scholarly journals Efficacy of Bacterial Cellulose as a Carrier of BMP-2 for Bone Regeneration in a Rabbit Frontal Sinus Model

Materials ◽  
2019 ◽  
Vol 12 (15) ◽  
pp. 2489 ◽  
Author(s):  
Takashi Koike ◽  
Jingjing Sha ◽  
Yunpeng Bai ◽  
Yuhei Matsuda ◽  
Katsumi Hideshima ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bacterial Cellulose ◽  
Alveolar Bone ◽  
Dental Treatment ◽  
Bone Morphogenetic Protein 2 ◽  
Nuclear Antigen ◽  
Bone Augmentation ◽  
Morphogenetic Protein ◽  
Maxillary Molar ◽  
Proliferating Cell

If the alveolar bone height of patients requiring dental implants in the maxillary molar region is inadequate, it is difficult to achieve satisfactory outcomes using existing bone graft materials. We previously reported the possible utility of bacterial cellulose (BC) as a new dental treatment material. BC has a high absorptive capacity, good mechanical strength, and good volume retention. BC loaded with bone morphogenetic protein-2 (BMP-2) might allow effective alveolar bone augmentation. We created critical frontal bone defect models in 12 male Japanese white rabbits and divided them into four groups: sham; BC (BC grafting only); BMP-2 (treated with BMP-2 solution only); and BC+BMP-2 (grafted with BC loaded with BMP-2). Newly formed bone volume was calculated via hematoxylin-eosin staining evaluation. The proliferating cell nuclear antigen and osteocalcin levels were determined by the immunohistochemical staining analysis. All measured indices of the BC+BMP-2 group were significantly superior to those of the other groups (all p < 0.05). BC maintained the graft space and released BMP-2 in a sustained manner, promoting optimal bone formation. The BC+BMP-2 combination enhanced bone regeneration and shows promise as a useful means of clinical pre-dental implant bone augmentation in the maxillary sinus.

scholarly journals Bone Regeneration Using a Three-Dimensional Hexahedron Channeled BCP Block Combined with Bone Morphogenic Protein-2 in Rat Calvarial Defects

Materials ◽  
2019 ◽  
Vol 12 (15) ◽  
pp. 2435 ◽  
Author(s):  
So-Yeun Kim ◽  
Eun-Bin Bae ◽  
Jae-Woong Huh ◽  
Jong-Ju Ahn ◽  
Hyun-Young Bae ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Alveolar Bone ◽  
Three Dimensional ◽  
Bone Morphogenetic Protein 2 ◽  
Graft Material ◽  
Implant Placement ◽  
Morphogenetic Protein ◽  
Human Bone Morphogenetic Protein ◽  
Calvarial Defects ◽  
Rat Calvarial Defect

It is important to obtain sufficient bone mass before implant placement on alveolar bone, and synthetic bone such as biphasic calcium phosphate (BCP) has been studied to secure this. This study used a BCP block bone with a specific structure of the three-dimensional (3D) hexahedron channel and coating with recombinant human bone morphogenetic protein-2 (rhBMP-2) impregnated carboxymethyl cellulose (CMC) was used to examine the enhancement of bone regeneration of this biomaterial in rat calvarial defect. After the preparation of critical-size calvarial defects in fifteen rats, defects were divided into three groups and were implanted with the assigned specimen (n = 5): Boneplant (untreated 3D hexahedron channeled BCP block), Boneplant/CMC (3D hexahedron channeled BCP block coated with CMC), and Boneplant/CMC/BMP (3D hexahedron channeled BCP block coated with CMC containing rhBMP-2). After 4 weeks, the volumetric, histologic, and histometric analyses were conducted to measure the newly formed bone. Histologically, defects in the Boneplant/CMC/BMP group were almost completely filled with new bone compared to the Boneplant and Boneplant/CMC groups. The new bone volume (P < 0.05) and area (P < 0.001) in the Boneplant/CMC/BMP group (20.12% ± 2.17, 33.79% ± 3.66) were much greater than those in the Boneplant (10.77% ± 4.8, 16.48% ± 9.11) and Boneplant/CMC (10.72% ± 3.29, 16.57% ± 8.94) groups, respectively. In conclusion, the 3D hexahedron channeled BCP block adapted rhBMP-2 with carrier CMC showed high possibility as an effective bone graft material.

scholarly journals Polyethylenimine-alginate nanocomposites based bone morphogenetic protein 2 gene-activated matrix for alveolar bone regeneration

RSC Advances ◽  
2019 ◽  
Vol 9 (46) ◽  
pp. 26598-26608 ◽  
Author(s):  
Han Jin ◽  
Zhongshuang Liu ◽  
Wei Li ◽  
Zhuling Jiang ◽  
Ying Li ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bone Morphogenetic Protein ◽  
Alveolar Bone ◽  
Bone Morphogenetic Protein 2 ◽  
Alveolar Ridge ◽  
Morphogenetic Protein ◽  
Valuable Method

