Effect of the Application of a Dehydrothermal Treatment on the Structure and the Mechanical Properties of Collagen Film

Xuefei Chen; Lingling Zhou; Huaizhong Xu; Masaki Yamamoto; Masaya Shinoda; Masanori Kishimoto; Tomonari Tanaka; Hideki Yamane

doi:10.3390/ma13020377

Effect of the Application of a Dehydrothermal Treatment on the Structure and the Mechanical Properties of Collagen Film

Materials ◽

10.3390/ma13020377 ◽

2020 ◽

Vol 13 (2) ◽

pp. 377 ◽

Cited By ~ 6

Author(s):

Xuefei Chen ◽

Lingling Zhou ◽

Huaizhong Xu ◽

Masaki Yamamoto ◽

Masaya Shinoda ◽

...

Keyword(s):

Mechanical Properties ◽

Self Assembly ◽

Triple Helix ◽

Treatment Time ◽

Covalent Bond ◽

Crosslinking Density ◽

Treatment Temperature ◽

Collagen Film ◽

Helix Structure ◽

Collagen Molecules

Dehydrothermal (DHT) treatment was used to improve the properties of collagen casings because of its non-cytotoxicity. Understanding the effects of DHT treatment on the structure and mechanical properties of collagen films is beneficial to developing satisfying collagen casings. Herein, DHT treatment with various temperatures (85–145 °C) and timescales (1–7 days) were investigated. It was clarified that the chemical crosslinking covalent bond between collagen molecules was formed after the DHT treatment. Crosslinking density increased with increasing DHT treatment temperatures, contributing to the increase of tensile strength up to over three times of that of the untreated collagen film. The increased crosslinking density was also found when increasing the DHT treatment time, and the maximum was obtained in 3 days. Further DHT treatment time did not change the crosslinking density. The damage in the triple helix structure and the self-assembly of collagen molecules were observed from IR and SAXS. The extent of denaturation increased with increasing DHT treatment temperature and time, although the effect of the DHT treatment time on the denaturation was more moderate. When the DHT treatment temperature was as high as 145 °C or the DHT treatment time exceeded 5 days, serious denaturation occurs, leading to the deterioration of mechanical properties.

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Preparation and Characterization of the Microspheres Comprised of Atelocollagen and BCP

Key Engineering Materials ◽

10.4028/www.scientific.net/kem.330-332.423 ◽

2007 ◽

Vol 330-332 ◽

pp. 423-426

Author(s):

Qing Rong Wei ◽

Jian Lu ◽

Hui Chuan Zhao ◽

Bo Jiang ◽

Bo Zhang ◽

...

Keyword(s):

Self Assembly ◽

Triple Helix ◽

Biphasic Calcium Phosphate ◽

Collagen Matrix ◽

Enzymatic Digestion ◽

Filling Material ◽

Helix Structure ◽

Collagen Molecules ◽

Bone Filling

In order to develop a bone-filling material with osteoinductive potential, a composite micorspheres of collagen molecules and biphasic calcium phosphate (BCP) was prepared by utilizing emulsion polymerization and the intrinsic self-assembly of collagen. The prepared microspheres were analyzed by granularity test, scanning electron microscopy (SEM), infrared spectra (IR) and enzymatic digestion experiment. The results showed that the collagen matrix of fibrils was reconstituted in the droplets, and the native triple-helix structure of collagen was still maintained. The study provides an effective way to prepare microspheres of collagen and BCP composite.

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Anionic surfactant sulfate dodecyl sodium (SDS)-induced thermodynamics and conformational changes of collagen by ultrasensitive microcalorimetry

Journal of Leather Science and Engineering ◽

10.1186/s42825-021-00063-2 ◽

2021 ◽

Vol 3 (1) ◽

Author(s):

Jie Zhang ◽

Chunhua Wang ◽

Fengteng Zhang ◽

Wei Lin

Keyword(s):

