scholarly journals Cytotoxicity of Self-Etch Versus Etch-and-Rinse Dentin Adhesives: A Screening Study

Materials ◽  
2020 ◽  
Vol 13 (2) ◽  
pp. 452
Author(s):  
Luisa Fröb ◽  
Stefan Rüttermann ◽  
Georgios E. Romanos ◽  
Eva Herrmann ◽  
Susanne Gerhardt-Szép
Keyword(s):  
Quantitative Evaluation ◽  
Human Fibroblasts ◽  
Qualitative Assessment ◽  
Test Material ◽  
Reactivity Index ◽  
Dentin Adhesives ◽  
Etch And Rinse ◽  
Significant Difference ◽  
Self Etch

Six dentin adhesives were tested in vitro regarding their cytotoxicity on human fibroblasts. The adhesives Hybrid Bond, One-up Bond F Plus, AdheSE, Clearfil SE Bond, Optibond Solo Plus and Syntac were eluted with culture medium as single or sequentially applied adhesive part for 24 h. 75 Petri dishes were produced per group. They were evaluated triangulated, comprising the quantitative evaluation (105 ones) to determine “viable”, “dead” and “debris” cells with the use of a cell-counter and the reactivity index was also identified based on the qualitative assessment (420 ones). One-up Bond F Plus, AdheSE and Clearfil SE Bond showed a statistical difference of viable cells to the cell control. For One-up Bond F Plus, statistically, differences compared to hybrid bond and Syntac were also found. All the adhesives except One-up Bond F Plus showed significant differences between single and sequentially applied adhesive part regarding the quantitative evaluation. The test material showed a moderate grade of cytotoxicity. As a result, a statistically significant difference of the cytotoxicity between the self-etch and etch-and-rinse adhesives cannot be demonstrated regarding the qualitative evaluation and the reactivity index, but the differences between sequentially applied and single applied components can be proved.

scholarly journals Microshear Bond Strength of Different Adhesive Systems to Dentin

2019 ◽  
Author(s):  
Sara Valizadeh ◽  
Aida Moradi ◽  
Mansooreh Mirazei ◽  
Hooman Amiri ◽  
Mohammad Javad Kharazifard
Keyword(s):  
Bond Strength ◽  
Testing Machine ◽  
Single Bond ◽  
Adhesive Systems ◽  
Etch And Rinse ◽  
Significant Difference ◽  
Microshear Bond Strength ◽  
Self Etch ◽  
Composite Cylinders

Objectives: The aim of this study was to compare the microshear bond strength (µSBS) of various adhesive systems to dentin. Materials and Methods: In this in vitro experimental study, 60 sound human third molars were divided into four groups. Dentin discs were prepared of middle-third dentin measuring 4 mm in diameter and 2 mm in thickness. Dentin surfaces were bonded with one of the four types of adhesives: (A) Single Bond (3M ESPE), Scotchbond Universal (3M ESPE) in etch and rinse (B) and self-etch (C) modes and (D) Clearfil SE Bond (Kuraray Noritake Dental). After the application of adhesive systems according to the manufacturers’ instructions, composite cylinders (Vit-l-escence) were bonded to dentin surfaces. The μSBS test was performed using a universal testing machine at a crosshead speed of 1 mm/min. Data were analyzed with one-way ANOVA and Tukey’s test (α=0.05). Results: The µSBS was the highest in self-etch Scotchbond Universal (15.8±6.08 MPa) followed by Clearfil SE Bond (15.24±4.6 MPa), etch and rinse Scotchbond Universal (11.68±4.07MPa) and Single Bond (11.24±3.74 MPa). A significant difference was only found between Single Bond and etch and rinse Scotchbond Universal groups (P=0.04). Conclusion: Based on the results of this study, application of Scotchbond Universal in self-etch mode provides a reliable bond to dentin.

scholarly journals Microshear Bond Strength of Different Adhesive Systems to Dentin

