scholarly journals Enzymatic Hydrolysis of Poly(3-Hydroxybutyrate-co-3-Hydroxyvalerate) Scaffolds

Materials ◽  
2020 ◽  
Vol 13 (13) ◽  
pp. 2992 ◽  
Author(s):  
Adriana Kovalcik ◽  
Stanislav Obruca ◽  
Michal Kalina ◽  
Michal Machovsky ◽  
Vojtech Enev ◽  
...  
Keyword(s):  
Enzymatic Hydrolysis ◽  
Saline Solution ◽  
Morphological Changes ◽  
Carbon Sources ◽  
Grape Pomace ◽  
Degree Of Crystallinity ◽  
Lignocellulosic Waste ◽  
Hydrolysis Of ◽  
The Degree Of Crystallinity ◽  
By Products

Polyhydroxyalkanoates (PHAs) are hydrolyzable bio-polyesters. The possibility of utilizing lignocellulosic waste by-products and grape pomace as carbon sources for PHA biosynthesis was investigated. PHAs were biosynthesized by employing Cupriavidus necator grown on fructose (PHBV-1) or grape sugar extract (PHBV-2). Fifty grams of lyophilized grape sugar extract contained 19.2 g of glucose, 19.1 g of fructose, 2.7 g of pectin, 0.52 g of polyphenols, 0.51 g of flavonoids and 7.97 g of non-identified rest compounds. The grape sugar extract supported the higher production of biomass and modified the composition of PHBV-2. The biosynthesized PHAs served as matrices for the preparation of the scaffolds. The PHBV-2 scaffolds had about 44.2% lower crystallinity compared to the PHBV-1 scaffolds. The degree of crystallinity markedly influenced the mechanical behavior and enzymatic hydrolysis of the PHA scaffolds in the synthetic gastric juice and phosphate buffer saline solution with the lipase for 81 days. The higher proportion of amorphous moieties in PHBV-2 accelerated enzymatic hydrolysis. After 81-days of lasting enzymatic hydrolysis, the morphological changes of the PHBV-1 scaffolds were negligible compared to the visible destruction of the PHBV-2 scaffolds. These results indicated that the presence of pectin and phenolic moieties in PHBV may markedly change the semi-crystalline character of PHBV, as well as its mechanical properties and the course of abiotic or enzymatic hydrolysis.

scholarly journals Influence of Initial Alkalinity of Lignocellulosic Waste on Their Enzymatic Degradation

2014 ◽  
Vol 40 (2) ◽  
pp. 103-113
Author(s):  
Marcin Wołczyński ◽  
Marta Janosz-Rajczyk
Keyword(s):  
Enzymatic Hydrolysis ◽  
Enzymatic Degradation ◽  
Suspended Solids ◽  
Lignocellulosic Waste ◽  
Initial Exposure ◽  
Total Sugars ◽  
Physical Chemical ◽  
Effect Of Treatment ◽  
Hydrolysis Of ◽  
By Products

Abstract The presented results of research on the effectiveness of enzymatic hydrolysis of lignocellulosic waste, depending on their initial depolymerisation in alkaline medium were considered in the context of the possibility of their further use in the fermentation media focused on the recovery of energy in the form of molecular hydrogen. The aim of this study was to determine the appropriate dose and concentration of a chemical reagent, whose efficiency would be high enough to cause decomposition of the complex, but without an excessive production of by-products which could adversely affect the progress and effectiveness of the enzymatic hydrolysis and fermentation. The effect of treatment on physical-chemical changes of homogenates’ properties such as pH, COD, the concentration of monosaccharide and total sugars and the concentration of total suspended solids and volatile suspended solids was determined. The enzymatic decomposition of lignocellulosic complex was repeatedly more efficient if the sample homogenates were subjected to an initial exposure to NaOH. The degree of conversion of complex sugars into simple sugars during enzymatic hydrolysis of homogenates pre-alkalized to pH 11.5 and 12.0 was 83.3 and 84.2% respectively, which should be sufficient for efficient hydrogen fermentation process.

