scholarly journals A New Bioactive Glass/Collagen Hybrid Composite for Applications in Dentistry

Materials ◽  
2019 ◽  
Vol 12 (13) ◽  
pp. 2079 ◽  
Author(s):  
Devis Bellucci ◽  
Roberta Salvatori ◽  
Jessica Giannatiempo ◽  
Alexandre Anesi ◽  
Sergio Bortolini ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Gold Standard ◽  
Collagen Matrix ◽  
Bioactive Glasses ◽  
Wide Range ◽  
Murine Fibroblasts ◽  
45S5 Bioglass ◽  
Dental Applications ◽  
Stimulate Cell Proliferation

Bioactive glasses (BGs) are currently employed in a wide range of medical and dentistry applications by virtue of their bone-bonding ability. The incorporation of BGs into a collagen matrix may be used to combine the regenerative potential of these materials with the specific biological advantages of collagen. However, most of the collagen/BG composites reported in the literature are scaffolds and there is a lack of moldable putties or injectable systems. Here, granules of an innovative BG containing strontium and magnesium were mixed with collagen and PEG to obtain a putty (BGMS/C) suitable for dental applications. For the sake of comparison, granules of 45S5 Bioglass®, the gold standard among BGs, were used to prepare a 45S5/collagen putty. Both the composites were evaluated in vitro with respect to murine fibroblasts. The materials showed an excellent biocompatibility, making them interesting for possible applications in dentistry and reconstructive surgery. Moreover, BGMS/C seems to stimulate cell proliferation.

scholarly journals Mesoporous Silica Nanoparticles and Mesoporous Bioactive Glasses for Wound Management: From Skin Regeneration to Cancer Therapy

Materials ◽  
2021 ◽  
Vol 14 (12) ◽  
pp. 3337
Author(s):  
Sara Hooshmand ◽  
Sahar Mollazadeh ◽  
Negar Akrami ◽  
Mehrnoosh Ghanad ◽  
Ahmed El-Fiqi ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Silica Nanoparticles ◽  
Mesoporous Silica Nanoparticles ◽  
Skin Regeneration ◽  
Bioactive Molecules ◽  
Wound Management ◽  
Bioactive Glasses ◽  
Wide Range

Exploring new therapies for managing skin wounds is under progress and, in this regard, mesoporous silica nanoparticles (MSNs) and mesoporous bioactive glasses (MBGs) offer great opportunities in treating acute, chronic, and malignant wounds. In general, therapeutic effectiveness of both MSNs and MBGs in different formulations (fine powder, fibers, composites etc.) has been proved over all the four stages of normal wound healing including hemostasis, inflammation, proliferation, and remodeling. The main merits of these porous substances can be summarized as their excellent biocompatibility and the ability of loading and delivering a wide range of both hydrophobic and hydrophilic bioactive molecules and chemicals. In addition, doping with inorganic elements (e.g., Cu, Ga, and Ta) into MSNs and MBGs structure is a feasible and practical approach to prepare customized materials for improved skin regeneration. Nowadays, MSNs and MBGs could be utilized in the concept of targeted therapy of skin malignancies (e.g., melanoma) by grafting of specific ligands. Since potential effects of various parameters including the chemical composition, particle size/morphology, textural properties, and surface chemistry should be comprehensively determined via cellular in vitro and in vivo assays, it seems still too early to draw a conclusion on ultimate efficacy of MSNs and MBGs in skin regeneration. In this regard, there are some concerns over the final fate of MSNs and MBGs in the wound site plus optimal dosages for achieving the best outcomes that deserve careful investigation in the future.

Equine limb and oral fibroblasts in in vitro models of cell proliferation and wound contraction

2009 ◽  
Vol 2009 ◽  
pp. 19-19
Author(s):  
E Watts ◽  
M T Rose
Keyword(s):  
Cell Proliferation ◽  
Wound Healing ◽  
Granulation Tissue ◽  
Collagen Matrix ◽  
In Vitro Models ◽  
Scar Formation ◽  
Wound Contraction

Wound healing in horses is particularly problematic compared to other species and limb wounds often exhibit complications such as exuberant granulation tissue, poor wound contraction and unsightly scars. In comparison, oral wounds heal without scar formation. Therefore, in vitro experiments were conducted to investigate the differences between equine oral and limb fibroblasts in terms of proliferation and their ability to contract a collagen matrix, a commonly used in vitro model of wound contraction.