2019 ◽  
Author(s):  
Sara Valizadeh ◽  
Aida Moradi ◽  
Mansooreh Mirazei ◽  
Hooman Amiri ◽  
Mohammad Javad Kharazifard
Keyword(s):  
Bond Strength ◽  
Testing Machine ◽  
Single Bond ◽  
Adhesive Systems ◽  
Etch And Rinse ◽  
Significant Difference ◽  
Microshear Bond Strength ◽  
Self Etch ◽  
Composite Cylinders

Objectives: The aim of this study was to compare the microshear bond strength (µSBS) of various adhesive systems to dentin. Materials and Methods: In this in vitro experimental study, 60 sound human third molars were divided into four groups. Dentin discs were prepared of middle-third dentin measuring 4 mm in diameter and 2 mm in thickness. Dentin surfaces were bonded with one of the four types of adhesives: (A) Single Bond (3M ESPE), Scotchbond Universal (3M ESPE) in etch and rinse (B) and self-etch (C) modes and (D) Clearfil SE Bond (Kuraray Noritake Dental). After the application of adhesive systems according to the manufacturers’ instructions, composite cylinders (Vit-l-escence) were bonded to dentin surfaces. The μSBS test was performed using a universal testing machine at a crosshead speed of 1 mm/min. Data were analyzed with one-way ANOVA and Tukey’s test (α=0.05). Results: The µSBS was the highest in self-etch Scotchbond Universal (15.8±6.08 MPa) followed by Clearfil SE Bond (15.24±4.6 MPa), etch and rinse Scotchbond Universal (11.68±4.07MPa) and Single Bond (11.24±3.74 MPa). A significant difference was only found between Single Bond and etch and rinse Scotchbond Universal groups (P=0.04). Conclusion: Based on the results of this study, application of Scotchbond Universal in self-etch mode provides a reliable bond to dentin.

Evaluation of the Bond Strength of Different Pulp Capping Materials to Dental Adhesive Systems: An In Vitro Study

2021 ◽  
pp. 232020682199798
Author(s):  
Sema Yazici Akbiyik ◽  
Elif Pınar Bakir ◽  
S¸eyhmus Bakir
Keyword(s):  
Bond Strength ◽  
In Vitro Study ◽  
Adhesive System ◽  
Adhesive Systems ◽  
Pulp Capping ◽  
Etch And Rinse ◽  
Significant Difference ◽  
Fracture Types ◽  
Self Etch

Aim: To evaluate the bond strengths of pulp capping materials (Dycal, ProRoot MTA, Biodentine, TheraCal LC, Calcimol LC, and ApaCal ART) and different adhesive systems (Gluma 2 Bond, Clearfil SE Protect, Gluma Self Etch, Clearfil S 3 Bond Plus, Gluma Bond Universal, Clearfil S 3 Bond Universal). Materials and Methods: Two hundred fifty-two acrylic blocks were prepared in which cylindrical cavities of 4 × 2 mm 3 were formed. Pulp capping materials were placed in the cavities. Different adhesive systems were applied to each pulp capping material group. After applying the composite resin, the shear bond strength (SBS) values of the specimens were determined in the Instron test device. Fracture types were evaluated using a stereomicroscope and a scanning electron microscope. Data were analyzed by Shapiro–Wilk’s and Kruskal–Wallis H test. Results: There is a statistically significant difference between pulp capping materials in terms of SBS values ( P < .05). Dycal’s SBS was found significantly lower than other materials, and the highest bond strength was observed in Calcimol LC material. Although there is no statistically significant difference ( P > .05) between the adhesive agent groups in terms of SBS, Gluma 2 Bond showed the highest bond strength value. Conclusion: In traditional pulp capping materials such as Dycal, MTA, and Biodentine, using a two-step self-etch adhesive system can result in higher bond strength values. In resin-based TheraCal LC,, ApaCal ART, and Calcimol LC materials, it may be recommended to use a two-step etch and rinse adhesive system.

