scholarly journals Physico-Chemical, In Vitro, and In Vivo Evaluation of a 3D Unidirectional Porous Hydroxyapatite Scaffold for Bone Regeneration

Materials ◽  
2017 ◽  
Vol 10 (1) ◽  
pp. 33 ◽  
Author(s):  
Manabu Tanaka ◽  
Hisao Haniu ◽  
Takayuki Kamanaka ◽  
Takashi Takizawa ◽  
Atsushi Sobajima ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
In Vivo Evaluation ◽  
Porous Hydroxyapatite ◽  
Physico Chemical ◽  
Hydroxyapatite Scaffold

scholarly journals In Vitro Physico-Chemical Characterization and Standardized In Vivo Evaluation of Biocompatibility of a New Synthetic Membrane for Guided Bone Regeneration

Materials ◽  
2019 ◽  
Vol 12 (7) ◽  
pp. 1186
Author(s):  
Lívia da Costa Pereira ◽  
Carlos Fernando de Almeida Barros Mourão ◽  
Adriana Terezinha Neves Novellino Alves ◽  
Rodrigo Figueiredo de Brito Resende ◽  
Marcelo José Pinheiro Guedes de Uzeda ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Chemical Properties ◽  
Chemical Characterization ◽  
Guided Bone Regeneration ◽  
Tissue Reaction ◽  
In Vivo Evaluation ◽  
Physico Chemical ◽  
Tissue Reactions

This study’s aim was to evaluate the biocompatibility and bioabsorption of a new membrane for guided bone regeneration (polylactic-co-glycolic acid associated with hydroxyapatite and β-tricalcium phosphate) with three thicknesses (200, 500, and 700 µm) implanted in mice subcutaneously. Scanning electron microscopy, X-ray diffraction, Fourier transform infrared spectroscopy, and the quantification of carbon, hydrogen and nitrogen were used to characterize the physico-chemical properties. One hundred Balb-C mice were divided into 5 experimental groups: Group 1—Sham (without implantation); Group 2—200 μm; Group 3—500 μm; Group 4—700 μm; and Group 5—Pratix®. Each group was subdivided into four experimental periods (7, 30, 60 and 90 days). Samples were collected and processed for histological and histomorphometrical evaluation. The membranes showed no moderate or severe tissue reactions during the experimental periods studied. The 500-μm membrane showed no tissue reaction during any experimental period. The 200-μm membrane began to exhibit fragmentation after 30 days, while the 500-μm and 700-µm membranes began fragmentation at 90 days. All membranes studied were biocompatible and the 500 µm membrane showed the best results for absorption and tissue reaction, indicating its potential for clinical guided bone regeneration.

scholarly journals In Vitro and In Vivo Evaluation of a Three-Dimensional Porous Multi-Walled Carbon Nanotube Scaffold for Bone Regeneration

Nanomaterials ◽  
2017 ◽  
Vol 7 (2) ◽  
pp. 46 ◽  
Author(s):  
Manabu Tanaka ◽  
Yoshinori Sato ◽  
Mei Zhang ◽  
Hisao Haniu ◽  
Masanori Okamoto ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Bone Regeneration ◽  
Three Dimensional ◽  
In Vivo Evaluation ◽  
Multi Walled Carbon Nanotube

In Vitro and In Vivo Evaluation of a nHA/PA66 Composite Membrane for Guided Bone Regeneration

2011 ◽  
Vol 22 (1-3) ◽  
pp. 263-275 ◽  
Author(s):  
Jidong Li ◽  
Yi Man ◽  
Yi Zuo ◽  
Li Zhang ◽  
Cui Huang ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Composite Membrane ◽  
Guided Bone Regeneration ◽  
In Vivo Evaluation

In vitro and in vivo evaluation of e-PTFE and alkali-cellulose membranes for guided bone regeneration