Polyethylenimine-alginate nanocomposites based bone morphogenetic protein 2 gene-activated matrix may provide an easy and valuable method for alveolar ridge regeneration.

scholarly journals Bacterial cellulose membrane functionalized with hydroxiapatite and anti-bone morphogenetic protein 2: A promising material for bone regeneration

PLoS ONE ◽  
2019 ◽  
Vol 14 (8) ◽  
pp. e0221286 ◽  
Author(s):  
Fernanda Coelho ◽  
Maurício Cavicchioli ◽  
Sybele Saska Specian ◽  
Raquel Mantuaneli Scarel-Caminaga ◽  
Letícia de Aquino Penteado ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Bacterial Cellulose ◽  
Bone Morphogenetic Protein ◽  
Promising Material ◽  
Bone Morphogenetic Protein 2 ◽  
Cellulose Membrane ◽  
Morphogenetic Protein ◽  
Bacterial Cellulose Membrane

Non-Surgical Model for Alveolar Bone Regeneration by Bone Morphogenetic Protein-2/7 Gene Therapy

2017 ◽  
pp. 1-18 ◽  
Author(s):  
Mariko Kawai ◽  
Yo-Hei Kataoka ◽  
Junya Sonobe ◽  
Hiromitsu Yamamoto ◽  
Masakazu Inubushi ◽  
...  
Keyword(s):  
Gene Therapy ◽  
Bone Regeneration ◽  
Bone Morphogenetic Protein ◽  
Alveolar Bone ◽  
Bone Morphogenetic Protein 2 ◽  
Surgical Model ◽  
Morphogenetic Protein

scholarly journals The Bone Regeneration Capacity of BMP-2 + MMP-10 Loaded Scaffolds Depends on the Tissue Status

Pharmaceutics ◽  
2021 ◽  
Vol 13 (7) ◽  
pp. 979
Author(s):  
Patricia Garcia-Garcia ◽  
Ricardo Reyes ◽  
José Antonio Rodriguez ◽  
Tomas Martín ◽  
Carmen Evora ◽  
...  
Keyword(s):  
Bone Formation ◽  
Bone Regeneration ◽  
Growth Promotion ◽  
Tissue Growth ◽  
Bone Morphogenetic Protein 2 ◽  
Morphogenetic Protein ◽  
Therapeutic Molecules ◽  
Positive Effect

Biomaterials-mediated bone formation in osteoporosis (OP) is challenging as it requires tissue growth promotion and adequate mineralization. Based on our previous findings, the development of scaffolds combining bone morphogenetic protein 2 (BMP-2) and matrix metalloproteinase 10 (MMP-10) shows promise for OP management. To test our hypothesis, scaffolds containing BMP-2 + MMP-10 at variable ratios or BMP-2 + Alendronate (ALD) were prepared. Systems were characterized and tested in vitro on healthy and OP mesenchymal stem cells and in vivo bone formation was studied on healthy and OP animals. Therapeutic molecules were efficiently encapsulated into PLGA microspheres and embedded into chitosan foams. The use of PLGA (poly(lactic-co-glycolic acid)) microspheres as therapeutic molecule reservoirs allowed them to achieve an in vitro and in vivo controlled release. A beneficial effect on the alkaline phosphatase activity of non-OP cells was observed for both combinations when compared with BMP-2 alone. This effect was not detected on OP cells where all treatments promoted a similar increase in ALP activity compared with control. The in vivo results indicated a positive effect of the BMP-2 + MMP-10 combination at both of the doses tested on tissue repair for OP mice while it had the opposite effect on non-OP animals. This fact can be explained by the scaffold’s slow-release rate and degradation that could be beneficial for delayed bone regeneration conditions but had the reverse effect on healthy animals. Therefore, the development of adequate scaffolds for bone regeneration requires consideration of the tissue catabolic/anabolic balance to obtain biomaterials with degradation/release behaviors suited for the existing tissue status.

Design of hydroxyapatite bioceramics with micro-/nano-topographies to regulate the osteogenic activities of bone morphogenetic protein-2 and bone marrow stromal cells

Nanoscale ◽  
2020 ◽  
Vol 12 (13) ◽  
pp. 7284-7300 ◽  
Author(s):  
Xiangfeng Li ◽  
Minjun Liu ◽  
Fuying Chen ◽  
Yuyi Wang ◽  
Menglu Wang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Regeneration ◽  
Bone Morphogenetic Protein ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Bone Morphogenetic Protein 2 ◽  
Natural Bone ◽  
Morphogenetic Protein ◽  
Bone Apatite

Biomimicking the nanostructure of natural bone apatite to enhance the bioactivity of hydroxyapatite (HA) biomaterials is an eternal topic in the bone regeneration field.

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