Study of structural and morphological changes during acсelerated oxidative degradation of mixtures of polyoxybutyrate with polycaprolactane

2021 ◽  
Vol 5 ◽  
pp. 60-69
Author(s):  
V. N. Vasilets ◽  
◽  
A. P. Pankina ◽  
E. A. Nemets ◽  
V. Yu. Belov ◽  
...  
Keyword(s):  
Morphological Changes ◽  
Oxidative Degradation ◽  
Initial Mixture ◽  
Polymer Composite Material ◽  
Degree Of Crystallinity ◽  
Fenton Reagent ◽  
Dsc Method ◽  
The Degree Of Crystallinity ◽  
Composite Samples ◽  
Scanning Electron

Structural and morphological changes in the films of poly(oxybirutyrate-co-oxyvalerate) (P(OB-OV)) with poly-e-caprolactone (PCL) were studied by IR spectroscopy, DSC, and scanning electron microscopy during accelerated oxidative degradation under tests in Fenton reagent. It is shown by the DSC method that the phase separation of the P(OB-OV) and PCL components is observed in the initial mixture. The melting of various phases of P(OB-OV) is observed at temperatures of 147 °C and 157 °C, and PCL melts at a temperature of 61 °C. The degree of crystallinity of P(OB-OV) and PCL in the mixture is 67 % and 50 %, respectively. It was found that the degradation of poly(oxybirutyrate-co-oxyvalerate) prevails during incubation of composite samples in Fenton solution for 2 to 12 weeks. By changing the ratio of the components, it is possible to significantly change the rate of oxidative degradation, the molecular weight and the degree of crystallinity of the polymer composite material P(OB-OV):PCL.

Effects of the Different Crystallization Time on Synthesis of Zeolite 4A from by-Products of Polysilicon Production Process

2011 ◽  
Vol 347-353 ◽  
pp. 246-251
Author(s):  
Xing Yong Liu ◽  
Min Li ◽  
Hong Chen ◽  
Wei Wei
Keyword(s):  
Production Process ◽  
Degree Of Crystallinity ◽  
Crystallization Time ◽  
Crystallinity Degree ◽  
Structure Morphology ◽  
Zeolite 4A ◽  
Single Structure ◽  
Ft Ir ◽  
The Degree Of Crystallinity ◽  
By Products

Pure form,single phase and high crystalline zeolite 4A samples were synthesized during hydrothermal treatment of by-products in polysilicon production process. The effects of the different crystallization time on the degree of crystallinity, skeleton structure, morphology, size of the particle and its distribution of zeolite 4A samples were investigated using XRD,FT-IR,SEM and Zetasizer. The results indicated that the zeolite 4A sample featured high crystallinity degree, excellent dispersivity, high purity and single structure.

Enzymatic Hydrolysis of Sardine (Sardina pilchardus) By-products and Lipid Recovery

2009 ◽  
Vol 18 (1-2) ◽  
pp. 120-134 ◽  
Author(s):  
Irineu Batista ◽  
Cristina Ramos ◽  
Rita Mendonça ◽  
Maria Leonor Nunes
Keyword(s):  
Enzymatic Hydrolysis ◽  
Sardina Pilchardus ◽  
Hydrolysis Of ◽  
By Products

Enzymatic hydrolysis of cod (Gadus morhua) by-products

2005 ◽  
Vol 40 (12) ◽  
pp. 3680-3692 ◽  
Author(s):  
Rasa Šližytė ◽  
Turid Rustad ◽  
Ivar Storrø
Keyword(s):  
Enzymatic Hydrolysis ◽  
Gadus Morhua ◽  
Hydrolysis Of ◽  
By Products

Enzymatic hydrolysis of fish protein by-products: Kinetic analysis

2008 ◽  
Vol 136 ◽  
pp. S378-S379
Author(s):  
S. Almonacid ◽  
C. Castillo ◽  
M. Pinto ◽  
A. Abakarov ◽  
R. Simpson
Keyword(s):  
Enzymatic Hydrolysis ◽  
Kinetic Analysis ◽  
Fish Protein ◽  
Hydrolysis Of ◽  
By Products

