scholarly journals Two New Sexual Talaromyces Species Discovered in Estuary Soil in China

2021 ◽  
Vol 8 (1) ◽  
pp. 36
Author(s):  
Pei-Jie Han ◽  
Jian-Qiu Sun ◽  
Long Wang
Keyword(s):  
Phylogenetic Analyses ◽  
Fast Growth ◽  
Polyphasic Approach ◽  
Moderate Growth ◽  
Individual Sequences

In the survey of mycobiota of mudflats in China, two new sexually reproducing Talaromyces sect. Talaromyces species were discovered and studied using a polyphasic approach. These species are named here Talaromyces haitouensis (ex-type AS3.160101T) and Talaromyces zhenhaiensis (ex-type AS3.16102T). Morphologically, T. haitouensis is distinguished by moderate growth, green-yellow gymnothecia, orange-brown mycelium, and echinulate ellipsoidal ascospores. T. zhenhaiensis is characterized by fast growth, absence of sporulation, cream yellow to naphthalene yellow gymnothecia and mycelium, and smooth-walled ellipsoidal ascospores with one equatorial ridge. The two novelties are further confirmed by phylogenetic analyses based on either individual sequences of BenA, CaM, Rpb2, and ITS1-5.8S-ITS2 or the concatenated BenA-CaM-Rpb2 sequences.

scholarly journals Two New Teleomorphic Talaromyces Species Discovered From The Estuary Soil In China

2021 ◽  
Author(s):  
Pei-Jie Han ◽  
Jian-Qiu Sun ◽  
Long Wang
Keyword(s):  
New Species ◽  
New Taxa ◽  
Phylogenetic Analyses ◽  
Fast Growth ◽  
Moderate Growth ◽  
Individual Sequences ◽  
Two New Species

Abstract Two new species producing ascospores of Talaromyces sect.Talaromyces are proposed, namely, T. haitouensi (ex-type AS3.160101 T) and T. zhenhaiensis (ex-type AS3.16102 T). Morphologically, T. haitouensis is featured by moderate growth, green-yellow gymnothecia, orange-brown mycelium, and echinulate ellipsoidal ascospores. T. zhenhaiensis is characterized by fast growth, absent sporulation, cream yellow to naphthalene yellow gymnothecia and mycelium, smooth-walled ellipsoidal ascospores with one equatorial ridge. The proposal of the two new taxa is well supported by individual phylogenetic analyses based on individual sequences of BenA, CaM, Rpb2 and ITS1-5.8S-ITS2, and the concatenated BenA-CaM-Rpb2 sequence.

scholarly journals Cytoplasmic and Periplasmic Proteomic Signatures of Exponentially Growing Cells of the Psychrophilic BacteriumPseudoalteromonas haloplanktisTAC125

2010 ◽  
Vol 77 (4) ◽  
pp. 1276-1283 ◽  
Author(s):  
Boris Wilmes ◽  
Holger Kock ◽  
Susanne Glagla ◽  
Dirk Albrecht ◽  
Birgit Voigt ◽  
...  
Keyword(s):  
Protein Synthesis ◽  
Amino Acid ◽  
Growth Rates ◽  
Stress Proteins ◽  
High Capacity ◽  
Tca Cycle ◽  
Fast Growth ◽  
Natural Environments ◽  
Permissive Temperature ◽  
Moderate Growth

ABSTRACTThe psychrophilic model bacteriumPseudoalteromonas haloplanktisis characterized by remarkably fast growth rates under low-temperature conditions in a range from 5°C to 20°C. In this study the proteome of cellular compartments, the cytoplasm and periplasm, ofP. haloplanktisstrain TAC125 was analyzed under exponential growth conditions at a permissive temperature of 16°C. By means of two-dimensional protein gel electrophoresis and mass spectrometry, a first inventory of the most abundant cytoplasmic and periplasmic proteins expressed in a peptone-supplemented minimal medium was established. By this approach major enzymes of the amino acid catabolism of this marine bacterium could be functionally deduced. The cytoplasmic proteome showed a predominance of amino acid degradation pathways and tricarboxylic acid (TCA) cycle enzymes but also the protein synthesis machinery. Furthermore, high levels of cold acclimation and oxidative stress proteins could be detected at this moderate growth temperature. The periplasmic proteome was characterized by a significant abundance of transporters, especially of highly expressed putative TonB-dependent receptors. This high capacity for protein synthesis, efficient amino acid utilization, and substrate transport may contribute to the fast growth rates of the copiotrophic bacteriumP. haloplanktisin its natural environments.