2001 ◽  
Vol 12 (1) ◽  
pp. 62-68 ◽  
Author(s):  
Luiz A. Salata ◽  
Paul V. Hatton ◽  
A. Jane Devlin ◽  
Geoffrey T. Craig ◽  
Ian M. Brook
Keyword(s):  
Bone Regeneration ◽  
Guided Bone Regeneration ◽  
In Vivo Evaluation ◽  
Alkali Cellulose ◽  
Cellulose Membranes

scholarly journals Microchannel Porous Hydroxyapatite Scaffold Promotes Osteogenic Differentiation by Altering the Expression of miRNAs

2021 ◽  
Author(s):  
Li Deng ◽  
Wei Qing ◽  
Lijuan Huang ◽  
Cong Liu ◽  
Jiajun Zheng ◽  
...  
Keyword(s):  
Osteogenic Differentiation ◽  
Expression Profiles ◽  
Differentially Expressed ◽  
Cell Junction ◽  
Biological Processes ◽  
Porous Hydroxyapatite ◽  
Differentially Expressed Mirnas ◽  
Hydroxyapatite Scaffold

Abstract Hydroxyapatite is a commonly used scaffold material for bone tissue engineering. However, the osteogenic mechanism of hydroxyapatite scaffolds remains unclear. Recently, we have prepared a hydroxyapatite scaffolds with microchannels and porous structures (HAG) which have good osteogenic effects in vitro and in vivo. In present study, we explained the mechanism of HAG scaffolds promoted the osteogenic differentiation from the perspective of miRNA differential expression. We used microarray assays to analyze the expression profiles of miRNAs from the osteogenic differentiation of hPMSCs with or without HAG; 16 miRNAs were upregulated and 29 miRNAs were downregulated between the two types of cells. And overexpression the differential miRNAs could promote the osteogenic differentiation of hPMSCs. Additionally, gene ontology analysis, pathway analysis, and miRNA-mRNA-network built were performed to reveal that the differentially expressed miRNAs participate in multiple biological processes, including cell metabolic, cell junction, cell development, differentiation, and signal transduction, among others. Furthermore, we found that these differentially expressed miRNAs connect osteogenic differentiation to processes such as axon guidance, MAPK, and TGF-beta signaling pathway. This is the first study to identify and characterize differentiational miRNAs derived from HAG-hPMSC cells.

scholarly journals Physico-chemical and Histomorphometric Evaluation of Zinc-containing Hydroxyapatite in Rabbits Calvaria

2016 ◽  
Vol 27 (6) ◽  
pp. 717-726 ◽  
Author(s):  
Alinne Azevedo Pereira da Silva Suruagy ◽  
Adriana Terezinha Neves Novellino Alves ◽  
Suelen Cristina Sartoretto ◽  
José de Albuquerque Calasans-Maia ◽  
José Mauro Granjeiro ◽  
...  
Keyword(s):  
Bone Repair ◽  
Chemical Properties ◽  
Wilcoxon Test ◽  
In Vivo Studies ◽  
Control Group ◽  
Porous Hydroxyapatite ◽  
Physico Chemical ◽  
Hydroxyapatite Scaffold ◽  
Xrd Patterns

