Ellipsomyxa ariusi sp. nov. (Myxosporea: Ceratomyxidae), a new myxosporean infecting the gallbladder of threadfin sea catfish Arius arius in India

2020 ◽  
Vol 142 ◽  
pp. 83-97
Author(s):  
A Chandran ◽  
PU Zacharia ◽  
TV Sathianandan ◽  
NK Sanil
Keyword(s):  
Phylogenetic Analyses ◽  
Type Specimen ◽  
Ssu Rdna ◽  
Fish Host ◽  
Present Species ◽  
Epithelial Layer ◽  
Histopathological Changes ◽  
Mucus Production ◽  
High Bootstrap ◽  
A New Species

The present study describes a new species of myxosporean, Ellipsomyxa ariusi sp. nov., infecting the gallbladder of the threadfin sea catfish Arius arius (Hamilton, 1822). E. ariusi sp. nov. is characterized by bivalvular, ellipsoid or elongate-oval myxospores with smooth spore valves and a straight suture, arranged at an angle to the longitudinal spore axis. Mature myxospores measured 10.1 ± 0.8 µm in length, 6.8 ± 0.5 µm in width and 7.7 ± 0.7 µm in thickness. Polar capsules are equal in size and oval to pyriform in shape. They are positioned at an angle to the longitudinal myxospore axis and open in opposite directions. Polar capsules measured 2.8 ± 0.3 µm in length and 2.5 ± 0.4 µm in width; polar filaments formed 4-5 coils, and extended to 32.2 ± 2.1 µm in length. Monosporic and disporic plasmodial stages attached to the wall of gallbladder. Molecular analysis of the type specimen generated a 1703 bp partial SSU rDNA sequence (MN892546), which was identical to the isolates from 3 other locations. In phylogenetic analyses, genus Ellipsomyxa appeared monophyletic and E. ariusi sp. nov. occupied an independent position in maximum likelihood and Bayesian inference trees with high bootstrap values. The overall prevalence of infection was 54.8% and multiway ANOVA revealed that it varied significantly with location, year, season, sex and size of the fish host. Histopathological changes associated with E. ariusi sp. nov. infection included swelling, vacuolation and detachment of epithelial layer, reduced mucus production and altered consistency and colour of bile. Based on the morphologic, morphometric and molecular differences with known species of Ellipsomyxa, and considering differences in host and geographic locations, the present species is treated as new and the name Ellipsomyxa ariusi sp. nov. is proposed.

scholarly journals Taxonomy and SSU rDNA-Based Phylogeny of Two Heterotrich Ciliates (Ciliophora, Heterotrichea) Collected From Subtropical Wetlands of China, Including the Description of a New Species, Linostomella pseudovorticella n. sp.

2021 ◽  
Vol 12 ◽  
Author(s):  
Didi Jin ◽  
Xuetong Zhao ◽  
Tingting Ye ◽  
Jie Huang ◽  
Alan Warren ◽  
...  
Keyword(s):  
New Species ◽  
Food Web ◽  
Phylogenetic Analyses ◽  
Small Subunit ◽  
Ssu Rdna ◽  
Microbial Food Web ◽  
Model Organisms ◽  
Rdna Sequences ◽  
The Family ◽  
A New Species

The Heterotrichea Stein, 1859 are a group of ciliated protists (single-celled eukaryotes) that occur in a wide variety of aquatic habitat where they play important roles in the flow of nutrients and energy within the microbial food web. Many species are model organisms for research in cytology and regenerative biology. In the present study, the morphology and phylogeny of two heterotrich ciliates, namely, Linostomella pseudovorticella n. sp. and Peritromus kahli Villeneuve-Brachon, 1940, collected from subtropical wetlands of China, were investigated using morphological and molecular methods. L. pseudovorticella n. sp. differs from its only known congener, Linostomella vorticella Ehrenberg, 1833 Aescht in Foissner et al., 1999, by having more ciliary rows (48–67, mean about 56 vs. 26–51, mean about 42) and its small-subunit (SSU) rDNA sequence, which shows a 15-bp divergence. Although P. kahli has been reported several times in recent decades, its infraciliature has yet to be described. A redescription and improved diagnosis of this species based on a combination of previous and present data are here supplied. Phylogenetic analyses based on SSU rDNA sequences revealed that the genus Linostomella is positioned within Condylostomatidae, and Peritromidae is sister to Climacostomidae with relatively low support, and the family Spirostomidae is the root branch of the class Heterotrichea.