scholarly journals On the in Vitro Biocompatibility Testing of Bioactive Glasses

Materials ◽  
2020 ◽  
Vol 13 (8) ◽  
pp. 1816 ◽  
Author(s):  
Devis Bellucci ◽  
Elena Veronesi ◽  
Massimo Dominici ◽  
Valeria Cannillo
Keyword(s):  
Predictive Tool ◽  
Bioactive Glasses ◽  
Bioactive Materials ◽  
In Vitro Biocompatibility ◽  
Marrow Mesenchymal Stem Cells ◽  
Biocompatibility Testing ◽  
45S5 Bioglass ◽  
First Time

In this work, a new 3D cellular model—based on human bone marrow mesenchymal stem cells (BM–MSCs)—was used for the first time to test the 45S5 Bioglass® (45S5). Such a model, previously used to evaluate the biologic performance of two novel bioactive glasses, suggested out the regenerative potential of such materials. In fact, BM–MSCs were able both to adhere and colonize the biomaterials, as well as differentiate toward osteoblasts—even in absence of specific growth factors. Surprisingly, BM–MSCs were not able to colonize 45S5 granules (almost no adhesion and/or colonization was observed), and thus, were not capable of providing any osteogenic differentiation. Therefore, the model seems to suggest that the two novel bioactive glasses have a better biologic performance than 45S5. If this hypothesis is confirmed also by in vivo tests, the 3D model may become a predictive tool for discriminating between different potential bioactive materials by comparatively evaluating them, and preliminarily selecting the best ones in relation to their biocompatibility potential—before proceeding with further experiments in vivo. This approach could favor the reduction of costs and time of pre-clinical and clinical trials.

scholarly journals Osteogenic Effect of ZnO-Mesoporous Glasses Loaded with Osteostatin

Nanomaterials ◽  
2018 ◽  
Vol 8 (8) ◽  
pp. 592 ◽  
Author(s):  
Rebeca Pérez ◽  
Sandra Sanchez-Salcedo ◽  
Daniel Lozano ◽  
Clara Heras ◽  
Pedro Esbrit ◽  
...  
Keyword(s):  
Bone Growth ◽  
Textural Properties ◽  
New Bone Formation ◽  
Bioactive Glasses ◽  
Bioactive Materials ◽  
Parathyroid Hormone Related Protein ◽  
Wide Range ◽  
Before And After ◽  
Osteogenic Effect

Mesoporous Bioactive Glasses (MBGs) are a family of bioceramics widely investigated for their putative clinical use as scaffolds for bone regeneration. Their outstanding textural properties allow for high bioactivity when compared with other bioactive materials. Moreover, their great pore volumes allow these glasses to be loaded with a wide range of biomolecules to stimulate new bone formation. In this study, an MBG with a composition, in mol%, of 80% SiO2–15% CaO–5% P2O5 (Blank, BL) was compared with two analogous glasses containing 4% and 5% of ZnO (4ZN and 5ZN) before and after impregnation with osteostatin, a C-terminal peptide from a parathyroid hormone-related protein (PTHrP107-111). Zn2+ ions were included in the glass for their bone growth stimulator properties, whereas osteostatin was added for its osteogenic properties. Glasses were characterized, and their cytocompatibility investigated, in pre-osteoblastic MC3T3-E1 cell cultures. The simultaneous additions of osteostatin and Zn2+ ions provoked enhanced MC3T3-E1 cell viability and a higher differentiation capacity, compared with either raw BL or MBGs supplemented only with osteostatin or Zn2+. These in vitro results show that osteostatin enhances the osteogenic effect of Zn2+-enriched glasses, suggesting the potential of this combined approach in bone tissue engineering applications.

Adenosine and hypoxia stimulate proliferation and migration of endothelial cells

1988 ◽  
Vol 255 (3) ◽  
pp. H554-H562 ◽  
Author(s):  
C. J. Meininger ◽  
M. E. Schelling ◽  
H. J. Granger
Keyword(s):  
Cell Proliferation ◽  
Endothelial Cells ◽  
Culture Medium ◽  
Culture Conditions ◽  
Hypoxic Conditions ◽  
Cell Counts ◽  
And Migration ◽  
Stimulate Cell Proliferation ◽  
Proliferation And Migration

The proliferation of bovine aortic or coronary venular endothelial cells (EC) in vitro was stimulated by the addition of adenosine (0.5 or 5.0 microM) to the culture medium. Cell counts of adenosine-treated aortic EC were 23–76% and coronary venular EC 19–52% greater than nontreated controls. Because adenosine is known to be released by hypoxic tissues, cell proliferation was quantitated when aortic EC were grown at 2% O2. Cell counts were 41–102% greater under hypoxic conditions than when cells were grown at standard tissue culture conditions (approximately 20% O2). When culture medium conditioned by coronary EC grown at 2% O2 was added to EC growing at standard conditions, cell counts were 24–69% greater than controls with medium conditioned by coronary EC grown at 20% O2. This suggests that hypoxia causes endothelial cells to release a factor(s) into the medium that can stimulate cell proliferation. The addition of the adenosine receptor blocker 8-phenyltheophylline (10(-5) M) prevented the stimulation of proliferation caused by hypoxia-conditioned medium, 2% O2 or 5.0 microM adenosine, suggesting that adenosine mediates its effect via an external membrane receptor. Adenosine also stimulated EC chemotaxis. Taken together, these results suggest that adenosine, released as a result of tissue hypoxia, may act as an angiogenic stimulus for the growth of new blood vessels.

