scholarly journals Effect of Cu- and Zn-Doped Bioactive Glasses on the In Vitro Bioactivity, Mechanical and Degradation Behavior of Biodegradable PDLLA Scaffolds

Materials ◽  
2020 ◽  
Vol 13 (13) ◽  
pp. 2908 ◽  
Author(s):  
Julian Bejarano ◽  
Aldo R. Boccaccini ◽  
Cristian Covarrubias ◽  
Humberto Palza
Keyword(s):  
Bioactive Glass ◽  
Bone Repair ◽  
Apatite Formation ◽  
High Porosity ◽  
Degradation Behavior ◽  
Polymer Scaffolds ◽  
Bioactive Glasses ◽  
Salt Leaching ◽  
Compression Properties

Biodegradable polymer scaffolds filled with bioactive glass particles doped with therapeutic metal ions are a novel and promising strategy to repair critical-sized bone defects. In this study, scaffolds based on a poly (D, L-lactide acid) (PDLLA) matrix filled with un-doped and Cu-, Zn- and CuZn-doped bioactive glass particles were produced by freeze-drying and a salt-leaching method. The effects of the doping and content of the glass particles (10 and 30 wt.%) on the morphology, compression properties, apatite formation, and degradation behavior of the scaffolds were evaluated. The scaffolds presented high porosity (~93%) with pores ranged from 100 to 400 μm interconnected by smaller pores and this porosity was kept after the glass particles incorporation. The glass particles reinforced the polymer scaffolds with improvements as high as 130% in elastic moduli, and further promoted the apatite formation on the scaffold surface, both properties depending on the amount and type of filler. The bioactive glass particles boosted the scaffold degradation with the PDLLA/un-doped glass scaffold showing the highest rate, but still retaining structural and dimensional integrity. Our findings show that the incorporation of un-doped and metal-doped bioactive glasses increases the mechanical strength, promotes the bioactivity and modifies the degradation profile of the resulting polymer/glass scaffolds, making them better candidates for bone repair.

scholarly journals Strontium-substituted sub-micron bioactive glasses inhibit ostoclastogenesis through suppression of RANKL-induced signaling pathway

2020 ◽  
Vol 7 (3) ◽  
pp. 303-311 ◽  
Author(s):  
Deqiu Huang ◽  
Fujian Zhao ◽  
Wendong Gao ◽  
Xiaofeng Chen ◽  
Zhouyi Guo ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Signaling Pathway ◽  
Bone Repair ◽  
Superior Performance ◽  
Bioactive Glasses ◽  
Repair Materials ◽  
Potential Impact ◽  
Bone Repair Materials

Abstract Strontium-substituted bioactive glass (Sr-BG) has shown superior performance in bone regeneration. Sr-BG-induced osteogenesis has been extensively studied; however, Sr-BG-mediated osteoclastogenesis and the underlying molecular mechanism remain unclear. It is recognized that the balance of osteogenesis and osteoclastogenesis is closely related to bone repair, and the receptor activators of nuclear factor kappaB ligand (RANKL) signaling pathway plays a key role of in the regulation of osteoclastogenesis. Herein, we studied the potential impact and underling mechanism of strontium-substituted sub-micron bioactive glass (Sr-SBG) on RANKL-induced osteoclast activation and differentiation in vitro. As expected, Sr-SBG inhibited RANKL-mediated osteoclastogenesis significantly with the experimental performance of decreased mature osteoclasts formation and downregulation of osteoclastogenesis-related gene expression. Furthermore, it was found that Sr-SBG might suppress osteoclastogenesis by the combined effect of strontium and silicon released through inhibition of RANKL-induced activation of p38 and NF-κB pathway. These results elaborated the effect of Sr-SBG-based materials on osteoclastogenesis through RANKL-induced downstream pathway and might represent a significant guidance for designing better bone repair materials.

scholarly journals The Influence of Na and Ti on theIn VitroDegradation and Bioactivity in 58S Sol-Gel Bioactive Glass

2012 ◽  
Vol 2012 ◽  
pp. 1-7 ◽  
Author(s):  
Shirong Ni ◽  
Ruilin Du ◽  
Siyu Ni
Keyword(s):  
Activation Energy ◽  
Bioactive Glass ◽  
Body Fluid ◽  
Sol Gel ◽  
Apatite Formation ◽  
Ion Release ◽  
Buffer Solution ◽  
Bioactive Glasses ◽  
Initial Stage

