Novel Carbohydrate Specificity of Monoclonal Antibody 91.9H Prepared against Human Colonic Sulfomucin: Recognition of Sulfo-Lewis a Structure

1998 ◽  
Vol 253 (2) ◽  
pp. 374-381 ◽  
Author(s):  
Hitomi Tsuiji ◽  
Joe C. Hong ◽  
Young S. Kim ◽  
Yuzuru Ikehara ◽  
Hisashi Narimatsu ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Carbohydrate Specificity ◽  
Lewis A

scholarly journals Reinvestigation of Carbohydrate Specificity of EBCA-1 Monoclonal Antibody Used for the Detection of Candida Mannan

2021 ◽  
Vol 7 (7) ◽  
pp. 504
Author(s):  
Vadim B. Krylov ◽  
Arsenii S. Solovev ◽  
Ilya A. Puchkin ◽  
Dmitry V. Yashunsky ◽  
Anna V. Antonets ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Invasive Candidiasis ◽  
Carbohydrate Specificity ◽  
Coated Plate ◽  
Sera Samples ◽  
Immune Assay

Monoclonal antibody EBCA-1 is used in the sandwich immune assay for the detection of circulating Candida mannan in blood sera samples for the diagnosis of invasive candidiasis. To reinvestigate carbohydrate specificity of EBCA-1, a panel of biotinylated oligosaccharides structurally related to distinct fragments of Candida mannan were loaded onto a streptavidin-coated plate to form a glycoarray. Its use demonstrated that EBCA-1 recognizes the trisaccharide β-Man-(1→2)-α-Man-(1→2)-α-Man and not homo-α-(1→2)-linked pentamannoside, as was reported previously.

scholarly journals mAb Das-1 recognizes 3’-Sulfated Lewis A/C, which is aberrantly expressed during metaplastic and oncogenic transformation of several gastrointestinal Epithelia

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0261082
Author(s):  
Jeffrey W. Brown ◽  
Koushik K. Das ◽  
Vasilios Kalas ◽  
Kiron M. Das ◽  
Jason C. Mills
Keyword(s):  
Monoclonal Antibody ◽  
Risk Stratification ◽  
Complete Loss ◽  
Ductal Adenocarcinoma ◽  
High Grade ◽  
Glycan Array ◽  
Multiple Organs ◽  
Gastrointestinal Lesions ◽  
Direct Elisa ◽  
Lewis A

Introduction Multiple previous studies have shown the monoclonal antibody Das-1 (formerly called 7E12H12) is specifically reactive towards metaplastic and carcinomatous lesions in multiple organs of the gastrointestinal system (e.g. Barrett’s esophagus, intestinal-type metaplasia of the stomach, gastric adenocarcinoma, high-grade pancreatic intraepithelial neoplasm, and pancreatic ductal adenocarcinoma) as well as in other organs (bladder and lung carcinomas). Beyond being a useful biomarker in tissue, mAb Das-1 has recently proven to be more accurate than current paradigms for identifying cysts harboring advanced neoplasia. Though this antibody has been used extensively for clinical, basic science, and translational applications for decades, its epitope has remained elusive. Methods In this study, we chemically deglycosylated a standard source of antigen, which resulted in near complete loss of the signal as measured by western blot analysis. The epitope recognized by mAb Das-1 was determined by affinity to a comprehensive glycan array and validated by inhibition of a direct ELISA. Results The epitope recognized by mAb Das-1 is 3’-Sulfo-Lewis A/C (3’-Sulfo-LeA/C). 3’-Sulfo-LeA/C is broadly reexpressed across numerous GI epithelia and elsewhere during metaplastic and carcinomatous transformation. Discussion 3’-Sulfo-LeA/C is a clinically important antigen that can be detected both intracellularly in tissue using immunohistochemistry and extracellularly in cyst fluid and serum by ELISA. The results open new avenues for tumorigenic risk stratification of various gastrointestinal lesions.

scholarly journals Reinvestigation of carbohydrate specificity of EB-A2 monoclonal antibody used in the immune detection of Aspergillus fumigatus galactomannan

Heliyon ◽  
2019 ◽  
Vol 5 (1) ◽  
pp. e01173 ◽  
Author(s):  
Vadim B. Krylov ◽  
Arsenii S. Solovev ◽  
Dmitry A. Argunov ◽  
Jean-Paul Latgé ◽  
Nikolay E. Nifantiev
Keyword(s):  
Monoclonal Antibody ◽  
Aspergillus Fumigatus ◽  
Carbohydrate Specificity ◽  
Immune Detection

Target-selective photo-degradation of a sialyl Lewis a (sLea) conjugate and photo-cytotoxicity against sLea positive cancer cells using an anthraquinone-antibody hybrid