Pseudocercospora dypsidis sp. nov. (Mycosphaerellaceae) on Dypsis lutescens leaves in Thailand

Phytotaxa ◽  
2020 ◽  
Vol 474 (3) ◽  
pp. 218-234
Author(s):  
YI-JYUN CHEN ◽  
RUVISHIKA S. JAYAWARDENA ◽  
CHITRABHANU S. BHUNJUN ◽  
DULANJALEE L. HARISHCHANDRA ◽  
KEVIN D. HYDE
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Single Gene ◽  
Morphological Characteristics ◽  
Polyphasic Approach

A cercosporoid fungus associated with leaf lesions of Dypsis lutenscens (Arecaceae) was observed in Chiang Rai, Thailand. This study describes the new species Pseudocercospora dypsidis based on morphological characteristics and phylogenetic analyses from three gene regions, ITS, TEF-1α, and ACT. Single gene analyses are generally insufficient for identification of Pseudocercospora species, or for segregation of other genera in the Pseudocercospora complex. A list is provided of all Pseudocercospora species known from Thailand and the need to confirm their identifications by a polyphasic approach is stressed.

scholarly journals Phylogenetic and evolutionary relationships of RubisCO and the RubisCO-like proteins and the functional lessons provided by diverse molecular forms

2008 ◽  
Vol 363 (1504) ◽  
pp. 2629-2640 ◽  
Author(s):  
F. Robert Tabita ◽  
Thomas E Hanson ◽  
Sriram Satagopan ◽  
Brian H Witte ◽  
Nathan E Kreel
Keyword(s):  
Active Site ◽  
Molecular Basis ◽  
Inorganic Carbon ◽  
Phylogenetic Analyses ◽  
Model Systems ◽  
Molecular Forms ◽  
Rubp Carboxylase ◽  
Individual Sequences ◽  
Bona Fide ◽  
Convenient Model

Ribulose 1,5-bisphosphate (RuBP) carboxylase/oxygenase (RubisCO) catalyses the key reaction by which inorganic carbon may be assimilated into organic carbon. Phylogenetic analyses indicate that there are three classes of bona fide RubisCO proteins, forms I, II and III, which all catalyse the same reactions. In addition, there exists another form of RubisCO, form IV, which does not catalyse RuBP carboxylation or oxygenation. Form IV is actually a homologue of RubisCO and is called the RubisCO-like protein (RLP). Both RubisCO and RLP appear to have evolved from an ancestor protein in a methanogenic archaeon, and comprehensive analyses indicate that the different forms (I, II, III and IV) contain various subgroups, with individual sequences derived from representatives of all three kingdoms of life. The diversity of RubisCO molecules, many of which function in distinct milieus, has provided convenient model systems to study the ways in which the active site of this protein has evolved to accommodate necessary molecular adaptations. Such studies have proven useful to help provide a framework for understanding the molecular basis for many important aspects of RubisCO catalysis, including the elucidation of factors or functional groups that impinge on RubisCO carbon dioxide/oxygen substrate discrimination.

scholarly journals Tree inhabiting gnomoniaceous species from China, with Cryphogonomonia gen. nov. proposed

MycoKeys ◽  
2020 ◽  
Vol 69 ◽  
pp. 71-89
Author(s):  
Qin Yang ◽  
Ning Jiang ◽  
Cheng-Ming Tian
Keyword(s):  
Leaf Spot ◽  
Woody Plants ◽  
Phylogenetic Analyses ◽  
Polyphasic Approach ◽  
Forest Trees ◽  
Gene Sequences ◽  
Tree Diseases ◽  
Wide Range ◽  
The Family ◽  
Cultural Data

Species of Gnomoniaceae are commonly associated with leaf spot diseases of a wide range of plant hosts worldwide. During our investigation of fungi associated with tree diseases in China, several gnomoniaceous isolates were recovered from symptomatic branches and leaves on different woody plants in the Fagaceae, Pinaceae, and Salicaceae families. These isolates were studied by applying a polyphasic approach including morphological, cultural data, and phylogenetic analyses of partial ITS, LSU, tef1, rpb2 and tub2 gene sequences. As a result, three species were identified with characters fitting into the family Gnomoniaceae. One of these species is described herein as Cryphognomonia pinigen. et sp. nov., characterized by developed pseudostromata and ascospores with obvious hyaline sheath; Gnomoniopsis xunwuensissp. nov. is illustrated showing sympodially branched conidiophore, oval or fusiform conidia; and one known species, Plagiostoma populinum. The current study improves the understanding of gnomoniaceous species causing diebacks and leaf spot on ecological and economic forest trees.

Morphological and phylogenetic insights reveal Cucurbitaria berberidicola (Cucurbitariaceae, Pleosporales) as a new species from Uzbekistan

Phytotaxa ◽  
2021 ◽  
Vol 518 (1) ◽  
pp. 1-13
Author(s):  
MICHÉE A. APPADOO ◽  
DHANUSHKA N. WANASINGHE ◽  
YUSUFJON GAFFOROV ◽  
K.W. THILINI CHETHANA ◽  
AZIZ ABDURAZAKOV ◽  
...  
Keyword(s):  
New Species ◽  
Molecular Phylogeny ◽  
Phylogenetic Analyses ◽  
Polyphasic Approach ◽  
Clear Distinction ◽  
Morphological Examination ◽  
Mountain Areas ◽  
Generic Type ◽  
One New Species ◽  
A New Species

A survey of microfungi in the mountain areas of Uzbekistan revealed several specimens of cucurbitaria-like taxa. In this paper, one new species of Cucurbitaria is described based on a polyphasic approach, including morphological examination and molecular phylogeny. We compared the sexual morph (ascomata, asci, and ascospores) of the specimens collected with known species of Cucurbitaria and carried out multi loci (LSU, ITS, rpb2 and tef1-α) phylogenetic analyses. Cucurbitaria berberidicola sp. nov. is morphologically closely related to Cucurbitaria berberidis (the generic type) and C. oromediterranea. The sequence-based analyses verified a clear distinction, hence supporting the introduction of the new species.