The aim of this study was to investigate the effect of Na and Ti on thein vitrodegradation and bioactivity in the 58S bioactive glass. The degradation was evaluated through the activation energy of Si ion release from bioactive glasses and the weight loss of bioactive glasses in Tris-HCl buffer solution. Thein vitrobioactivity of the bioactive glasses was investigated by analysis of apatite-formation ability in the simulated body fluid (SBF). The results showed that Na in the 58S glass accelerated the dissolution rate of the glass, whereas Ti in the 58S glass slowed down the rate of glass solubility. Bioactivity tests showed that Na in glass increased the apatite-forming ability in SBF. In contrast, Ti in glass retards the apatite formation at the initial stage of SBF soaking but does not affect the growth of apatite after long periods of soaking.

Material Properties of Strontium Doped Bioactive Glasses/Hydroxyapatite Composite and Its Mechanism of Promoting Bone Repair

2021 ◽  
Vol 11 (12) ◽  
pp. 2313-2320
Author(s):  
Jian Zhao ◽  
Wei Li ◽  
Xin Dong ◽  
Jiying Chen
Keyword(s):  
Bone Repair ◽  
Sol Gel ◽  
Precipitation Method ◽  
Dissolution Behavior ◽  
Immersion Method ◽  
Bioactive Glasses ◽  
Strontium Content ◽  
M2 Polarization ◽  
Good Ability

Based on bioactive glasses (BG) of 58S, sol–gel method is used to prepare strontium oxide substituted bioactive glasses (SrO-BG) with different strontium content. SrO-BG and nano hydroxyapatite (HAp) composite materials were synthesized using precipitation method. The phase composition and morphologies of the prepared materials were examined by x-ray diffraction (XRD) and scanning electron microscopy (SEM) techniques. The dissolution and bio-mineralization of SrO-BG and SrO-BG/HAp composites in SBF are investigated by immersion method. The effects of secretion components of macrophages regulated by strontium doped SrO-BG/HAp composites on the osteogenic differentiation (OD) of bone marrow mesenchymal stem cells (BMSCs) are analyzed. The results demonstrate that the SrO-BG can inhibit the dissolution of BG. Different proportions of SrO-BG/HAp composites show good ability to induce HAp in SBF. The bio-mineralization ability of SrO-BG/HAp composites increases with the increase of SrO-BG content. The results of dissolution behavior and bio-mineralization of SrO-BG/HAp composite show that the dissolution rate of each ion can be controlled by adjusting the content of SrO-BG in the composite, and then the degradation rate can effectively be controlled. The results of in vitro experiments show that SrO-BG/HAp composites with 2%, 5% and 8% strontium content are more effective in promoting M2 polarization of macrophages than SrO-BG/HAp composites with 0% strontium content. Among them, 5% strontium doped SrO-BG/HAp has the strongest effect on M2 polarization of macrophages, and the secretion of macrophages regulated by 5% strontium doped SrO-BG/HAp composite is more conducive to bone repair.

Preparation and In Vitro Characterization of Polycaprolactone and Demineralized Bone Matrix Scaffolds

2012 ◽  
Vol 1417 ◽  
Author(s):  
Titilayo Moloye ◽  
Christopher Batich
Keyword(s):  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Apatite Formation ◽  
Alizarin Red ◽  
Salt Leaching ◽  
Synthetic Materials ◽  
Calcium And Phosphorus ◽  
Demineralized Bone

ABSTRACTCylindrical porous polycaprolactone (PCL) scaffolds containing 25, 35, and 50 wt% demineralized bone matrix (DBM) were fabricated using a salt-leaching method for application in bone engineering. In the present work, PCL-DBM scaffolds were monitored for calcium and phosphorus deposition in both deionized (DI) water and simulated body fluid (SBF) for time periods of 5, 10, 15, and 20 days at 37°C under constant rotation. An in vitro assessment of the bioactivity of synthetic materials using SBF under physiological conditions can be used as a barometer of scaffold behavior in vivo. DBM, an osteoinductive material, was used to gauge if there was a correlation between the concentration of DBM within a scaffold and the apatite formation on its surface. Biochemical assays, alizarin red S staining, and scanning electron microscopy (SEM) with elemental analysis of calcium and phosphorus were consistent in that they confirmed that PCL scaffolds containing 35 wt% DBM in SBF at 14 days post-immersion showed signs of early apatite formation.