Ultramorphological Characterization of the Resin Dentin Interface – An in vitro Analysis of Nanoleakage Patterns of Dentin Adhesives

2009 ◽  
Vol 33 (3) ◽  
pp. 223-230 ◽  
Author(s):  
Prasanna Neelakantan ◽  
Kavitha Sanjeev ◽  
CV Subba Rao
Keyword(s):  
Adhesive System ◽  
Hybrid Layer ◽  
Bonding Agents ◽  
Dentin Adhesives ◽  
Etch And Rinse ◽  
High Bond ◽  
Vitro Analysis ◽  
Self Etch

Aim and Design: The requirements of an effective dentin adhesive system include the ability to thoroughly infiltrate the collagen network and partially demineralized zone, to encapsulate the collagen and hydroxyapatite crystallites, to produce a well polymerized durable hybrid layer with high bond strengths. Microleakage and marginal percolation are the most detrimental factors thwarting the success of any restoration . However, the presence of leakage pathways, called nanoleakage have been observed in the hybrid layer,even in the absence of microleakage. The nanoleakage patterns of four dentin adhesives (Prime & Bond NT,AdheSE, Clearfil S3 bond and Fuji Bond LC) was compared using Scanning electron microscopy and Energy Dispersive Spectroscopy, by the silver nitrate penetration method. Results and conclusion: The results indicated that etch and rinse adhesives showed the maximum nanoleakage followed by the self etch systems. The glass ionomer based bonding agents showed the least nanoleakage. It is extremely important to take nanoleakage as a consideration while choosing dentin adhesives, to ensure success of the restoration.

scholarly journals Effect of an erosive challenge on the enamel-composite resin interface in primary teeth: an in vitro study

2015 ◽  
Vol 3 (3) ◽  
pp. 688
Author(s):  
Jonas Almeida Rodrigues ◽  
Cristiane Meira Assunção ◽  
Rogério Nanini Macanhão ◽  
Leila Canarin Vieira ◽  
Angela Scarparo
Keyword(s):  
Primary Teeth ◽  
Composite Resin ◽  
In Vitro Study ◽  
Adhesive Systems ◽  
Incomplete Removal ◽  
Etch And Rinse ◽  
Significant Difference ◽  
Composite Interface ◽  
Self Etch

AIM: The aim of this study was to evaluate whether different adhesive systems (etch-and-rinse or self-etch) render enamel-composite resin interface in primary teeth more susceptible to erosive challenge. MATERIAL AND METHODS: Thirty enamel specimens from caries-free primary incisors were selected and cavities were prepared for restoration. According to adhesive protocols, specimens were divided into groups: G1 (Adper Single Bond 2), G2 (Adper SE Plus), and G3 (35% phosphoric acid + Adper SE Plus). After restorative procedures, half of the surface of enamel and restorative material was protected with nail varnish, thus, only half of the sample was subjected to the erosive challenge (immersion in Coca-Cola®, 3 cycles of 5 minutes, for 5 days). Samples were analysed quantitatively through Knoop microhardness, the indentations were made on enamel-composite interface. Data were submitted to statistical analysis (Student’s t test, two-way ANOVA, p<0.05). RESULTS: It showed that different adhesive systems did not significantly affect the percentage of superficial microhardness change after an erosive challenge (p=0.387). However, although no significant difference was observed, G2 (self-etch system) showed the lowest percentage of superficial microhardness change. CONCLUSION: The use of different adhesive systems did not influence superficial microhardness of enamel-composite interface after an erosive challenge. The incomplete removal of the smear layer, though self-etch systems, suggests a greater ability to withstand the erosive challenge on the enamel-resin interface.