MedChemComm ◽  
2016 ◽  
Vol 7 (6) ◽  
pp. 1224-1228 ◽  
Author(s):  
Daisuke Takahashi ◽  
Takashi Nagao ◽  
Shota Sotokawa ◽  
Kazunobu Toshima
Keyword(s):  
Monoclonal Antibody ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Cancer Cells ◽  
Photo Degradation ◽  
Sialyl Lewis ◽  
Sialyl Lewis A ◽  
Lewis A

A purpose-designed anthraquinone–monoclonal antibody (anti-sialyl Lewis A (sLea) mAb) hybrid 6 selectively bound to and effectively degraded the target glycoprotein, HSA (human serum albumin)–sLea conjugate 4.

scholarly journals 3'-Sulfated Lewis A is a Biomarker for Metaplastic and Oncogenic Transformation of Several Gastrointestinal Epithelia

2021 ◽  
Author(s):  
Jeffrey Wade Brown ◽  
Koushik Kumar Das ◽  
Vasilios Kalas ◽  
Kiron Das ◽  
Jason C Mills
Keyword(s):  
Monoclonal Antibody ◽  
Risk Stratification ◽  
Complete Loss ◽  
Ductal Adenocarcinoma ◽  
High Grade ◽  
Glycan Array ◽  
Multiple Organs ◽  
Gastrointestinal Lesions ◽  
Direct Elisa ◽  
Lewis A

Introduction: Multiple previous studies have shown the monoclonal antibody Das-1 (formerly called 7E12H12) specifically recognizes metaplastic and carcinomatous lesions in multiple organs of the gastrointestinal system (e.g. Barrett's esophagus, intestinal-type metaplasia of the stomach, gastric adenocarcinoma, high-grade pancreatic intraepithelial neoplasm, and pancreatic ductal adenocarcinoma) as well as in other organs (bladder and lung carcinomas). Beyond being a useful biomarker in tissue, mAb Das-1 has recently proven to be more accurate than current paradigms for identifying cysts harboring advanced neoplasia. Though this antibody has been used extensively for clinical, basic science, and translational applications for decades, its epitope has remained elusive. Methods: In this study, we chemically deglycosylated a standard source of antigen, which resulted in near complete loss of the signal as measured by western blot analysis. The epitope recognized by mAb Das-1 was determined by affinity to a comprehensive glycan array and validated by inhibition of a direct ELISA. Results: The epitope recognized by mAb Das-1 is 3'-Sulfo-Lewis A (3'-Sulfo-LeA). 3'-Sulfo-LeA is broadly reexpressed across numerous GI epithelia and elsewhere only after metaplastic and carcinomatous transformation. Discussion: 3'-Sulfo-LeA is a clinically important antigen that can be detected both intracellularly in tissue using immunohistochemistry and extracellularly in cyst fluid and serum by ELISA. The results open new avenues for tumorigenic risk stratification of various gastrointestinal lesions.

The Antitumor Monoclonal Antibody MOv2 Recognizes the Lewis A Hapten

Hybridoma ◽  
1988 ◽  
Vol 7 (2) ◽  
pp. 129-139 ◽  
Author(s):  
FLAVIO LEONI ◽  
JOHN L. MAGNANI ◽  
SILVIA MIOTTI ◽  
SILVANA CANEVARI ◽  
MARINA PASQUALI ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Lewis A

A web-like network of type vi collagen in human skin is revealed by immuno-electron microscopy

1988 ◽  
Vol 46 ◽  
pp. 382-383
Author(s):  
Douglas R. Keene ◽  
Robert W. Glanville ◽  
Eva Engvall
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibody ◽  
Human Skin ◽  
Basement Membranes ◽  
Primary Antibody ◽  
Fat Cells ◽  
Secondary Antibody ◽  
Type Vi Collagen ◽  
Dermal Matrix ◽  
Immuno Electron Microscopy

A mouse monoclonal antibody (5C6) prepared against human type VI collagen (1) has been used in this study to immunolocalize type VI collagen in human skin. The enbloc method used involves exposing whole tissue pieces to primary antibody and 5 nm gold conjugated secondary antibody before fixation, and has been described in detail elsewhere (2).Biopsies were taken from individuals ranging in age from neonate to 65 years old. By immuno-electron microscopy, type VI collagen is found to be distributed as a fine branching network closely associated with (but not attached to) banded collagen fibrils containing types I and III collagen (Fig. 1). It appears to enwrap fibers, to weave between individual fibrils within a fiber, and to span the distance separating fibers, creating a “web-like network” which entraps fibers within deep papillary and reticular dermal layers (Fig. 2). Relative to that in the dermal matrix, the concentration of type VI collagen is higher around endothelial basement membranes limiting the outer boundaries of nerves, capillaries, and fat cells (Fig. 3).

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