scholarly journals Hierarchical Structures and Shaped Particles of Bioactive Glass and ItsIn VitroBioactivity

2013 ◽  
Vol 2013 ◽  
pp. 1-6 ◽  
Author(s):  
U. Boonyang ◽  
F. Li ◽  
A. Stein
Keyword(s):  
Body Temperature ◽  
Simulated Body Fluid ◽  
Bioactive Glass ◽  
Body Fluid ◽  
Colloidal Crystals ◽  
Hierarchical Structures ◽  
The Other ◽  
Bioactive Glasses ◽  
Ordered Macroporous

In this study, bioactive glass particles with controllable structure and porosity were prepared using dual-templating methods. Block copolymers used as one template component produced mesopores in the calcined samples. Polymer colloidal crystals as the other template component yielded either three-dimensionally ordered macroporous (3DOM) products or shaped bioactive glass nanoparticles. Thein vitrobioactivity of these bioactive glasses was studied by soaking the samples in simulated body fluid (SBF) at body temperature (37°C) for varying lengths of time and monitoring the formation of bone-like apatite on the surface of the bioactive glass. A considerable bioactivity was found that all of bioactive glass samples have the ability to induce the formation of an apatite layer on its surface when in contact with SBF. The development of bone-like apatite is faster for 3DOM bioactive glasses than for nanoparticles.

scholarly journals The Evaluation of Formation and Bioactivity of New Sol-gel Bioactive Glass

2019 ◽  
Vol 35 (1) ◽  
Author(s):  
Bui Xuan Vuong
Keyword(s):  
Bioactive Glass ◽  
Sol Gel ◽  
Glass Ceramics ◽  
Medical Applications ◽  
Crystalline Solids ◽  
Bioactive Glasses ◽  
Central European Journal ◽  
Central European