Lecanographa atlantica (Arthoniales, Lecanographaceae), a widespread and conspicuous but still undescribed lichen-forming fungus

Phytotaxa ◽  
2020 ◽  
Vol 472 (2) ◽  
pp. 147-158
Author(s):  
Damien Ertz ◽  
Pieter P. G. Van den Boom
Keyword(s):  
New Species ◽  
Mediterranean Region ◽  
Phylogenetic Analyses ◽  
Atlantic Coast ◽  
Volcanic Rocks ◽  
Type Specimen ◽  
Cape Verde ◽  
European Continent ◽  
Widespread Distribution ◽  
A New Species

Lecanographa farinosa was considered to be a lichen inhabiting coastal rocks of the Mediterranean region and to possess psoromic and conpsoromic acids as major secondary metabolites. A revision of its type specimen from Germany proved that the species has been misunderstood. In this paper, L. farinosa is shown to have a thallus containing confluentic and 2’-O-methylmicrophyllinic acids and to have a different ecology and distribution. A revision of previous reports of L. farinosa from continental Portugal and the study of recent specimens of Lecanographa cf. farinosa from the Azores, Canary Islands, Cape Verde and continental Portugal revealed a new species described as L. atlantica. Moreover, reports of L. dialeuca from France (Brittany) and of L. subgrumulosa from the Azores are considered misidentifications of L. atlantica. This latter has thus a widespread distribution ranging from the Azores to the Atlantic coast of the European continent where it inhabits volcanic rocks near the sea. Lecanographa atlantica is characterized by a whitish to cream coloured thallus, usually rounded or oblong to shortly lirelliform and white pruinose ascomata with a widely exposed hymenial disc and thin margin, (3–)5–7-septate ascospores of (16–)18.9–23(–26) × (3–)3.4–4(–4.5) µm, and a chemistry including at least 2’-O-methylperlatolic acid but lacking confluentic and 2’-O-methylmicrophyllinic acids. Psoromic acid is sometimes present in L. atlantica but its origin is unclear. Phylogenetic analyses using nuLSU sequences place L. atlantica as sister to L. hypothallina.

A new species of African Snake-Eyed Skink (Scincidae: Panaspis) from Ethiopia

Zootaxa ◽  
2020 ◽  
Vol 4779 (2) ◽  
pp. 190-200
Author(s):  
TIMOTHY J. COLSTON ◽  
R. ALEXANDER PYRON ◽  
AARON M. BAUER
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Type Specimen ◽  
Species Group ◽  
Sub Saharan Africa ◽  
Cryptic Diversity ◽  
Molecular Phylogenetic ◽  
Sub Saharan ◽  
Phylogenetic Revision ◽  
A New Species

A recent molecular phylogenetic revision of the snake-eyed skinks (genus Panaspis Cope, 1868) uncovered extensive cryptic diversity, including several new species from throughout sub-Saharan Africa. Here, we describe one of these from Ethiopia as Panaspis annettesabinae sp. nov. This description is based on a previous molecular phylogeny and morphological, scalation, and coloration data collected from the type specimen. Phylogenetic analyses place the species alone in what we term the P. annettesabinae species group from Ethiopia. This group forms the sister lineage to a large southern African radiation and suggests a potential northern origin for much of the extant diversity of Panaspis. Many new taxa have recently been discovered in the genus and region, and there are several historical records of Panaspis from elsewhere in Ethiopia. Thus, we suggest that the range of this new species (known only from a single specimen at present) may be much larger, and that additional undescribed species may exist in northern sub-Saharan Africa. 

