil showed significant activity against the gram-positive bacteria Staphylococcus aureus (MIC = MBC = 78 μg mL−1), Bacillus subtilis (MIC = MBC = 156 μg mL−1) and the yeast C. albicans (MIC = MFC = 156 μg mL−1). The great potential of antimicrobial effects is most likely due to the very high percentage of sesquiterpene hydrocarbons particularly to germacrene D, for which the antimicrobial properties have been previously reported.

scholarly journals Biosynthesis of Silver Nanoparticles Using Stenocereus queretaroensis Fruit Peel Extract: Study of Antimicrobial Activity

Materials ◽  
2021 ◽  
Vol 14 (16) ◽  
pp. 4543
Author(s):  
Eduardo Padilla-Camberos ◽  
Ivan Moises Sanchez-Hernandez ◽  
Omar Ricardo Torres-Gonzalez ◽  
Patricia Ramirez-Rodriguez ◽  
Emmanuel Diaz ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Silver Nanoparticles ◽  
Foodborne Pathogens ◽  
Cytotoxic Agents ◽  
Minimal Bactericidal Concentration ◽  
Fruit Peel ◽  
Log Reduction ◽  
Gram Positive Bacteria ◽  
Biological Methods ◽  
Time Kill

The synthesis and application of nanomaterials as antioxidants and cytotoxic agents has increased in recent years. Biological methods go beyond the chemical and physical synthesis that is expensive and not friendly to the environment. Foodborne pathogens and microorganisms causing candidiasis are responsible of 5–10% hospitalized patients. The nutritional properties of the fruit called pitaya, from the Stenocereus queretaroensis species, have been little explored. Therefore, in this study the phytochemical composition of S. queretaroensis peel was evaluated and silver nanoparticles (AgNPs) were synthesized biologically in an environmentally friendly way by S. queretaroensis peel aqueous extract that contains phytochemicals capable of reducing silver nitrate. The antimicrobial activity of the AgNPs was tested by determining the minimum inhibitory concentration (MIC), minimal bactericidal concentration (MBC) and time-kill kinetics. AgNPs were characterized visually, by UV-visible spectroscopy and TEM. FTIR spectroscopy identified metabolites responsible for the AgNPs formation. AgNPs showed potent antimicrobial activity against gram-negative and gram-positive bacteria, against fungi, and a methicillin-resistant strain of S. aureus. MIC and MBC values were as low as 0.078 and 0.156 μg/mL using AgNPs biosynthesized by S. queretaroensis fruit peel and the time kill assay started a log reduction in CFU/mL at 1 × MIC and 2 × MIC. S. queretaroensis-mediated AgNPs could be the basis for the formulation of biofilms for packaging products or as disinfectants for use on different surfaces.

scholarly journals Antimicrobial Properties of Water-Soluble Polysaccharides and Alcoholic Extracts of Laetiporus Sulphureus (Bull.) Murrill Mycelium and Development of Biotechnology for Its Production in Immobilized Culture on Bacterial Cellulose

2020 ◽  
Vol 65 (1-2) ◽  
pp. 10-14
Author(s):  
I. A. Gavryushina ◽  
T. I. Gromovykh ◽  
N. B. Feldman ◽  
S. V. Lutsenko ◽  
V. I. Ponomarenko ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Bacterial Cellulose ◽  
Synthetic Medium ◽  
Antibacterial Properties ◽  
Antimicrobial Properties ◽  
Water Soluble ◽  
Biologically Active ◽  
Aqueous Extracts ◽  
Gram Positive Bacteria ◽  
Immobilized Culture