Effects of Enzymatic Hydrolysis Conditions on the Antioxidant Activity of Red Tilapia (Oreochromis spp.) Viscera Hydrolysates

2020 ◽  
Vol 21 (12) ◽  
pp. 1249-1258
Author(s):  
Cindy T. Sepúlveda ◽  
José E. Zapata
Keyword(s):  
Molecular Weight ◽  
Antioxidant Activity ◽  
Enzymatic Hydrolysis ◽  
Protein Concentration ◽  
Polynomial Equation ◽  
Degree Of Hydrolysis ◽  
Red Tilapia ◽  
Hydrolysis Conditions ◽  
Hydrolysis Of ◽  
By Products

Background: Fish is an essential source of nutrients for human nutrition due to the composition of proteins, vitamins, and minerals, among other nutrients. Enzymatic hydrolysis represents an alternative for the use of by-products of the aquaculture industry. Objective: We propose to evaluate the effect of stirring speed, temperature, and initial protein concentration on the degree of hydrolysis of proteins and antioxidant activity of red tilapia (Oreochromis spp.) viscera hydrolysates. Methods: The effect of stirring speed, temperature, and initial protein concentration on the degree of hydrolysis of proteins and antioxidant activity was evaluated using an experimental design that was adjusted to a polynomial equation. The hydrolysate was fractioned to determine the antioxidant activity of the fractions, and functional properties were also measured. Results: Stirring speed and protein concentration presented a statistically significant effect (p <0.05) on all the response variables. However, the temperature did not present a statistically significant effect on the degree of hydrolysis. Discussion: The best conditions of hydrolysis were stirring speed of 51.44 rpm, a temperature of 59.15°C, and the protein concentration of 10 g L-1. The solubility of the hydrolysate protein was high at different pH, and the hydrolysate fraction with the highest antioxidant activity has a molecular weight <1 kDa. Conclusion: The degree of hydrolysis and the biological activity of red tilapia viscera hydrolysates (Oreochromis spp.) are affected by temperature, substrate concentration, and stirring speed. The optimal conditions of hydrolysis allowed to obtain a hydrolysate with antioxidant activity are due to the peptides with low molecular weight.

Non-waste technology through the enzymatic hydrolysis of agro-industrial by-products

2018 ◽  
Vol 77 ◽  
pp. 64-76 ◽  
Author(s):  
Vitalijs Radenkovs ◽  
Karina Juhnevica-Radenkova ◽  
Paweł Górnaś ◽  
Dalija Seglina
Keyword(s):  
Enzymatic Hydrolysis ◽  
Hydrolysis Of ◽  
By Products

Synthesis of Single-Phase Zeolite 4A from by-Products of Polysilicon Production Process

2011 ◽  
Vol 396-398 ◽  
pp. 876-879 ◽  
Author(s):  
Xing Yong Liu ◽  
Min Li ◽  
Xi Yang He ◽  
Hong Chen ◽  
Shi Xiong Hao
Keyword(s):  
Production Process ◽  
Single Phase ◽  
Degree Of Crystallinity ◽  
Molar Ratio ◽  
Crystallization Time ◽  
Zeolite 4A ◽  
Influence Of Crystallization ◽  
Ft Ir ◽  
The Degree Of Crystallinity ◽  
By Products

In this paper, pure,single phase and high crystalline zeolite 4A samples were synthesized by hydrothermal treating by-products of polysilicon production process. The influence of crystallization time and the molar ratio of SiO2/Al2O3 on degree of crystallinity of synthesized zeolite 4A samples was studied. The degree of crystallinity, skeleton structure and particles morphology of zeolite 4A samples were characterized by XRD,FT-IR,SEM,respectively.The results show that synthesized zeolite 4A samples were identical with commodity zeolite 4A.

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