scholarly journals Rosehip Extract-Functionalized Magnesium Hydroxide Nanoparticles and Its Effect on Osteoblastic and Osteoclastic Cells

Materials ◽  
2021 ◽  
Vol 14 (15) ◽  
pp. 4172
Author(s):  
Laura Costa Pinho ◽  
Thais Francini Garbieri ◽  
Liliana Grenho ◽  
Marta M. Alves ◽  
Pedro Sousa Gomes ◽  
...  
Keyword(s):  
Bone Metabolism ◽  
Magnesium Hydroxide ◽  
Pure Water ◽  
Cell Types ◽  
Xrd Analysis ◽  
Osteoblastic Differentiation ◽  
Tartrate Resistant Acid Phosphatase ◽  
Alp Activity ◽  
Mean Diameter ◽  
Magnesium Hydroxide Nanoparticles

Considering the role of magnesium in bone metabolism and the increasing relevance of plant-mediated green-synthesis, this work compares the bone cytocompatibility of magnesium hydroxide nanoparticles (NPs) produced by using pure water, Mg(OH)2, or a rosehip (RH) aqueous extract, Mg(OH)2RH. The NPs were evaluated for dose- and time-dependent effects on human osteoblastic and osteoclastic response, due to the direct involvement of the two cell types in bone metabolism. Mg(OH)2 NPs presented nanoplatelet-like morphology (mean diameter ~90 nm) and a crystalline structure (XRD analysis); the RH-mediated synthesis yielded smaller rounded particles (mean diameter <10 nm) with decreased crystallinity. On the ATR–FTIR spectra, both NPs presented the characteristic Mg-OH peaks; Mg(OH)2RH exhibited additional vibration bands associated with the presence of phytochemicals. On osteoblastic cells, NPs did not affect cell growth and morphology but significantly increased alkaline phosphatase (ALP) activity; on osteoclastic cells, particles had little effect in protein content, tartrate-resistant acid phosphatase (TRAP) activity, percentage of multinucleated cells, and cell area. However, compared with Mg(OH)2, Mg(OH)2RH increased osteoblastic differentiation by inducing ALP activity and promoting the expression of Runx2, SP7, Col1a1, and ALP, and had a negative effect on the expression of the osteoclastic genes NFATC1, CA2, and CTSK. These observations suggest the potential usefulness of Mg(OH)2RH NPs in bone regeneration.

scholarly journals MO564CHRONIC EXPOSURE TO INDOXYL SULFATE CHANGES BONE PROPERTIES AND EXPRESSION OF SIRT2, SIRT3, AND SIRT7 GENES*

2021 ◽  
Vol 36 (Supplement_1) ◽  
Author(s):  
Malgorzata Karbowska ◽  
Beata Sieklucka ◽  
Tomasz Domaniewski ◽  
Malgorzata Galazyn-Sidorczuk ◽  
Krystyna Pawlak ◽  
...  
Keyword(s):  
Bone Metabolism ◽  
Bone Tissue ◽  
Pure Water ◽  
Indoxyl Sulfate ◽  
Geometrical Parameters ◽  
Tartrate Resistant Acid Phosphatase ◽  
Uremic Toxin ◽  
Skeletal System ◽  
Flame Atomic Absorption ◽  
Bone Properties