In this paper, three ceramic compositions 50SiO2-50CaO (A), 45SiO2-45CaO-10P2O5 (B) and 40SiO2-40CaO-20P2O5 (C) (wt %) were synthesized by using the sol-gel technique. XRD analysis demonstrates that only sample C can form the glass material. Treated temperatures and heated times were also evaluated. Analysis data showed that the bioglass 40SiO2-40CaO-20P2O5 (wt %) can successfully elaborate when the ceramic powder heated at 750 oC for 3 hours. ‘‘In vitro’’ experiment was effectuated to investigate the bioactivity of bioglass 40SiO2-40CaO-20P2O5 by soaking powder samples in SBF solution. Obtained result confirmed the formation of hydroxyapatite (HA) phase on glass’s surface after 15 days of immersion, in which HA formation orients following (211) and (222) miller planes in crystalline structure of HA phase. Keywords Sol-gel; bioglass; hydroxyapatite; SBF; bioactivity References [1] D.F. Williams, Definitions in Biomaterials, Consensus Conference for the European Society for Biomaterials, Chester, UK, 1986.[2] L.L. Hench, Bioceramics: From Concept to Clinic, Journal of the American Ceramic Society, 74 (1991) 1487.[3] L.L. Hench, The story of Bioglass, Journal of Materials Science: Materials in Medicine, 17 (2006) 967.[4] X.V. Bui, H. Oudadesse, Y. Le Gal, A. Mostafa, P.Pellen and G. Cathelineau, Chemical Reactivity of Biocomposite Glass-Zoledronate, Journal of the Australian Ceramic Society, 46 (2010) 24.[5] L.L. Hench, Genetic design of bioactive glass, Journal of the European Ceramic Society, 29 (2009) 1257.[6] S. Kumar, P. Vinatier, A. Levasseur, K.J. Rao, Investigations of structure and transport in lithium and silver borophosphate glasses, Journal of Solid State Chemistry, 177 (2004)1723.[7] Z. Hong, A. Liu, L. Chen, X. Chen, X. Jing, Preparation of bioactive glass ceramic nanoparticles by combination of sol–gel and coprecipitation method, Journal of Non-Crystalline Solids, 355 (2009) 368.[8] D.B. Joroch, D.C. Clupper, Modulation of zinc release from bioactive sol–gel derived SiO2‐CaO‐ZnO glasses and ceramics, Journal of Biomedical Materials Research Part A, 82A (2007) 575.[9] J. Roman, S. Padilla, M. Vallet-Regi, Sol−Gel Glasses as Precursors of Bioactive Glass Ceramics, Chemistry of Materials, 15 (2003) 798.[10] J. Lao, J.M. Nedelec, Ph. Moretto, E. Jallot, Biological activity of a SiO2-CaO-P2O5 sol-gel glass highlighted by PIXE-RBS methods, Nuclear Instruments and Methods in Physics Research Section B, 245 (2006) 511.[11] [11] M. Vallet-Regi, L. Ruiz-Gonzalez, I. Izquierdo, J.M. Gonzalez-Calbet, Revisiting silica based ordered mesoporous materials: medical applications, Journal of Materials Chemistry, 16 (2006) 26.[12] W. Xia, J. Chang, Preparation and characterization of nano-bioactive-glasses (NBG) by a quick alkali-mediated sol–gel method, Materials Letters 61 (2007) 3251.[13] R. Li, A.E. Clark, L.L. Hench, An investigation of Bioactive Glass Powders by Sol-Gel Processing, Transactions of 16th Annual Meeting of the Societey for Biomaterials, 12 (1990) 40.[14] J. Lao, J.M. Nedelec, P. Moretto, E. Jallot, Imaging physicochemical reactions occurring at the pore surface in binary bioactive glass foams by micro ion beam analysis, Applied Materials and Interfaces, 6 (2010) 1737.[15] A. Balamurugan, G. Balossier, S. Kannan, J. Michel, A.H.S. Rebelo, J.M.F. Ferreira, Development and in vitro characterization of sol–gel derived CaO–P2O5–SiO2–ZnO bioglas, Acta Biomaterialia, 3 (2007) 255.[16] Z. Hong, A. Liu, L. Chen, X. Chen, X. Jing, Bioactive glass prepared by sol–gel emulsion, Journal of Non-Crystalline Solids, 355 (2009) 368.[17] O. Peital, E.D. Zanotto, L.L. Hench, Highly bioactive P2O5-Na2O-CaO-SiO2 glass-ceramics, Journal of Non-Crystalline Solids, 292 (2001) 115.[18] J. Liu, X. Miao, Sol-gel derived bioglass as a coating material for porous alumina scaffolds, Ceramics International, 30 (2004) 1781.[19] T. Kokubo, H. Takadama, How useful is SBF in predicting in vivo bone bioactivity. Biomaterials 27 (2006) 2907.[20] M. Dziadek, B. Zagrajczuk, P. Jelen, Z. Olejniczak, K.C. Kowalska, Structural variations of bioactive glasses obtained by different synthesis routes, Ceramics International, 42 (2016) 14700.[21] R. Lakshmi, V. Velmurugan and S. Sasikumar, Preparation and Phase Evolution of Wollastonite by Sol-Gel Combustion Method Using Sucrose as the Fuel, Combustion Science and Technology, 185 (2013) 1777.[22] G. Voicu, A. Bădănoiu, E. Andronescu1, C. M. Chifiruc, Synthesis, characterization and bioevaluation of partially stabilized cements for medical applications, Central European Journal of Chemistry, 11 (2013) 1657.[23] M.V. Regi, Ceramics for medical applications, Journal of the Chemical Society, Dalton Transactions, 2 (2001) 97.[24] G. Voicu, A.I. Bădănoiu, E. Andronescu, C.M. Chifiruc, Synthesis, characterization and bioevaluation of partially stabilized cements for medical applications, Central European Journal of Chemistry, 11 (2013) 1657.M. Wu, T. Wang, Y. Wang, F. Li, M. Zhou, X. Wu, A novel and facile route for synthesis of fine tricalcium silicate powders, Materials letters, 227 (2018), 187.

scholarly journals Effects of Additional Use of Bioactive Glasses or a Hydroxyapatite Toothpaste on Remineralization of Artificial Lesions in vitro

2020 ◽  
Vol 54 (4) ◽  
pp. 336-342
Author(s):  
Philipp Körner ◽  
Jana A Schleich ◽  
Daniel B. Wiedemeier ◽  
Thomas Attin ◽  
Florian J. Wegehaupt
Keyword(s):  
Bioactive Glass ◽  
Artificial Saliva ◽  
Test Group ◽  
In Vitro Study ◽  
Negative Control Group ◽  
Negative Control ◽  
Control Group ◽  
Bioactive Glasses ◽  
Initial Caries