The article discusses the development of a new method of producing Laetiporus sulphureus (Bull.) Murrill basidiomycete mycelium immobilized on a bacterial cellulose matrix. Mycelium contains biologically active compounds with antimicrobial activity against Gram-positive bacteria, including resistant staphylococcus. The aim of the work was to obtain immobilized mycelium by co-cultivation of L.sulphureus with the producer of bacterial cellulose Gluconacetobacter hansenii. The authors found that when co-culturing the basidial L.sulphureus strain with the bacterial cellulose producing G.hansenii strain, productivity increases by 3.2 times on H5/1 synthetic medium and by 1.9 times on natural Maltax-10 medium (concentration 5%). The resulting immobilized L.sulphureus mycelium has antibacterial properties; its aqueous extracts contain glucans.

scholarly journals Essential oils of Thymus pulegioides and Thymus glabrescens from Romania: Chemical composition and antimicrobial activity

2010 ◽  
Vol 75 (1) ◽  
pp. 27-34 ◽  
Author(s):  
Mariana Pavel ◽  
Mihailo Ristic ◽  
Tatjana Stevic
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Micrococcus Luteus ◽  
Antimicrobial Properties ◽  
Herbal Drug ◽  
Qualitative And Quantitative Analysis ◽  
Gram Positive Bacteria ◽  
Main Components

The aim of this work was to analyse the chemical composition and antimicrobial properties of essential oils isolated from two wild-growing species of thyme (Thymus pulegioides L. and T. glabrescens Willd.) originating from different locations in Romania. The yield of essential oil was determined according to European Pharmacopoeia standards. Qualitative and quantitative analysis of the oils was performed using GC and GC/MS. The antimicrobial activity was tested by the microdilution technique against Gram-negative and Gram-positive bacteria (Escherichia coli, Salmonella typhimurium, S. enteritisdis, Enterobacter cloacae, Pseudomonas aeruginosa, Proteus mirabilis, Staphylococcus aureus, S. epidermidis, Streptococcus faecalis, Bacillus subtilis, Micrococcus luteus, M. flavus and Listeria monocytogenes) and human pathogen yeast Candida albicans. The essential oil of Thymus pulegioides was obtained in a yield of 0.7-1 % (v/d.w. herbal drug) and the main components were carvacrol (50.5-62.6 %), ?-terpinene (9.8-9.9 %) and p-cymene (5.8-7.1 %). The essential oil of T. glabrescens was obtained in a yield of 0.7 (v/d.w. herbal drug) and the main components were geraniol (55.5 %), neryl acetate (11.1 %) and ?-bisabolene (6.7 %). The essential oils inhibited microbial growth at concentrations of 10.8-27 ?l/ml.

scholarly journals Antimicrobial Activity of Lysozyme against Oral Pathogens

2016 ◽  
Vol 8 (2) ◽  
pp. 42 ◽  
Author(s):  
T. Jesse Joel ◽  
S. Suguna ◽  
S. R. Steffi
Keyword(s):  
Antimicrobial Activity ◽  
Polyacrylamide Gel Electrophoresis ◽  
Antimicrobial Properties ◽  
Natural Phenomenon ◽  
Diffusion Method ◽  
Antimicrobial Substances ◽  
Gram Positive Bacteria ◽  
Antiviral Compounds ◽  
Human Sample ◽  
Oral Pathogens

Saliva contains a number of antimicrobial substances which have their origin either in salivary glands or serum from which they may leak into the mouth via the gingival crevices. Saliva is believed to possess a substantial influence on the aggregates oral pathogens form in the mouth due to the antibodies as well as proteins whose antimicrobial properties do not depend on prior exposure to an antigen. One such enzyme is Lysozyme which is one of the most powerful natural antibacterial and antiviral compounds known to man. According to previous researches, it has been found out that lysozyme may bind and aggregate Gram-positive bacteria such as putative Gram-negative periodontopathic bacteria such as <em>Capnocytophaga gingivalis</em>. This particular study explores the natural phenomenon Lysozyme exhibits against oral microflora with noteworthy conclusions. Saliva samples were collected from the oral cavity of human, cow and dog. From these samples, lysozyme was isolated and purified. Furthermore, the quantity of the enzyme isolated was ascertained by Polyacrylamide Gel Electrophoresis. The quantified enzyme showed 32kDa for cow sample, 23kDa for dog sample and 23kDa for human sample. Finally the antimicrobial activity of the enzyme was determined by agar well diffusion method against <em>Staphylococcus</em> spp. and <em>Enterococcus faecalis</em>. Hence lysozyme in saliva is found to have the antibacterial activity against the pathogen due to the zone of inhibition observed and this proves that a tool to prevent dental decay is available and that there is potential to serve antimicrobial role in the specific application of medical industry.