Osteoblast Behaviour on Nanostructured Ti-Bioceramic Composites

2011 ◽  
Vol 674 ◽  
pp. 153-158 ◽  
Author(s):  
Karolina Jurczyk ◽  
Katarzyna Niespodziana ◽  
M.U. Jurczyk ◽  
Mieczyslaw Jurczyk
Keyword(s):  
Mechanical Properties ◽  
Corrosion Resistance ◽  
Biological Properties ◽  
Grain Structure ◽  
Good Adhesion ◽  
Ti Alloys ◽  
Mechanical Properties And Corrosion ◽  
45S5 Bioglass ◽  
Dental Applications

Ti and Ti-based alloys are preferred materials in the production of implants in both medical and dental applications. One of the methods that allow the change of biological properties of Ti alloys is the modification of their chemical composition and microstructure. In this study, new biocompatible, nanostructured Ti-x vol% SiO2, Ti-x vol% 45S5 Bioglass, and Ti-x vol% HAp (x=0, 3, 10) materials have been developed, manufactured and studied in terms of their biocompatibility. These materials give the possibility of controlling in detail the grain structure and the composition of the alloy and, consequently, the mechanical and biocompatibility performances. Our results of in vitro studies show that these bionanocomposites have excellent biocompatibility and could integrate with bone. After 1st day of incubation cells show good adhesion to the surface of studied samples in the form of filopodia. After 5 days of incubation, the typical monolayer was observed. With regard to microcrystalline Ti it could help to obtain better dental implants with better mechanical properties and corrosion resistance.

scholarly journals Dolomite-Foamed Bioactive Silicate Scaffolds for Bone Tissue Repair

Materials ◽  
2020 ◽  
Vol 13 (3) ◽  
pp. 628 ◽  
Author(s):  
Elisa Fiume ◽  
Dilshat Tulyaganov ◽  
Graziano Ubertalli ◽  
Enrica Verné ◽  
Francesco Baino
Keyword(s):  
Three Dimensional ◽  
Bone Grafts ◽  
Structural Features ◽  
Tissue Growth ◽  
Bioactive Glasses ◽  
Easy Method ◽  
Wide Range ◽  
Minimum Requirements ◽  
Manufacturing Techniques

The use of three-dimensional (3D) scaffolds is recognized worldwide as a valuable biomedical approach for promoting tissue regeneration in critical-size bone defects. Over the last 50 years, bioactive glasses have been intensively investigated in a wide range of different clinical applications, from orthopedics to soft tissue healing. Bioactive glasses exhibit the unique capability to chemically bond to the host tissue and, furthermore, their processing versatility makes them very appealing due to the availability of different manufacturing techniques for the production of porous and interconnected synthetic bone grafts able to support new tissue growth over the whole duration of the treatment. As a novel contribution to the broad field of scaffold manufacturing, we report here an effective and relatively easy method to produce silicate glass-derived scaffolds by using, for the first time in the biomedical field, dolomite powder as a foaming agent for the formation of 3D bone-like porous structures. Morphological/structural features, crystallization behavior, and in vitro bioactivity in a simulated body fluid (SBF) were investigated. All the tested scaffolds were found to fulfil the minimum requirements that a scaffold for osseous repair should exhibit, including porosity (65–83 vol.%) and compressive strength (1.3–3.9 MPa) comparable to those of cancellous bone, as well as hydroxyapatite-forming ability (bioactivity). This study proves the suitability of a dolomite-foaming method for the production of potentially suitable bone grafts based on bioactive glass systems.

scholarly journals An In Vitro Model of Diabetic Retinal Vascular Endothelial Dysfunction and Neuroretinal Degeneration

2021 ◽  
Vol 2021 ◽  
pp. 1-12
Author(s):  
Qiyun Wang ◽  
Xinyuan Zhang ◽  
Kaiyue Wang ◽  
Ling Zhu ◽  
Bingjie Qiu ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Endothelial Dysfunction ◽  
Vascular Endothelial Cells ◽  
Tube Formation ◽  
Cell Counting ◽  
Vascular Endothelial ◽  
Migration Assay ◽  
Tube Formation Assay ◽  
Wide Range