Two Adhesive Systems-effect on Adhesion to Tooth Structure

2019 ◽  
Vol 70 (7) ◽  
pp. 2608-2613
Author(s):  
Larisa Simona Deac ◽  
Kamel Earar ◽  
Adela Loredana Colceriu Burtea ◽  
Alexandra Stefania Berghe ◽  
Aurora Antoniac ◽  
...  
Keyword(s):  
Adhesive Systems ◽  
Hybrid Layer ◽  
Tooth Structure ◽  
Selective Etch ◽  
Etch And Rinse ◽  
Significant Difference ◽  
Wisdom Teeth ◽  
Group 2 ◽  
Self Etch ◽  
Group 1

This study evaluates and compares by dye penetration method and SEM photomicrographs the sealing obtained using two different classes of adhesive systems (etch-and-rinse and self-etch with selective etching) with SDR (Dentsply) bulk fill composite. 84 class V cavities were prepared on oral and vestibular face of 42 intact, freshly extracted wisdom teeth. The cavities were randomly divided in two groups and restored: Group 1 with prime &bond one select (Dentsply) and SDR (Dentsply) and Group 2 with prime&bond one Etch&Rinse (Dentsply) and SDR (Dentsply). Prime&bond one Select (Dentsply) is a single component adhesive and can be used in self etch mode, in selective enamel etch mode, or in etch-and-rinse mode. We chosen for this study the selective etch of the enamel mode. Prime&bond one Etch Rinse (Dentsply) is a universal etch-and-rinse one-bottle dental adhesive, designed to be used in two steps. The bulk fill composites are commonly used in modern dentistry due to their properties of low polymerization shrinkage and curing in layer of 4 mm depth, offering the practitioner a fast clinical procedure with good results. The results showed a good sealing at enamel and dentin margins with no statistically significant difference between adhesives, even though the mean of enamel infiltration was smaller for Group1. Furthermore the results show that there were differences between the two groups, for the infiltrations at the enamel, the values of microleakage being arithmetically higher for Group 1, but with no statistically difference between the two groups.SEM images showed for both groups a good adhesion surface with the tooth, but the hybrid layer of the total-etch adhesives is different from the hybrid layer formed by self etch adhesives, in terms of thickness, uniformity. In conclusion both adhesive systems have equivalent sealing qualities and can be successfully used with SDR.

scholarly journals Laboratory and clinical trials of cocamide diethanolamine lotion against head lice

PeerJ ◽  
2015 ◽  
Vol 3 ◽  
pp. e1368 ◽  
Author(s):  
Ian F. Burgess ◽  
Elizabeth R. Brunton ◽  
Christine M. Brown
Keyword(s):  
Clinical Study ◽  
Laboratory Tests ◽  
Head Louse ◽  
Test Material ◽  
Single Application ◽  
Louse Infestation ◽  
Treatment Regimens ◽  
Significant Difference ◽  
Head Louse Infestation

Context.During the late 1990s, insecticide resistance had rendered a number of treatment products ineffective; some companies saw this as an opportunity to develop alternative types of treatment. We investigated the possibility that a surfactant-based lotion containing 10% cocamide diethanolamine (cocamide DEA) was effective to eliminate head louse infestation.Settings and Design.Initialin vitrotesting of the lotion formulation versus laboratory reared body/clothing lice, followed by two randomised, controlled, community-based, assessor blinded, clinical studies.Materials and Methods.Preliminary laboratory tests were performed by exposing lice or louse eggs to the product using a method that mimicked the intended use. Clinical Study 1: Children and adults with confirmed head louse infestation were treated by investigators using a single application of aqueous 10% cocamide DEA lotion applied for 60 min followed by shampooing or a single 1% permethrin creme rinse treatment applied to pre-washed hair for 10 min. Clinical Study 2: Compared two treatment regimens using 10% cocamide DEA lotion that was concentrated by hair drying. A single application left on for 8 h/overnight was compared with two applications 7 days apart of 2 h duration, followed by a shampoo wash.Results.The initial laboratory tests showed a pediculicidal effect for a 60 min application but limited ovicidal effect. A longer application time of 8 h or overnight was found capable of killing all eggs but this differed between batches of test material. Clinical Study 1: Both treatments performed badly with only 3/23 (13%) successful treatments using cocamide DEA and 5/25 (23.8%) using permethrin. Clinical Study 2: The single overnight application of cocamide DEA concentrated by hair drying gave 10/56 (17.9%) successes compared with 19/56 (33.9%) for the 2 h application regimen repeated after 1 week. Intention to treat analysis showed no significant difference (p= 0.0523) between the treatments. Over the two studies, there were 18 adverse events possibly or probably associated with treatment, most of which were increased pruritus after treatment.Conclusions.Cocamide DEA 10% lotion, even when concentrated by hair drying, showed limited activity to eliminate head louse infestation.