scholarly journals Monsanima tinguaensis (Apocynaceae), an enigmatic new species from Atlantic rainforest

Phytotaxa ◽  
2014 ◽  
Vol 173 (3) ◽  
pp. 196 ◽  
Author(s):  
Uiara Catharina Soares Silva ◽  
Renata Gabrielle Pinheiro Santos ◽  
Alessandro Rapini ◽  
Jorge Fontella-Pereira ◽  
Sigrid Liede-Schumann
Keyword(s):  
New Species ◽  
Floral Morphology ◽  
Phylogenetic Analyses ◽  
Type Specimen ◽  
Northeastern Brazil ◽  
Atlantic Rainforest ◽  
Southeastern Brazil ◽  
Campos Rupestres ◽  
A New Species ◽  
Janeiro State

A new species of Asclepiadoideae (Apocynaceae) with enigmatic taxonomic position was found in the Atlantic rainforest of Rio de Janeiro State, southeastern Brazil. To ascertain its affinities within the Asclepiadeae, we performed phylogenetic analyses using the plastid regions trnT-L and rps16. Our results strongly support the new species close to the Orthosiinae, but do not support any generic relationship for this species. Based on the floral morphology, especially the corona lobes, which are highly fused, and curved inward at the top, covering the gynostegium, the new species is assigned to Monsanima. This genus is sister to the other Orthosiinae and included so far only one species narrowly endemic to the campos rupestres of Bahia, in northeastern Brazil. The new species, Monsanima tinguaensis, occurs in a protected area, Tinguá Biological Reserve. However, it is known only by the type specimen and is probably vulnerable to extinction.

Morphology and SSU rDNA sequences of four cyrtophorian ciliates from China, with description of a new species (Protista, Ciliophora, Phyllopharyngea)

Zootaxa ◽  
2019 ◽  
Vol 4664 (2) ◽  
pp. 206-220
Author(s):  
CONGCONG WANG ◽  
ZHISHUAI QU ◽  
XIAOZHONG HU
Keyword(s):  
Phylogenetic Analyses ◽  
Ssu Rdna ◽  
Rrna Genes ◽  
Rrna Gene ◽  
Ssu Rrna ◽  
Rdna Sequences ◽  
Rdna Sequencing ◽  
Close Relationship ◽  
First Time ◽  
A New Species

Four cyrtophorian ciliates, Chlamydodon pararoseus sp. n., Chlamydodon bourlandi Qu et al., 2018, Dysteria crassipes Claparède & Lachmann, 1859, and Dysteria monostyla (Ehrenberg, 1838) Kahl, 1931, isolated from coastal waters off China, were investigated using morphological and SSU rDNA sequencing techniques. C. pararoseus sp. n. can be recognized by having a kidney-shaped body, a complete cross-striated band, 44–51 somatic kineties, and 11–14 nematodesmal rods. The other three species were re-described based on new populations. Additionally, the SSU rRNA genes of C. pararoseus sp. n. and D. monostyla were sequenced for the first time. Phylogenetic analyses inferred from SSU rRNA gene sequences reveal that C. pararoseus sp. n. has a close relationship with C. bourlandi and C. mnemosyne Gong et al., 2005 within the genus Chlamydodon; and D. monostyla and D. crassipes cluster with its congeners, which confirms their generic assignments. 

A New Species of Vietnamophryne with an Extended Description of Vietnamophryne orlovi

2021 ◽  
Vol 28 (6) ◽  
pp. 355-368
Author(s):  
Thanh Vinh Nguyen ◽  
Chung Van Hoang ◽  
Jiang Jianping ◽  
Nikolai L. Orlov ◽  
Hoa Thi Ninh ◽  
...  
Keyword(s):  
New Species ◽  
National Park ◽  
Phylogenetic Analyses ◽  
Type Specimen ◽  
Morphological Characters ◽  
Colour Pattern ◽  
Ecological Specialization ◽  
Single Male ◽  
Northern Vietnam ◽  
A New Species