Abstract Background and Aims Chronic kidney disease (CKD) is connected with accumulation of uremic toxins that leads to dysfunction of many systems including skeletal system. Indoxyl sulfate (IS) is one of the most potent protein-bound uremic toxin. However, its impact on bone and mineral disorders remains unclear. Sirtuins (SIRT) are members of the class III type nicotinamide adenine dinucleotide-dependent histone deacetylases. Their roles are commonly linked to aging, metabolism, and antioxidant defense. However, SIRT1, SIRT3 and SIRT7 maintain also bone formation. In turn, reduction of SIRT2 has a protective role due to reduced activation of osteoclasts-mediated bone resorption. Thus, the aim of this work was to evaluate IS impact on bone metabolism and bone properties, and SIRT genes expression. Method Male Wistar rats weighting 180 – 210 g were divided into 2 groups: experimental receiving IS in the dose of 200 mg/kg of b.w. for four weeks (200IS), and control receiving pure water (CON). After four weeks the femurs and tibiae were collected, and geometrical parameters (CI – cortical index, CSA – cortical sectional area, CSMI – cross sectional moment of inertia, MRWT – mean relative wall thickness) were measured. The IS bone (cortical and trabecular), plasma and urine levels were determined by high-performance liquid chromatography (HPLC). The concentrations of Ca, Mg, Zn, Fe, Mn, and Cu in bone tissue were determined by the flame atomic absorption spectrometry. Bone turnover markers (alkaline phosphatase – ALP, and tartrate-resistant acid phosphatase - TRAP) were determined in homogenates from trabecular and cortical left femurs. The expression of ALP, TRAP, SIRT1, SIRT2, SIRT3 and SIRT7 gene was assessed by QRT-PCR in right femurs. Results Plasma, urine, cortical and trabecular bone IS concentrations were increased in 200IS compared to CON. Geometrical properties of femur (CI, CSA, CSMI and MRWT) were significantly reduced in 200IS. There were no differences in relative femur weight and length between studied groups. The activity of ALP and TRAP in bone tissue were significantly lower in 200IS compared to CON. In turn, the expression of TRAP gene was increased in 200IS. There were no difference in concentrations of Ca, Mg, Fe, Mn, and Cu in bone tissue. Only Zn concentration in cortical bone was reduced in 200IS. The expressions of SIRT3 and SIRT7 were decreased in 200IS, whereas SIRT2 was increased in 200IS. Chronic exposure to IS did not affect expression of SIRT1. Conclusion Obtained results suggest that IS affects bone properties and bone metabolism, and has impact on expression of SIRT2, SIRT3, and SIRT7 genes. Our findings may help to better understand mechanisms leading to dysfunction of skeletal system during CKD. This work was supported by Polish National Science Centre (Grant No. 2017/27/N/NZ4/00033).

scholarly journals The Novel Role of PGC1α in Bone Metabolism

2021 ◽  
Vol 22 (9) ◽  
pp. 4670
Author(s):  
Cinzia Buccoliero ◽  
Manuela Dicarlo ◽  
Patrizia Pignataro ◽  
Francesco Gaccione ◽  
Silvia Colucci ◽  
...  
Keyword(s):  
Bone Metabolism ◽  
Osteoblastic Differentiation ◽  
In Vivo Studies ◽  
Peroxisome Proliferator ◽  
Sirtuin 3 ◽  
Peroxisome Proliferator Activated Receptor ◽  
Increased Risk

Peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC1α) is a protein that promotes transcription of numerous genes, particularly those responsible for the regulation of mitochondrial biogenesis. Evidence for a key role of PGC1α in bone metabolism is very recent. In vivo studies showed that PGC1α deletion negatively affects cortical thickness, trabecular organization and resistance to flexion, resulting in increased risk of fracture. Furthermore, in a mouse model of bone disease, PGC1α activation stimulates osteoblastic gene expression and inhibits atrogene transcription. PGC1α overexpression positively affects the activity of Sirtuin 3, a mitochondrial nicotinammide adenina dinucleotide (NAD)-dependent deacetylase, on osteoblastic differentiation. In vitro, PGC1α overexpression prevents the reduction of mitochondrial density, membrane potential and alkaline phosphatase activity caused by Sirtuin 3 knockdown in osteoblasts. Moreover, PGC1α influences the commitment of skeletal stem cells towards an osteogenic lineage, while negatively affects marrow adipose tissue accumulation. In this review, we will focus on recent findings about PGC1α action on bone metabolism, in vivo and in vitro, and in pathologies that cause bone loss, such as osteoporosis and type 2 diabetes.

Tartrate-resistant acid phosphatase activity and glutathione levels are modulated during hFOB 1.19 osteoblastic differentiation

2008 ◽  
Vol 39 (6) ◽  
pp. 627-634 ◽  
Author(s):  
Tatiana Salles de Souza Malaspina ◽  
Célio Xavier dos Santos ◽  
Ana Paula Campanelli ◽  
Francisco Rafael Martins Laurindo ◽  
Mari Cleide Sogayar ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Osteoblastic Differentiation ◽  
Tartrate Resistant Acid Phosphatase

Bone Metabolism, Bone Mineral Content, and Density in Elite Late Teen Female Sprinters

2021 ◽  
Author(s):  
Yuka Tsukahara ◽  
Suguru Torii ◽  
Fumihiro Yamasawa ◽  
Jun Iwamoto ◽  
Takanobu Otsuka ◽  
...  
Keyword(s):  
Bone Turnover ◽  
Bone Mineral Content ◽  
Bone Metabolism ◽  
Bone Mineral ◽  
Bone Turnover Markers ◽  
Mineral Content ◽  
Whole Body ◽  
Tartrate Resistant Acid Phosphatase ◽  
Intensive Training ◽  
Turnover Markers