scholarly journals Effect of Tannic Acid on Bond Strength of Etch and Rinse and Self-etch Adhesive Systems in Dentin of Primary Teeth

2017 ◽  
Vol 18 (1) ◽  
pp. 34-38
Author(s):  
Mojtaba Abdollahi ◽  
Masoumeh Ebrahimi ◽  
Alireza Sarraf Shirazi ◽  
Faraneh Abdolhoseinpour
Keyword(s):  
Bond Strength ◽  
Tannic Acid ◽  
Primary Teeth ◽  
Testing Machine ◽  
T Test ◽  
Adhesive Systems ◽  
Primary Molar ◽  
Etch And Rinse ◽  
Self Etch

ABSTRACT Introduction One of the possible mechanisms for the gradual destruction of bond strength in dentin-resin interface, could be due to the demineralized unstable collagen matrix. Use of protease inhibitors, such as tannic acid (TA) could prevent destruction of collagen fibers. The aim of this study was to compare the TA effect on bond strength of etch and rinse and self-etch adhesive systems in the dentin of primary teeth. Materials and methods This in vitro study was done on 40 extracted primary molar teeth. The teeth were sectioned in the mesiodistal direction, and enamel of buccal and lingual surfaces was removed. Samples were randomly divided into four groups: Single bond (SB) + TA, SB, Clearfil SE Bond (CSB) + TA, and CSB. Then, Z250 and Clearfil AP-X composites were cured on the surfaces of SB and CSB groups respectively. After that, all samples were divided into aging and non-aging groups. For 3 months, samples were placed under 1,000 thermal cycles in aging group. Subsequently, the shear bond strengths of all groups were measured by the International testing machine, and failure mode was evaluated by an optical stereomicroscope. Data were analyzed with paired t-test and independent t-test. Results Tannic acid induced a significant reduction in the immediate bond strength of adhesive SB. Meanwhile, TA had no significant effect on shear bond strength of the CSB system. Conclusion Based on our findings, use of TA is not recommended with SB and CSB adhesives on primary teeth. Clinical significance Tannic acid may not be considered in resin restorations of primary teeth. How to cite this article Ebrahimi M, Sarraf Shirazi A, Abdolhoseinpour F, Abdollahi M. Effect of Tannic Acid on Bond Strength of Etch and Rinse and Self-etch Adhesive Systems in Dentin of Primary Teeth. J Contemp Dent Pract 2017;18(1):34-38.

scholarly journals Comparación In vitro de la resistencia adhesiva de los sistemas adhesivos Etch and Rinse (grabado y enjuague) y self etch (Autograbado)

2018 ◽  
Vol 21 (1) ◽  
pp. 23
Author(s):  
Edwin Miranda
Keyword(s):  
Etch And Rinse ◽  
El Sistema ◽  
Self Etch