A polyphasic approach (metabolomics, morphological and molecular analyses) in the systematics of Cladobotryum species in Greece

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
N Milic ◽  
S Kostidis ◽  
A Stavrou ◽  
Z Gonou-Zagou ◽  
VN Kouvelis ◽  
...  
Keyword(s):  
Polyphasic Approach ◽  
Molecular Analyses

Ellipsomyxa ariusi sp. nov. (Myxosporea: Ceratomyxidae), a new myxosporean infecting the gallbladder of threadfin sea catfish Arius arius in India

2020 ◽  
Vol 142 ◽  
pp. 83-97
Author(s):  
A Chandran ◽  
PU Zacharia ◽  
TV Sathianandan ◽  
NK Sanil
Keyword(s):  
Phylogenetic Analyses ◽  
Type Specimen ◽  
Ssu Rdna ◽  
Fish Host ◽  
Present Species ◽  
Epithelial Layer ◽  
Histopathological Changes ◽  
Mucus Production ◽  
High Bootstrap ◽  
A New Species

The present study describes a new species of myxosporean, Ellipsomyxa ariusi sp. nov., infecting the gallbladder of the threadfin sea catfish Arius arius (Hamilton, 1822). E. ariusi sp. nov. is characterized by bivalvular, ellipsoid or elongate-oval myxospores with smooth spore valves and a straight suture, arranged at an angle to the longitudinal spore axis. Mature myxospores measured 10.1 ± 0.8 µm in length, 6.8 ± 0.5 µm in width and 7.7 ± 0.7 µm in thickness. Polar capsules are equal in size and oval to pyriform in shape. They are positioned at an angle to the longitudinal myxospore axis and open in opposite directions. Polar capsules measured 2.8 ± 0.3 µm in length and 2.5 ± 0.4 µm in width; polar filaments formed 4-5 coils, and extended to 32.2 ± 2.1 µm in length. Monosporic and disporic plasmodial stages attached to the wall of gallbladder. Molecular analysis of the type specimen generated a 1703 bp partial SSU rDNA sequence (MN892546), which was identical to the isolates from 3 other locations. In phylogenetic analyses, genus Ellipsomyxa appeared monophyletic and E. ariusi sp. nov. occupied an independent position in maximum likelihood and Bayesian inference trees with high bootstrap values. The overall prevalence of infection was 54.8% and multiway ANOVA revealed that it varied significantly with location, year, season, sex and size of the fish host. Histopathological changes associated with E. ariusi sp. nov. infection included swelling, vacuolation and detachment of epithelial layer, reduced mucus production and altered consistency and colour of bile. Based on the morphologic, morphometric and molecular differences with known species of Ellipsomyxa, and considering differences in host and geographic locations, the present species is treated as new and the name Ellipsomyxa ariusi sp. nov. is proposed.

Diversification of the North American Doellingeria-Eucephalus Clade (Astereae: Asteraceae) Inferred from Molecular and Morphological Evidence

2019 ◽  
Vol 44 (4) ◽  
pp. 930-942
Author(s):  
Geraldine A. Allen ◽  
Luc Brouillet ◽  
John C. Semple ◽  
Heidi J. Guest ◽  
Robert Underhill
Keyword(s):  
North American ◽  
Sequence Data ◽  
Phylogenetic Analyses ◽  
Sister Group ◽  
Morphological Evidence ◽  
South American ◽  
New Combinations ◽  
The North ◽  
Hybridization Event ◽  
Ancient Hybridization

Abstract—Doellingeria and Eucephalus form the earliest-diverging clade of the North American Astereae lineage. Phylogenetic analyses of both nuclear and plastid sequence data show that the Doellingeria-Eucephalus clade consists of two main subclades that differ from current circumscriptions of the two genera. Doellingeria is the sister group to E. elegans, and the Doellingeria + E. elegans subclade in turn is sister to the subclade containing all remaining species of Eucephalus. In the plastid phylogeny, the two subclades are deeply divergent, a pattern that is consistent with an ancient hybridization event involving ancestral species of the Doellingeria-Eucephalus clade and an ancestral taxon of a related North American or South American group. Divergence of the two Doellingeria-Eucephalus subclades may have occurred in association with northward migration from South American ancestors. We combine these two genera under the older of the two names, Doellingeria, and propose 12 new combinations (10 species and two varieties) for all species of Eucephalus.

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