Background. Diabetic retinopathy (DR) is a leading cause of blindness in working-age populations. Proper in vitro DR models are crucial for exploring pathophysiology and identifying novel therapeutic targets. This study establishes a rational in vitro diabetic retinal neuronal-endothelial dysfunction model and a comprehensive downstream validation system. Methods. Human retinal vascular endothelial cells (HRMECs) and retinal ganglion cells (RGCs) were treated with different glucose concentrations with mannitol as matched osmotic controls. Cell proliferation and viability were evaluated by the Cell Counting Kit-8. Cell migration was measured using a transwell migration assay. Cell sprouting was assessed by a tube formation assay. The VEGF expression was assessed by ELISA. RGCs were labeled by neurons and RGC markers TUJ1 and BRN3A for quantitative and morphological analysis. Apoptosis was detected using PI/Hoechst staining and TUNEL assay and quantified by ImageJ. Results. Cell proliferation and migration in HRMECs were significantly higher in the 25 mM glucose-treated group ( p < 0.001 ) but lower in the 50 mM and 100 mM groups ( p < 0.001 ). The permeability and the apoptotic index in HRMECs were statistically higher in the 25 mM, 50 mM, and 100 mM groups ( p < 0.05 ). The tube formation assay found that all the parameters were significantly higher in the 25 mM and 50 mM groups ( p < 0.001 ) concomitant with the elevated VEGFA expression in HRMECs ( p = 0.016 ). Cell viability was significantly lower in the 50 mM, 100 mM, and 150 mM groups in RGCs ( p 50 mM = 0.013 , p 100 mM = 0.019 , and p 150 mM = 0.002 ). Apoptosis was significantly elevated, but the proportion of RGCs with neurite extension was significantly lower in the 50 mM, 100 mM, and 150 mM groups ( p 50 mM < 0.001 , p 100 m M < 0.001 , and p 150 mM < 0.001 ). Conclusions. We have optimized glucose concentrations to model diabetic retinal endothelial (25-50 mM) or neuronal (50-100 mM) dysfunction in vitro, which have a wide range of downstream applications.

scholarly journals An in vitro test of the cell stretch-proliferation hypothesis of renal cyst enlargement.

1995 ◽  
Vol 6 (4) ◽  
pp. 1230-1241
Author(s):  
G A Tanner ◽  
P F McQuillan ◽  
M R Maxwell ◽  
J K Keck ◽  
J A McAteer
Keyword(s):  
Cell Proliferation ◽  
Labeling Index ◽  
In Vitro Test ◽  
Madin Darby Canine Kidney ◽  
Dna Labeling ◽  
Stimulate Cell ◽  
Darby Canine Kidney ◽  
Stimulate Cell Proliferation ◽  
Canine Kidney

In renal cystic disease, fluid accumulation within cyst lumens might stretch cyst walls and in this way stimulate cell proliferation. To test this idea, the effects of mechanical stretch on Madin-Darby canine kidney cells grown as cysts in a hydrated collagen gel or as monolayers on collagen-coated Flexcell membranes were examined. The percentage of cells synthesizing DNA (labeling index) was determined by measuring bromodeoxyuridine incorporation and counting cell numbers. The distension of single cysts for 1 h by the intraluminal injection of saline failed to produce a significant increase in labeling index. The exposure of cysts for 2.5 h to 1 mM dibutyryl cAMP + 0.1 mM isobutylmethylxanthine led to a 37% increase in luminal surface area (due to stimulated fluid secretion) and a 30% increase in labeling index. The stretch (25%) of Madin-Darby canine kidney monolayers approximately doubled the labeling index between 12 and 24 h after starting the stretch. After 48 h, the cell population density was significantly increased (P < 0.001), from 41.9 +/- 0.2 (SE; N = 12) to 48.2 +/- 0.5 (N = 12) cells/10,000 microns2. The labeling index increased linearly with applied stretch, from 7.2 +/- 0.3% (N = 36) with no stretch to 16.2 +/- 1.0% (N = 6) with 30% stretch. Stretch had to be maintained for 8 h or more to produce an increase in labeling index at 18 h. No evidence was obtained for the release of a diffusible growth factor by stretched monolayers. The increase in labeling index induced by stretch was unaffected by 50 microM gadolinium, a stretch-activated channel blocker, but was abolished by 5 micrograms/mL cytochalasin B, an actin microfilament-disrupting agent. It was concluded that prolonged stretch stimulates renal epithelial cells to synthesize DNA. This supports the idea that increased wall tension in renal cysts may stimulate cell proliferation and, thereby, may contribute to cyst enlargement.

Sign in / Sign up

Export Citation Format

Share Document