Abstract The aim of this study was to characterize the physico-chemical properties and bone repair after implantation of zinc-containing nanostructured porous hydroxyapatite scaffold (nZnHA) in rabbits' calvaria. nZnHA powder containing 2% wt/wt zinc and stoichiometric nanostructured porous hydroxyapatite (nHA - control group) were shaped into disc (8 mm) and calcined at 550 °C. Two surgical defects were created in the calvaria of six rabbits (nZnHA and nHA). After 12 weeks, the animals were euthanized and the grafted area was removed, fixed in 10% formalin with 0.1 M phosphate buffered saline and embedded in paraffin (n=10) for histomorphometric evaluation. In addition, one sample from each group (n=2) was embedded in methylmethacrylate for the SEM and EDS analyses. The thermal treatment transformed the nZnHA disc into a biphasic implant composed of Zn-containing HA and Zn-containing β-tricalcium phosphate (ZnHA/βZnTCP). The XRD patterns for the nHA disc were highly crystalline compared to the ZnHA disc. Histological analysis revealed that both materials were biologically compatible and promoted osteoconduction. X-ray fluorescence and MEV-EDS of nZnHA confirmed zinc in the samples. Histomorphometric evaluation revealed the presence of new bone formation in both frameworks but without statistically significant differences (p>0.05), based on the Wilcoxon test. The current study confirmed that both biomaterials improve bone repair, are biocompatible and osteoconductive, and that zinc (2wt%) did not increase the bone repair. Additional in vivo studies are required to investigate the effect of doping hydroxyapatite with a higher Zn concentration.

scholarly journals In vitro and in vivo evaluation of synthetic resorbable membrane for guided bone regeneration

2019 ◽  
Author(s):  
Yusuke Sakaguchi ◽  
Kyohei Toyonaga ◽  
Yuuhiro Sakai ◽  
Emiko Arima ◽  
Shinichi Kato ◽  
...  
Keyword(s):  
Bone Regeneration ◽  
Guided Bone Regeneration ◽  
In Vivo Evaluation

A Porous Hydroxyapatite/Gelatin Nanocomposite Scaffold for Bone Tissue Repair: In Vitro and In Vivo Evaluation

2012 ◽  
Vol 23 (18) ◽  
pp. 2353-2368 ◽  
Author(s):  
Mahmoud Azami ◽  
Shima Tavakol ◽  
Ali Samadikuchaksaraei ◽  
Mehran Solati Hashjin ◽  
Nafiseh Baheiraei ◽  
...  
Keyword(s):  
Bone Tissue ◽  
Tissue Repair ◽  
In Vivo Evaluation ◽  
Porous Hydroxyapatite ◽  
Nanocomposite Scaffold

scholarly journals In Vitro and In Vivo Evaluation of Commercially Available Fibrin Gel as a Carrier of Alendronate for Bone Tissue Engineering

2017 ◽  
Vol 2017 ◽  
pp. 1-10 ◽  
Author(s):  
Beom Su Kim ◽  
Feride Shkembi ◽  
Jun Lee
Keyword(s):  
Bone Regeneration ◽  
Osteogenic Differentiation ◽  
Release Kinetics ◽  
Human Mesenchymal Stem Cells ◽  
Calvarial Defect ◽  
Defect Model ◽  
Fibrin Gel ◽  
In Vivo Evaluation

Alendronate (ALN) is a bisphosphonate drug that is widely used for the treatment of osteoporosis. Furthermore, local delivery of ALN has the potential to improve the bone regeneration. This study was designed to investigate an ALN-containing fibrin (fibrin/ALN) gel and evaluate the effect of this gel on both in vitro cellular behavior using human mesenchymal stem cells (hMSCs) and in vivo bone regenerative capacity. Fibrin hydrogels were fabricated using various ALN concentrations (10−7–10−4 M) with fibrin glue and the morphology, mechanical properties, and ALN release kinetics were characterized. Proliferation and osteogenic differentiation of and cytotoxicity in fibrin/ALN gel-embedded hMSCs were examined. In vivo bone formation was evaluated using a rabbit calvarial defect model. The fabricated fibrin/ALN gel was transparent with Young’s modulus of ~13 kPa, and these properties were not affected by ALN concentration. The in vitro studies showed sustained release of ALN from the fibrin gel and revealed that hMSCs cultured in fibrin/ALN gel showed significantly increased proliferation and osteogenic differentiation. In addition, microcomputed tomography and histological analysis revealed that the newly formed bone was significantly enhanced by implantation of fibrin/ALN gel in a calvarial defect model. These results suggest that fibrin/ALN has the potential to improve bone regeneration.

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