scholarly journals Effect of Chitin Nanofibrils on Biocompatibility and Bioactivity of the Chitosan-Based Composite Film Matrix Intended for Tissue Engineering

Materials ◽  
2019 ◽  
Vol 12 (11) ◽  
pp. 1874 ◽  
Author(s):  
Natalia V. Smirnova ◽  
Konstantin A. Kolbe ◽  
Elena N. Dresvyanina ◽  
Sergey F. Grebennikov ◽  
Irina P. Dobrovolskaya ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Composite Materials ◽  
Physicochemical Properties ◽  
Composite Film ◽  
Mechanical Stability ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Bioactive Properties ◽  
Optimal Balance

This paper discusses the mechanical and physicochemical properties of film matrices based on chitosan, as well as the possibility of optimizing these properties by adding chitin nanofibrils. It is shown that with the introduction of chitin nanofibrils as a filler, the mechanical stability of the composite materials increases. By varying the concentration of chitin nanofibrils, it is possible to obtain a spectrum of samples with different bioactive properties for the growth of human dermal fibroblasts. Film matrices based on the nanocomposite of chitosan and 5 wt % chitin nanofibrils have an optimal balance of mechanical and physicochemical properties and bioactivity in relation to the culture of human dermal fibroblasts.

scholarly journals Polycaprolactone-Chitin Nanofibrous Mats as Potential Scaffolds for Tissue Engineering

2012 ◽  
Vol 2012 ◽  
pp. 1-9 ◽  
Author(s):  
Min Sup Kim ◽  
Sang Jun Park ◽  
Bon Kang Gu ◽  
Chun-Ho Kim
Keyword(s):  
Electron Microscopy ◽  
Tissue Engineering ◽  
Contact Angles ◽  
Dermal Fibroblasts ◽  
Human Dermal Fibroblasts ◽  
Water Contact ◽  
Mean Diameter ◽  
Poly Caprolactone ◽  
Scanning Electron

We describe here the preparation of poly(caprolactone) (PCL)-chitin nanofibrous mats by electrospinning from a blended solution of PCL and chitin dissolved in a cosolvent, 1,1,1,3,3,3-hexafluoro-2-propanol and trifluoroacetic acid. Scanning electron microscopy showed that the neutralized PCL-chitin nanofibrous mats were morphologically stable, with a mean diameter of340.5±2.6 nm, compared with a diameter of524.2±12.1 nm for PCL mats. The nanofibrous mats showed decreased water contact angles as the proportion of chitin increased. However, the tensile properties of nanofibrous mats containing30~50% (wt/wt) chitin were enhanced compared with PCL-only mats.In vitrostudies showed that the viability of human dermal fibroblasts (HDFs) for up to 7 days in culture was higher on composite (OD value:1.42±0.09) than on PCL-only (0.51±0.14) nanofibrous mats, with viability correlated with chitin concentration. Together, our results suggest that PCL-chitin nanofibrous mats can be used as an implantable substrate to modulate HDF viability in tissue engineering.

scholarly journals 3D printing of bioreactors in tissue engineering: A generalised approach

PLoS ONE ◽  
2020 ◽  
Vol 15 (11) ◽  
pp. e0242615
Author(s):  
Marius Gensler ◽  
Anna Leikeim ◽  
Marc Möllmann ◽  
Miriam Komma ◽  
Susanne Heid ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
3D Printing ◽  
Mechanical Stability ◽  
Test Procedure ◽  
Dermal Fibroblasts ◽  
Geometrical Parameters ◽  
Full Potential ◽  
Fused Filament Fabrication ◽  
Mechanical Methods ◽  
Tissue Construct

3D printing is a rapidly evolving field for biological (bioprinting) and non-biological applications. Due to a high degree of freedom for geometrical parameters in 3D printing, prototype printing of bioreactors is a promising approach in the field of Tissue Engineering. The variety of printers, materials, printing parameters and device settings is difficult to overview both for beginners as well as for most professionals. In order to address this problem, we designed a guidance including test bodies to elucidate the real printing performance for a given printer system. Therefore, performance parameters such as accuracy or mechanical stability of the test bodies are systematically analysed. Moreover, post processing steps such as sterilisation or cleaning are considered in the test procedure. The guidance presented here is also applicable to optimise the printer settings for a given printer device. As proof of concept, we compared fused filament fabrication, stereolithography and selective laser sintering as the three most used printing methods. We determined fused filament fabrication printing as the most economical solution, while stereolithography is most accurate and features the highest surface quality. Finally, we tested the applicability of our guidance by identifying a printer solution to manufacture a complex bioreactor for a perfused tissue construct. Due to its design, the manufacture via subtractive mechanical methods would be 21-fold more expensive than additive manufacturing and therefore, would result in three times the number of parts to be assembled subsequently. Using this bioreactor we showed a successful 14-day-culture of a biofabricated collagen-based tissue construct containing human dermal fibroblasts as the stromal part and a perfusable central channel with human microvascular endothelial cells. Our study indicates how the full potential of biofabrication can be exploited, as most printed tissues exhibit individual shapes and require storage under physiological conditions, after the bioprinting process.

