scholarly journals Three-Dimensional Bioprinting of Cartilage by the Use of Stem Cells: A Strategy to Improve Regeneration

Materials ◽  
2018 ◽  
Vol 11 (9) ◽  
pp. 1749 ◽  
Author(s):  
Livia Roseti ◽  
Carola Cavallo ◽  
Giovanna Desando ◽  
Valentina Parisi ◽  
Mauro Petretta ◽  
...  
Keyword(s):  
Stem Cells ◽  
Cartilage Tissue Engineering ◽  
Three Dimensional ◽  
Life Quality ◽  
Chemical Properties ◽  
Cartilage Tissue ◽  
Layer By Layer ◽  
Search Terms ◽  
Layer Deposition ◽  
Fine Control

Cartilage lesions fail to heal spontaneously, leading to the development of chronic conditions which worsen the life quality of patients. Three-dimensional scaffold-based bioprinting holds the potential of tissue regeneration through the creation of organized, living constructs via a “layer-by-layer” deposition of small units of biomaterials and cells. This technique displays important advantages to mimic natural cartilage over traditional methods by allowing a fine control of cell distribution, and the modulation of mechanical and chemical properties. This opens up a number of new perspectives including personalized medicine through the development of complex structures (the osteochondral compartment), different types of cartilage (hyaline, fibrous), and constructs according to a specific patient’s needs. However, the choice of the ideal combination of biomaterials and cells for cartilage bioprinting is still a challenge. Stem cells may improve material mimicry ability thanks to their unique properties: the immune-privileged status and the paracrine activity. Here, we review the recent advances in cartilage three-dimensional, scaffold-based bioprinting using stem cells and identify future developments for clinical translation. Database search terms used to write this review were: “articular cartilage”, “menisci”, “3D bioprinting”, “bioinks”, “stem cells”, and “cartilage tissue engineering”.

scholarly journals Mechanical loading inhibits hypertrophy in chondrogenically differentiating hMSCs within a biomimetic hydrogel

2016 ◽  
Vol 4 (20) ◽  
pp. 3562-3574 ◽  
Author(s):  
E. A. Aisenbrey ◽  
S. J. Bryant
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Mechanical Loading ◽  
Cartilage Tissue Engineering ◽  
Three Dimensional ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cartilage Tissue

Three dimensional hydrogels are a promising vehicle for delivery of adult human bone-marrow derived mesenchymal stem cells (hMSCs) for cartilage tissue engineering.

Chondrogenesis from Human Placenta-Derived Mesenchymal Stem Cells in Three-Dimensional Scaffolds for Cartilage Tissue Engineering

2011 ◽  
Vol 17 (11-12) ◽  
pp. 1549-1560 ◽  
Author(s):  
Shan-hui Hsu ◽  
Tsung-Bin Huang ◽  
Shun-Jung Cheng ◽  
Su-Ying Weng ◽  
Ching-Lin Tsai ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Placenta ◽  
Cartilage Tissue Engineering ◽  
Three Dimensional ◽  
Cartilage Tissue

A three-dimensional nanofibrous scaffold for cartilage tissue engineering using human mesenchymal stem cells

Biomaterials ◽  
2005 ◽  
Vol 26 (6) ◽  
pp. 599-609 ◽  
Author(s):  
Wan-Ju Li ◽  
Richard Tuli ◽  
Chukwuka Okafor ◽  
Assia Derfoul ◽  
Keith G Danielson ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Cartilage Tissue Engineering ◽  
Three Dimensional ◽  
Human Mesenchymal Stem Cells ◽  
Nanofibrous Scaffold ◽  
Cartilage Tissue

scholarly journals Stem Cells and Extrusion 3D Printing for Hyaline Cartilage Engineering

Cells ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 2
Author(s):  
Océane Messaoudi ◽  
Christel Henrionnet ◽  
Kevin Bourge ◽  
Damien Loeuille ◽  
Pierre Gillet ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
3D Printing ◽  
Hyaline Cartilage ◽  
Cartilage Tissue Engineering ◽  
Cartilage Tissue ◽  
Layer By Layer ◽  
Self Healing ◽  
Differentiation Potential ◽  
Promising Alternative

Hyaline cartilage is deficient in self-healing properties. The early treatment of focal cartilage lesions is a public health challenge to prevent long-term degradation and the occurrence of osteoarthritis. Cartilage tissue engineering represents a promising alternative to the current insufficient surgical solutions. 3D printing is a thriving technology and offers new possibilities for personalized regenerative medicine. Extrusion-based processes permit the deposition of cell-seeded bioinks, in a layer-by-layer manner, allowing mimicry of the native zonal organization of hyaline cartilage. Mesenchymal stem cells (MSCs) are a promising cell source for cartilage tissue engineering. Originally isolated from bone marrow, they can now be derived from many different cell sources (e.g., synovium, dental pulp, Wharton’s jelly). Their proliferation and differentiation potential are well characterized, and they possess good chondrogenic potential, making them appropriate candidates for cartilage reconstruction. This review summarizes the different sources, origins, and densities of MSCs used in extrusion-based bioprinting (EBB) processes, as alternatives to chondrocytes. The different bioink constituents and their advantages for producing substitutes mimicking healthy hyaline cartilage is also discussed.

scholarly journals Cartilage Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells in Three-Dimensional Silica Nonwoven Fabrics

2018 ◽  
Vol 8 (8) ◽  
pp. 1398 ◽  
Author(s):  
Shohei Ishikawa ◽  
Kazutoshi Iijima ◽  
Kohei Sasaki ◽  
Mineo Hashizume ◽  
Masaaki Kawabe ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Bone Marrow ◽  
Chondrogenic Differentiation ◽  
Cartilage Tissue Engineering ◽  
Three Dimensional ◽  
Cartilage Tissue ◽  
Nonwoven Fabrics

In cartilage tissue engineering, three-dimensional (3D) scaffolds provide native extracellular matrix (ECM) environments that induce tissue ingrowth and ECM deposition for in vitro and in vivo tissue regeneration. In this report, we investigated 3D silica nonwoven fabrics (Cellbed®) as a scaffold for mesenchymal stem cells (MSCs) in cartilage tissue engineering applications. The unique, highly porous microstructure of 3D silica fabrics allows for immediate cell infiltration for tissue repair and orientation of cell–cell interaction. It is expected that the morphological similarity of silica fibers to that of fibrillar ECM contributes to the functionalization of cells. Human bone marrow-derived MSCs were cultured in 3D silica fabrics, and chondrogenic differentiation was induced by culture in chondrogenic differentiation medium. The characteristics of chondrogenic differentiation including cellular growth, ECM deposition of glycosaminoglycan and collagen, and gene expression were evaluated. Because of the highly interconnected network structure, stiffness, and permeability of the 3D silica fabrics, the level of chondrogenesis observed in MSCs seeded within was comparable to that observed in MSCs maintained on atelocollagen gels, which are widely used to study the chondrogenesis of MSCs in vitro and in vivo. These results indicated that 3D silica nonwoven fabrics are a promising scaffold for the regeneration of articular cartilage defects using MSCs, showing the particular importance of high elasticity.

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