<b><i>Objectives:</i></b> This in vitro study aimed to evaluate and compare the effect of two different bioactive glasses, a hydroxyapatite-containing, fluoride-free toothpaste (HTP) and a fluoride toothpaste (FTP) on the remineralization behavior of initial caries lesions. <b><i>Materials and Methods:</i></b> A total of 100 bovine enamel samples were randomly allocated to five groups of 20 samples each: NC = negative control group (artificial saliva); HTP = HTP group (Karex); FTP = FTP group (Elmex caries protection, 1,400 ppm); FTP + BG<sub>nano</sub> = FTP followed by Actimins bioactive glass; FTP + BG<sub>amorph</sub> = FTP followed by Schott bioactive glass. Radiographic documentation (advanced transversal microradiography; aTMR) was applied before and after all samples were exposed to a demineralizing gel for 10 days. Over a period of 28 days, samples were covered twice a day (every 12 h) with a toothpaste slurry of the respective test group or with artificial saliva in NC for 60 s and brushed with 15 brushing strokes. Samples in FTP + BG<sub>nano</sub> and FTP + BG<sub>amorph</sub> were additionally treated with the respective bioactive glass slurry for 30 s after brushing with the FTP. In the meantime, all samples were stored in artificial saliva. After 28 days, the structure of all samples was assessed again using aTMR and compared to the values measured after demineralization. The statistical evaluation of the integrated mineral loss was performed using Kruskal-Wallis test followed by a post hoc Conover test. <b><i>Results:</i></b> The FTP revealed the significantly highest increase of mineral content while the HTP showed the significantly lowest remineralization. Compared to artificial saliva, the use of the HTP or the combined application of FTP followed by bioactive glasses (FTP + BG<sub>nano</sub> and FTP + BG<sub>amorph</sub>) showed no significant remineralization. <b><i>Conclusion:</i></b> Under remineralizing in vitro conditions, brushing with 1,400 ppm FTP induced significantly more remineralization compared to storage in artificial saliva. The additional administration of both bioactive glasses as well as the substitutional brushing with an HTP resulted in significantly less remineralization compared to brushing with 1,400 ppm FTP.

scholarly journals Bioactive Glass and Silicate-Based Ceramic Coatings on Metallic Implants: Open Challenge or Outdated Topic?

Materials ◽  
2019 ◽  
Vol 12 (18) ◽  
pp. 2929 ◽  
Author(s):  
Giulia Brunello ◽  
Hamada Elsayed ◽  
Lisa Biasetto
Keyword(s):  
Ceramic Coatings ◽  
Apatite Formation ◽  
Bioactive Glasses ◽  
Coating Materials ◽  
Bioactive Coatings ◽  
Metallic Implants ◽  
Speed Up

The overall success and long-term life of the medical implants are decisively based on the convenient osseointegration at the hosting tissue-implant interface. Therefore, various surface modifications and different coating approaches have been utilized to the implants to enhance the bone formation and speed up the interaction with the surrounding hosting tissues, thereby enabling the successful fixation of implants. In this review, we will briefly present the main metallic implants and discuss their biocompatibility and osseointegration ability depending on their chemical and mechanical properties. In addition, as the main goal of this review, we explore the main properties of bioactive glasses and silica-based ceramics that are used as coating materials for both orthopedic and dental implants. The current review provides an overview of these bioactive coatings, with a particular emphasis on deposition methods, coating adhesion to the substrates and apatite formation ability tested by immersion in Simulated Body Fluid (SBF). In vitro and in vivo performances in terms of biocompatibility, biodegradability and improved osseointegration are examined as well.

In vitro degradation behavior of a hydroxyapatite/poly(lactide-co-glycolide) composite reinforced by micro/nano-hybrid poly(glycolide) fibers for bone repair

2017 ◽  
Vol 5 (44) ◽  
pp. 8695-8706 ◽  
Author(s):  
Yuhang Zhu ◽  
Zongliang Wang ◽  
Linlong Li ◽  
Daqian Gao ◽  
Qinli Xu ◽  
...  
Keyword(s):  
Mechanical Strength ◽  
Bone Repair ◽  
Degradation Behavior ◽  
Fiber Reinforced ◽  
In Vitro Degradation ◽  
High Mechanical Strength

A poly(glycolide) (PGA) fiber-reinforced hydroxyapatite/poly(lactide-co-glycolide) (HA/PLGA) composite with high mechanical strength has been prepared previously.

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