Thermal Stability ◽

Conformational Changes ◽

Triple Helix ◽

Striking Difference ◽

Thermal Transition ◽

Scanning Calorimetry ◽

Helix Structure ◽

Stabilizing Effect ◽

Collagen Molecules ◽

Thermal Stability Of

Abstract In this communication, sulfate dodecyl sodium (SDS)-induced thermodynamics and conformational changes of collagen were studied. We used ultrasensitive differential scanning calorimetry (US-DSC) to directly monitor the thermal transition of collagen in the presence of SDS. The results show that SDS affects the conformation and thermal stability of collagen very differently depending on its concentrations. At CSDS ≤ 0.05 mM, the enhanced thermal stability of collagen indicates the stabilizing effect by SDS. However, a further increase of SDS leads to the denaturation of collagen, verifying the well-known ability of SDS to unfold proteins. This striking difference in thermodynamics and conformational changes of collagen caused by SDS concentrations can be explained in terms of their interactions. With increasing SDS, the binding of SDS to collagen can be dominated by electrostatic interaction shifting to hydrophobic interaction, and the latter plays a key role in loosening and unfolding the triple-helix structure of collagen. The important finding in the present study is the stabilizing effect of SDS on collagen molecules at extreme low concentration. Graphical abstract

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Mapping the Mechanical Properties of Hierarchical Supercrystalline Ceramic-Organic Nanocomposites

Molecules ◽

10.3390/molecules25204790 ◽

2020 ◽

Vol 25 (20) ◽

pp. 4790 ◽

Cited By ~ 2

Author(s):

Büsra Bor ◽

Lydia Heilmann ◽

Berta Domènech ◽

Michael Kampferbeck ◽

Tobias Vossmeyer ◽

...

Keyword(s):

Mechanical Properties ◽

Heat Treatment ◽

Organic Phase ◽

Self Assembly ◽

Organic Content ◽

Treatment Temperature ◽

Covalent Bonds ◽

Soft Phase ◽

Beneficial Effects ◽

Ceramic Nanoparticles

Multiscale ceramic-organic supercrystalline nanocomposites with two levels of hierarchy have been developed via self-assembly with tailored content of the organic phase. These nanocomposites consist of organically functionalized ceramic nanoparticles forming supercrystalline micron-sized grains, which are in turn embedded in an organic-rich matrix. By applying an additional heat treatment step at mild temperatures (250–350 °C), the mechanical properties of the hierarchical nanocomposites are here enhanced. The heat treatment leads to partial removal and crosslinking of the organic phase, minimizing the volume occupied by the nanocomposites’ soft phase and triggering the formation of covalent bonds through the organic ligands interfacing the ceramic nanoparticles. Elastic modulus and hardness up to 45 and 2.5 GPa are attained, while the hierarchical microstructure is preserved. The presence of an organic phase between the supercrystalline grains provides a toughening effect, by curbing indentation-induced cracks. A mapping of the nanocomposites’ mechanical properties reveals the presence of multiple microstructural features and how they evolve with heat treatment temperature. A comparison with non-hierarchical, homogeneous supercrystalline nanocomposites with lower organic content confirms how the hierarchy-inducing organic excess results in toughening, while maintaining the beneficial effects of crosslinking on the materials’ stiffness and hardness.

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The Characteristics of Intrinsic Fluorescence of Type I Collagen Influenced by Collagenase I

Applied Sciences ◽

10.3390/app8101947 ◽

2018 ◽

Vol 8 (10) ◽

pp. 1947 ◽

Cited By ~ 3

Author(s):

Yiming Shen ◽

Deyi Zhu ◽

Wenhui Lu ◽

Bing Liu ◽

Yanchun Li ◽

...

Keyword(s):

Fluorescence Intensity ◽

Self Assembly ◽

Triple Helix ◽

Type I Collagen ◽

Intrinsic Fluorescence ◽

Type I ◽

Food And Agriculture ◽

Mechanism Research ◽

Helix Structure ◽

Assembly Behavior

The triple helix structure of collagen can be degraded by collagenase. In this study, we explored how the intrinsic fluorescence of type I collagen was influenced by collagenase I. We found that tyrosine was the main factor that could successfully excite the collagen fluorescence. Initially, self-assembly behavior of collagen resulted in a large amount of tyrosine wrapped with collagen, which decreased the fluorescence intensity of type I collagen. After collagenase cleavage, some wrapped-tyrosine could be exposed and thereby the intrinsic fluorescence intensity of collagen increased. By observation and analysis, the influence of collagenase to intrinsic fluorescence of collagen was investigated and elaborated. Furthermore, collagenase cleavage to the special triple helix structure of collagen would result in a slight improvement of collagen thermostability, which was explained by the increasing amount of terminal peptides. These results are helpful and effective for reaction mechanism research related to collagen, which can be observed by fluorescent technology. Meantime, the reaction behaviors of both collagenase and collagenolytic proteases can also be analyzed by fluorescent technology. In conclusion, this research provides a foundation for the further investigation of collagen reactions in different areas, such as medicine, nutrition, food and agriculture.