We describe Vietnamophryne cuongi sp. nov., a new species of microhylid frog from northern Vietnam, based on morphological and molecular differences. Although superficially similar to remaining Vietnamophryne members, the new species differs by a number of diagnostic morphological characters, such as body size, habitus, head dimensions, finger and toe morphology, skin texture, as well as colour pattern. Phylogenetic analyses based on a fragment of the mitochondrial 16S gene place the new species to be sister to V. orlovi, from which it differed by 2.4% genetic divergence. We also describe the first female known of V. orlovi, collected in the forest of Phia Oac-Phia Den National Park, Cao Bang Province. The latter species, for which we provide an extended description herein, was recently described based on the single male type specimen only from the same site in northern Vietnam. Vietnamophryne cuongi sp. nov. is only known from three specimens, two adult females and a juvenile, from the evergreen tropical forest of Ba Vi National Park, Hanoi. As such, it is likely to be at high risk of habitat loss. Considering its high ecological specialization and the small known distribution range of the new species, we propose Endangered as IUCN Red List status.

Ceriodaphnia smirnovi (Crustacea: Cladocera), a new species from the Mediterranean Region, and a phylogenetic analysis of the commonest species

Zootaxa ◽  
2021 ◽  
Vol 4974 (1) ◽  
pp. 1-46
Author(s):  
MIGUEL ALONSO ◽  
ANNA N. NERETINA ◽  
MARC VENTURA
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Mediterranean Region ◽  
Phylogenetic Analyses ◽  
Biological Species ◽  
Present Species ◽  
Nominal Species ◽  
Species Groups ◽  
The Mediterranean ◽  
A New Species

Species of the genus Ceriodaphnia Dana, 1853 (Crustacea: Cladocera: Daphniidae) are present in the plankton of many freshwater bodies all around the World. However, the taxonomy of the genus is still poorly developed. Most species have been described in the nineteenth century and data on their morphology and distribution should be revised according to current standards. Currently, due to lack of reliable morphological and genetic data, almost all nominal species of Ceriodaphnia are considered as species groups rather than separate biological species, complicating the detection and description of new species of the genus. Here, we redescribe the morphology of Ceriodaphnia quadrangula (O.F. Müller, 1785) based on material close to the type locality in Denmark, and describe a new species of Ceriodaphnia from the Mediterranean region. Also, we provide a phylogenetic analysis using existing and novel sequences of mitochondrial DNA of the commonest species of the genus. Ceriodaphnia smirnovi sp. nov. can be easily distinguished from C. quadrangula via presence of the head pore and the structure of the second thoracic limb, the postabdomen and the ephippium. From other species of Ceriodaphnia known to date C. smirnovi sp. nov. can also be separated on ephippium structure and some other fine morphological features. Although distribution range of C. smirnovi sp. nov. must be clarified in the future based on analysis of more sampling points, most likely, it is restricted to the Mediterranean region. The phylogenetic analyses showed high divergence among 16 different clades (mean clade separation of 13±2.3 % (mean ± SD) and 20.7±2.1% for 16S and COI respectively). These clades belonged to 11 described species and 5 undescribed. The high intraspecific variability of some species indicates the possible existence of more undescribed species within present species groups. 

Bivalvulidan (Myxozoa: Myxosporea) parasites of damselfishes with description of twelve novel species from Australia's Great Barrier Reef

Parasitology ◽  
2008 ◽  
Vol 135 (10) ◽  
pp. 1165-1178 ◽  
Author(s):  
N. L. GUNTER ◽  
R. D. ADLARD
Keyword(s):  
Novel Species ◽  
Phylogenetic Analyses ◽  
Single Species ◽  
Small Subunit ◽  
Ssu Rdna ◽  
Fish Host ◽  
Multiple Infections ◽  
Rdna Sequences ◽  
Molecular Phylogenetic ◽  
Host Taxon