AbstractWith intensive training, bone injuries are a major concern for athletes. To assess bone condition, we often measure bone turnover markers, bone mineral content and density; however, in junior athletes, it is not easy to distinguish changes caused by bone injuries from those caused by growth, because the metabolism is increased in both cases. Moreover, although some studies have examined female endurance athletes, knowledge regarding changes in static and dynamic bone conditions in late teen athletes is limited. In this study, we measured the bone mineral content and density, as well as bone turnover markers, in 40 elite female sprinters in their late teens. Whole body mode dual-energy X-ray absorptiometry was performed to measure bone mineral content and density. Blood samples were collected to determine bone resorption and formation markers at the end of track season in 2016 and during the same period of the following year. Body weight and bone mineral content significantly increased, and tartrate-resistant acid phosphatase type 5b, bone-type alkaline phosphatase, and osteocalcin significantly decreased after a year. Furthermore, the rate of change in bone mineral content was higher in younger athletes, indicating that bone growth approaches completion in the late teen years and that bone metabolism accordingly decreases.

Preparation of lamellar magnesium hydroxide nanoparticles via precipitation method

2009 ◽  
Vol 191 (3) ◽  
pp. 227-230 ◽  
Author(s):  
Wenjun Jiang ◽  
Xiao Hua ◽  
Qiaofeng Han ◽  
Xujie Yang ◽  
Lude Lu ◽  
...  
Keyword(s):  
Magnesium Hydroxide ◽  
Precipitation Method ◽  
Magnesium Hydroxide Nanoparticles

Influence of Angptl1 on Osteoclast Formation and Osteoblastic Phenotype in Mouse Cells.

2020 ◽  
Author(s):  
Masayoshi Ishida ◽  
Naoyuki Kawao ◽  
Yuya Mizukami ◽  
Yoshimasa Takafuji ◽  
Hiroshi Kaji
Keyword(s):  
Bone Remodeling ◽  
Adipogenic Differentiation ◽  
Mouse Cell ◽  
Osteoblastic Differentiation ◽  
Osteoclast Formation ◽  
Bone Morphogenetic Protein 2 ◽  
Raw 264.7 Cells ◽  
Tartrate Resistant Acid Phosphatase ◽  
Peroxisome Proliferator ◽  
Mrna Levels

Abstract Background: Osteoblasts and osteoclasts play important roles during the bone remodeling in the physiological and pathophysiological states. Although angiopoietin family Angiopoietin like proteins (Angptls), including Angptl1, have been reported to be involved in inflammation, lipid metabolism and angiogenesis, the roles of Angptl1 in bone have not been reported so far. Methods: We examined the effects of Angptl1 overexpression on the osteoblast and osteoclast phenotypes using mouse cell lines.Results: Angptl1 overexpression significantly inhibited the osteoclast formation and mRNA levels of tartrate-resistant acid phosphatase and cathepsin K enhanced by receptor activator of nuclear factor κB ligand in RAW 264.7 cells. Moreover, Angptl1 overexpression significantly enhanced Osterix mRNA levels, alkaline phosphatase activity and mineralization induced by bone morphogenetic protein-2 in ST2 cells, although it did not affect the expression of osteogenic genes in MC3T3-E1 cells. On the other hand, Angptl1 overexpression significantly reduced the mRNA levels of peroxisome proliferator-activated receptor γ and adipocyte protein-2 induced by adipogenic medium in 3T3-L1 cells.Conclusions: The present study first indicated that Angptl1 suppresses and enhances osteoclast formation and osteoblastic differentiation in mouse cell line, respectively, although it inhibits adipogenic differentiation of 3T3-L1 cells. These data suggest the possibility that Angptl1 might be physiologically related to bone remodeling.

Scaffold-supported extracellular matrices preserved by magnesium hydroxide nanoparticles for renal tissue regeneration

2020 ◽  
Vol 8 (19) ◽  
pp. 5427-5440 ◽  
Author(s):  
Yun Ah Kim ◽  
So Young Chun ◽  
Sung-Bin Park ◽  
Eunyoung Kang ◽  
Won-Gun Koh ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Tissue Regeneration ◽  
Magnesium Hydroxide ◽  
Renal Tissue ◽  
Extracellular Matrices ◽  
Magnesium Hydroxide Nanoparticles

Fibroblast-derived extracellular matrix-supported scaffolds made up of PLGA were prepared with the enhanced preservation of ECM components by composites with magnesium hydroxide nanoparticles, and were applied for renal tissue regeneration.

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