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Research on Microstructure and Mechanical Properties Evolution of Rheocast and Thixocast 319s Aluminum Alloy

Materials Science Forum ◽

10.4028/www.scientific.net/msf.850.802 ◽

2016 ◽

Vol 850 ◽

pp. 802-808 ◽

Cited By ~ 1

Author(s):

Kang Du ◽

Xiao Kang Liang ◽

Da Quan Li ◽

Qiang Zhu

Keyword(s):

Mechanical Properties ◽

Heat Treatment ◽

Treatment Time ◽

Eutectic Silicon ◽

Solution Treatment ◽

Treatment Temperature ◽

Solid Alloy ◽

Obvious Effect ◽

Higher Temperature ◽

Semi Solid

In semi-solid rheocast and thixocast industry, T6 heat treatment was one key factor to improve the mechanical properties of the castings. The microstructure evolution was closely influenced by heat treatment temperature and time. In this paper, the morphology change of eutectic silicon in semi-solid alloy during different heat treatment time was firstly observed. The changes of both roundness and aspect show that the silicon particles underwent fragmentation, coarsening and growing up processes during solution treatment. Then, the mechanical properties after stand T6 and T6 with higher temperature were compared. It may be concluded that the higher temperature doesn’t have obvious effect to increase the mechanical strength, but severe negative effect on the elongation. Finally, the incipient melting defect appeared in higher temperature T6 was proved and its relationship with elongation was analysed.

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Effect of High Temperature on the Change in Color, Dimensional Stability and Mechanical Properties of Spruce Wood

Holzforschung ◽

10.1515/hf.2003.080 ◽

2003 ◽

Vol 57 (5) ◽

pp. 539-546 ◽

Cited By ~ 300

Author(s):

P. Bekhta ◽

P. Niemz

Keyword(s):

Mechanical Properties ◽

Heat Treatment ◽

High Temperature ◽

Dimensional Stability ◽

Modulus Of Elasticity ◽

Bending Strength ◽

Treatment Time ◽

Color Difference ◽

Treatment Temperature ◽

Color Changes

Summary In this study the effect of high temperature on mechanical properties, dimensional stability and color of spruce was investigated. Wood specimens conditioned at different relative humidities (50, 65, 80 and 95%) were subjected to heat treatment at 200°C for 2, 4, 8, 10 and 24 h and at 100, 150 and 200°C for 24 h. Color changes were measured in the Minolta Croma-Meter CR-300 color system. Bending strength and modulus of elasticity were determined according to DIN 52186. The results show that heat treatment mainly resulted in a darkening of wood tissues, improvement of the dimensional stability of wood and reduction of its mechanical properties. The darkening accelerated generally when treatment temperature exceeded approximately 200°C. Most of the darkening occurred within the first 4 h of exposure. For the specimens heated to high temperatures, the average decrease in bending strength was about 44–50%, while modulus of elasticity was reduced by only 4–9%. We found that treatment time and temperature were more important than relative humidity regarding the color responses. Strong correlations between total color difference and both modulus of elasticity and bending strength were found. Thus, the color parameters can be estimated quantitatively and used as a prediction of wood strength.

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An experimentally informed statistical elasto-plastic mineralised collagen fibre model at the micrometre and nanometre lengthscale

Scientific Reports ◽

10.1038/s41598-021-93505-0 ◽

2021 ◽

Vol 11 (1) ◽

Author(s):

Alexander Groetsch ◽

Philippe K. Zysset ◽

Peter Varga ◽

Alexandra Pacureanu ◽

Françoise Peyrin ◽

...

Keyword(s):