SUMMARYBivalvulidan parasites from the gall bladder of 31 species of damselfishes (family Pomacentridae) were examined for their taxonomic identity and their relatedness to other species of myxozoans. This paper describes 11 novel ceratomyxid species and a novel Myxidium sp. Each species is characterized morphologically and small subunit (SSU) rDNA sequences were used in molecular phylogenetic analyses. Five pomacentrid species were found to harbour multiple infections of bivalvulidan species. One species of Ceratomyxa and Myxidium were found to infect more than a single species of damselfish. Phylogenetic analyses revealed there has been no radiation of ceratomyxids that can be associated with the fish host taxon and that Myxidium queenslandicus n.sp. was more closely related to Zschokkella mugilis and Ellipsomyxa gobii than other members of the genus Myxidium.

scholarly journals Molecular record for the first authentication of Isaria cicadae from Vietnam

2021 ◽  
Vol 16 (1) ◽  
pp. 711-718
Author(s):  
Thuan Duc Lao ◽  
Hanh Van Trinh ◽  
Loi Vuong ◽  
Luyen Tien Vu ◽  
Thuy Ai Huyen Le ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Genomic Dna ◽  
Morphological Analysis ◽  
Phylogenetic Analyses ◽  
Neighbor Joining ◽  
Pcr Assay ◽  
Parsimony Method ◽  
Maximum Parsimony Method ◽  
Cordyceps Cicadae ◽  
High Bootstrap

Abstract The entomopathogenic fungus T011, parasitizing on nymph of Cicada, collected in the coffee garden in Dak Lak Province, Vietnam, was preliminarily morphologically identified as Isaria cicadae, belonged to order Hypocreales and family Clavicipitaceae. To ensure the authenticity of T011, phylogenetic analysis of the concatenated set of multiple genes including ITS, nrLSU, nrSSU, Rpb1, and Tef1 was applied to support the identification. Genomic DNA was isolated from dried sample T011. The PCR assay sequencing was applied to amplify ITS, nrLSU, nrSSU, Rpb1, and Tef1 gene. For phylogenetic analysis, the concatenated data of both target gens were constructed with MEGAX with a 1,000 replicate bootstrap based on the neighbor-joining, maximum likelihood, maximum parsimony method. As the result, the concatenated data containing 62 sequences belonged to order Hypocreales, families Clavicipitaceae, and 2 outgroup sequences belonged to order Hypocreales, genus Verticillium. The phylogenetic analysis results indicated that T011 was accepted at subclade Cordyceps and significantly formed the monophyletic group with referent Cordyceps cicadae (Telemorph of Isaria cicadae) with high bootstrap value. The phylogenetically analyzed result was strongly supported by our morphological analysis described as the Isaria cicadae. In summary, phylogenetic analyses based on the concatenated dataset were successfully applied to strengthen the identification of T011 as Isaria cicadae.

scholarly journals Molecular and phylogenetic analysis reveals new diversity of Dunaliella salina from hypersaline environments

2021 ◽  
pp. 1-11
Author(s):  
Andrea Highfield ◽  
Angela Ward ◽  
Richard Pipe ◽  
Declan C. Schroeder
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Analysis ◽  
Dunaliella Salina ◽  
Phylogenetic Analyses ◽  
Ssu Rdna ◽  
Lsu Rdna ◽  
Hypersaline Environments ◽  
Rdna Its ◽  
Β Carotene ◽  
Selection Of

Abstract Twelve hyper-β carotene-producing strains of algae assigned to the genus Dunaliella salina have been isolated from various hypersaline environments in Israel, South Africa, Namibia and Spain. Intron-sizing of the SSU rDNA and phylogenetic analysis of these isolates were undertaken using four commonly employed markers for genotyping, LSU rDNA, ITS, rbcL and tufA and their application to the study of Dunaliella evaluated. Novel isolates have been identified and phylogenetic analyses have shown the need for clarification on the taxonomy of Dunaliella salina. We propose the division of D. salina into four sub-clades as defined by a robust phylogeny based on the concatenation of four genes. This study further demonstrates the considerable genetic diversity within D. salina and the potential of genetic analyses for aiding in the selection of prospective economically important strains.

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