PRELIMINARY STUDY OF ANTIMICROBIAL ACTIVITY OF THE SKIN SECRETIONS OF MALAYSIAN FROGS

2016 ◽  
Vol 78 (6-5) ◽  
Author(s):  
Zainon A. Rahman ◽  
Mohamad Faiz Foong Abdullah ◽  
Wan Mohamad Syahmin Wan Azmi ◽  
Aziyah Abdul-Aziz
Keyword(s):  
Antimicrobial Activity ◽  
Bioactive Compounds ◽  
Frog Skin ◽  
Antimicrobial Properties ◽  
Antimicrobial Effect ◽  
Skin Secretion ◽  
Gram Positive Bacteria ◽  
Microbial Infections ◽  
Skin Secretions ◽  
Preliminary Study

Bioactive compounds that exhibit antimicrobial properties in frogs are parts of the animal defense against microbial infections.  The lyophilized frog’s skin secretions containing varies bioactive compounds were subjected to screen for their antimicrobial activity. This study was conducted as part of an effort on the search of antimicrobial peptides (AMPs) profiles of Malaysian frogs. The results indicate the collected frog skin secretion has antimicrobial effect against Gram-negative and Gram-positive bacteria.  BLAST and standard phylogenetics were used to establish a preliminary identity of the frog samples.

scholarly journals Peppermint Essential Oil-Doped Hydroxyapatite Nanoparticles with Antimicrobial Properties

Molecules ◽  
2019 ◽  
Vol 24 (11) ◽  
pp. 2169 ◽  
Author(s):  
Monica Luminita Badea ◽  
Simona Liliana Iconaru ◽  
Andreea Groza ◽  
Mariana Carmen Chifiriuc ◽  
Mircea Beuran ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Candida Parapsilosis ◽  
Antimicrobial Properties ◽  
Gram Negative Bacteria ◽  
Hydroxyapatite Nanoparticles ◽  
E Coli ◽  
Gram Positive Bacteria ◽  
Ellipsoidal Shape ◽  
Ir Bands

This study aimed at developing an antimicrobial material based on hydroxyapatite (HAp) and peppermint essential oil (P-EO) in order to stimulate the antimicrobial activity of hydroxyapatite. The molecular spectral features and morphology of the P-EO, HAp and hydroxyapatite coated with peppermint essential oil (HAp-P) were analyzed using Fourier-transform infrared (FTIR) spectroscopy and scanning electron microscopy (SEM). The coating of the HAp with the P-EO did not affect the ellipsoidal shape of the nanoparticles. The overlapping of IR bands of P-EO and HAp in the HAp-P spectrum determined the formation of the broad molecular bands that were observed in the spectral regions of 400–1000 cm−1 and 1000–1200 cm−1. The antibacterial activity of the P-EO, HAp and HAp-P were also tested against different Gram-positive bacteria (methicillin-resistant Staphylococcus aureus (MRSA) 388, S. aureus ATCC 25923, S. aureus ATCC 6538, E. faecium DSM 13590), Gram-negative bacteria (Escherichia coli ATCC 25922, E. coli C5, P. aeruginosa ATCC 27853, P. aeruginosa ATCC 9027) and a fungal strain of Candida parapsilosis. The results of the present study revealed that the antimicrobial activity of HAp-P increased significantly over that of HAp.

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