El propósito del estudio fue comparar in vitro la resistencia adhesiva de los sistemas Etch and Rinse (grabado y enjuague), 4ta y 5ta generación; y los sistemas Self Etch (autograbado), 6ta y 7ma generación. Se realizó un estudiodescriptivo, comparativo. Se utilizaron 20 terceros molares extraídos por motivos ortodóncico y profilácticos. Los cuales fueron cortados por la mitad obteniendo 40 muestras, en forma aleatoria los dientes fueron divididos en cuatro grupos, 10 con 4ta generación, 10 con 5ta generación, 10 con 6ta generación y 10 con 7ma generación. Se realizó una prueba de tracción vertical medido en kilogramos fuerza, para luego ser transformados a megapascales (Mpa). Las resinas de 4ta generación obtuvieron una resistencia adhesiva de 29,9 Mpa, las de 5ta una resistencia de 16,9 Mpa, la de 6ta una resistencia de 27,5 Mpa y las de 7ma generación una resistencia de 11,0 Mpa. Los resultados se sometieron a pruebas de normalidad mediante la prueba de Shapiro Wilk, los mismos que fueron analizados mediante el ANOVA de un factor, encontrándose diferencias estadísticamente significativas entre los cuatro grupos de estudio conun valor p de 0,001. Se concluye que el sistema adhesivo de 4ta generación clasificado como Etch and Rinse presento mejor resistencia adhesiva, seguido del sistema adhesivo de 6ta generación clasificado como Self Etch.

Catechin-based Dentin Pretreatment and the Clinical Performance of a Universal Adhesive: A Two-year Randomized Clinical Trial

2020 ◽  
Vol 45 (5) ◽  
pp. 473-483 ◽  
Author(s):  
CAGA Costa ◽  
NLG Albuquerque ◽  
JS Mendonça ◽  
AD Loguercio ◽  
VPA Saboia ◽  
...  
Keyword(s):  
Clinical Trial ◽  
Randomized Clinical Trial ◽  
Clinical Performance ◽  
Resin Composite ◽  
Retention Rates ◽  
Marginal Adaptation ◽  
Single Bond ◽  
Etch And Rinse ◽  
Significant Difference ◽  
Self Etch

Clinical Relevance At 24 months, the dentin pretreatment with epigallocatechin-3-gallate did not impair the clinical performance of the adhesive Single Bond Universal regardless of the bonding strategy used. SUMMARY Purpose: To evaluate the two-year effect of dentin pretreatment with epigallocatechin-3-gallate (EGCG) on the clinical performance of restorations of noncarious cervical lesions (NCCLs) with Single Bond Universal, applied in two different modes (self-etch and etch-and-rinse). Methods and Materials: In this randomized clinical trial, 33 volunteers were selected, and 156 NCCLs were assigned to four groups: ER, etch-and-rinse; ER-EGCG, 0.1% EGCG dentin pretreatment + etch-and-rinse; SE, self-etch; and SE-EGCG, 0.1% EGCG dentin pretreatment + self-etch. The NCCLs were restored with a nanofilled resin composite and evaluated at baseline and at six, 12, 18, and 24 months using FDI criteria for retention, marginal staining, marginal adaptation, caries, and postoperative sensitivity. Two evaluators were blinded to the treatments performed, and impressions were taken for resin replicas to allow indirect observations. Statistical analyses were performed with Kruskal-Wallis and McNemar tests with a significance level of 5%. Results: Six restorations (one from ER, two from SE, one from ER-EGCG, and two from SEEGCG) were lost at 24 months with no significant differences (p&gt;0.05). The retention rates were 97.0% (ER and ER-EGCG), 94.1% (SE), and 94.2% (SE-EGCG). For marginal adaptation, a significant difference was detected between the baseline and 24 months for the SE group (p=0.0313). There were no statistical differences among all other evaluated criteria at 24 months, neither for each group at baseline nor for 24-month comparisons (p&gt;0.05). Conclusions: The pretreatment with EGCG provided no benefit in the clinical performance of the adhesive regardless of the bonding strategy used. In addition, it adds an additional required step to the restorative procedure.

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