Mechanical Properties ◽

Self Assembly ◽

Load Transfer ◽

Collagen Fibre ◽

High Strength ◽

X Ray Scattering ◽

Yield Behaviour ◽

Nanoscale Imaging ◽

Collagen Molecules ◽

Inorganic Mineral

AbstractBone is an intriguingly complex material. It combines high strength, toughness and lightweight via an elaborate hierarchical structure. This structure results from a biologically driven self-assembly and self-organisation, and leads to different deformation mechanisms along the length scales. Characterising multiscale bone mechanics is fundamental to better understand these mechanisms including changes due to bone-related diseases. It also guides us in the design of new bio-inspired materials. A key-gap in understanding bone’s behaviour exists for its fundamental mechanical unit, the mineralised collagen fibre, a composite of organic collagen molecules and inorganic mineral nanocrystals. Here, we report an experimentally informed statistical elasto-plastic model to explain the fibre behaviour including the nanoscale interplay and load transfer with its main mechanical components. We utilise data from synchrotron nanoscale imaging, and combined micropillar compression and synchrotron X-ray scattering to develop the model. We see that a 10-15% micro- and nanomechanical heterogeneity in mechanical properties is essential to promote the ductile microscale behaviour preventing an abrupt overall failure even when individual fibrils have failed. We see that mineral particles take up 45% of strain compared to collagen molecules while interfibrillar shearing seems to enable the ductile post-yield behaviour. Our results suggest that a change in mineralisation and fibril-to-matrix interaction leads to different mechanical properties among mineralised tissues. Our model operates at crystalline-, molecular- and continuum-levels and sheds light on the micro- and nanoscale deformation of fibril-matrix reinforced composites.

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Preparation and Mechanical Properties of Polyurethane Elastomer with Two Soft Segments of HTPB/PTMG

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.150-151.1689 ◽

2010 ◽

Vol 150-151 ◽

pp. 1689-1692 ◽

Cited By ~ 1

Author(s):

Hai Tao Tao ◽

Xi Jun Liu ◽

Tie Ning Ma

Keyword(s):

Mechanical Properties ◽

Heat Treatment ◽

Molecular Weight ◽

Treatment Time ◽

Treatment Temperature ◽

Chain Extender ◽

Toluene Diisocyanate ◽

Molar Ratio ◽

Mass Ratio ◽

Polyurethane Elastomers

In this paper, a series of polyurethane elastomers (PUE) were prepared employing casting method using toluene diisocyanate (TDI), hydroxy-terminated butadiene-acrylonitrile copolymer(HTBN) and polytetrahydrofuran glycol (PTMG) as the main raw materials, and using 2,4- and 2,6- dimethylthioaromatic diamine (DMTDA) as a chain extender. The effects of the content of NCO in PUE (NCO%), mass ratio of HTBN/PTMG, molecular weight of PTMG, dosage of chain extender and heat treatment on the mechanical properties of PUE were studied. The results showed that the lower molecular weight of PTMG and the higher heat treatment temperature were both favorable for increasing the mechanical properties of PUE. When the mass ratio of HTBN/PTMG was 35:65, NCO% was 6.0%, molar ratio of NCO/NH2 was 1.20 and heat treatment time was 2h at 115 , the mechanical properties of PUE were best.

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Nanostructure, Self-Assembly, Mechanical Properties, and Antioxidant Activity of a Lupin-Derived Peptide Hydrogel

Biomedicines ◽

10.3390/biomedicines9030294 ◽

2021 ◽

Vol 9 (3) ◽

pp. 294

Author(s):

Raffaele Pugliese ◽

Anna Arnoldi ◽

Carmen Lammi

Keyword(s):

Mechanical Properties ◽

Antioxidant Activity ◽

Self Assembly ◽

Antioxidant Activities ◽

Functional Materials ◽

Cd Spectroscopy ◽

Beneficial Effects ◽

Naturally Occurring ◽

New Research ◽

Peptide Hydrogel

Naturally occurring food peptides are frequently used in the life sciences due to their beneficial effects through their impact on specific biochemical pathways. Furthermore, they are often leveraged for applications in areas as diverse as bioengineering, medicine, agriculture, and even fashion. However, progress toward understanding their self-assembling properties as functional materials are often hindered by their long aromatic and charged residue-enriched sequences encrypted in the parent protein sequence. In this study, we elucidate the nanostructure and the hierarchical self-assembly propensity of a lupin-derived peptide which belongs to the α-conglutin (11S globulin, legumin-like protein), with a straightforward N-terminal biotinylated oligoglycine tag-based methodology for controlling the nanostructures, biomechanics, and biological features. Extensive characterization was performed via Circular Dichroism (CD) spectroscopy, Fourier Transform Infrared spectroscopy (FT-IR), rheological measurements, and Atomic Force Microscopy (AFM) analyses. By using the biotin tag, we obtained a thixotropic lupin-derived peptide hydrogel (named BT13) with tunable mechanical properties (from 2 to 11 kPa), without impairing its spontaneous formation of β-sheet secondary structures. Lastly, we demonstrated that this hydrogel has antioxidant activity. Altogether, our findings address multiple challenges associated with the development of naturally occurring food peptide-based hydrogels, offering a new tool to both fine tune the mechanical properties and tailor the antioxidant activities, providing new research directions across food chemistry, biochemistry, and bioengineering.

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