scholarly journals The enhanced osteogenesis and osteointegration of 3-DP PCL scaffolds via structural and functional optimization using collagen networks

RSC Advances ◽  
2018 ◽  
Vol 8 (56) ◽  
pp. 32304-32316 ◽  
Author(s):  
Jinbing Wang ◽  
Chucheng Lin ◽  
Xin Gao ◽  
Zhiwei Zheng ◽  
Mimgming Lv ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Biological Activity ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Mechanical Stability ◽  
Scaffold Design ◽  
Engineering Applications ◽  
Functional Optimization ◽  
Optimal Balance

Optimal balance between biological activity and mechanical stability should be meticulously considered during scaffold design for bone tissue engineering applications.

Alginate Scaffolds with Modified Micro Pores for Tissue Engineering Applications

2015 ◽  
Vol 749 ◽  
pp. 457-460
Author(s):  
Bon Kang Gu ◽  
Sang Jun Park ◽  
Min Sup Kim ◽  
Chun Ho Kim
Keyword(s):  
Tissue Engineering ◽  
Pore Size ◽  
Tissue Regeneration ◽  
Dermal Fibroblasts ◽  
Swelling Kinetics ◽  
Human Dermal Fibroblasts ◽  
Kinetics Analysis ◽  
Swelling Rate

In this study, we developed the porous alginate (AL) scaffolds with modified pores size and distributions to actively control tissue regeneration. An addition of 5 and 10% (v/v) butanol to AL solution was effective to control pores structures of AL scaffolds. Especially, increased amount of butanol induced that proportion of smaller pores (size of around 5~10 μm) on AL scaffolds increased. Using swelling kinetics analysis, we confirmed that micro pore modified AL scaffolds show faster swelling rate than pristine scaffolds. During in vitro study, the enhanced viability and proliferation of human dermal fibroblasts (HDFs) were observed by the pore size and distribution from micro pore modified AL scaffolds. However, AL scaffolds added 10 % butanol with excessive proportion of smaller pores induced the decreased viability of HDFs for 7 days. From our results, AL scaffolds with modified pores structures represent a potential implants to control biological in vitro and in vivo functions in a variety of tissue engineering.

A New Approach to Cartilage Tissue Engineering Using Human Dermal Fibroblasts Seeded on Three-Dimensional Polymer Scaffolds

1998 ◽  
Vol 530 ◽  
Author(s):  
S. B. Nicoll ◽  
A. Wedrychowska ◽  
N. R. Smith ◽  
R. S. Bhatnagar
Keyword(s):  
Tissue Engineering ◽  
Lactic Acid ◽  
Articular Cartilage ◽  
Cartilage Tissue Engineering ◽  
Three Dimensional ◽  
Dermal Fibroblasts ◽  
High Density ◽  
Cartilage Tissue ◽  
Human Dermal Fibroblasts ◽  
Polymer Scaffolds

AbstractCurrent methods for correcting articular cartilage defects are limited by a scarcity of cartilage cells. Here we describe a novel method for the conversion of human dermal fibroblasts to chondrocyte-like cells and the potential application of this methodology to cartilage tissue engineering. Human neonatal foreskin fibroblasts were seeded on two-dimensional, tissue culture polystyrene (TCPS) in high density micromass cultures in the presence of staurosporine (50-200 nM), a protein kinase C (PKC) inhibitor, and lactic acid (40 mM) to induce functional hypoxia. Dermal fibroblasts were similarly cultured on three-dimensional polymer scaffolds composed of a non-woven polyglycolic acid (PGA) fiber mesh reinforced in a dilute solution of poly(L-lactic acid) (PLLA). At 24 hours, northern analysis revealed a staurosporine dose-dependent increase in aggrecan core protein expression in lactate-treated micromass cultures on TCPS, while type I collagen gene expression was virtually abolished in all cultures supplemented with staurosporine. The cells in these cultures displayed a rounded, cobblestone-shaped morphology typical of differentiated chondrocytes (most pronounced at 200 n.M staurosporine and 40 mM lactate), and were organized into nodules which stained positively with Alcian blue. When seeded on PGA/PLLA matrices under identical conditions as described for TCPS, a chondrocyte-like morphology was observed in cultures treated with lactate and staurosporine in contrast to the flattened sheets of fibroblast-like cells seen in untreated controls. Taken together, the above findings suggest that staurosporine treatment coupled with high density micromass culture in the presence of lactate induces chondrogenic differentiation in human dermal fibroblasts, and that these cells may be used in concert with three-dimensional polymer scaffolds for the repair of articular cartilage lesions.

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